Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	168	8,132	A→G	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	168	8,132	0	A	G	100.0%	51.8 / NA	17	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/4);  total (13/4)

GGATGAGTTTAGGTTGTGGTGAGTGGGGGTTGTGTTTAAGGACGGGGAGAGTCGGGGTATTACTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCG‑‑‑‑‑‑‑‑  >  168/8034‑8158                                                                                                   |                                   ggATGAGTTTAGGTTGTGGTGAGTGGGGGTTGTGTTTAAGGACGGGGAGAGTCGGGGTATTACTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGGTa                                  >  1:828572/1‑101 (MQ=255) ggATGAGTTTAGGTTGTGGTGAGTGGGGGTTGTGTTTAAGGACGGGGAGAGTCGGGGTATTACTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGGTa                                  >  2:159634/1‑101 (MQ=255) ggATGAGTTTAGGTTGTGGCGAGTGGGGGTTGTGTTTAAGGACGGGGAGAGTCGGGGTGTTACTACGAAAGCCCGCTCCCCGCAAGGACTGAGGCCAGGTa                                  >  2:801721/1‑101 (MQ=38)   aTGAGTTTAGGTTGTGGTGAGTGGGGGTTGTGTTTAAGGACGGGGAGAGTCGGGGTATTACTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGGTAGt                                >  1:1690959/1‑101 (MQ=255)     gAGTTTAGGTTGTGGTGAGTGGGGGTTGTGTTTAAGGACGGGGAGAGTCGGGGTATTACTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGGTAGttt                              >  2:237173/1‑101 (MQ=255)        tttAGGTTGTGGTGAGTGGGGGTTGTGTTTAAGGACGGGGAGAGTCGGGGTATTACTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGGTAGTTTCTg                           >  1:1073870/1‑101 (MQ=255)           aGGTTGTGGTGAGTGGGGGTTGTGTTTAAGGACGGGGAGAGTCGGGGTATTACTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGGTAGTTTCTGTcc                        >  2:1098015/1‑101 (MQ=255)               tgtgGTGAGTGGGGGTTGTGTTTAAGGACGGGGAGAGTCGGGGTATTACTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGGTAGTTTCTGTCCATgg                    >  2:1510609/1‑101 (MQ=255)                      aGTGGGGGTTGTGTTTAAGGACGGGGAGAGTCGGGGTATTACTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGGTAGTTTCTGTCCATGGCTGCttt             >  1:1557009/1‑101 (MQ=255)                       gTGGGGGTTGTGTTTAAGGACGGGGAGAGTCGGGGTATTACTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGGTAGTTTCTGTCCATGGCTGCtttt            >  2:945674/1‑101 (MQ=255)                         gggggTTGTGTTTAAGGACGGGGAGAGTCGGGGTATTACTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGGTAGTTTCTGTCCATGGCTGCTTTTCg          <  2:1690953/101‑1 (MQ=255)                           gggTTGTGTTTAAGGACGGGGAGAGTCGGGGTATTACTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGGTAGTTTCTGTCCATGGCTGCTTTTCgca        <  1:233760/101‑3 (MQ=255)                             gTTGTGTTTAAGGACGGGGAGAGTCGGGGTATTACTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGGTAGTTTCTGTCCATGGCTGCTTTTCgcatc      >  1:345736/1‑97 (MQ=255)                             gTTGTGTTTAAGGACGGGGAGAGTCGGGGTATTACTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGGTAGTTTCTGTCCATGGCTGCTTTTCgcatc      >  1:1689481/1‑97 (MQ=255)                             gTTGTGTTTAAGGACGGGGAGAGTCGGGGTATTACTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGGTAGTTTCTGTCCATGGCTGCTTTTCgcatc      >  1:1290909/1‑97 (MQ=255)                              ttGTGTTTAAGGACGGGGAGAGTCGGGGTATTACTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGGTAGTTTCTGTCCATGGCTGCTTTTCgcatct     <  2:1360480/101‑6 (MQ=255)                                 tgtTTAAGGACGGGGAGAGTCGGGGTATTACTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGGTAGTTTCTGTCCATGGCTGCTTTTCgcatcttac  <  2:616478/101‑9 (MQ=18)                                 tgtTTAAGGACGGGGAGAGTCGGGGTATTACTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGGTAGTTTCTGTCCATGGCTGCTTTTCgcatcttac  >  2:333930/1‑93 (MQ=18)                                                                                                   |                                   GGATGAGTTTAGGTTGTGGTGAGTGGGGGTTGTGTTTAAGGACGGGGAGAGTCGGGGTATTACTACGAAAGCCCGCTCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCG‑‑‑‑‑‑‑‑  >  168/8034‑8158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑