| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 3,067,926 | G→A | 100% | A40V (GCA→GTA) | yghS ← | predicted protein with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,067,926 | 0 | G | A | 99.2% | 376.8 / ‑5.3 | 110 | A40V (GCA→GTA) | yghS | predicted protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (69/40); total (69/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 116 total aligned reads displayed.
ATTCGCCCGGACGATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTTGCTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGATTTACCTGAA > REL606/3067878‑3067973 | aTTCGCCCGGACGATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTtact < 1:6112183/51‑1 (MQ=255) ttCGCCCGGACGATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTtactg < 1:6090288/51‑1 (MQ=255) ttCGCCCGGACGATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTtactg < 1:3409742/51‑1 (MQ=255) ttCGCCCGGACGATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTtactg > 1:4097589/1‑51 (MQ=255) tCGCCCGGACGATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTtactgc > 1:9303749/1‑51 (MQ=255) gCCCGGACGATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCa > 1:1099011/1‑51 (MQ=255) gCCCGGACGATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCa > 1:3181213/1‑51 (MQ=255) cccGGACGATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAg < 1:2713634/51‑1 (MQ=255) cccGGACGATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAg > 1:5331681/1‑51 (MQ=255) cccGGACGATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAg > 1:1256494/1‑51 (MQ=255) cccGGACGATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAg < 1:7747212/51‑1 (MQ=255) ccGGACGATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGt > 1:1260505/1‑51 (MQ=255) cNGACGATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGtt > 1:5197768/1‑51 (MQ=255) cGGACGATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGtt > 1:5170635/1‑51 (MQ=255) ggACGATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTc < 1:6509822/51‑1 (MQ=255) gACGATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCa < 1:7291065/51‑1 (MQ=255) aCGATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCAt > 1:10760738/1‑51 (MQ=255) cGATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCAtt < 1:10522513/51‑1 (MQ=255) cGATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCAtt < 1:6112565/51‑1 (MQ=255) cGATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCAtt > 1:9687036/1‑51 (MQ=255) gATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCAttt < 1:8438637/51‑1 (MQ=255) gATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCAttt < 1:7783435/51‑1 (MQ=255) gATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCAttt > 1:10879107/1‑51 (MQ=255) gATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCAttt < 1:8096030/51‑1 (MQ=255) aTTGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTa > 1:8486787/1‑51 (MQ=255) aTTGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTa > 1:6480181/1‑51 (MQ=255) ttGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTAc > 1:2299604/1‑51 (MQ=255) ttGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTAc > 1:8130621/1‑51 (MQ=255) tGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTAcc > 1:9412936/1‑51 (MQ=255) tGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTAcc > 1:32116/1‑51 (MQ=255) tGCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTAcc < 1:10904287/51‑1 (MQ=255) gCCCGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCa < 1:5144940/51‑1 (MQ=255) gCCCGAGATAAATGTGTTCTGTTGGCATTCTTACCGCCAGTTCATTTACCa > 1:6536822/1‑51 (MQ=255) cccGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAg < 1:10855558/51‑1 (MQ=255) ccGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAgg > 1:503045/1‑51 (MQ=255) ccGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAgg < 1:3779506/51‑1 (MQ=255) ccGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAgg > 1:3076228/1‑51 (MQ=255) ccGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAgg < 1:7007762/51‑1 (MQ=255) ccGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAgg < 1:3183008/51‑1 (MQ=255) cGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCa > 1:685817/1‑48 (MQ=255) cGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGc > 1:10422702/1‑51 (MQ=255) cGAGATAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGc > 1:1581594/1‑51 (MQ=255) gagagaAATGTGTTCTGTTGGCATTCTGGCTGCCAGTTCa < 1:9893996/40‑1 (MQ=37) gagaTAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCt > 1:2068505/1‑51 (MQ=255) agaTAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCtt > 1:2582798/1‑51 (MQ=255) agaTAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCtt < 1:5465507/51‑1 (MQ=255) agaTAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCtt < 1:7603619/51‑1 (MQ=255) gaTAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTg > 1:9689779/1‑51 (MQ=255) gaTAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTg < 1:7594841/51‑1 (MQ=255) gaTAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTg < 1:8638556/51‑1 (MQ=255) aTAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGc > 1:1595859/1‑51 (MQ=255) aTAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGc > 1:4931556/1‑51 (MQ=255) aTAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGc > 1:3221740/1‑51 (MQ=255) aTAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGc > 1:3887086/1‑51 (MQ=255) tAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGcc > 1:7518813/1‑51 (MQ=255) tAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGcc > 1:4945320/1‑51 (MQ=255) tAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGcc > 1:10122305/1‑51 (MQ=255) tAAATGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGcc > 1:8104537/1‑51 (MQ=255) aaaTGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGc < 1:4740010/45‑1 (MQ=255) aaaTGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCg > 1:3973470/1‑51 (MQ=255) aaaTGTGTTCTGTTGGCATTCTTACTGCCAGTTCAATTACCAGGCTTGCCg > 1:2366290/1‑51 (MQ=255) aaTGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGt < 1:7911683/51‑1 (MQ=255) aaTGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGt > 1:2053874/1‑51 (MQ=255) aaTGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGt < 1:9827743/51‑1 (MQ=255) aTGTGTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTg > 1:5213986/1‑51 (MQ=255) tgtgTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGa > 1:5172924/1‑51 (MQ=255) tgtgTTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGa < 1:8248674/51‑1 (MQ=255) gtgtTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAg > 1:6137849/1‑51 (MQ=255) gtgtTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAg > 1:2437602/1‑51 (MQ=255) gtTCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAggg > 1:1112455/1‑51 (MQ=255) ttCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGt < 1:7928887/51‑1 (MQ=255) tCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTc < 1:9238176/51‑1 (MQ=255) tCTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTc < 1:4784104/51‑1 (MQ=255) cTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCg > 1:4503790/1‑51 (MQ=255) cTGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCg > 1:5392741/1‑51 (MQ=255) tGTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGa > 1:6962258/1‑51 (MQ=255) gTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGAt > 1:6046415/1‑51 (MQ=255) gTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGAt > 1:4266834/1‑51 (MQ=255) gTTGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGAt < 1:3829046/51‑1 (MQ=255) ttGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGAtt > 1:9573124/1‑51 (MQ=255) ttGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGAtt > 1:3859866/1‑51 (MQ=255) ttGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGAtt > 1:906839/1‑51 (MQ=255) ttGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGAtt > 1:1819669/1‑51 (MQ=255) ttGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGAtt > 1:9327317/1‑51 (MQ=255) ttGGCATTCTTACTGCCAATTCATTTACCAGGCTTGCCGTGAGGGTCGAtt > 1:3284694/1‑51 (MQ=255) tGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGAttt > 1:5585135/1‑51 (MQ=255) tGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGAttt < 1:10419536/51‑1 (MQ=255) tGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGAttt > 1:489772/1‑51 (MQ=255) tGGCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGAttt > 1:8006266/1‑51 (MQ=255) gCATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGATTTAc > 1:7627935/1‑51 (MQ=255) cATTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGATTTAcc < 1:5770520/51‑1 (MQ=255) aTTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGATTTACCt > 1:10013092/1‑51 (MQ=255) aTTCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGATTTACCt < 1:10249110/51‑1 (MQ=255) ttCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGATTTACCTg < 1:9613505/51‑1 (MQ=255) ttCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGATTTACCTg > 1:6439857/1‑51 (MQ=255) tCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGATTTACCTg < 1:7647688/50‑1 (MQ=255) tCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGATTTACCTGa > 1:4084602/1‑51 (MQ=255) tCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGATTTACCTGa < 1:1400330/51‑1 (MQ=255) tCTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGATTTACCTGa > 1:10566843/1‑51 (MQ=255) cTTACTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGATTTACCTGaa < 1:4054430/51‑1 (MQ=255) | ATTCGCCCGGACGATTGCCCGAGATAAATGTGTTCTGTTGGCATTCTTGCTGCCAGTTCATTTACCAGGCTTGCCGTGAGGGTCGATTTACCTGAA > REL606/3067878‑3067973 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |