BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	REL606	4,459,658	A→G	100%	P315P (CCA→CCG)	yjgL →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,459,658	0	A	G	100.0%	546.7 / NA	141	P315P (CCA→CCG)	yjgL	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (63/78); total (63/78)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 152 total aligned reads displayed.

CCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGAAT  >  REL606/4459610‑4459705

 cTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCgtat                                              <  1:2855901/51‑1 (MQ=255)

  ttACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCgtata                                             <  1:202619/51‑1 (MQ=255)

  ttACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCgtata                                             <  1:3863100/51‑1 (MQ=255)

   tACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCgtatat                                            <  1:8698617/51‑1 (MQ=255)

   tACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCgtatat                                            <  1:867815/51‑1 (MQ=255)

   tACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCgtatat                                            >  1:10609790/1‑51 (MQ=255)

    acacCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATAtt                                           <  1:6811410/51‑1 (MQ=255)

    acacCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATAtt                                           <  1:5987205/51‑1 (MQ=255)

     cacCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTa                                          <  1:5545902/51‑1 (MQ=255)

     cacCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTa                                          <  1:6970316/51‑1 (MQ=255)

     cacCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTa                                          <  1:744390/51‑1 (MQ=255)

     cacCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTa                                          <  1:8109731/51‑1 (MQ=255)

      acCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTaa                                         <  1:7639107/51‑1 (MQ=255)

      acCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTaa                                         >  1:6353654/1‑51 (MQ=255)

        cGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAatg                                       >  1:6477779/1‑51 (MQ=255)

        cGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAatg                                       >  1:4366073/1‑51 (MQ=255)

        cGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAatg                                       >  1:7293729/1‑51 (MQ=255)

        cGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAatg                                       <  1:7683672/51‑1 (MQ=255)

        cGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAatg                                       <  1:6062776/51‑1 (MQ=255)

         ggTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAatga                                      <  1:823869/51‑1 (MQ=255)

         ggTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAatga                                      <  1:6489759/51‑1 (MQ=255)

          gTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAatgat                                     <  1:3381981/51‑1 (MQ=255)

          gTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAatgat                                     >  1:7040627/1‑51 (MQ=255)

             aTCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTa                                  >  1:2167150/1‑51 (MQ=255)

             aTCGATTCGATTCTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTa                                  <  1:5249996/51‑1 (MQ=255)

              tcgattcgatTCTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTaa                                 >  1:1702878/1‑51 (MQ=255)

               cgattcgattCTGGAGACTTTTTTAAAATCCCCGTATATTAatg                                       <  1:10261730/44‑1 (MQ=255)

               cgattcgattCTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAg                                <  1:9554848/51‑1 (MQ=255)

               cgattcgattCTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAg                                <  1:8133749/51‑1 (MQ=255)

                gattcgattcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGa                               >  1:1365405/1‑51 (MQ=255)

                gattcgattcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGa                               >  1:15469/1‑51 (MQ=255)

                gattcgattcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGa                               >  1:6582356/1‑51 (MQ=255)

                gattcgattcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGa                               <  1:1093459/51‑1 (MQ=255)

                gattcgattcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGa                               >  1:2281248/1‑51 (MQ=255)

                 attcgattcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGaa                              >  1:2679990/1‑51 (MQ=255)

                 attcgattcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGaa                              <  1:626228/51‑1 (MQ=255)

                 attcgattcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGaa                              >  1:2495821/1‑51 (MQ=255)

                  ttcgattcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAAt                             >  1:1548253/1‑51 (MQ=255)

                  ttcgattcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAAt                             >  1:6278362/1‑51 (MQ=255)

                   tcgattcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAAt                             <  1:6217551/50‑1 (MQ=255)

                   tcgattcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATa                            <  1:10320165/51‑1 (MQ=255)

                   tcgattcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATa                            <  1:9431315/51‑1 (MQ=255)

                   tcgattcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATa                            <  1:8002844/51‑1 (MQ=255)

                   tcgattcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATa                            >  1:9311205/1‑51 (MQ=255)

                   tcgattcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATa                            >  1:7283564/1‑51 (MQ=255)

                    cgattcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATAc                           <  1:9170603/51‑1 (MQ=255)

                    cgattcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATAc                           <  1:87890/51‑1 (MQ=255)

                    cgattcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATAc                           <  1:1736178/51‑1 (MQ=255)

                     gattcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACt                          <  1:3589706/51‑1 (MQ=255)

                      attcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTg                         <  1:9269085/51‑1 (MQ=255)

                      attcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTg                         >  1:8525088/1‑51 (MQ=255)

                      attcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTg                         <  1:9122369/51‑1 (MQ=255)

                       ttcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTgg                        >  1:5736653/1‑51 (MQ=255)

                       ttcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTgg                        >  1:1956306/1‑51 (MQ=255)

                       ttcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTgg                        <  1:9706533/51‑1 (MQ=255)

                       ttcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTgg                        <  1:4884776/51‑1 (MQ=255)

                       ttcTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTgg                        <  1:7091366/51‑1 (MQ=255)

                         cTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGAt                      <  1:5520559/51‑1 (MQ=255)

                         cTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGAt                      >  1:7140073/1‑51 (MQ=255)

                         cTGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGAt                      >  1:4908114/1‑51 (MQ=255)

                          tGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGAtt                     <  1:793946/51‑1 (MQ=255)

                          tGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGAtt                     >  1:1660780/1‑51 (MQ=255)

                          tGGAGACTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGAtt                     <  1:7498312/51‑1 (MQ=255)

                             agaCTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGgt                  >  1:4601791/1‑51 (MQ=255)

                              gaCTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGgtg                 >  1:7140871/1‑51 (MQ=255)

                               aCTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGgtgt                >  1:5325178/1‑51 (MQ=255)

                                cTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGtt               >  1:18695/1‑51 (MQ=255)

                                cTTTTTTAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGtt               >  1:1329934/1‑51 (MQ=255)

                                 ttttttAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGttt              >  1:7993699/1‑51 (MQ=255)

                                 ttttttAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGttt              <  1:3949361/51‑1 (MQ=255)

                                  tttttAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGtttt             <  1:1009251/51‑1 (MQ=255)

                                  tttttAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGtttt             <  1:3930599/51‑1 (MQ=255)

                                    tttAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTtaa           <  1:3770331/51‑1 (MQ=255)

                                    tttAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTtaa           <  1:3972577/51‑1 (MQ=255)

                                    tttAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTtaa           <  1:202949/51‑1 (MQ=255)

                                    tttAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTtaa           >  1:3624413/1‑51 (MQ=255)

                                    tttAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTtaa           >  1:5221701/1‑51 (MQ=255)

                                    tttAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTtaa           <  1:1667721/51‑1 (MQ=255)

                                     ttAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTtaat          >  1:5584273/1‑51 (MQ=255)

                                     ttAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTtaat          >  1:9355664/1‑51 (MQ=255)

                                      tAAAATCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTtaata         <  1:7542700/51‑1 (MQ=255)

                                       aaaaTCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTtaataa        <  1:2301151/51‑1 (MQ=255)

                                       aaaaTCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTtaataa        >  1:2675122/1‑51 (MQ=255)

                                       aaaaTCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTtaataa        >  1:4344157/1‑51 (MQ=255)

                                        aaaTCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATaaa       <  1:337993/51‑1 (MQ=255)

                                         aaTCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATaaaa      <  1:9575973/51‑1 (MQ=255)

                                         aaTCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATaaaa      >  1:4817939/1‑51 (MQ=255)

                                         aaTCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATaaaa      <  1:8221309/51‑1 (MQ=255)

                                          aTCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAGTAAAAg     >  1:6485282/1‑51 (MQ=39)

                                          aTCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAg     <  1:5373325/51‑1 (MQ=255)

                                           tCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGa    >  1:7930909/1‑51 (MQ=255)

                                           tCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGa    <  1:355392/51‑1 (MQ=255)

                                           tCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGa    <  1:6075533/51‑1 (MQ=255)

                                           tCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGa    <  1:1957226/51‑1 (MQ=255)

                                           tCCCCGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGa    >  1:2147593/1‑51 (MQ=255)

                                            ccccGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGaa   >  1:1879642/1‑51 (MQ=255)

                                            ccccGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGaa   <  1:8840666/51‑1 (MQ=255)

                                            ccccGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGaa   >  1:2764715/1‑51 (MQ=255)

                                            ccccGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGaa   <  1:840088/51‑1 (MQ=255)

                                            ccccGTATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGa    <  1:10416747/50‑1 (MQ=255)

CCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTTAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGAAT  >  REL606/4459610‑4459705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`