Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	89	12,284	(GTCGCCAGC)1→2	intergenic (–/–)	– / –	–/–

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	89	12268 =	2 (0.030)	50 (0.930)	45/184	0.2	96.3%	intergenic (–/–)	–/–	–/–
	?	89	= 12276	2 (0.040)					intergenic (–/–)	–/–	–/–

TGCCATTGCGCGACGCGTTTGCGGTGCTGTTTTTTGTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  89/12375‑12268 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gctggcgaCGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCCCAACGTACGGCATTAACCATCGCCGCCAGCCT  <  89/12276‑12177                                                                                                                                                                                                                  TGCCATTGCGCGACGCGTTTGCGGTGCTGTTTTTTGTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACG                                                                                                             >  1:941587/1‑101         CGCGACGCGTTTGCGGTGCTGTTTTTTGTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGAC                                                                                                     >  2:537565/1‑101              CGCGTTTGCGGTGCTGTTTTTTGTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGG                                                                                                <  2:1223892/101‑1                 GTTTGCGGTGCTGTTTTTTGTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGA                                                                                             >  2:1112970/1‑101                   TTGCGGTGCTGTTTTTTGTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATT                                                                                           <  2:964934/101‑1                    TGCGGTGCTGTTTTTTGTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTA                                                                                          >  2:73042/1‑101                       GGTGCTGTTTTTTGTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGCTTCAGCAACCGCTGGCAGTGATGGCGACGCTGGGGGCGCTGGCGATTATTC                                                                                       >  2:478130/1‑101                        GTGCTGTTTTTTGTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCT                                                                                      >  2:1230291/1‑101                        GTGCTGTTTTTTGTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCT                                                                                      <  1:1448390/101‑1                        GTGCTGTTTTTTGTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCT                                                                                      >  2:252438/1‑101                         TGCTGTTTTTTGTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTG                                                                                     >  2:471807/1‑101                          GCTGTTTTTTGTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGT                                                                                    <  2:1085863/101‑1                            TGTTTTTTGTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTT                                                                                  >  1:416164/1‑101                               TTTTTGTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGT                                                                               <  2:941582/101‑1                                 TTTGTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAA                                                                             <  1:1625851/101‑1                                    GTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTC                                                                          >  2:219734/1‑101                                    GTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTC                                                                          <  1:609923/101‑1                                       TCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTT                                                                       >  1:521442/1‑101                                        CCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTA                                                                      >  1:1009205/1‑101                                         CGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAG                                                                     <  1:954453/101‑1                                         CGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAG                                                                     <  1:728558/101‑1                                           TCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCC                                                                   <  2:1328036/101‑1                                              GGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCA                                                                >  2:993805/1‑101                                               GATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCAT                                                               >  2:49214/1‑101                                                ATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATT                                                              >  1:677392/1‑101                                                 TGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTT                                                             <  1:673164/101‑1                                                  GTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTT                                                            <  2:676995/101‑1                                                  GTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTT                                                            <  2:921826/101‑1                                                  GTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTT                                                            >  1:469617/1‑101                                                      TTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCT                                                        <  1:1600401/101‑1                                                             CGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGTCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGA                                                 >  2:687949/1‑101                                                               TTAATTCTGATTCAGCATCCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACT                                               <  1:219734/101‑1                                                               TTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACT                                               >  2:1067863/1‑101                                                                  ATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTT                                            <  1:537570/101‑1                                                                  ATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTT                                            >  1:34014/1‑101                                                                     CTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGG                                         <  1:73042/101‑1                                                                       GATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTC                                       <  2:864363/101‑1                                                                         TTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCAC                                     <  1:632348/101‑1                                                                          TCAGCACCCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCCATTATTCTGTTTGGTAAGTCGTTAGCCGCCTTTTTCCTGGTGCGACTGTTTGGTCACT                                    <  2:882108/101‑1                                                                          TCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACT                                    <  2:469612/101‑1                                                                           CAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTC                                   >  1:535876/1‑101                                                                            AGCAACCGCTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCC                                  >  1:1431193/1‑101                                                                              CAACCGCTGGCAGTGCTGGCGACGCTGGCGATGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCCCA                                <  1:803968/101‑1                                                                                    CTGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCCCAACGTAC                          >  1:135914/1‑101                                                                                     TGGCAGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCCCAACGTACG                         <  1:889811/101‑1                                                                                         AGTGCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCCCAACGTACGGCAT                     >  1:620392/1‑101                                                                                            GCTGGCGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCCCAACGTACGGCATTAA                  <  2:1472031/101‑1                                                                                                 CGACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCCCAACGTACGGCATTAACCATC             <  1:142542/101‑1                                                                                                  GACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCCCAACGTACGGCATTAACCATCG            >  1:79915/1‑101                                                                                                  GACGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCCCAACGTACGGCATTAACCATCG            >  1:952765/1‑101                                                                                                    CGCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCCCAACGTACGGCATTAACCATCGCC          <  1:993810/101‑1                                                                                                     GCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCCCAACGTACGGCATTAACCATCGCCG         >  2:922346/1‑101                                                                                                     GCTGGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCCCAACGTACGGCATTAACCATCGCCG         >  2:1482841/1‑101                                                                                                        GGCGACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCCCAACGTACGGCATTAACCATCGCCGCCA      >  1:55298/1‑101                                                                                                            ACGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCCCAACGTACGGCATTAACCATCGCCGCCAGCCT  <  2:79915/101‑1                                                                                                                                                                                                                  TGCCATTGCGCGACGCGTTTGCGGTGCTGTTTTTTGTCTCCGTCGGGATGTTGTTTGATCCGTTAATTCTGATTCAGCAACCGCTGGCAGTGCTGGCGACGCTGGCGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  89/12375‑12268 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gctggcgaCGCTGGCGATTATTCTGTTTGGTAAGTCGTTAGCCGCATTTTTCCTGGTGCGACTGTTTGGTCACTCCCAACGTACGGCATTAACCATCGCCGCCAGCCT  <  89/12276‑12177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.