Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	191	1,822	C→T	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	191	1,822	0	C	T	96.5%	59.2 / ‑3.7	29	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (10/18);  total (10/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01

AGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCGCTCTCGCTTTCGGCTGCTGCATTCTGGCTATTTTTCGCCGCAGTTTCACTGGCTTTGGCATTCGTTTCGCTGGTTTTCGCTGCCGTCTGGCTGGACTTTGCGTTGGTTTCGCTCGTCTTTGCTGCTGTCTCGCTATTTTTCGCGTTGTTT  >  191/1724‑1919                                                                                                   |                                                                                                  agcagTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGttt                                                                                                 <  1:1357832/101‑1 (MQ=17)    agTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGGACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTgg                                                                                              >  2:569838/1‑101 (MQ=17)       tGCTGATCCAGCTGCTGAAGTGGCAGACCCGGCTGCCGCACTCTCGCTTTCGGCTTCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCAt                                                                                           <  2:163311/101‑1 (MQ=14)       tGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCAt                                                                                           <  2:485577/101‑1 (MQ=14)        gCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCAtt                                                                                          <  2:1362197/101‑1 (MQ=14)         cTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCATTc                                                                                         >  1:567270/1‑101 (MQ=17)         cTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCATTc                                                                                         >  2:716364/1‑101 (MQ=14)         cTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCATTc                                                                                         <  1:107575/101‑1 (MQ=14)          tGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCATTCg                                                                                        >  1:360556/1‑101 (MQ=17)           gATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCATTCGt                                                                                       <  1:320482/101‑1 (MQ=14)            aTCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCATTCGtt                                                                                      <  2:1031517/101‑1 (MQ=14)             tCCAGCTGCTGAAGTCGCAGAACCGGCTGCTGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCATTCGttt                                                                                     <  1:638281/101‑1 (MQ=14)               cAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCATTCGTTTCg                                                                                   <  2:1544540/101‑1 (MQ=14)               cAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCATTCGTTTCg                                                                                   <  1:1299320/101‑1 (MQ=14)                   tgctgAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCATTCGTTTCGCttg                                                                               >  1:1089836/1‑99 (MQ=14)                     ctgAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTTCTGCAGCCTGACTGCTTTTCGCCGCCGTTTCACTGGTTTTGGCATTCGTTTCGCTTGtt                                                                             >  1:787250/1‑101 (MQ=11)                     ctgAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCATTCGTTTCGCTTGtt                                                                             >  1:447397/1‑101 (MQ=11)                      tgAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGTTTCACTGGTTTTGGCATTCGTTTCGCTTGttt                                                                            <  2:343351/101‑1 (MQ=11)                                                                    gcctgactgcttttCGCCGCAGTTTCACTGGTTTTGGCATTCGTTTCGCTTGTTTTCGCTGCCGCCTGGCTGGACTTTGCGTTGGTTTCGCTCGTCTTtgc                              <  2:1392122/91‑1 (MQ=11)                                                                     cctgactgcttttCGCCGCAGTTTCACTGGTTTTGGCATTCGTTTCGCTTGTTTTCGCTGCCGCCTGGCTGGACTTTGCGTTGGTTTCGCTCGTCTTtgct                             >  2:500675/10‑101 (MQ=11)                                                                              ttttCGCCGCAGTTTCACTGGTTTTGGCATTCGTTTCGCTTGTTTTCGCTGCCGCCTGGCTGGACTTTGCGTTGGTTTCGCTCGTCTTTGCTGCTGTCTCg                    <  2:1613460/101‑1 (MQ=255)                                                                               tttCGCCGCAGTTTCACTGGTTTTGGCATTCGTTTCGCTTGTTTTCGCTGCCGCCTGGCTGGACTTTGCGTTGGTTTCGCTCGTCTTTGCTGCTGTCTCGc                   >  2:1430797/1‑101 (MQ=255)                                                                                ttCGCCGCAGTTTCACTGGTTTTGGCATTCGTTTCGCTTGTTTTCGCTGCCGCCTGGCTGGACTTTGCGTTGGTTTCGCTCGTCTTTGCTGCTGTCTCGCt                  <  2:567265/101‑1 (MQ=255)                                                                                ttCGCCGCAGTTTCACTGGTTTTGGCATTCGTTTCGCTTGTTTTCGATGCCGCCTGGCTGGACTTTGCGTTGGTTTCGCTCGTCTTTGCTGCTGTCTCGCt                  >  2:314650/1‑101 (MQ=17)                                                                                  cgccgcAGTTTCACTGGTTTTGGCATTCGTTTCGCTTGTTTTCGCTGCCGCCTGGCTGGACTTTGCGTTGGTTTCGCTCGTCTTTGCTGCTGTCTCGCTAt                <  1:913249/101‑1 (MQ=255)                                                                                   gccgcAGTTTCACTGGTTTTGGCATTCGTTTCGCTTGTTTTCGCTGCCGCCTGGCTGGACTTTGCGTTGGTTTCGCTCGTCTTTGCTGCTGTCTCGCTAtt               <  2:792654/101‑1 (MQ=255)                                                                                   gccgcAGTTTCACTGGCTTTGGCATTCGTTTCGCTGGTTTTCGCTGCCGTCTGGCTGGACTTTGCGTTGGTTTCGCTCGTCTTTGCGGCTGTCTCGCTGtt               <  2:699982/101‑1 (MQ=255)                                                                                      gcAGTTTCACTGGTTTTGGCATTCGTTTCGCTTGTTTTCGCTGCCGCCTGGCTGGACTTTGCGTTGGTTTCGCTCGTCTTTGCTGCTGTCTCGCTAttttt            <  2:373090/101‑1 (MQ=255)                                                                                           ttCACTGGTTTTGGCATTCGTTTCGCTTGTTTTCGCTGCCGCCTGGCTGGACTTTGCGTTGGTTTCGCTCGTCTTTGCTGCTGTCTCGCTATTTTTCGCgt       <  1:1181684/101‑1 (MQ=255)                                                                                            tCACTGGTTTTGGCATTCGTTTCGCTTGTTTTCGCTGCCGCCTGGCTGGACTTTGCGTTGGTTTCGCTCGTCTTTGCTGCTGTCTCGCTATTTTTCGCgtt      >  1:444403/1‑101 (MQ=21)                                                                                                tGGTTTTGGCATTCGTTTCGCTTGTTTTCGCTGCCGCCTGGCTGGACTTTGCGTTGGTTTCGCTCGTCTTTGCTGCTGTCTCGCTATTTTTCGCGTTGttt  <  2:447392/101‑1 (MQ=255)                                                                                                   |                                                                                                  AGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCGCTCTCGCTTTCGGCTGCTGCATTCTGGCTATTTTTCGCCGCAGTTTCACTGGCTTTGGCATTCGTTTCGCTGGTTTTCGCTGCCGTCTGGCTGGACTTTGCGTTGGTTTCGCTCGTCTTTGCTGCTGTCTCGCTATTTTTCGCGTTGTTT  >  191/1724‑1919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑