Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	136	19	+T	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	136	19	1	.	T	100.0%	236.2 / NA	67	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (27/40);  total (27/40)

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTTTGGGGTCAGTACATTT‑CAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCG  >  136/1‑116                                                                                            |                                                                                                  tgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCttt                                                                                          <  2:775918‑M1/29‑1 (MQ=255) tgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCttt                                                                                          <  2:1150422‑M1/29‑1 (MQ=255) tgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCttt                                                                                          >  1:872176‑M1/73‑101 (MQ=255)  gaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCtttt                                                                                         >  2:1321153‑M1/72‑101 (MQ=255)   aaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCttttt                                                                                        >  2:54763‑M1/71‑101 (MQ=255)   aaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCttttt                                                                                        >  1:1486536‑M1/71‑101 (MQ=255)   aaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCttttt                                                                                        >  2:45971‑M1/71‑101 (MQ=255)     attaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCa                                                                                      <  1:534251‑M1/33‑1 (MQ=255)         aaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAg                                                                                  >  1:1021004‑M1/65‑101 (MQ=255)         aaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAg                                                                                  <  2:473770‑M1/37‑1 (MQ=255)           aggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGcc                                                                                <  1:386256‑M1/39‑1 (MQ=255)           aggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGcc                                                                                >  1:514019‑M1/63‑101 (MQ=255)                 gactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCt                                                                          <  2:754383‑M1/45‑1 (MQ=255)                      caattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATc                                                                     <  2:495947‑M1/50‑1 (MQ=255)                      caattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATc                                                                     <  2:798676‑M1/50‑1 (MQ=255)                       aattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATcg                                                                    <  1:1227492‑M1/51‑1 (MQ=255)                       aattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATcg                                                                    <  1:1235210‑M1/51‑1 (MQ=255)                        attgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATcgc                                                                   <  1:206869‑M1/52‑1 (MQ=255)                          tgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGt                                                                 <  1:798510‑M1/54‑1 (MQ=255)                           gaatatcggaatcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGtt                                                                <  1:1229718‑M1/55‑1 (MQ=255)                            aatatcggaatcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGttt                                                               >  1:150360‑M1/46‑101 (MQ=255)                              tatcggaatcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTaa                                                             <  1:1126712‑M1/58‑1 (MQ=255)                                  ggaatcagtcctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCAc                                                         >  1:495952‑M1/40‑101 (MQ=255)                                   gaatcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACt                                                        >  1:183604‑M1/39‑101 (MQ=255)                                    aatcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACtt                                                       <  1:1560540‑M1/64‑1 (MQ=255)                                     atcagacctatatggctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACttt                                                      >  2:1143716‑M1/37‑101 (MQ=255)                                     atcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACttt                                                      <  2:68144‑M1/65‑1 (MQ=255)                                     atcagacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACttt                                                      <  2:532109‑M1/65‑1 (MQ=255)                                        agacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCAc                                                   <  1:1219144‑M1/68‑1 (MQ=255)                                         gacctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCAcc                                                  >  1:1246411‑M1/33‑101 (MQ=255)                                          acctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCt                                                 <  2:1273793‑M1/70‑1 (MQ=255)                                           cctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTg                                                <  1:164424‑M1/71‑1 (MQ=255)                                            ctatatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTgc                                               <  2:532788‑M1/72‑1 (MQ=255)                                               tatgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGcc                                            >  1:775923‑M1/27‑101 (MQ=255)                                                atgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCa                                           <  2:367352‑M1/76‑1 (MQ=255)                                                 tgcctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAg                                          >  2:248679‑M1/25‑101 (MQ=255)                                                   cctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAggg                                        <  1:1658054‑M1/79‑1 (MQ=255)                                                    ctcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGt                                       <  2:910633‑M1/80‑1 (MQ=255)                                                     tcgtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGccaggatc                                      >  2:1071544‑M1/21‑98 (MQ=255)                                                       gtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAg                                    <  2:944486‑M1/83‑1 (MQ=255)                                                       gtgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAg                                    <  1:595948‑M1/83‑1 (MQ=255)                                                        tgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAgg                                   >  2:265284‑M1/18‑101 (MQ=255)                                                        tgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAgg                                   <  2:1210726‑M1/84‑1 (MQ=255)                                                        tgtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAgg                                   <  1:106661‑M1/84‑1 (MQ=255)                                                         gtttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGt                                  <  1:146578‑M1/85‑1 (MQ=255)                                                          tttaactgtccaactTTTTGGGGTCAGTACATTTTCAGGTCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGtt                                 >  2:206923‑M1/16‑101 (MQ=255)                                                             aactgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTc                              <  1:736755‑M1/89‑1 (MQ=255)                                                              actgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCg                             >  1:798681‑M1/12‑101 (MQ=255)                                                                tgtccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGAt                           <  2:523231‑M1/92‑1 (MQ=255)                                                                tgtccaactTTTTGGGGGCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGAt                           <  2:1428502‑M1/92‑1 (MQ=255)                                                                  tccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATaa                         <  2:1503475‑M1/94‑1 (MQ=255)                                                                  tccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATaa                         >  2:456252‑M1/8‑101 (MQ=255)                                                                  tccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATaa                         >  2:1210062‑M1/8‑101 (MQ=255)                                                                   ccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAAt                        <  1:1257312‑M1/95‑1 (MQ=255)                                                                   ccaactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAAt                        >  2:274111‑M1/7‑101 (MQ=255)                                                                     aactTTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCg                      >  1:331095‑M1/5‑101 (MQ=255)                                                                         TTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAg                  >  2:1265962‑M1/1‑101 (MQ=255)                                                                         TTTTGGGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAg                  >  1:532793‑M1/1‑101 (MQ=255)                                                                              GGGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAg             >  1:532114‑M1/1‑101 (MQ=255)                                                                               GGTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAgg            <  1:418779‑M1/101‑1 (MQ=255)                                                                                GTCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAggg           <  1:1144591‑M1/101‑1 (MQ=255)                                                                                 TCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGa          <  2:898719‑M1/101‑1 (MQ=255)                                                                                 TCAGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGa          <  1:1543606‑M1/101‑1 (MQ=255)                                                                                   gttcatttt‑CAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACa       <  2:1444095/95‑1 (MQ=255)                                                                                   AGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAAc        <  1:347107‑M1/101‑1 (MQ=255)                                                                                   AGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAAc        <  2:1637490‑M1/101‑1 (MQ=255)                                                                                   AGTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAAc        >  2:1126707‑M1/1‑101 (MQ=255)                                                                                    tacatttt‑CAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAg      <  2:164424/96‑1 (MQ=255)                                                                                    GTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACa       >  1:1579741‑M1/1‑101 (MQ=255)                                                                                    GTACATTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACa       >  1:569397‑M1/1‑101 (MQ=255)                                                                                       atttt‑CAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTc   >  2:1250192/3‑101 (MQ=255)                                                                                       atttt‑CAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTc   >  2:935674/3‑101 (MQ=255)                                                                                       atttt‑CAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAACCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTc   >  2:260394/3‑101 (MQ=255)                                                                                        tttt‑CAGGTTTTGTTCAGGAGCCGGCGATTTACCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGTGAACAGCTCg  <  2:1131293/100‑1 (MQ=255)                                                                                            |                                                                                                  ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTTTGGGGTCAGTACATTT‑CAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCG  >  136/1‑116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑