Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	158	2,269	T→G	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	158	2,269	0	T	G	100.0%	272.5 / NA	75	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (36/39);  total (36/39)

TCAACGGCACCAATCTCAATGATCTTGTGGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATTGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGAAGTCTACCAGAGATGCTTAAACAGGTAGAAATT  >  158/2171‑2365                                                                                                   |                                                                                                 tCAACGGCACCAATCTCAATGATCTTGTGGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGc                                                                                                <  1:811553/101‑1 (MQ=255)   aaCGGCACCAATCTCAATGATCTTGTGGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAg                                                                                              >  2:44553/1‑101 (MQ=255)      ggCACCAATCTCAATGATCTTGTGGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGc                                                                                           >  2:995650/1‑101 (MQ=255)       gCACCAATCTCAATGATCTTGTGGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCt                                                                                          <  2:1208105/101‑1 (MQ=255)        cACCAATCTCAATGATCTTGTGGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTc                                                                                         >  1:1677930/1‑101 (MQ=255)          ccAATCTCAATGATCTTGTGGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCAt                                                                                       <  2:1251918/101‑1 (MQ=255)           cAATCTCAATGATCTTGTGGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATa                                                                                      <  1:448128/101‑1 (MQ=255)           cAATCTCAATGATCTTGTGGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATa                                                                                      >  1:1410718/1‑101 (MQ=255)            aaTCTCAATGATCTTGTGGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAg                                                                                     <  1:90375/101‑1 (MQ=255)               ctcAATGATCTTGTGGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCgg                                                                                  >  1:886230/1‑101 (MQ=255)                tcAATGATCTTGTGGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGt                                                                                 <  2:1562389/101‑1 (MQ=255)                  aaTGATCTTGTGGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCa                                                                               >  2:1528446/1‑101 (MQ=255)                  aaTGATCTTGTGGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCa                                                                               >  2:130408/1‑101 (MQ=255)                   aTGATCTTGTGGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCAt                                                                              <  2:639893/101‑1 (MQ=255)                     gaccTTGTGGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCAttt                                                                            >  1:1430905/4‑101 (MQ=255)                     gATCTTGTGGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCAttt                                                                            >  2:620452/1‑101 (MQ=255)                      aTCTTGTGGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTa                                                                           >  2:916997/1‑101 (MQ=255)                       tCTTGTGGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTAt                                                                          <  2:1529439/101‑1 (MQ=255)                       tCTTGTGGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTAt                                                                          <  2:1671780/101‑1 (MQ=255)                       tCTTGTGGCCTTCATAGTGCACACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTAt                                                                          <  1:1448522/101‑1 (MQ=255)                        cTTGTGGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATg                                                                         >  2:728895/1‑101 (MQ=255)                          tgtgGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTc                                                                       <  1:1147752/101‑1 (MQ=255)                          tgtgGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTc                                                                       >  1:423616/1‑101 (MQ=255)                           gtgGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCa                                                                      <  1:36906/101‑1 (MQ=255)                            tgGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAg                                                                     <  2:1497790/101‑1 (MQ=255)                            tgGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAg                                                                     <  2:947853/101‑1 (MQ=255)                             ggCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGa                                                                    >  1:319228/1‑101 (MQ=255)                               ccTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACt                                                                  <  2:420962/101‑1 (MQ=255)                               ccTCCATAGTGCGCACTAATCTGGTTCATACCGGTGGTTTCGCTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACt                                                                  <  1:87981/101‑1 (MQ=255)                                cTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACtt                                                                 >  1:647163/1‑101 (MQ=255)                                cTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACtt                                                                 <  1:917067/101‑1 (MQ=255)                                 ttCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTa                                                                >  2:153039/1‑101 (MQ=255)                                   cATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATc                                                              <  2:195612/101‑1 (MQ=255)                                    aTAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCg                                                             >  2:1260248/1‑101 (MQ=255)                                    aTAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCg                                                             >  1:471039/1‑101 (MQ=255)                                          cgcACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTAc                                                       <  1:39311/101‑1 (MQ=255)                                          cgcACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTAc                                                       >  2:1024265/1‑101 (MQ=255)                                           gcACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACa                                                      >  1:1432611/1‑101 (MQ=255)                                            cACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAg                                                     <  1:1649122/101‑1 (MQ=255)                                             aCCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGt                                                    >  1:607678/1‑101 (MQ=255)                                                aaTCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCg                                                 <  1:995655/101‑1 (MQ=255)                                                aaTCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCg                                                 >  2:1330939/1‑101 (MQ=255)                                                    tGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTc                                             <  1:131433/101‑1 (MQ=255)                                                     ggTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCa                                            >  2:1674688/1‑101 (MQ=255)                                                      gTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCaa                                           <  1:1252788/101‑1 (MQ=255)                                                       ttCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCTGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAAt                                          >  1:369840/1‑101 (MQ=255)                                                       ttCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAAt                                          <  1:1386918/101‑1 (MQ=255)                                                        tCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAAtt                                         <  1:44553/101‑1 (MQ=255)                                                         cATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTa                                        >  1:1590382/1‑101 (MQ=255)                                                          aTACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTAc                                       >  1:742610/1‑101 (MQ=255)                                                          aTACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTAc                                       <  1:325623/101‑1 (MQ=255)                                                            aCCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAg                                     >  1:804689/1‑101 (MQ=255)                                                              cGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGa                                   <  2:1395891/101‑1 (MQ=255)                                                                  ggtTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGAAGTc                               <  1:801777/101‑1 (MQ=255)                                                                  ggtTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGAAGTc                               >  1:143435/1‑101 (MQ=255)                                                                   gtTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGAAGTCt                              <  2:1430899/101‑1 (MQ=255)                                                                   gtTTCGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGAAGTCt                              >  1:280554/1‑101 (MQ=255)                                                                       cGGTATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGAAGTCTACCa                          >  2:1491934/1‑101 (MQ=255)                                                                          tATCGAGAACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGAAGTCTACCagag                       <  1:620457/101‑1 (MQ=255)                                                                               agaACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGAAGTCTACCAGAGATGCt                  <  2:162486/101‑1 (MQ=255)                                                                               agaACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGAAGTCTACCAGAGATGCt                  <  2:647158/101‑1 (MQ=255)                                                                               agaACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGAAGTCTACCAGAGATGCt                  <  1:1414557/101‑1 (MQ=255)                                                                               agaACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGAAGTCTACCAGAGATGCt                  <  1:675211/101‑1 (MQ=255)                                                                                gaACGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGAAGTCTACCAGAGATGCtt                 <  1:728900/101‑1 (MQ=255)                                                                                  aCGATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGAAGTCTACCAGAGATGCTTaa               <  1:1568212/101‑1 (MQ=255)                                                                                    gATCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGAAGTCTACCAGAGATGCTTAAAc             >  1:43602/1‑101 (MQ=255)                                                                                     aTCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGAAGTCTACCAGAGATGCTTAAACa            >  2:1651625/1‑101 (MQ=255)                                                                                      tCTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGAAGTCTACCAGAGATGCTTAAACAg           <  1:1532792/101‑1 (MQ=255)                                                                                       cTGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGAAGTCTACCAGAGATGCTTAAACAgg          >  2:190341/1‑101 (MQ=255)                                                                                       cTGGCGTGTAATGGCAGTGCTCATAGCCGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGAAGTCTACCAGAGATGCTTAAACAgg          <  1:509466/101‑1 (MQ=255)                                                                                        tGGCGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGAAGTCTACCAGAGATGCTTAAACAGGt         >  2:1144091/1‑101 (MQ=255)                                                                                           cGTGTAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGAAGTCTACCAGAGATGCTTAAACAGGTAGa      >  1:222927/1‑101 (MQ=255)                                                                                             tgtAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGAAGTCTACCAGAGATGCTTAAACAGGTAGaaa    >  1:627885/1‑101 (MQ=255)                                                                                               tAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGAAGTCTACCAGAGATGCTTAAACAGGTAGAAAtt  <  2:21261/101‑1 (MQ=255)                                                                                               tAATGGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGAAGTCTACCAGAGATGCTTAAACAGGTAGAAAtt  >  1:504135/1‑101 (MQ=255)                                                                                                   |                                                                                                 TCAACGGCACCAATCTCAATGATCTTGTGGCCTTCATAGTGCGCACCAATCTGGTTCATACCGGTGGTTTCGGTATCGAGAACGATCTGGCGTGTAATTGCAGTGCTCATAGCGGTCATTTATGTCAGACTTATCGTTTTACAGTTCGATTCAATTACAGGAAGTCTACCAGAGATGCTTAAACAGGTAGAAATT  >  158/2171‑2365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑