Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	30	20,997	A→C	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	30	20,997	0	A	C	98.7%	267.0 / ‑4.3	75	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (40/34);  total (40/35)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

TTTTCTCAACTAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATAAAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGT  >  30/20898‑21095                                                                                                    |                                                                                                   ttttCTCAACTAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATaaa                                                                                                   >  2:916012/1‑101 (MQ=255)  tttCTCAACTAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATacaa                                                                                                  <  2:344305/101‑1 (MQ=255)   ttCTCAACTAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAg                                                                                                 >  1:323446/1‑101 (MQ=255)     ctcAACTAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAggg                                                                                               >  2:1085080/1‑101 (MQ=255)     ctcAACTAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAggg                                                                                               <  1:188380/101‑1 (MQ=255)       cAACTAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGtt                                                                                             >  1:311231/1‑101 (MQ=255)       cAACTAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGtt                                                                                             >  2:266292/1‑101 (MQ=255)        aaCTAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGttt                                                                                            <  1:390552/101‑1 (MQ=255)         tcTAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTc                                                                                           >  2:394887/2‑101 (MQ=255)         aCTAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTc                                                                                           <  1:995439/101‑1 (MQ=255)           tAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCat                                                                                         <  1:549987/101‑1 (MQ=255)            aGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCata                                                                                        >  1:1572732/1‑101 (MQ=255)             gcggcgCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCatat                                                                                       <  1:696925/101‑1 (MQ=255)             gcggcgCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCCGGGAATACAAGGGTTTCatat                                                                                       <  2:1144670/101‑1 (MQ=255)              cggcgCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATAtt                                                                                      <  1:1007314/101‑1 (MQ=255)               ggcgCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTg                                                                                     >  1:1070326/1‑101 (MQ=255)               ggcgCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTg                                                                                     >  2:741591/1‑101 (MQ=255)               ggcgCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTg                                                                                     <  2:221602/101‑1 (MQ=255)                   cgGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGccc                                                                                 >  1:745062/1‑101 (MQ=255)                    ggTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCa                                                                                <  2:714142/101‑1 (MQ=255)                    ggTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCa                                                                                >  2:1081786/1‑101 (MQ=255)                     gTTAGCAGCGGAAGAAGTAACACGCTTTATTGTCCCAGGCGGTGAGCACTCCGGCGTAGACACACAGAGCCTGGGAATACAAGGGCTTCATATTGCCCCaa                                                                               >  2:1679723/1‑101 (MQ=255)                          cagcGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCAtt                                                                          >  1:766184/1‑101 (MQ=255)                           agcGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCAttt                                                                         <  2:1308722/101‑1 (MQ=255)                             cGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTcc                                                                       <  2:1127985/101‑1 (MQ=255)                              ggAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTccc                                                                      >  2:942808/1‑101 (MQ=255)                               gAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTcccc                                                                     >  2:1655047/1‑101 (MQ=255)                                aaGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTccccc                                                                    <  1:1125769/101‑1 (MQ=255)                                  ggCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCgg                                                                  <  1:329316/101‑1 (MQ=255)                                    cGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCg                                                                >  1:241889/1‑101 (MQ=255)                                     gttacacGCTTTATTGTCGCAGGCGGTGAGGCCTCCGGCGTAGTCACTCAGAACCTGGGAATACAAGGGTTTCATTTTGGCCCAGCCATTTCCCCCGGCGt                                                               <  2:298799/98‑1 (MQ=255)                                      tGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTg                                                              >  2:1444249/1‑101 (MQ=255)                                         cacGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCg                                                           <  1:741596/101‑1 (MQ=255)                                          acGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGt                                                          >  2:84171/1‑101 (MQ=255)                                             cTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTggg                                                       >  2:1313961/1‑101 (MQ=255)                                             cTTTATTGCCGCAGGCGGTGAGACCTCCGGCGTATTCACACAGAGCCTGTGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTggg                                                       >  2:212466/1‑101 (MQ=255)                                              tttATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGt                                                      >  1:426329/1‑101 (MQ=255)                                                 aTTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTaaa                                                   >  1:780398/1‑101 (MQ=255)                                                      cGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCAc                                              <  2:1018670/101‑1 (MQ=255)                                                      cGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCAc                                              <  1:971494/101‑1 (MQ=255)                                                      cGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCAc                                              <  1:1688899/101‑1 (MQ=255)                                                       gCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACt                                             <  1:1298331/101‑1 (MQ=255)                                                        cAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTg                                            <  2:1047445/101‑1 (MQ=255)                                                           gCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGAt                                         <  1:30953/101‑1 (MQ=255)                                                             ggTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATaa                                       >  2:737386/1‑101 (MQ=255)                                                             ggTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATaa                                       >  1:610373/1‑101 (MQ=255)                                                             ggTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATaa                                       >  2:1693951/1‑101 (MQ=255)                                                              gTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAg                                      >  2:1670500/1‑101 (MQ=255)                                                              gTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGATTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGAAAACGCACTGGATAAg                                      >  1:1563114/1‑101 (MQ=255)                                                                gagaCCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGcc                                    >  2:633207/1‑101 (MQ=255)                                                                 agaCCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCt                                   <  1:1096736/101‑1 (MQ=255)                                                                 agaCCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCt                                   <  1:1085085/101‑1 (MQ=255)                                                                   aCCTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGt                                 <  2:1151136/101‑1 (MQ=255)                                                                   aCCTCCGGCCTAGACACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTGCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGt                                 >  2:1261281/1‑101 (MQ=255)                                                                    ccTCCGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGtc                                >  1:1168497/1‑101 (MQ=255)                                                                       ccGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCa                             <  2:941466/101‑1 (MQ=255)                                                                        cGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCAc                            <  1:787727/101‑1 (MQ=255)                                                                        cGGCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCAc                            >  1:1167478/1‑101 (MQ=255)                                                                         ggCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCAGTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACt                           <  2:167737/101‑1 (MQ=255)                                                                          gCGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTc                          <  2:290378/101‑1 (MQ=255)                                                                           cGTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCg                         >  1:1087464/1‑101 (MQ=255)                                                                            gTAGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGc                        >  2:596277/1‑101 (MQ=255)                                                                              aGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGccc                      >  1:1666094/1‑101 (MQ=255)                                                                              aGTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGccc                      >  1:1154521/1‑101 (MQ=255)                                                                               gTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCt                     >  2:317749/1‑101 (MQ=255)                                                                               gTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCt                     >  2:608367/1‑101 (MQ=255)                                                                               gTCACACAGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCt                     >  1:456945/1‑101 (MQ=255)                                                                                 cacacaGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTa                   >  2:80215/1‑101 (MQ=255)                                                                                  acacaGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTaa                  <  1:1105426/101‑1 (MQ=255)                                                                                     caGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATc               >  2:786814/1‑101 (MQ=255)                                                                                     caGAGCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATc               <  1:266292/101‑1 (MQ=255)                                                                                      agagCCTGGGAATACAAGGGTTTCAAATTAGCCCAACCATTTGCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACCCGCACTTAAATCt              <  2:1010411/101‑1 (MQ=255)                                                                                       gagCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATCTg             <  1:993188/101‑1 (MQ=255)                                                                                        agCCTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATCTgg            <  1:949173/101‑1 (MQ=255)                                                                                          ccTGGGAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATCTGGCa          <  2:904364/101‑1 (MQ=255)                                                                                             gggAATACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATCTGGCAACt       <  1:1665623/101‑1 (MQ=255)                                                                                                aaTACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATCTGGCAACTTCg    <  2:494650/101‑1 (MQ=255)                                                                                                aaTACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATCTGGCAACTTCg    <  1:583997/101‑1 (MQ=255)                                                                                                aaTACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATCTGGCAACTTCg    >  1:46390/1‑101 (MQ=255)                                                                                                 aTACAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATCTGGCAACTTCgg   >  1:78246/1‑101 (MQ=255)                                                                                                  tAAAAGGGTTTCATGTTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGt  <  1:916017/101‑1 (MQ=255)                                                                                                    |                                                                                                   TTTTCTCAACTAGCGGCGCGGTTAGCAGCGGAAGGCGTGACACGCTTTATTGTCGCAGGCGGTGAGACCTCCGGCGTAGTCACACAGAGCCTGGGAATAAAAGGGTTTCATATTGGCCCAACCATTTCCCCCGGCGTGCCGTGGGTAAACGCACTGGATAAGCCTGTCTCACTCGCCCTTAAATCTGGCAACTTCGGT  >  30/20898‑21095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑