Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	191	1,611	C→T	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	191	1,611	0	C	T	84.5%	114.0 / 17.1	58	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand):  ref base C (6/3);  new base T (19/30);  total (25/33)
Fisher's exact test for biased strand distribution p-value = 1.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.99e-01

GCTTTTTGACTGTTTGCTGATGCAGTGGCAGAAGCAGCCGCCGCACTTGCAGAACCAGCTGCAGCACTCTCGCTTTCGGCTGCTGCATCCTGACTGCTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTTGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTG  >  191/1515‑1711                                                                                                 |                                                                                                     gCTTTTTGACTGTTGGCTGATGCAGTGGCAGAAGAAGCCGCCGCACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTtttt                                                                                                  <  1:1062566/101‑1 (MQ=255) gCTTTTTGACTGTTGGCTGATGCAGTGGCAGAAGAAGCCGCCGCACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTtttt                                                                                                  <  1:1561670/101‑1 (MQ=255)  cTTTTTGACTGTTGGCTGATGCAGTGGCAGAAGAAGCCGCCGCACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTc                                                                                                 <  2:762976/101‑1 (MQ=255)      ttGACTGTTGGCTGATGCAGTGGCAGAAGAAGCCGCCGCACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTcgccg                                                                                             >  1:1362203/1‑101 (MQ=255)      ttGACTGTTGGCTGATGCAGTGGCAGAAGAAGCCGCCGCACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTcgccg                                                                                             <  1:1275731/101‑1 (MQ=255)       tGACTGTTGGCTGATGCAGTGGCAGAAGAAGCCGCCGCACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTcgccgc                                                                                            >  1:888214/1‑101 (MQ=255)       tGACTGTTGGCTGATGCAGTGGCAGAAGAAGCCGCCGCACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTcgccgc                                                                                            <  2:944508/101‑1 (MQ=255)         aCTGTTGGCTGATGCAGTGGCAGAAGAAGCCGCCGCACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAg                                                                                          <  2:1644338/101‑1 (MQ=255)          cTGTTGGCTGATGCAGGGGCAGAAGAAGCCGCCGCACTTGCAGAACCAGCTGCGGCCCTCTCACTTTGGGCCGCTTCATCCTGACTGTTTTTCGCCGCAGt                                                                                         <  2:1583912/101‑1 (MQ=255)             ttggCTGATGCAGTGGCAGAAGAAGCCGCCGCACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTc                                                                                      <  2:1104751/98‑1 (MQ=25)             ttggCTGATGCAGTGGCAGAAGAAGCCGCCGCACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTc                                                                                      <  1:1246806/98‑1 (MQ=25)             ttggCTGATGCAGTGGCAGAAGAAGCCGCCGCACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTc                                                                                      >  1:414609/4‑101 (MQ=25)              tggCTGATGCAGTGGCAGAAGAAGCCGCCGCACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCg                                                                                     >  1:897062/3‑101 (MQ=25)                 cTGATGCAGTGGCAGAAGAAGCCGCCGCACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTg                                                                                  >  2:402342/1‑101 (MQ=25)                  tGATGCAGTGGCAGAAGAAGCCGCCGCACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTgg                                                                                 >  1:633231/1‑101 (MQ=25)                   gATGCAGTGGCAGAAGAAGCCGCCGCACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGc                                                                                <  1:694332/101‑1 (MQ=25)                     tGCAGTGGCAGAAGAAGCCGCCGCACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCtt                                                                              >  1:163311/1‑101 (MQ=25)                      gCAGTGGCAGAAGAAGCCGCCGCACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCttt                                                                             <  1:415422/101‑1 (MQ=25)                            gCAGAAGAAGCCGCCGCACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCAtt                                                                       <  2:501253/101‑1 (MQ=21)                             cAGAAGAAGCCGCCGCACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCAttc                                                                      >  1:5962/1‑100 (MQ=21)                               gaagaaGCCGCCGCACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGt                                                                    >  1:673271/1‑101 (MQ=18)                               gaagaaGCCGCCGCACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGt                                                                    <  2:810679/101‑1 (MQ=18)                                aagaagCCGCCGCACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGtt                                                                   <  2:983242/101‑1 (MQ=18)                                 agaagcCGCCGCACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGttt                                                                  <  1:619794/98‑1 (MQ=18)                                     gccgccGCACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCt                                                              <  2:1525231/101‑1 (MQ=18)                                           gcACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCtt                                                        <  2:295074/101‑1 (MQ=17)                                            cACTTGCAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTc                                                       <  2:538872/101‑1 (MQ=17)                                                  cAGAACCAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGcc                                                 <  2:505075/101‑1 (MQ=14)                                                       ccAGCTGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTctg                                            <  1:433886/101‑1 (MQ=14)                                                        cggCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCATTCGTTTCGCTTGTTTTCGCTGCCGCctgg                                           >  1:681485/3‑101 (MQ=11)                                                          gCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCATTCGTTTCGCTTGTTTTCGCTGCCGCctggct                                         <  1:1323834/101‑1 (MQ=11)                                                          gCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCATTCGTTTCGCTTGTTTTCGCTGCCGCctggct                                         <  1:311560/101‑1 (MQ=11)                                                           cTGCGGCACTCTCGCTGTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTctggctg                                        <  1:957028/101‑1 (MQ=14)                                                            tGCGGCACTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTctggctgg                                       >  2:745272/1‑101 (MQ=14)                                                             gccgcaCTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCATTCGTTTCGCTTGTTTTCGCTGCCGCCTGGCTGGa                                      >  2:524441/4‑101 (MQ=11)                                                               ggcaCTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACt                                    <  2:1400008/100‑1 (MQ=14)                                                                gcaCTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACtt                                   <  2:1593306/101‑1 (MQ=14)                                                                gcaCTCTCGCTTTCGGCTGCTGCAGCCTCACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCATTCGTTTCGCTTGTTTTCGCTGCCGCCTGGCTGGACtt                                   <  2:438723/101‑1 (MQ=11)                                                                 caCTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACttt                                  <  1:1649020/101‑1 (MQ=14)                                                                 caCTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCATTCGTTTCGCTTGTTTTCGCTGCCGCCTGGCTGGACttt                                  >  1:1268286/1‑101 (MQ=14)                                                                  aCTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTg                                 >  1:978532/1‑101 (MQ=14)                                                                  aCTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTg                                 <  1:402347/101‑1 (MQ=14)                                                                  aCTCTCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTg                                 >  1:500769/1‑101 (MQ=14)                                                                  aCTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCATTCGTTTCGCTTGTTTTCGCTGCCGCCTGGCTGGACTTTg                                 <  1:1653789/101‑1 (MQ=11)                                                                  aCTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCATTCGTTTCGCTTGTTTTCGCTGCCGCCTGGCTGGACTTTg                                 >  1:568867/1‑101 (MQ=11)                                                                  aCTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCATTCGTTTCGCTTGTTTTCGCTGCCGCCTGGCTGGACTTTg                                 >  2:1090226/1‑101 (MQ=11)                                                                   ctctCGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGc                                <  2:657565/101‑1 (MQ=14)                                                                     ctcGCTTTGGGCCGCTGCATCCTGCCTGTTTTTCGCCGCAGTTTCGCTGGCTTGGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGt                              <  1:50596/101‑1 (MQ=11)                                                                     ctcGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCATTCGTTTCGCTTGTTTTCGCTGCCGCCTGGCTGGACTTTGCGt                              >  1:1313394/1‑101 (MQ=11)                                                                       cGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGGTGGACTTTGCGTTa                            >  2:107575/1‑101 (MQ=14)                                                                       cGCTTTGGGCCGCTGCATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTa                            <  1:1317147/101‑1 (MQ=14)                                                                          tttggtgcgctggATCCTGACTGTTTTCCGCCGCCGTTTCGCTTGCTTTGGCATTCGTTTCGTTGGCCTTCGATGCCGTCTGGCTGGACTTTGCGTTAGtt                         <  2:83297/93‑1 (MQ=14)                                                                            tgggCCGCTGCATCCTGGCTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTc                       <  2:280209/99‑1 (MQ=11)                                                                                 cgctgcATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCg                  <  2:1092013/100‑1 (MQ=255)                                                                                 cgctgcATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCg                  <  1:1000159/100‑1 (MQ=255)                                                                                  gctgcATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGt                 >  2:1278739/1‑101 (MQ=255)                                                                                      cATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTc             <  1:469345/101‑1 (MQ=255)                                                                                      cATCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTc             >  2:1357826/1‑101 (MQ=255)                                                                                       aTCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTNCGTTAGTTTCGCTCGTCTTCg            >  2:789041/1‑101 (MQ=255)                                                                                       aTCCTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCg            >  1:1248837/1‑101 (MQ=255)                                                                                          cTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCgctg         >  2:817168/1‑101 (MQ=255)                                                                                          cTGACTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCgctg         >  1:859790/1‑101 (MQ=255)                                                                                             aCTGTTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCGCTGCtt      >  2:439112/1‑101 (MQ=255)                                                                                                 cTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTTGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTg  >  1:699987/1‑101 (MQ=255)                                                                                                 |                                                                                                     GCTTTTTGACTGTTTGCTGATGCAGTGGCAGAAGCAGCCGCCGCACTTGCAGAACCAGCTGCAGCACTCTCGCTTTCGGCTGCTGCATCCTGACTGCTTTTCGCCGCAGTTTCGCTGGCTTTGGCATTTGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTG  >  191/1515‑1711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑