| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 1,733,865 | G→T | 100% | A301S (GCC→TCC) | pykF → | pyruvate kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,733,865 | 0 | G | T | 98.4% | 268.7 / ‑3.1 | 80 | A301S (GCC→TCC) | pykF | pyruvate kinase |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (34/44); total (35/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCA > REL606/1733818‑1733912 | tCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCa > 1:6826482/1‑51 (MQ=255) cAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCAt < 1:6400361/51‑1 (MQ=255) aaaaaCCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATc < 1:9489829/51‑1 (MQ=255) aaaaaCCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATc > 1:3815594/1‑51 (MQ=255) aaacCCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATcc < 1:5657942/51‑1 (MQ=255) aaaCCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCt < 1:9716894/51‑1 (MQ=255) aaaCCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCt > 1:2247746/1‑51 (MQ=255) aaaCCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCt > 1:6287512/1‑51 (MQ=255) aaCCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTc < 1:8030950/51‑1 (MQ=255) aCCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCg > 1:5110575/1‑51 (MQ=255) cccACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATcc < 1:4295110/47‑1 (MQ=255) cccACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCg < 1:5240969/50‑1 (MQ=255) cccACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCg < 1:5826351/50‑1 (MQ=255) cACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACg > 1:607228/1‑51 (MQ=255) cACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACg > 1:1744650/1‑51 (MQ=255) cGCCTGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACgg > 1:650149/1‑50 (MQ=255) cGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGt > 1:8288424/1‑51 (MQ=255) cccGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATcc < 1:299822/41‑1 (MQ=255) cccGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTc < 1:6702808/43‑1 (MQ=255) cccGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGAc < 1:7851910/46‑1 (MQ=255) cccGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGAc < 1:929578/46‑1 (MQ=255) cccGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTAc < 1:2264026/51‑1 (MQ=255) cccGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTAc > 1:7339306/1‑51 (MQ=255) cccGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTAc > 1:8831426/1‑51 (MQ=255) ccGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACt > 1:9427028/1‑51 (MQ=255) cGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTg > 1:6960133/1‑51 (MQ=255) cGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTg < 1:5971219/51‑1 (MQ=255) gACTCGCGCAGAAGCCGGTGACGTTGTAAACTCCATCCTCGACGGTACTg < 1:342501/50‑1 (MQ=255) gACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGa > 1:2733556/1‑51 (MQ=255) gACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGa > 1:533388/1‑51 (MQ=255) gACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGa > 1:4515818/1‑51 (MQ=255) cTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTc < 1:5266819/38‑1 (MQ=255) tCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGc > 1:4384470/1‑51 (MQ=255) tCGCGCAGAAGCCCGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGc > 1:3471668/1‑51 (MQ=38) cgcgCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTg < 1:2704829/46‑1 (MQ=255) cgcgCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCa > 1:5645318/1‑51 (MQ=255) cgcAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACg < 1:5698053/38‑1 (MQ=255) cgcAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAg < 1:5561716/50‑1 (MQ=255) gcAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTg < 1:392966/51‑1 (MQ=255) aGAAGCCGGTGACGTTGCAAACTCCATCCTCGAc < 1:5512781/34‑1 (MQ=255) aGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGAt > 1:4574225/1‑51 (MQ=255) gAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGAc > 1:1127742/1‑42 (MQ=255) gAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCGGTGATg < 1:44611/51‑1 (MQ=38) gAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATg < 1:6648000/51‑1 (MQ=255) gAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATg > 1:6336905/1‑51 (MQ=255) aaGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGc > 1:6829710/1‑51 (MQ=255) aaGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGc < 1:10716874/51‑1 (MQ=255) aaGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGc > 1:2334082/1‑51 (MQ=255) aaGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGAc < 1:3412989/41‑1 (MQ=255) aGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCt < 1:4647055/51‑1 (MQ=255) aGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCt < 1:7920288/51‑1 (MQ=255) gCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTg < 1:6401127/51‑1 (MQ=255) gCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTg < 1:5157878/51‑1 (MQ=255) ccGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGt < 1:1228623/51‑1 (MQ=255) ccGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGt > 1:8269054/1‑51 (MQ=255) cGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTc > 1:2824997/1‑51 (MQ=255) cGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTc < 1:1185869/51‑1 (MQ=255) cGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTc < 1:4027422/51‑1 (MQ=255) cGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTc < 1:7905834/51‑1 (MQ=255) ggTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCt < 1:2844923/51‑1 (MQ=255) ggTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCt > 1:4524827/1‑51 (MQ=255) gTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTg < 1:3371246/51‑1 (MQ=255) gTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTg < 1:4233493/51‑1 (MQ=255) gTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTg < 1:8156808/51‑1 (MQ=255) tGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTgg > 1:405623/1‑51 (MQ=255) tGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTgg > 1:823198/1‑51 (MQ=255) tGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTgg < 1:8697669/51‑1 (MQ=255) gACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTgg < 1:10571045/50‑1 (MQ=255) cGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGa < 1:6335154/51‑1 (MQ=255) cGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGa > 1:8619886/1‑51 (MQ=255) cGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGa > 1:10797706/1‑51 (MQ=255) cGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGa > 1:792395/1‑51 (MQ=255) gTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg < 1:8588231/49‑1 (MQ=255) gTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGaa < 1:2598317/51‑1 (MQ=255) ttGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAAt > 1:9685009/1‑51 (MQ=255) tGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATc < 1:7038910/51‑1 (MQ=255) gCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATcc < 1:3264512/51‑1 (MQ=255) cAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCg < 1:9591359/51‑1 (MQ=255) cAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCg > 1:9091936/1‑51 (MQ=255) aaCTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCa > 1:3605733/1‑51 (MQ=255) | TCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCA > REL606/1733818‑1733912 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |