Predicted mutation
evidence seq id position mutation annotation gene description
RA 19 2,623 G→T intergenic (–/–)  /  –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*192,6230GT97.1% 232.4 / ‑0.9 68intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (30/36);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-02

GACGTGCTGCATGAACCGGGCGGCTGTCTAAAATCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCGAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCGAGAAAATGCCAAAGCGCAGGCAGCTCCCGTGGG  >  19/2526‑2713
                                                                                                 |                                                                                          
gACGTGCTGCATGAACCGGGCGGCTGTCTAAAATCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTaaa                                                                                         <  2:320147/101‑1 (MQ=255)
 aCGTGCTGCATGAACCGGGCGGCTGTCTAAAATCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTaaaa                                                                                        >  1:435177/1‑101 (MQ=255)
   gTGCTGCATGAACCGGGCGGCTGTCTAAAATCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAAc                                                                                      >  1:981819/1‑101 (MQ=255)
   gTGCTGCATGAACCGGGCGGCTGTCTAAAATCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAAc                                                                                      <  2:1445105/101‑1 (MQ=255)
       tgcATGAACCGGGCGGCTGTCTAAAATCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACtgtg                                                                                  >  1:1278378/1‑101 (MQ=255)
          aTGAACCGGGCGGCTGTCTAAAATCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGAt                                                                               <  2:229975/101‑1 (MQ=255)
          aTGAACCGGGCGGCTGTCTAAAATCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGAt                                                                               <  1:1196118/101‑1 (MQ=255)
          aTGAACCGGGCGGCTGTCTAAAATCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGAt                                                                               >  2:1323347/1‑101 (MQ=255)
          aTGAACCGGGCGGCTGTCTAAAATCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGAt                                                                               >  1:639765/1‑101 (MQ=255)
          aTGAACCGGGCGGCTGTCTAAAATCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGAt                                                                               <  2:1355594/101‑1 (MQ=255)
           tGAACCGGGCGGCTGTCTAAAATCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATg                                                                              <  2:861562/101‑1 (MQ=255)
            gAACCGGGCGGCTGTCTAAAATCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATgg                                                                             >  2:1016296/1‑101 (MQ=255)
             aaCCGGGCGGCTGTCTAAAATCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGc                                                                            <  2:32715/101‑1 (MQ=255)
                cGGGCGGCTGTCTAAAATCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGa                                                                         <  2:742750/101‑1 (MQ=255)
                   gcggcTGTCTAAAATCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCGAAAAACTGTGGATGGCGGACtt                                                                      <  1:584744/101‑1 (MQ=255)
                    cggcTGTCTAAAATCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTc                                                                     >  1:1147366/1‑101 (MQ=255)
                     ggcTGTCTAAAATCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGGCtttc                                                                    >  2:1660011/1‑99 (MQ=255)
                     ggcTGTCTAAAATCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTAcc                                                                    <  1:280037/101‑1 (MQ=255)
                       gtGTCTAAAATCACCCGATCAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCAc                                                                  <  2:445773/100‑1 (MQ=255)
                       cTGTCTAAAATCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCAc                                                                  <  1:1222821/101‑1 (MQ=255)
                       cTGTCTAAAATCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCAc                                                                  <  2:589709/101‑1 (MQ=255)
                        tGTCTAAAATCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACg                                                                 >  1:844515/1‑101 (MQ=255)
                         gTCTAAAATCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGc                                                                >  1:1487879/1‑101 (MQ=255)
                             aaaaTCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATa                                                            <  1:1709410/101‑1 (MQ=255)
                              aaaTCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATaa                                                           <  2:521050/101‑1 (MQ=255)
                                tcttccgatctAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAAtt                                                         <  2:498736/91‑1 (MQ=255)
                                    cccGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAAt                                                     <  1:291807/101‑1 (MQ=255)
                                      cGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTc                                                   >  2:503771/1‑101 (MQ=255)
                                      cGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTc                                                   <  2:1364903/101‑1 (MQ=255)
                                       gATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCCTAATTAAATTCg                                                  >  1:39671/1‑101 (MQ=255)
                                          tAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCa                                               <  1:1016301/101‑1 (MQ=255)
                                           aaCGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAGAtcggaaga                                              >  1:498741/1‑94 (MQ=255)
                                             cgccgcGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGa                                            >  1:1451652/1‑101 (MQ=255)
                                                cgcgCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAACCTGTGGCTGGCAGCCTTCCACGCCATAATTAAATTCGCCAGGgaga                                         >  2:585659/1‑101 (MQ=255)
                                                 gcgcTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGCCTTCCACGCCATAATTAAATTCGCCAGGAagag                                        <  1:1445532/101‑1 (MQ=255)
                                                  cgcTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAgaga                                       <  1:151015/101‑1 (MQ=255)
                                                    cTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAAt                                     >  2:855354/1‑101 (MQ=255)
                                                    cTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAAt                                     >  2:1159287/1‑101 (MQ=255)
                                                     tGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATc                                    <  1:1560809/101‑1 (MQ=255)
                                                     tGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATc                                    <  2:1487873/101‑1 (MQ=255)
                                                     tGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATc                                    <  2:981814/101‑1 (MQ=255)
                                                      gATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCg                                   >  1:1240288/1‑101 (MQ=255)
                                                       aTGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCga                                  >  1:941108/1‑101 (MQ=255)
                                                       aTGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCga                                  <  2:397843/101‑1 (MQ=255)
                                                       aTGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCga                                  >  2:889103/1‑101 (MQ=255)
                                                        tGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCgag                                 <  1:528383/101‑1 (MQ=255)
                                                         gTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCgaga                                >  1:207754/1‑101 (MQ=255)
                                                          tAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCGAGaa                               >  1:176525/1‑101 (MQ=255)
                                                          tAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCGAGaa                               <  2:252946/101‑1 (MQ=255)
                                                             aCCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCGAGAAAAt                            <  2:990047/101‑1 (MQ=255)
                                                             aCCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCGAGAAAAt                            <  2:505121/101‑1 (MQ=255)
                                                                 cgATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCGAGAAAATGCCa                        <  2:418289/101‑1 (MQ=255)
                                                                  gATTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCGAGAAAATGCCaa                       <  2:1209219/101‑1 (MQ=255)
                                                                   aTTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCGAGAAAATGCCaaa                      <  2:835952/101‑1 (MQ=255)
                                                                   aTTAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCGAGAAAATGCCaaa                      >  1:612272/1‑101 (MQ=255)
                                                                    ttAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCGAGAAAATGCCAAAg                     <  1:541857/101‑1 (MQ=255)
                                                                     tAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGGGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCGAGAAAATGCCAAAgc                    >  2:28146/1‑101 (MQ=255)
                                                                     tAAGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCGAGAAAATGCCAAAgc                    >  2:1569673/1‑101 (MQ=255)
                                                                      aaGCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCGAGAAAATGCCAAAgcg                   >  1:384102/1‑101 (MQ=255)
                                                                        gCGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCGAGAAAATGCCAAAGCgca                 <  1:1228478/101‑1 (MQ=255)
                                                                         cGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCGAGAAAATGCCAAAGCgcag                <  2:435172/101‑1 (MQ=255)
                                                                         cGTTTGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCACCAGGAAGAAAATCGAGAAAATGCCAAAGCgcag                >  1:91210/1‑101 (MQ=255)
                                                                           tttGTTCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCGAGAAAATGCCAAAGCgcaggc              >  2:310385/1‑101 (MQ=255)
                                                                           tttGTTCCTCTTCTCCTTTGGCGAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCGAGAAAATGCCAAAGCgcaggc              >  2:622664/1‑101 (MQ=255)
                                                                                tCCTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCGAGAAAATGCCAAAGCGCAGGCAGCTc         >  1:258945/1‑101 (MQ=255)
                                                                                 ccTCTTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCGAGAAAATGCCAAAGCGCAGGCAGCTcc        <  2:1483406/101‑1 (MQ=255)
                                                                                  ctctTCTCCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCGAGAAAATGCCAAAGCGCAGGCAGCTccc       <  2:1224493/101‑1 (MQ=255)
                                                                                      tctcCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCGAGAAAATGCCAAAGCGCAGGCAGCTCCCGTgg   <  1:420246/101‑1 (MQ=255)
                                                                                       ctcCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCGAGAAAATGCCAAAGCGCAGGCAGCTCCCGTggg  >  2:939137/1‑101 (MQ=255)
                                                                                       ctcCTTTGGCTAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCGAGAAAATGCCAAAGCGCAGGCAGCTCCCGTggg  >  1:1321206/1‑101 (MQ=255)
                                                                                                 |                                                                                          
GACGTGCTGCATGAACCGGGCGGCTGTCTAAAATCACCCGATTAACGCCGCGCTGATGTAAACCGCGATTAAGCGTTTGTTCCTCTTCTCCTTTGGCGAAAAACTGTGGATGGCGGACTTCCACGCCATAATTAAATTCGCCAGGAAGAGAATCGAGAAAATGCCAAAGCGCAGGCAGCTCCCGTGGG  >  19/2526‑2713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: