Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	118	13,537	G→A	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	118	13,537	0	G	A	100.0%	180.9 / NA	52	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/20);  total (32/20)

TCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTGGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTACGCGGCGATGATGGC  >  118/13445‑13634                                                                                             |                                                                                                  tCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGc                                                                                           >  2:452310/1‑101 (MQ=255)  cACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCt                                                                                          <  1:1448287/101‑1 (MQ=255)   aCCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTg                                                                                         >  2:1612330/1‑101 (MQ=255)    ccAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGc                                                                                        >  2:79764/1‑101 (MQ=255)     cAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCg                                                                                       <  1:157365/101‑1 (MQ=255)      aaCATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGa                                                                                      >  1:344668/1‑101 (MQ=255)      aaCATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGa                                                                                      >  1:1672721/1‑101 (MQ=255)       aCATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGaa                                                                                     >  1:1424312/1‑101 (MQ=255)         aTCGCACAACTGAAAGATCCGAAGATCGCTAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAgg                                                                                   >  1:13802/1‑101 (MQ=255)         aTCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAgg                                                                                   >  1:1383462/1‑101 (MQ=255)            gCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAAGGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGc                                                                                >  2:1441599/1‑101 (MQ=255)             cacaACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCg                                                                               <  1:349223/101‑1 (MQ=255)                    gAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAacc                                                                        >  2:111570/1‑101 (MQ=255)                    gAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAacc                                                                        >  1:336779/1‑101 (MQ=255)                     aaaGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAacca                                                                       <  1:812414/101‑1 (MQ=255)                         aTCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAg                                                                   >  1:6487/1‑101 (MQ=255)                          cgtttttaTCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTCACCCTAGCTGGGGCTGCGGAGGTGCGATCAAACACCAGc                                                                  >  2:1286132/8‑101 (MQ=255)                             gAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTg                                                               >  2:539227/1‑101 (MQ=255)                              aaGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGc                                                              >  1:410412/1‑101 (MQ=255)                              aaGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGc                                                              <  2:195655/101‑1 (MQ=255)                                gATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCcg                                                            >  1:80200/1‑101 (MQ=255)                                   cGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGt                                                         >  2:610969/1‑101 (MQ=255)                                   cGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGt                                                         <  2:445642/101‑1 (MQ=255)                                       aaaCTGTTCGATACCAACGGCGACGGGAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCGGCGAAGGTGCGATCAACGACCAGCTTGCCGCGTATGa                                                     >  1:186444/1‑101 (MQ=255)                                         aCTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAAc                                                   <  1:1421915/101‑1 (MQ=255)                                           tGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTg                                                 >  1:635102/1‑101 (MQ=255)                                           tGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTg                                                 <  1:665637/101‑1 (MQ=255)                                               cGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGAccc                                             >  2:1052261/1‑101 (MQ=255)                                                gATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCa                                            <  2:325089/101‑1 (MQ=255)                                                   aCCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATAc                                         <  2:1424306/101‑1 (MQ=255)                                                    ccAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATAcc                                        <  2:1429006/101‑1 (MQ=255)                                                      aaCGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGt                                      >  2:1664004/1‑101 (MQ=255)                                                      aaCGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGt                                      >  1:1150784/1‑101 (MQ=255)                                                      aaCGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGt                                      >  1:343930/1‑101 (MQ=255)                                                      aaCGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGt                                      >  2:1556099/1‑101 (MQ=255)                                                       aCGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTg                                     <  1:1341981/101‑1 (MQ=255)                                                        cGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGa                                    >  2:331449/1‑101 (MQ=255)                                                            gACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCa                                >  2:31825/1‑101 (MQ=255)                                                               ggAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATaa                             <  2:80200/101‑1 (MQ=255)                                                               ggAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATaa                             >  2:1262489/1‑101 (MQ=255)                                                                gAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATaan                            <  1:639283/101‑2 (MQ=255)                                                                 aaaaGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATc                           <  2:1672715/101‑1 (MQ=255)                                                                  aaaGCGGATTTAACCGGTTGTAACCCGAGCTGGGGCTGCGAAGGTGCAATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCa                          <  2:309500/101‑1 (MQ=255)                                                                       ggATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGa                     <  1:1388745/101‑1 (MQ=255)                                                                         aTTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAAc                   >  2:1380735/1‑101 (MQ=255)                                                                             aaCCGGTAGTAACCCTAGCTGGGGATGCGAAGGGGCGATCAACCACCAGCTTGCCGCGTATGAAATGACCCATACAGAGACGCATAATCAGGGGAAATAcg               >  2:741941/1‑101 (MQ=255)                                                                               ccGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTAcgcg             >  2:788853/1‑101 (MQ=255)                                                                                     gTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTACGCGGCgatg       <  1:1678232/101‑1 (MQ=255)                                                                                     gTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTACGCGGCgatg       >  1:1289063/1‑101 (MQ=255)                                                                                       aaCCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTACGCGGCgatgat     <  1:112946/101‑1 (MQ=255)                                                                                       aaCCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTACGCGGCgatgat     <  1:1698234/101‑1 (MQ=255)                                                                                        aCCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTACGCGGCGatgatg    >  1:184024/1‑101 (MQ=255)                                                                                        aCCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTACGCGGCGatgatg    >  2:153551/1‑101 (MQ=255)                                                                                         cccTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTACGCGGCGATGATgg   >  2:785187/1‑101 (MQ=255)                                                                                         cccTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTACGCGGCGATGATgg   <  1:893873/101‑1 (MQ=255)                                                                                          ccTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTACGCGGCGATGATGGc  >  1:1030309/1‑101 (MQ=255)                                                                                             |                                                                                                  TCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTGGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTACGCGGCGATGATGGC  >  118/13445‑13634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑