Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	178	1,422	T→C	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	178	1,422	0	T	C	100.0%	104.5 / NA	30	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/16);  total (14/16)

GCGGCTTAATGCCGGTGATCGTAAAGGCGCATGCGAGGCGATTCGCTGGTGGAT‑AAAGGACGGTGGACGTGATTGCCGCATTCGTTCAAATAATTGTTACGGTCAGGTTATTCGTCGTGACCAGGAGAGTGCATTAACCTGCTGGGGGATAGAACAG  >  178/1326‑1482                                                                                                  |                                                             gCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTatg                                                          >  2:892557/1‑99 (MQ=255)  cGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATgg                                                         >  1:1451271/1‑101 (MQ=255)   ggCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATgga                                                        <  2:1119693/101‑2 (MQ=255)   ggCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATgga                                                        <  2:1576098/101‑2 (MQ=255)    gCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATggac                                                       <  1:1702997/101‑3 (MQ=255)     ctgaaTGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACa                                                      <  2:1126102/98‑1 (MQ=255)      tgaaTGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAg                                                     >  2:963025/3‑101 (MQ=255)      tgaaTGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGAGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAg                                                     >  2:11726/3‑101 (MQ=255)         aTGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGtt                                                  >  2:311794/1‑101 (MQ=255)           gCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTAt                                                <  2:227805/101‑1 (MQ=255)             cGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATtc                                              <  2:1145004/101‑1 (MQ=255)              ggTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATtcg                                             <  1:879083/101‑1 (MQ=255)                tGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATtcgtc                                           >  2:221854/1‑101 (MQ=255)                     gTAAGGGTGCATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATCCATTCAAATAACTGCTACGGACAGGTTATTCGTCGTGAc                                      <  2:1143694/101‑1 (MQ=255)                       aagggtgcATGCGAGCCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCa                                    >  1:209122/7‑101 (MQ=255)                        agggtgcATGCGAGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAg                                   <  1:177155/96‑1 (MQ=255)                               aTGCGAGGCGATTCGTTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGaaagcg                            >  2:1466239/1‑99 (MQ=255)                                  cGAGGCGATTCGTTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCAt                         <  1:1466245/101‑1 (MQ=255)                                    aGGCGATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTa                       <  2:1589734/101‑1 (MQ=255)                                        gATTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCct                   >  1:272032/1‑101 (MQ=255)                                         aTTCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCctg                  >  1:494602/1‑101 (MQ=255)                                          ttCGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCctgt                 >  2:1495759/1‑100 (MQ=255)                                            cGCTGGTGGAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCctgttg               <  1:528466/101‑1 (MQ=255)                                              cTGGTGGATAAAAGGTTGGTGG‑CGCGCTTTCCGCATACGTTCAAATAACTGGTATGGGCAGGTTTTTCGTCGTGACCAGGAAAGCGCATTAGCCTGTTggg             <  1:414827/101‑1 (MQ=255)                                               tggtggAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCCTGTTgggg            >  2:605165/1‑101 (MQ=255)                                                 gtggAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCCTGTTGGGGGa          >  1:41328/1‑101 (MQ=255)                                                  tggAT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCCTGTTGGGGGAt         <  2:1701175/101‑1 (MQ=255)                                                     aT‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCCTGTTGGGGGATAGa      >  1:1328072/1‑101 (MQ=255)                                                      t‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCCTGTTGGGGGATAGat     <  2:1209757/101‑2 (MQ=255)                                                      t‑AAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCCTGTTGGGGGATAGat     <  1:81095/101‑2 (MQ=255)                                                        aaaaGATGGGGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCCTGTTGGGGGATAGatc    >  2:247443/1‑99 (MQ=255)                                                          aagatggTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCCTGTTGGGGGATAGATCAg  <  2:280073/96‑1 (MQ=255)                                                          aagatggTGGGCGCGATTGCCGCATACGTTCAAATAACGGCTATGGACAGGTTATTCGTCGTGACCAGGAAAGCGCATTAGCCTGTTGGGGGATAGATCAg  >  2:994943/6‑101 (MQ=255)                                                                                                  |                                                             GCGGCTTAATGCCGGTGATCGTAAAGGCGCATGCGAGGCGATTCGCTGGTGGAT‑AAAGGACGGTGGACGTGATTGCCGCATTCGTTCAAATAATTGTTACGGTCAGGTTATTCGTCGTGACCAGGAGAGTGCATTAACCTGCTGGGGGATAGAACAG  >  178/1326‑1482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑