Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	13	17	G→T	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	13	17	0	G	T	100.0%	203.9 / NA	59	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (32/27);  total (32/27)

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCGTACATTGCGGCGGGGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGATACCAGCAGCACAGCGGCGACCGCAGACGCCAGCCCC  >  13/1‑111                                                                                                  |                                                                                               ccttgttgcaaatatcccactaataaaaccgcaatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGAt                                                                                             <  1:1007151‑M1/10‑1 (MQ=255) ccttgttgcaaatatcccactaataaaaccgcaatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGAt                                                                                             >  2:908648‑M1/92‑101 (MQ=255) ccttgttgcaaatatcccactaataaaaccgcaatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGAt                                                                                             <  2:489874‑M1/10‑1 (MQ=255) ccttgttgcaaatatcccactaataaaaccgcaatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGAt                                                                                             <  1:257603‑M1/10‑1 (MQ=255) ccttgttgcaaatatcccactaataaaaccgcaatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGAt                                                                                             >  1:23491‑M1/92‑101 (MQ=255) ccttgttgcaaatatcccactaataaaaccgcaatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGAt                                                                                             >  1:1623410‑M1/92‑101 (MQ=255)  cttgttgcaaatatcccactaataaaaccgcaatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATg                                                                                            >  2:1109779‑M1/91‑101 (MQ=255)   ttgttgcaaatatcccactaataaaaccgcaatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATgg                                                                                           <  2:55752‑M1/12‑1 (MQ=255)      ttgcaaatatcccactaataaaaccgcaatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGtgatgtgac                                                                                        >  2:679403‑M1/87‑97 (MQ=255)       tgcaaatatcccactaataaaaccgcaatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCggg                                                                                       >  2:257603‑M1/86‑101 (MQ=255)       agcaaatatcccactaataaaaccgcaatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCggg                                                                                       >  2:1352719‑M1/86‑101 (MQ=255)           aatatcccactaataaaaccgcaatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATc                                                                                   <  2:297371‑M1/20‑1 (MQ=255)            atatcccactaataaaaccgcaatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCa                                                                                  <  1:1118561‑M1/21‑1 (MQ=255)             tatcccactaataaaaccgcaatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAg                                                                                 >  1:107164‑M1/80‑101 (MQ=255)                 ccactaataaaaccgcaatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAac                                                                             <  2:73061‑M1/26‑1 (MQ=255)                 ccactaataaaaccgcaatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAac                                                                             >  1:1033345‑M1/76‑101 (MQ=255)                    ctaataaaaccgcaatggcggtgccgataatggagccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAacgac                                                                          >  1:814219‑M1/73‑101 (MQ=255)                     taataaaaccgcaatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAacgacg                                                                         <  1:496798‑M1/30‑1 (MQ=255)                      aataaaaccgcaatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGc                                                                        <  2:28767‑M1/31‑1 (MQ=255)                        taaaaccgcaatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGAcgcgc                                                                      <  1:841890‑M1/33‑3 (MQ=255)                        taaaaccgcaatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCAc                                                                      >  1:891622‑M1/69‑101 (MQ=255)                         aaaaccgcaatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCAcc                                                                     <  1:1656257‑M1/34‑1 (MQ=255)                              cgcaatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGAt                                                                <  2:1296217‑M1/39‑1 (MQ=255)                              cgcaatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGGGATGGCGGGCATCAGAAACGACGCACcgagaa                                                                <  2:717859‑M1/39‑2 (MQ=255)                                caatggcggtgccgataatggaaccggaatcaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCg                                                              <  2:1517893‑M1/41‑1 (MQ=255)                                caatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCg                                                              <  2:648321‑M1/41‑1 (MQ=255)                                caatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCg                                                              >  1:360954‑M1/61‑101 (MQ=255)                                 aatggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCgg                                                             >  2:709051‑M1/60‑101 (MQ=255)                                  atggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGa                                                            >  2:51181‑M1/59‑101 (MQ=255)                                    ggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAAc                                                          >  2:1566590‑M1/57‑101 (MQ=255)                                    ggcggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAAc                                                          >  1:387332‑M1/57‑101 (MQ=255)                                    ggaggtgccgaaaatggagccggaaccaacataaatattcgcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAAc                                                          >  2:819413‑M1/57‑101 (MQ=255)                                       ggtgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTg                                                       >  1:1439174‑M1/54‑101 (MQ=255)                                         tgccgataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCt                                                     <  1:591396‑M1/50‑1 (MQ=255)                                             gataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAAc                                                 <  1:96677‑M1/54‑1 (MQ=255)                                              ataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAAcc                                                <  2:1269500‑M1/55‑1 (MQ=255)                                              ataatggaaccggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAAcc                                                >  2:234078‑M1/47‑101 (MQ=255)                                                      accggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACg                                        >  2:1007146‑M1/39‑101 (MQ=255)                                                      accggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACg                                        <  2:815703‑M1/63‑1 (MQ=255)                                                        cggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGAt                                      <  1:1505792‑M1/65‑1 (MQ=255)                                                        cggaaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGAt                                      >  1:608097‑M1/37‑101 (MQ=255)                                                           aaccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGATAcc                                   <  2:1066820‑M1/68‑1 (MQ=255)                                                            accaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGATACca                                  >  1:1377869‑M1/33‑101 (MQ=255)                                                            accaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGATACca                                  >  2:464079‑M1/33‑101 (MQ=255)                                                             ccaccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGATACcag                                 >  2:121456‑M1/32‑101 (MQ=255)                                                                ccataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGATACcagcag                              <  1:1250369‑M1/73‑1 (MQ=255)                                                                 cataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGATACCagcagc                             >  1:297371‑M1/28‑101 (MQ=255)                                                                 cataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGATACCagcagc                             <  1:27479‑M1/74‑1 (MQ=255)                                                                  ataaatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGATACCAgcagca                            <  2:1419348‑M1/75‑1 (MQ=255)                                                                     aatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGATACCAGCAGCACAg                         >  2:1656251‑M1/24‑101 (MQ=255)                                                                     aatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGATACCAGCAGCACAg                         >  1:509860‑M1/24‑101 (MQ=255)                                                                     aatattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGATACCAGCAGCACAg                         >  2:1109404‑M1/24‑101 (MQ=255)                                                                       tattggcaccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGATACCAGCAGCACAgcg                       <  2:1175551‑M1/80‑1 (MQ=255)                                                                             caccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGATACCAGCAGCACAGCGGCGAcc                 >  2:74956‑M1/16‑101 (MQ=255)                                                                              accgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGATACCAGCAGCACAGCGGCGACCg                >  1:57914‑M1/15‑101 (MQ=255)                                                                              accgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGATACCAGCAGCACAGCGGCGACCg                <  2:1336270‑M1/87‑1 (MQ=255)                                                                               ccgccaagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGATACCAGCAGCACAGCGGCGACCGc               >  1:1269505‑M1/14‑101 (MQ=255)                                                                                   caagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGATACCAGCAGCACAGCGGCGACCGCAGAc           >  2:962226‑M1/10‑101 (MQ=255)                                                                                    aagcaccaCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGATACCAGCAGCACAGCGGCGACCGCAGACg          <  1:468009‑M1/93‑1 (MQ=255)                                                                                          caCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGATACCAGCAGCACAGCGGCGACCGCAGACGCCAGcc    <  1:318221‑M1/99‑1 (MQ=255)                                                                                          caCGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGATACCAGCAGCACAGCGGCGACCGCAGACGCCAGcc    <  2:946330‑M1/99‑1 (MQ=255)                                                                                            CGGCGGTGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGATACCAGCAGCACAGCGGCGACCGCAGACGCCAGcccc  >  2:193089‑M1/1‑101 (MQ=255)                                                                                                  |                                                                                               ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCGTACATTGCGGCGGGGATGGCGGGCATCAGAAACGACGCACCGAGATCGGAACGTGCTGAACCAAAATACGATACCAGCAGCACAGCGGCGACCGCAGACGCCAGCCCC  >  13/1‑111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑