| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 3,093,020 | T→G | 100% | F74L (TTT→TTG) | yqhH → | predicted outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,093,020 | 0 | T | G | 99.2% | 468.2 / ‑4.2 | 122 | F74L (TTT→TTG) | yqhH | predicted outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (55/66); total (56/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 127 total aligned reads displayed.
ATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTTGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCC > REL606/3092972‑3093067 | aTCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGa < 1:4781639/51‑1 (MQ=255)aTCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGa < 1:214205/51‑1 (MQ=255) tCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTTGAt > 1:8920043/1‑51 (MQ=255) tCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGAt > 1:2829619/1‑51 (MQ=255) tCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGAt < 1:3141419/51‑1 (MQ=255) tCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGAt > 1:9205291/1‑51 (MQ=255) tCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGAt > 1:5281505/1‑51 (MQ=255) ccGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGAtt > 1:5147010/1‑51 (MQ=255) ccGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGAtt < 1:28562/51‑1 (MQ=255) ccGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGAtt < 1:7810031/51‑1 (MQ=255) cGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTg > 1:10948644/1‑51 (MQ=255) cGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTg > 1:9836803/1‑51 (MQ=255) cGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTg > 1:8818079/1‑51 (MQ=255) gNAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGc > 1:372941/1‑51 (MQ=255) gAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGc > 1:9225251/1‑51 (MQ=255) gAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGc > 1:1174344/1‑51 (MQ=255) gCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTg < 1:4332470/51‑1 (MQ=255) cTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTgc < 1:5674380/51‑1 (MQ=255) cTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTgc < 1:2710408/51‑1 (MQ=255) cTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTgc < 1:9656834/51‑1 (MQ=255) cTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTgc < 1:5680638/51‑1 (MQ=255) tAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTgcg < 1:5132348/51‑1 (MQ=255) tAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTgcg > 1:5191330/1‑51 (MQ=255) aaCAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTgcgc > 1:3764373/1‑51 (MQ=255) aaCAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTgcgc > 1:4268431/1‑51 (MQ=255) aCAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCgct < 1:4563704/51‑1 (MQ=255) cAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCgctg < 1:2087464/51‑1 (MQ=255) cAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCgctg > 1:7181799/1‑51 (MQ=255) agagCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCgctgc > 1:3302440/1‑51 (MQ=255) agagCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCgctgc > 1:1360910/1‑51 (MQ=255) agCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCtt < 1:2980476/51‑1 (MQ=255) agCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCtt < 1:6043871/51‑1 (MQ=255) agCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCtt < 1:8441869/51‑1 (MQ=255) agCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCtt < 1:1612729/51‑1 (MQ=255) ccAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCgctg < 1:3542305/46‑1 (MQ=255) cAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCgctgc < 1:984474/46‑1 (MQ=255) cAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCg > 1:8404151/1‑51 (MQ=255) aaTACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGt < 1:6539575/51‑1 (MQ=255) aaTACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGt < 1:8520411/51‑1 (MQ=255) aTACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTa > 1:7589747/1‑51 (MQ=255) aTACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTa < 1:1226080/51‑1 (MQ=255) tACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTAt > 1:2245228/1‑51 (MQ=255) tACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTAt > 1:10604528/1‑51 (MQ=255) tACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTAt > 1:6018684/1‑51 (MQ=255) aCGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATg > 1:1171976/1‑51 (MQ=255) cgcgTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGt < 1:242897/51‑1 (MQ=255) cgcgTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGt < 1:1387442/51‑1 (MQ=255) gcgTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTa < 1:6201018/51‑1 (MQ=255) gcgTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTa < 1:8275325/51‑1 (MQ=255) cgTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTAc > 1:4958781/1‑51 (MQ=255) cgTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTAc > 1:8317242/1‑51 (MQ=255) cgTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTAc < 1:7693265/51‑1 (MQ=255) cgTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTAc < 1:8622572/51‑1 (MQ=255) gTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACg < 1:7579584/51‑1 (MQ=255) gTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACg < 1:3405725/51‑1 (MQ=255) gTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACg < 1:2541426/51‑1 (MQ=255) gTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACg < 1:1688276/51‑1 (MQ=255) gTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACg > 1:2287318/1‑51 (MQ=255) gTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACg > 1:378898/1‑51 (MQ=255) tCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGc < 1:6414082/51‑1 (MQ=255) tCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGc > 1:1424741/1‑51 (MQ=255) cTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCa < 1:9462755/51‑1 (MQ=255) ttGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAg < 1:5812728/51‑1 (MQ=255) ttGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAg < 1:4285717/51‑1 (MQ=255) ttGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAg > 1:9272077/1‑51 (MQ=255) ttGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAg < 1:4871262/51‑1 (MQ=255) tGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGa < 1:7818832/51‑1 (MQ=255) tGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGa > 1:5301112/1‑51 (MQ=255) gATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGaa < 1:6225784/51‑1 (MQ=255) aTGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAAt > 1:9307399/1‑51 (MQ=255) tGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATg > 1:9721803/1‑51 (MQ=255) cTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGAt > 1:1349972/1‑51 (MQ=255) cTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGAt > 1:6473406/1‑51 (MQ=255) tCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATa < 1:7818285/51‑1 (MQ=255) tCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATa < 1:6992329/51‑1 (MQ=255) cAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATaa < 1:5560756/51‑1 (MQ=255) cAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATaa < 1:5927145/51‑1 (MQ=255) cAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATaa < 1:10191379/51‑1 (MQ=255) aGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATaaa < 1:2429255/51‑1 (MQ=255) aGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATaaa > 1:3258130/1‑51 (MQ=255) aGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATaaa > 1:1259762/1‑51 (MQ=255) ggACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATaaaa < 1:6816402/51‑1 (MQ=255) ggACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATaaaa < 1:5747644/51‑1 (MQ=255) aCTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATaaaaaa < 1:5549920/51‑1 (MQ=255) aCTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATaaaaaa < 1:1912600/51‑1 (MQ=255) aCTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATaaaaaa < 1:8521265/51‑1 (MQ=255) aCTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATaaaaaa < 1:10640272/51‑1 (MQ=255) cTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATaaaaaaa > 1:4259799/1‑51 (MQ=255) tATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAAt < 1:1844397/51‑1 (MQ=255) tATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAAt > 1:3574724/1‑51 (MQ=255) aTTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATc < 1:828430/51‑1 (MQ=255) aTTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATc < 1:9768239/51‑1 (MQ=255) aTTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATc < 1:8992500/51‑1 (MQ=255) aTTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATc > 1:3510995/1‑51 (MQ=255) aTTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATc < 1:9725856/51‑1 (MQ=255) aTTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATc > 1:7259781/1‑51 (MQ=255) tttgGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATcc < 1:2255949/51‑1 (MQ=255) tttgGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATcc < 1:1290852/51‑1 (MQ=255) tttgGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATcc > 1:1405410/1‑51 (MQ=255) tttgGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATcc > 1:10188490/1‑51 (MQ=255) | ATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTTGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCC > REL606/3092972‑3093067 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |