---++ Day 1: Plating the Mixed Population 1. Find microsatellite-containing strains in the -80°C freezer. They will be labeled with the appropriate microsatellite sequence located in the rhamnose operon (eg. "(CA)<sub>12</sub>"). See below for the list of microsatellites. ---++ Day 2: Picking Colonies ---++ Day 3: Passaging Colonies | *Number* | *Sequence* | *Position* | *Fluorescent Tag?* | | 1 | (CA)<sub>6</sub> | _rhaT-rhaD_ | | | 2 | (CA)<sub>12</sub> | _rhaT-rhaD_ | | | 3 | (TA)<sub>12</sub> | _rhaT-rhaD_ | | | 4 | (A)<sub>24</sub> | _rhaT-rhaD_ | | | 5 | (AGA)<sub>8</sub> | _rhaT-rhaD_ | | | 6 | (GGA)<sub>8</sub> | _rhaT-rhaD_ | | | 7 | (G)<sub>9</sub> | _rhaT-rhaD_ | | | 8 | (G)<sub>12</sub> | _rhaT-rhaD_ | | -- Main.LindseyWolf - 18 May 2011
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Topic revision: r1 - 2011-05-18 - LindseyWolf