| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 3,328,272 | A→G | 100% | V236A (GTT→GCT) | mreB ← | cell wall structural complex MreBCD, actin‑like component MreB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,328,272 | 0 | A | G | 99.2% | 438.2 / ‑4.9 | 116 | V236A (GTT→GCT) | mreB | cell wall structural complex MreBCD, actin‑like component MreB |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (47/68); total (48/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 116 total aligned reads displayed.
TTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCG > REL606/3328224‑3328319 | ttCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCt > 1:1953388/1‑51 (MQ=255) cAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTc > 1:6009936/1‑51 (MQ=255) cAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTc < 1:2476202/51‑1 (MQ=255) cAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTc < 1:1525370/51‑1 (MQ=255) cAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTc < 1:5709653/51‑1 (MQ=255) cAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTc > 1:2013035/1‑51 (MQ=255) cAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTc > 1:3075983/1‑51 (MQ=255) cAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTc > 1:8984963/1‑51 (MQ=255) cAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTc > 1:1259089/1‑51 (MQ=255) aGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCg < 1:10362507/51‑1 (MQ=255) gggTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGa < 1:4255198/51‑1 (MQ=255) ggTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGAt < 1:3899928/51‑1 (MQ=255) ggTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGAt > 1:3066418/1‑51 (MQ=255) ggTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGAt > 1:5801350/1‑51 (MQ=255) gTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGAtt < 1:4564830/51‑1 (MQ=255) tAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGAttt < 1:2260914/51‑1 (MQ=255) tAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGAttt > 1:864548/1‑51 (MQ=255) aaaaCCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTc < 1:9197355/51‑1 (MQ=255) aaaCCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCa < 1:4630237/51‑1 (MQ=255) aaaCCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCa < 1:8484096/51‑1 (MQ=255) aaaCCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCa < 1:6082886/51‑1 (MQ=255) aaCCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCAc > 1:10296766/1‑51 (MQ=255) aaCCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCAc < 1:8329316/51‑1 (MQ=255) aaCCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCAc < 1:4906729/51‑1 (MQ=255) cgTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACt > 1:9757042/1‑51 (MQ=255) cgTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACt < 1:1318058/51‑1 (MQ=255) cgTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACt > 1:7006102/1‑51 (MQ=255) gTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACtt < 1:3385737/51‑1 (MQ=255) gTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACtt < 1:1055500/51‑1 (MQ=255) tGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTc < 1:8231883/51‑1 (MQ=255) tGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTc > 1:7844287/1‑51 (MQ=255) tGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTc < 1:3411963/51‑1 (MQ=255) tGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGACTTCACGGACTTc < 1:5981704/51‑1 (MQ=38) ggAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCa > 1:10118145/1‑51 (MQ=255) gAACTCCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCAt < 1:9867159/51‑1 (MQ=255) gAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCAt > 1:10176979/1‑51 (MQ=255) gAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCAt < 1:1300764/51‑1 (MQ=255) aaCACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATc < 1:9493168/51‑1 (MQ=255) aaCACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATc < 1:5346489/51‑1 (MQ=255) acacCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCg > 1:17784/1‑51 (MQ=255) acacCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCg > 1:2776712/1‑51 (MQ=255) acacCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCg < 1:310603/51‑1 (MQ=255) acacCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCg > 1:7458143/1‑51 (MQ=255) acacCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCg < 1:6747698/51‑1 (MQ=255) cacCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCg < 1:4168385/50‑1 (MQ=255) cacCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGc < 1:748511/51‑1 (MQ=255) cacCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGc > 1:9005993/1‑51 (MQ=255) acCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGcc > 1:9889688/1‑51 (MQ=255) acCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGcc > 1:1886220/1‑51 (MQ=255) ccTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGccc < 1:6704048/51‑1 (MQ=255) ccTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGccc < 1:8561055/51‑1 (MQ=255) ccTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGccc < 1:7474227/51‑1 (MQ=255) cTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCg < 1:2622942/51‑1 (MQ=255) ttCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCgg > 1:7137118/1‑51 (MQ=255) tCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGa < 1:8780830/51‑1 (MQ=255) tCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCAGGa > 1:3645736/1‑51 (MQ=255) cTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGAt < 1:218977/51‑1 (MQ=255) cTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGAt > 1:10487565/1‑51 (MQ=255) cTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGAt < 1:480822/51‑1 (MQ=255) tGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATa < 1:8610887/51‑1 (MQ=255) tGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATa < 1:10445837/51‑1 (MQ=255) gCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATaa < 1:3171981/51‑1 (MQ=255) gCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATaa > 1:6619885/1‑51 (MQ=255) gCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATaa < 1:1578318/51‑1 (MQ=255) gCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATaa < 1:2914495/51‑1 (MQ=255) gCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATaa < 1:6861725/51‑1 (MQ=255) gCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATaa < 1:6813345/51‑1 (MQ=255) ccAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAg > 1:3534678/1‑51 (MQ=255) ccAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAg > 1:9603782/1‑51 (MQ=255) ccAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAg < 1:9328414/51‑1 (MQ=255) ccAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAg > 1:410603/1‑51 (MQ=255) cAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGc < 1:5653157/51‑1 (MQ=255) cAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGc < 1:500761/51‑1 (MQ=255) aGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATCAGcc > 1:2869863/1‑51 (MQ=255) aGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGcc < 1:4570946/51‑1 (MQ=255) aGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGcc > 1:9846187/1‑51 (MQ=255) aGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGcc < 1:8218006/51‑1 (MQ=255) aGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGcc > 1:464239/1‑51 (MQ=255) gTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGa < 1:9305470/51‑1 (MQ=255) gTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGa < 1:7360934/51‑1 (MQ=255) ttACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGaa > 1:5242467/1‑51 (MQ=255) aCGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGa < 1:8606195/48‑1 (MQ=255) aCGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAAcc > 1:193718/1‑51 (MQ=255) cGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCg < 1:3555483/51‑1 (MQ=255) ggCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGa > 1:6700627/1‑51 (MQ=255) ggCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGa < 1:8632742/51‑1 (MQ=255) ggCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGa > 1:9872844/1‑51 (MQ=255) ggCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGa > 1:528154/1‑51 (MQ=255) ggCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGa < 1:7923984/51‑1 (MQ=255) ccACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGAtt < 1:6187159/51‑1 (MQ=255) ccACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGAtt < 1:372550/51‑1 (MQ=255) cACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGAttt < 1:8617226/51‑1 (MQ=255) cACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGAttt < 1:5768121/51‑1 (MQ=255) cACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGAttt < 1:252211/51‑1 (MQ=255) cACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGAt > 1:10858182/1‑49 (MQ=255) aCGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTc < 1:6442046/51‑1 (MQ=255) aCGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTc < 1:721505/51‑1 (MQ=255) aCGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTc < 1:498963/51‑1 (MQ=255) aCGAGCTTCGATTTCACGGACTTCATCGCCCAGATAAGCCGAACCGATTTc < 1:3071952/51‑1 (MQ=255) cGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCg < 1:2040300/51‑1 (MQ=255) | TTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCG > REL606/3328224‑3328319 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |