Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	57	2,274	C→T	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	57	2,274	0	C	T	100.0%	193.3 / NA	56	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/28);  total (28/28)

AACCCTTTGACGTGTTTTTCACCTACCAGCGGCACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGACTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTAAGCCAGCCCCAACTCTTTCGCGCCTGTCGAGTTCGGTTCTAAG  >  57/2177‑2371                                                                                                  |                                                                                                  aacCCTTTGACGTGTTTTTCACCTACCAGCGGCACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGa                                                                                                >  2:1481887/1‑101 (MQ=255) aacCCTTTGACGTGTTTTTCACCTACCAGCGGCACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGa                                                                                                >  1:1430603/1‑101 (MQ=255)  acCCTTTGACGTGTTTTTCACCTACCAGCGGCACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGaa                                                                                               <  1:529443/101‑1 (MQ=255)     cTTTGACGTGTTTTTCACCTACCAGCGGCACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAgg                                                                                            <  2:453357/101‑1 (MQ=255)     cTTTGACGTGTTTTTCACCTACCAGCGGCACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTAGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAgg                                                                                            <  1:1363226/101‑1 (MQ=255)       ttGACGTGTTTTTCACCTACCAGCGGCACCAGTGTCGAGCTGGGGCGAATCGCTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTAAAAGGaa                                                                                          <  2:303546/101‑1 (MQ=255)       ttGACGTGTTTTTCACCTACCAGCGGCACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGaa                                                                                          <  2:101486/101‑1 (MQ=255)       ttGACGTGTTTTTCACCTACCAGCGGCACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGaa                                                                                          <  1:908855/101‑1 (MQ=255)          aCGTGTTTTTCACCTACCAGCGGCACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTNAGNGAAGGNGATTGAAAGGAAgat                                                                                       >  2:1026521/1‑99 (MQ=255)          aCGTGTTTTTCACCTACCAGCGGCACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCt                                                                                       >  1:1135701/1‑101 (MQ=255)          aCGTGTTTTTCACCTACCAGCGGCACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCt                                                                                       <  1:299077/101‑1 (MQ=255)           cGTGTTTTTCACCTACCAGCGGCACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTg                                                                                      >  2:670205/1‑101 (MQ=255)               tttttCACCTACCAGCGGCACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAg                                                                                  >  1:1545247/1‑101 (MQ=255)                ttttCACCTACCAGCGGCACTAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGt                                                                                 <  1:1579835/101‑1 (MQ=255)                ttttCACCTACCAGCGGCACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGt                                                                                 <  2:1545241/101‑1 (MQ=255)                 tttCACCTACCAGCGGCACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTg                                                                                <  2:1343138/101‑1 (MQ=255)                  ttCACCTACCAGCGGCACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTgg                                                                               <  2:1220475/101‑1 (MQ=255)                   tCACCTACCAGCGGCACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGc                                                                              <  1:1677360/101‑1 (MQ=255)                       cTACCAGCGGCACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCAc                                                                          >  1:889338/1‑101 (MQ=255)                       cTACCAGCGGCACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCAc                                                                          >  1:1334609/1‑101 (MQ=255)                        tACCAGCGGCACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACg                                                                         <  1:1659770/101‑1 (MQ=255)                          ccAGCGGCACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGc                                                                       <  2:105523/101‑1 (MQ=255)                            aGCGGCACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCag                                                                     >  1:860199/1‑101 (MQ=255)                              cGGCACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCagag                                                                   >  2:350917/1‑101 (MQ=255)                                 cACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTc                                                                <  2:1148070/101‑1 (MQ=255)                                 cACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTc                                                                <  1:1127052/101‑1 (MQ=255)                                  aCCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTcc                                                               >  2:337163/1‑101 (MQ=255)                                    cAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTcccc                                                             <  2:1565862/101‑1 (MQ=255)                                     aGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCa                                                            >  2:944699/1‑101 (MQ=255)                                        gtCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTg                                                         <  2:1479092/101‑1 (MQ=255)                                               tGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAg                                                  <  2:914098/101‑1 (MQ=255)                                                 gggCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGCGGAACAAGCt                                                >  2:669589/1‑101 (MQ=255)                                                    cGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGCCCCCAGTGGAACAAGCTGTa                                             >  2:1100824/1‑101 (MQ=255)                                                     gAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTaa                                            >  1:839714/1‑101 (MQ=255)                                                          aCTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGCCCCCAGTGGAACAAGCTGTAagccag                                       <  1:1610364/101‑1 (MQ=255)                                                           cTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTAagccagc                                      >  2:762804/1‑101 (MQ=255)                                                            ttCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTAagccagcc                                     >  1:1248182/1‑101 (MQ=255)                                                               cATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTAAGCCAGCCCCa                                  <  1:983715/101‑1 (MQ=255)                                                                aTTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTAAGCCAGCCCCaa                                 <  1:1504009/101‑1 (MQ=255)                                                                 ttttGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTAAGCCAGCCCCAAc                                <  1:1232203/101‑1 (MQ=255)                                                                  tttGCGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTAAGCCAGCCCCAAct                               >  2:553155/1‑101 (MQ=255)                                                                      cGGACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCANTGGAACAAGCTGTAANCCAGCNNCAACTCttt                           <  2:824906/101‑1 (MQ=255)                                                                       ggACAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTAAGCCAGCCCCAACTCTTTc                          >  1:590129/1‑101 (MQ=255)                                                                         aCAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTAAGCCAGCCCCAACTCTTTcgc                        >  2:1717414/1‑101 (MQ=255)                                                                          cAAAGAAGAAGTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTAAGCCAGCCCCAACTCTTTctcg                       >  2:1583765/1‑101 (MQ=255)                                                                             agaagaagTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTAAGCCAGCCCCAACTCTTTCGCGCCt                    >  1:752981/1‑101 (MQ=255)                                                                               aagaagTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTAAGCCAGCCCCAACTCTTTCGCGCCTGt                  >  2:1112689/1‑101 (MQ=255)                                                                               aagaagTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTAAGCCAGCCCCAACTCTTTCGCGCCTGt                  <  2:1689571/101‑1 (MQ=255)                                                                                agaagTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTAAGCCAGCCCCAACTCTTTCGCGCCTGTc                 >  1:420438/1‑101 (MQ=255)                                                                                 gaagTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTAAGCCAGCCCCAACTCTTTCGCGCCTGTCg                <  1:357280/101‑1 (MQ=255)                                                                                 gaagTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTAAGCCAGCCCCAACTCTTTCGCGCCTGTCg                <  2:1688173/101‑1 (MQ=255)                                                                                    gTAAGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTAAGCCAGCCCCAACTCTTTCGCGCCTGTCGAGt             >  2:393743/1‑101 (MQ=255)                                                                                       aGCGAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTAAGCCAGCCCCAACTCTTTCGCGCCTGTCGAGTTCg          >  2:973478/1‑101 (MQ=255)                                                                                          gAAGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTAAGCCAGCCCCAACTCTTTCGCGCCTGTCGAGTTCGGtt       >  2:514809/1‑101 (MQ=255)                                                                                           aaGGCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTAAGCCAGCCCCAACTCTTTCGCGCCTGTCGAGTTCGGTTc      >  2:289419/1‑101 (MQ=255)                                                                                              gCGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTAAGCCAGCCCCAACTCTTTCGCGCCTGTCGAGTTCGGTTCTaa   <  2:219104/101‑1 (MQ=255)                                                                                               cGATTGAAAGGAGGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTAAGCCAGCCCCAACTCTTTCGCGCCTGTCGAGTTCGGTTCTAAg  >  2:1421597/1‑101 (MQ=255)                                                                                               cGATTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTAAGCCAGCCCCAACTCTTTCGCGCCTGTCGAGTTCGGTTCTAAg  <  2:333044/101‑1 (MQ=255)                                                                                                  |                                                                                                  AACCCTTTGACGTGTTTTTCACCTACCAGCGGCACCAGTGTCGAGCTGGGGCGAATCACTTCCATTTTGCGGACAAAGAAGAAGTAAGCGAAGGCGACTGAAAGGAAGCTGTAAGTGGCCCACGGCAGAGGTCCCCAGTGGAACAAGCTGTAAGCCAGCCCCAACTCTTTCGCGCCTGTCGAGTTCGGTTCTAAG  >  57/2177‑2371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑