BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	REL606	4,096,877	G→T	100%	Y232* (TAC→TAA)	glpF ←	glycerol facilitator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,096,877	0	G	T	100.0%	411.0 / NA	116	Y232* (TAC→TAA)	glpF	glycerol facilitator
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (68/48); total (68/48)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 116 total aligned reads displayed.

TGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCG  >  REL606/4096829‑4096923

tGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTa                                              >  1:9499312/1‑51 (MQ=255)

tGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTa                                              >  1:4339373/1‑51 (MQ=255)

tGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTa                                              <  1:7679675/51‑1 (MQ=255)

 gCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTaa                                             >  1:7769281/1‑51 (MQ=255)

 gCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTaa                                             <  1:7760437/51‑1 (MQ=255)

 gCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTaa                                             <  1:5069512/51‑1 (MQ=255)

 gCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTaa                                             >  1:3082456/1‑51 (MQ=255)

 gCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTaa                                             >  1:6265354/1‑51 (MQ=255)

 gCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTaa                                             >  1:5688306/1‑51 (MQ=255)

 gCACCTACAATCACGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTaa                                             >  1:897906/1‑51 (MQ=255)

  cACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAg                                            <  1:7021307/51‑1 (MQ=255)

  cACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAg                                            >  1:5127828/1‑51 (MQ=255)

  cACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAg                                            >  1:10151384/1‑51 (MQ=255)

  cACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAg                                            <  1:553101/51‑1 (MQ=255)

   aCCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAgg                                           <  1:5024290/51‑1 (MQ=255)

   aCCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAgg                                           <  1:7503519/51‑1 (MQ=255)

   aCCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAgg                                           >  1:2193920/1‑51 (MQ=255)

   aCCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAgg                                           <  1:2891384/51‑1 (MQ=255)

   aCCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAgg                                           <  1:2045528/51‑1 (MQ=255)

    ccTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGa                                          <  1:5184378/51‑1 (MQ=255)

     cTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGaa                                         <  1:3316613/51‑1 (MQ=255)

     cTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGaa                                         <  1:2901090/51‑1 (MQ=255)

      tACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCGGGAATTAAGGAAt                                        <  1:1653684/51‑1 (MQ=39)

      tACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAAt                                        <  1:6706202/51‑1 (MQ=255)

       aCAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATg                                       <  1:3919317/51‑1 (MQ=255)

       aCAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATg                                       >  1:8301619/1‑51 (MQ=255)

       aCAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATg                                       <  1:8785035/51‑1 (MQ=255)

        cAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGt                                      >  1:613577/1‑51 (MQ=255)

         aaTCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGtc                                     >  1:9178438/1‑51 (MQ=255)

         aaTCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGtc                                     <  1:5941922/51‑1 (MQ=255)

          aTCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGtct                                    >  1:10913316/1‑51 (MQ=255)

           tCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGtctc                                   <  1:2053188/51‑1 (MQ=255)

            cgcgCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTctct                                  >  1:1996280/1‑51 (MQ=255)

            cgcgCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTctct                                  >  1:9493218/1‑51 (MQ=255)

             gcgcCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTg                                 >  1:1682711/1‑51 (MQ=255)

             gcgcCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTg                                 >  1:7642498/1‑51 (MQ=255)

              cgcCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTgc                                >  1:10624481/1‑51 (MQ=255)

              cgcCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTgc                                >  1:10799675/1‑51 (MQ=255)

              cgcCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTgc                                >  1:3910204/1‑51 (MQ=255)

               gcCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTgcc                               >  1:4529083/1‑51 (MQ=255)

               gcCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTgcc                               >  1:8787828/1‑51 (MQ=255)

                ccAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTgccg                              >  1:4149491/1‑51 (MQ=255)

                 cAACGATAGGGGCAAAAAGCGGCTCCAGGAATTAAGGAATGTCTCTgccgc                             <  1:10872412/51‑1 (MQ=255)

                 cAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTgccgc                             >  1:3758929/1‑51 (MQ=255)

                 cAACGATAGGGCCAAAAAGCAGCACCAGGAATTAAGGAATGTCTCTgccgc                             >  1:9369599/1‑51 (MQ=255)

                  aaCGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTgccgcc                            >  1:4389136/1‑51 (MQ=255)

                  aaCGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTgccgcc                            >  1:3914395/1‑51 (MQ=255)

                   aCGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGccgccg                           >  1:6287005/1‑51 (MQ=255)

                   aCGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGccgccg                           <  1:1913381/51‑1 (MQ=255)

                    cGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCgg                          >  1:662450/1‑51 (MQ=255)

                    cGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCgg                          <  1:8950042/51‑1 (MQ=255)

                    cGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCgg                          <  1:3881931/51‑1 (MQ=255)

                    cGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCgg                          >  1:7178930/1‑51 (MQ=255)

                    cGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCgg                          <  1:2731770/51‑1 (MQ=255)

                    cGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCgg                          <  1:6217498/51‑1 (MQ=255)

                    cGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCgg                          <  1:580761/51‑1 (MQ=255)

                    cGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCgg                          >  1:3271764/1‑51 (MQ=255)

                     gATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGt                         >  1:8338490/1‑51 (MQ=255)

                      aTAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTa                        >  1:5750247/1‑51 (MQ=255)

                       tAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTaa                       >  1:167025/1‑51 (MQ=255)

                        aGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTaaa                      <  1:10090249/51‑1 (MQ=255)

                        aGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTaaa                      <  1:3336111/51‑1 (MQ=255)

                         gggCCAAAAAGCGGCACCGGGAATTAAGGAATGTCTCTGCCGCCGGTAAAg                     <  1:2749452/51‑1 (MQ=39)

                         gggCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAg                     <  1:3389748/51‑1 (MQ=255)

                          ggCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAgg                    >  1:9241305/1‑51 (MQ=255)

                          ggCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAgg                    >  1:8858910/1‑51 (MQ=255)

                          ggCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAgg                    >  1:1230717/1‑51 (MQ=255)

                           gCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGc                   >  1:1321895/1‑51 (MQ=255)

                            ccAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCg                  >  1:1813506/1‑51 (MQ=255)

                            ccAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCg                  >  1:6614772/1‑51 (MQ=255)

                            ccAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCg                  >  1:10703061/1‑51 (MQ=255)

                            ccAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCg                  >  1:7120434/1‑51 (MQ=255)

                             cAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGa                 >  1:6267103/1‑51 (MQ=255)

                             cAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGa                 >  1:8431408/1‑51 (MQ=255)

                              aaaaaGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTaa                       <  1:2220529/44‑1 (MQ=255)

                               aaaaGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACa               <  1:812771/51‑1 (MQ=255)

                                aaaGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACAt              >  1:8473107/1‑51 (MQ=255)

                                aaaGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACAt              <  1:6245935/51‑1 (MQ=255)

                                aaaGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACAt              >  1:457115/1‑51 (MQ=255)

                                 aaGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACAtt             <  1:9026902/51‑1 (MQ=255)

                                 aaGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACAtt             <  1:9300473/51‑1 (MQ=255)

                                  aGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTg            <  1:5442245/51‑1 (MQ=255)

                                   gCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGc           >  1:7994639/1‑51 (MQ=255)

                                   gCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGc           >  1:10178402/1‑51 (MQ=255)

                                     ggCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTg            >  1:6515398/1‑48 (MQ=255)

                                     ggCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGccc         <  1:2971293/51‑1 (MQ=255)

                                      gCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGcccc        >  1:2561392/1‑51 (MQ=255)

                                      gCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGcccc        <  1:313353/51‑1 (MQ=255)

                                      gCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGccc         <  1:10759462/50‑1 (MQ=255)

                                       cACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCa       >  1:464390/1‑51 (MQ=255)

                                        aCCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAg      >  1:279689/1‑51 (MQ=255)

                                         ccAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGc     >  1:10560241/1‑51 (MQ=255)

                                         ccAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGc     <  1:7732375/51‑1 (MQ=255)

                                          cAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGcc    >  1:7237276/1‑51 (MQ=255)

                                          cAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGcc    <  1:2378899/51‑1 (MQ=255)

                                           aGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGccc   >  1:1283664/1‑51 (MQ=255)

                                           aGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGccc   <  1:5205421/51‑1 (MQ=255)

                                           aGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGccc   <  1:1680780/51‑1 (MQ=255)

                                            ggAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCg  <  1:4451029/51‑1 (MQ=255)

                                            ggAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCg  >  1:615106/1‑51 (MQ=255)

TGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCG  >  REL606/4096829‑4096923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`