Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	191	1,969	A→C	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	191	1,969	0	A	C	100.0%	165.4 / NA	47	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (25/22);  total (25/22)

TGGTTTCGCTCGTCTTTGCTGCTGTCTCGCTATTTTTCGCGTTGTTTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGACGCTGCCGCCTGTGCGCTGTTAGCTGCATTCGTTTCTGAGGTTTTCGCCGCGTTCTTCGATGATGCCGCTGCTGTTTCTGATTTCTTTGCTGCCGC  >  191/1873‑2065                                                                                                 |                                                                                                 tGGTTTCGCTCGTCTTTGCTGCTGTCTCGCTATTTTTCGCGTTGTTTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCt                                                                                              <  2:568862/101‑1 (MQ=255)   gTTTCGCTCGTCTTTGCTGCTGTCTCGCTATTTTTCGCGTTGTTTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTgc                                                                                            >  1:882246/1‑101 (MQ=255)        gCTCGTCTTTGCTGCTGTCTCGCTATTTTTCGCGTTGTTTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCt                                                                                       <  2:1268281/101‑1 (MQ=255)         cTCGTCTTTGCTGCTGTCTCGCTATTTTTCGCGTTGTTTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCtg                                                                                      >  1:1050995/1‑101 (MQ=255)          tCGTCTTTGCTGCTGTCTCGCTATTTTTCGCGTTGTTTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCtgt                                                                                     <  2:800293/101‑1 (MQ=255)            gTCTTTGCTGCTGTCTCGCTATTTTTCGCGTTGTTTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTgc                                                                                   >  1:994554/1‑101 (MQ=255)             tCTTTGCTGCTGTCTCGCTATTTTTCCCGTTGTTTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTgcg                                                                                  >  1:1718515/1‑101 (MQ=255)              cTTTGCTGCTGTCTCGCTATTTTTCGCGTTGTTTTCTGATTTTTTAGCTGCTGTCGCGGCGTTTGCAGCTGCAGTCTGCGAGGCCACTGCCGCCTGTgcgt                                                                                 >  2:1528967/1‑100 (MQ=255)                 tgctgcTGTCTCGCTATTTTTCGCGTTGTTTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGt                                                                              <  1:71354/101‑1 (MQ=255)                   ctgctgTCTCGCTATTTTTCGCGTTGTTTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTa                                                                            >  1:1442863/1‑101 (MQ=255)                   ctgctgTCTCGCTATTTTTCGCGTTGTTTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTa                                                                            >  1:299366/1‑101 (MQ=255)                    tgctgTCTCGCTATTTTTCGCGTTGTTTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAg                                                                           >  1:1184228/1‑101 (MQ=255)                      ctgTCTCGCTATTTTTCGCGTTGTTTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCt                                                                         >  2:961695/1‑101 (MQ=255)                      ctgTCTCGCTATTTTTCGCGTTGTTTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCt                                                                         <  1:716369/101‑1 (MQ=255)                          ctcGCTATTTTTCGCGTTGTTTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCAt                                                                     >  1:1086325/1‑101 (MQ=255)                          ctcGCTATTTTTCGCGTTGTTTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCAt                                                                     <  1:807601/101‑1 (MQ=255)                              cTATTTTTCGCGTTGTTTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGt                                                                 >  1:1206536/1‑101 (MQ=255)                                  ttttCGCGTTGTTTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCt                                                             <  1:644135/101‑1 (MQ=255)                                      cgcgTTGTTTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAgg                                                         <  2:681480/101‑1 (MQ=255)                                        cgTTGTTTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGtt                                                       >  1:477888/1‑101 (MQ=255)                                        cgTTGTTTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGtt                                                       <  1:1624046/101‑1 (MQ=255)                                        cgTTGTTTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGtt                                                       >  2:322734/1‑101 (MQ=255)                                          ttgttTTCTGATGTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGtttt                                                     <  2:170456/101‑1 (MQ=255)                                           tgttTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTc                                                    >  2:1134979/1‑101 (MQ=255)                                           tgttTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTc                                                    >  2:743071/1‑101 (MQ=255)                                           tgttTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTc                                                    <  2:1313388/101‑1 (MQ=255)                                            gttTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTcg                                                   >  1:46238/1‑101 (MQ=255)                                            gttTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTcg                                                   <  2:360556/101‑1 (MQ=255)                                              tttCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTcgcc                                                 >  1:60886/1‑101 (MQ=255)                                              tttCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTcgcc                                                 <  1:961700/101‑1 (MQ=255)                                                   gATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTCGCCGCGtt                                            <  2:982840/101‑1 (MQ=255)                                                    aTTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTCGCCGCGttc                                           >  1:612245/1‑101 (MQ=255)                                                          tAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTCGCCGCGTTCTTCgat                                     <  2:787245/101‑1 (MQ=255)                                                          tAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTCGCCGCGTTCTTCgat                                     <  2:1050990/101‑1 (MQ=255)                                                          tAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTCGCCGCGTTCTTCgat                                     <  1:364344/101‑1 (MQ=255)                                                           aGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTCGCCGCGTTCTTCgatg                                    <  1:520903/101‑1 (MQ=255)                                                             ctgctgTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTCGCCGCGTTCTTCgatgat                                  <  1:1090231/101‑1 (MQ=255)                                                              tgctgTCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTCGCCGCGTTCTTCGatgatg                                 >  1:1278797/1‑101 (MQ=255)                                                                   tCGCGGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTCGCTGCGTTCTTCGATGATGCCgct                            <  1:55742/101‑1 (MQ=255)                                                                      cgGAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTCGCCGCGTTCTTCGATGATGCCgctgct                         <  1:616430/101‑1 (MQ=255)                                                                       ggAGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTCGCCGCGTTCTTCGATGATGCCGctgctg                        >  2:1366930/1‑101 (MQ=255)                                                                         aGTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTCGCCGCGTTCTTCGATGATGCCGCTGCTGtt                      <  2:1278792/101‑1 (MQ=255)                                                                          gTTTGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTCGCCGCGTTCTTCGATGATGCCGCTGCTGttt                     <  1:1430803/101‑1 (MQ=255)                                                                           tttGCCGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTCGCCGCGTTCTTCGATGATGCCGCTGCTGTTTc                    >  2:1488463/1‑101 (MQ=255)                                                                                cGATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTCGCCGCGTTCTTCGATGATGCCGCTGCTGTTTCTGCtt               >  2:1492651/1‑101 (MQ=255)                                                                                 gATGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTCGCCGCGTTCTTCGATGATGCCGCTGCTGTTTCTGAttt              >  2:115120/1‑101 (MQ=255)                                                                                   tGCAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTCGCCGCGTTCTTCGATGATGCCGCTGCTGTTTCTGATctct            >  2:1474754/1‑101 (MQ=255)                                                                                     cAGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTCGCCGCGTTCTTCGATGATGCCGCTGCTGTTTCTGATTTCttt          <  1:743076/101‑1 (MQ=255)                                                                                      aGTCTGCGAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTCGCCGCGTTCTTCGATGATGCCGCTGCTGTTTCTGATTTCTTtg         >  2:556277/1‑101 (MQ=255)                                                                                             gAGGCCGCTGCCGCCTGTGCGCTGTTAGCTGCATGCGTTTCTGAGGTTTTCGCCGCGTTCTTCGATGATGCCGCTGCTGTGTCTGATTTCTTTGCTGCCGc  >  1:580105/1‑101 (MQ=255)                                                                                                 |                                                                                                 TGGTTTCGCTCGTCTTTGCTGCTGTCTCGCTATTTTTCGCGTTGTTTTCTGATTTTTTAGCTGCTGTCGCGGAGTTTGCCGATGCAGTCTGCGAGGACGCTGCCGCCTGTGCGCTGTTAGCTGCATTCGTTTCTGAGGTTTTCGCCGCGTTCTTCGATGATGCCGCTGCTGTTTCTGATTTCTTTGCTGCCGC  >  191/1873‑2065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑