Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC JC	REL606	2,397,529	IS150 (–) Δ14 bp	coding (1124‑1137/2646 nt)	yfcTU ←	predicted outer membrane export usher protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	2397529	2397542	14	66 [0]	[0] 89	yfcTU	predicted outer membrane export usher protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 2397528	0 (0.000)	64 (1.000)	48/198	0.3	100%	coding (1138/2646 nt)	yfcTU	predicted outer membrane export usher protein
	?	REL606	2774435 =	NA (NA)					noncoding (1443/1443 nt)	IS150	repeat region
*	?	REL606	2397543 =	0 (0.000)	88 (1.400)	66/196	0.0	100%	coding (1123/2646 nt)	yfcTU	predicted outer membrane export usher protein
	?	REL606	= 2775877	NA (NA)					noncoding (1/1443 nt)	IS150	repeat region

ATCTCCCAGCGCGCCGCCATATAGCGACCAACCGTTAGCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCt‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/2397430‑2397528 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATT  >  REL606/2774435‑2774530                                                                                                                                                                                                     ATCTCCCAGCGCGCCGCCATATAGCGACCAACCGTTAGCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTG                                                                                                >  1:649133/1‑101  TCTCCCAGCGCGCCGCCATATAGCGACCAACCGTTAGCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGT                                                                                               >  1:1577727/1‑101  TCTCCCAGCGCGCCGCCATATAGCGACCAACCGTTAGCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGT                                                                                               >  2:126480/1‑101     CCCAGCGCGCCGCCATATAGCGACCAACCGTTAGCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACa                                                                                            >  1:1321594/1‑100     CCCAGCGCGCCGCCATATAGCGACCAACCGTTAGCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACT                                                                                            >  1:797318/1‑101     CCCAGCGCGCCGCCATATAGCGACCAACCGTTAGCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACT                                                                                            <  2:611128/101‑1       CAGCGCGCCGCCATATAGCGACCAACCGTTAGCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTaa                                                                                          >  1:1257613/1‑99       CAGCGCGCCGCCATATAGCGACCAACCGTTAGCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGc                                                                                          >  2:1623752/1‑100                GCCATATAGCGACCAACCGTTAGCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAA                                                                                 <  2:1682170/101‑1                GCCATATAGCGACCAACCGTTAGCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAA                                                                                 >  2:1043435/1‑101                GCCATATAGCGACCAACCGTTAGCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAA                                                                                 <  1:55026/101‑1                   ATATAGCGACCAACCGTTAGCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTT                                                                              >  2:1224653/1‑101                      TAGCGACCAACCGTTAGCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGA                                                                           <  1:1368386/101‑1                         CGACCAACCGTTAGCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAG                                                                        >  1:597040/1‑101                             CAACCGTTAGCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAA                                                                    >  2:844421/1‑101                             CAACCGTTAGCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAA                                                                    <  2:354076/101‑1                              AACCGTTAGCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAA                                                                   <  2:281134/101‑1                               ACCGTTAGCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAAC                                                                  <  1:1038338/101‑1                               ACCGTTAGCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAAC                                                                  <  1:601737/101‑1                                 CGTTAGCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACAC                                                                <  1:915288/101‑1                                    TAGCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAG                                                             <  2:618962/101‑1                                     AGCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGG                                                            <  2:206268/101‑1                                      GCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGC                                                           <  1:362995/101‑1                                        GATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCAT                                                         >  1:1620247/1‑101                                                 GGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTG                                                >  2:1365954/1‑101                                                   AGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGAT                                              >  1:368154/1‑101                                                   AGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGAT                                              >  2:414825/1‑101                                                   AGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTAACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGAT                                              <  2:1257608/101‑1                                                     GCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTC                                            <  2:1382146/101‑1                                                      CTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCC                                           >  1:955126/1‑101                                                      CTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCC                                           >  2:23606/1‑101                                                            CACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATT                                     >  2:1642925/1‑101                                                             ACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTC                                    >  2:1624408/1‑101                                                                AGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACAGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAAT                                 <  2:1321588/101‑1                                                                   GAAAAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGG                              <  1:1020949/101‑1                                                                      AAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGT                           >  2:1072394/1‑101                                                                      AAATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGT                           >  2:560163/1‑101                                                                       AATCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTC                          <  2:475232/101‑1                                                                         TCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAG                        >  1:1459279/1‑101                                                                         TCCCCCCTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAG                        <  2:269013/101‑1                                                                               CTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTT                  <  1:1336601/101‑1                                                                               CTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTT                  >  2:460819/1‑101                                                                               CTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTT                  >  1:786759/1‑101                                                                               CTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTT                  >  1:358497/1‑101                                                                               CTCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTT                  >  1:657794/1‑101                                                                                TCGACATGGTGGCCCCACTCTGTACTGCCCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTT                 <  1:1169707/101‑1                                                                                TCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTT                 <  1:677060/101‑1                                                                                TCGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTT                 <  1:461595/101‑1                                                                                 gaACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTA                >  2:1023410/3‑101                                                                                 CGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGTCCTTTTA                >  2:1250797/1‑101                                                                                 CGACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTA                <  2:357262/101‑1                                                                                  GACATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAA               >  1:1546565/1‑101                                                                                    CATGGTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATT             >  1:1231174/1‑101                                                                                        GTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAG         <  2:396305/101‑1                                                                                        GTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAG         >  1:729903/1‑101                                                                                        GTGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAG         <  1:939168/101‑1                                                                                         TGGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGG        <  2:1638328/101‑1                                                                                          GGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGC       <  2:1597352/101‑1                                                                                          GGCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGC       >  1:956307/1‑101                                                                                           GCCCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCT      >  2:145021/1‑101                                                                                            annnnCTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTA     >  2:7620/6‑101                                                                                             CCCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAA    >  1:1061714/1‑101                                                                                              CCACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAAT   <  2:804899/101‑1                                                                                               CACTCTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATT  >  2:878050/1‑101                                                                                                                                                                                                     ATCTCCCAGCGCGCCGCCATATAGCGACCAACCGTTAGCGATCCCCCAGGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCt‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/2397430‑2397528 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATT  >  REL606/2774435‑2774530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.