Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	178	1,428	T→A	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	178	1,428	0	T	A	100.0%	82.6 / NA	25	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (12/13);  total (12/13)

CTTAATGCCGGTGATCGTAAAGGCGCATGCGAGGCGATTCGCTGGTGGAT‑AAAGGACGGTGGACGTGATTGCCGCATTCGTTCAAATAATTGTTACGGTCAGGTTATTCGTCGTGACCAGGAGAGTGCATTAACCTGCTGGGGGATAGAACAG  >  178/1330‑1482                                                                                                    |                                                       ctgaaTGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACa                                                      <  2:1126102/98‑1 (MQ=255)  tgaaTGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAg                                                     >  2:963025/3‑101 (MQ=255)  tgaaTGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGAGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAg                                                     >  2:11726/3‑101 (MQ=255)     aTGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGtt                                                  >  2:311794/1‑101 (MQ=255)       gCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTAt                                                <  2:227805/101‑1 (MQ=255)         cGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATtc                                              <  2:1145004/101‑1 (MQ=255)          ggTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATtcg                                             <  1:879083/101‑1 (MQ=255)            tGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATtcgtc                                           >  2:221854/1‑101 (MQ=255)                 gTAAGGGTGCATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATCCATTCAAATAACTGCTACGGACAGGTTATTCGTCGTGAc                                      <  2:1143694/101‑1 (MQ=255)                   aagggtgcATGCGAGCCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCa                                    >  1:209122/7‑101 (MQ=255)                    agggtgcATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAg                                   <  1:177155/96‑1 (MQ=255)                           aTGCGAGGCGATTCGTTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGaaagcg                            >  2:1466239/1‑99 (MQ=255)                              cGAGGCGATTCGTTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCAt                         <  1:1466245/101‑1 (MQ=255)                                aGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTa                       <  2:1589734/101‑1 (MQ=255)                                    gATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCct                   >  1:272032/1‑101 (MQ=255)                                     aTTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCctg                  >  1:494602/1‑101 (MQ=255)                                      ttCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCctgt                 >  2:1495759/1‑100 (MQ=255)                                        cGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCctgttg               <  1:528466/101‑1 (MQ=255)                                          cTGGTGGATAAAAGGTTGGTGG‑CGCGCTTTCCGCATACGTTCAAATAACTGGTATGGGCAGGTTTTTCGTCGTGACCAGGAAAGCGCATTAGCCTGTTggg             <  1:414827/101‑1 (MQ=255)                                           tggtggAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCCTGTTgggg            >  2:605165/1‑101 (MQ=255)                                             gtggAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCCTGTTGGGGGa          >  1:41328/1‑101 (MQ=255)                                              tggAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCCTGTTGGGGGAt         <  2:1701175/101‑1 (MQ=255)                                                 aT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCCTGTTGGGGGATAGa      >  1:1328072/1‑101 (MQ=255)                                                  t‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCCTGTTGGGGGATAGat     <  2:1209757/101‑2 (MQ=255)                                                  t‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCCTGTTGGGGGATAGat     <  1:81095/101‑2 (MQ=255)                                                    aaaaGATGGGGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCCTGTTGGGGGATAGatc    >  2:247443/1‑99 (MQ=255)                                                      aagatggTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCCTGTTGGGGGATAGATCAg  <  2:280073/96‑1 (MQ=255)                                                      aagatggTGGGCGCGATTGCCGCATACGTTCAAATAACGGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCCTGTTGGGGGATAGATCAg  >  2:994943/6‑101 (MQ=255)                                                                                                    |                                                       CTTAATGCCGGTGATCGTAAAGGCGCATGCGAGGCGATTCGCTGGTGGAT‑AAAGGACGGTGGACGTGATTGCCGCATTCGTTCAAATAATTGTTACGGTCAGGTTATTCGTCGTGACCAGGAGAGTGCATTAACCTGCTGGGGGATAGAACAG  >  178/1330‑1482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑