Predicted mutation
evidence seq id position mutation annotation gene description
RA 84 5,678 C→T intergenic (–/–)  /  –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*845,6780CT95.6% 131.4 / ‑1.6 45intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (23/20);  total (24/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.37e-01

CAAACAGTGCGCCGTGGCGCGCGGTCATTGTTGCGTTAATACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGCGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTCAACCAGCACTTCAATCTTGCCGCCGCTGGCTTTACGCCCAAACAGGCGCGCCGGGATCA  >  84/5583‑5777
                                                                                               |                                                                                                   
cAAACAGTGCGCCGTGGCGCGCGGTCATTGTTGCGTTAATACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAata                                                                                                <  2:591977/101‑1 (MQ=255)
cAAACAGTGCGCCGTGGCGCGCGGTCATTGTTGCGTTAATACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAata                                                                                                <  1:139559/101‑1 (MQ=255)
 aaaCAGTGCGCCGTGGCGCGCGGTCATTGTTGCGTTAATACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAatat                                                                                               <  2:1141217/101‑1 (MQ=255)
     aGTGCGCCGTGGCGCGCGGTCATTGTTGCGTTAATACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATgcgc                                                                                           <  2:779114/101‑1 (MQ=255)
       tGCGCCGTGGCGCGCGGTCATTGTTGCGTTAATACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCaa                                                                                         <  2:469275/101‑1 (MQ=255)
             gTGGCGCGCGGTCATTGTTGCGTTAATACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGc                                                                                   >  2:335516/1‑101 (MQ=255)
              tgagtcagtgTCATTGTTGCGTTAATACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCg                                                                                  >  2:396600/10‑101 (MQ=255)
              tGGCGCGCGGTCATTGTTGCGTTAATACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCg                                                                                  <  2:355704/101‑1 (MQ=255)
              tGGCGCGCGGTCATTGTTGCGTTAATACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGCGAATATGCGCAAGAATGCg                                                                                  <  2:881133/101‑1 (MQ=255)
               ggCGCGCGGTCATTGTTGCGTTAATACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGt                                                                                 <  1:159179/101‑1 (MQ=255)
                 cgcgcgGTCATTGTTGCGTTAATACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGttt                                                                               <  1:1004544/101‑1 (MQ=255)
                 cgcgcgGTCATTGTTGCGTTAATACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGttt                                                                               <  1:270991/101‑1 (MQ=255)
                  gcgcgGTCATTGTTGCGTTAATACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTg                                                                              <  1:955717/101‑1 (MQ=255)
                     cgGTCATTGTTGCGTTAATACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTgtcg                                                                           >  2:868968/1‑101 (MQ=255)
                        tCATTGTTGCGTTAATACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGtcgtcg                                                                        >  1:1068714/1‑101 (MQ=255)
                        tCATTGTTGCGTTAATACTTTCGTCATCGCCCACCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGtcgtcg                                                                        >  2:835977/1‑101 (MQ=255)
                          aTTGTTGCGTTAATACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAg                                                                      >  2:1338891/1‑101 (MQ=255)
                          aTTGTTGCGTTAATACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAg                                                                      <  2:1298935/101‑1 (MQ=255)
                            tgttgCGTTAATACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGCTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGGTTGTCGTCGAGCa                                                                    >  2:1023575/1‑101 (MQ=255)
                             gttgCGTTAATACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCAt                                                                   >  2:254871/1‑101 (MQ=255)
                               tgCGTTAATACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATcc                                                                 >  1:1651197/1‑101 (MQ=255)
                                  gTTAATACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGtt                                                              >  1:884951/1‑101 (MQ=255)
                                   ttAATACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTc                                                             >  1:874881/1‑101 (MQ=255)
                                       tgcTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTCAAcc                                                         <  1:1695012/99‑1 (MQ=255)
                                       tACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTCAAcc                                                         <  2:291798/101‑1 (MQ=255)
                                           ttCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTCAACCAGCa                                                     >  2:1279275/1‑101 (MQ=255)
                                             cGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTCAACCAGCACt                                                   >  2:58832/1‑101 (MQ=255)
                                               tCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTCAACCAGCACTTc                                                 >  2:931411/1‑101 (MQ=255)
                                                cATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGCGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTCAACCAGCACTTCa                                                >  2:954443/1‑101 (MQ=255)
                                                 aTCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTCAACCAGCACTTCaa                                               >  2:829465/1‑101 (MQ=255)
                                                   cGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTCAACCAGCACTTCAATc                                             <  1:1402312/101‑1 (MQ=255)
                                                    gCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAAAATGCGTTTGTCGTCGAGCATCCGTTCAACCAGCACTTCAAACt                                            <  2:1087328/101‑1 (MQ=255)
                                                     cccAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTCAACCAGCACTTCAATCtt                                           >  2:1327666/1‑101 (MQ=255)
                                                     cccAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTCAACCAGCACTTCAATCtt                                           <  1:821541/101‑1 (MQ=255)
                                                           agcagTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTCAACCAGCACTTCAATCTTgccgcc                                     <  1:369526/101‑1 (MQ=255)
                                                               gTTCTGCGCCAGGTTTTGGCGCTTTCGCGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTCAACCAGCACTTCAATCTTGCCGCCGCTg                                 >  2:264974/1‑101 (MQ=255)
                                                               gTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTCAACCAGCACTTCAATCTTGCCGCCGCTg                                 >  1:253288/1‑101 (MQ=255)
                                                                  cTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTCAACCAGCACTTCAATCTTGCCGCCGCTGGCt                              <  1:1569104/101‑1 (MQ=255)
                                                                  cTGCGCCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTCAACCAGCACTTCAATCTTGCCGCCGCTGGCt                              >  1:428126/1‑101 (MQ=255)
                                                                     cgcCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTCAACCAGCACTTCAATCTTGCCGCCGCTGGCTTTa                           >  2:1481845/1‑101 (MQ=255)
                                                                      gcCAGGTTTTGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTCAACCAGCACTTCAATCTTGCCGCCGCTGGCTTTAc                          <  1:1023580/101‑1 (MQ=255)
                                                                              ttGGCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTCAACCAGCACTTCAATCTTGCCGCCGCTGGCTTTACGCCCAAAc                  <  1:1707359/101‑1 (MQ=255)
                                                                                ggCGCTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTCAACCAGCACTTCAATCTTGCCGCCGCTGGCTTTACGCCCAAACAg                >  2:1040452/1‑101 (MQ=255)
                                                                                    cTTTCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTCAACCAGCACTTCAATCTTGCCGCCGCTGGCTTTACGCCCAAACAGgcgc            <  1:920406/101‑1 (MQ=255)
                                                                                       tCGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTCAACCAGCACTTCAATCTTGCCGCCGCTGGCTTTACGCCCAAACAGGCGCGcc         >  1:542715/1‑101 (MQ=255)
                                                                                        cGAGGCGTGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGGTCAACCCGCACTCAAATCTTGCCGCCGCTGGCTTTACGCCCAAACAGGCGCGCCg        >  2:1462565/1‑101 (MQ=255)
                                                                                          aGGCGTTAATATGCGCAAGAATGCGTTTTTCGTCGACCATCCGTTCAACCAGCGCTTCAATCTTGCCGCCGCTGGCTTTACGCCCAAACAGGCGCGCCggg      <  2:1205335/101‑1 (MQ=255)
                                                                                              gcgAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTCAACCAGCACTTCAATCTTGCCGCCGCTGGCTTTACGCCCAAACAGGCGCGCCGGGATCa  <  2:386552/101‑1 (MQ=255)
                                                                                               |                                                                                                   
CAAACAGTGCGCCGTGGCGCGCGGTCATTGTTGCGTTAATACTTTCGTCATCGCCCAGCAGCAGTTCTGCGCCAGGTTTTGGCGCTTTCGAGGCGCGAATATGCGCAAGAATGCGTTTGTCGTCGAGCATCCGTTCAACCAGCACTTCAATCTTGCCGCCGCTGGCTTTACGCCCAAACAGGCGCGCCGGGATCA  >  84/5583‑5777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: