| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 950,280 | T→G | 100% | intergenic (+133/‑2) | lrp → / → ftsK | DNA‑binding transcriptional dual regulator, leucine‑binding/DNA‑binding membrane protein required for chromosome resolution and partitioning |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 950,280 | 0 | T | G | 95.8% | 344.9 / 3.4 | 94 | intergenic (+133/‑2) | lrp/ftsK | DNA‑binding transcriptional dual regulator, leucine‑binding/DNA‑binding membrane protein required for chromosome resolution and partitioning |
| Reads supporting (aligned to +/- strand): ref base T (4/0); new base G (36/54); total (40/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 102 total aligned reads displayed.
GTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACACTGAAGACAAAGAAGTCACATTGACAAAGTTA > REL606/950233‑950329 | gtCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCtt < 1:8211732/51‑1 (MQ=255) tCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCcttgcttg < 1:7494940/51‑1 (MQ=255) tCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCcttgcttg < 1:1532421/51‑1 (MQ=255) tCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCcttgcttg < 1:8203977/51‑1 (MQ=255) ccGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCcttgcttg > 1:8904872/1‑50 (MQ=255) ccGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGa > 1:7223454/1‑51 (MQ=255) cGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGa < 1:7016021/50‑1 (MQ=255) cGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAg < 1:4311424/51‑1 (MQ=255) cGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAg < 1:8275063/51‑1 (MQ=255) gTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGc > 1:6345755/1‑51 (MQ=255) ttttAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGcc > 1:7359329/1‑51 (MQ=255) ttttAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGcc < 1:8197012/51‑1 (MQ=255) ttttAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGcc > 1:5147063/1‑51 (MQ=255) tttAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCa > 1:5693527/1‑51 (MQ=255) tttAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCa > 1:2261468/1‑51 (MQ=255) tttAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCa < 1:8326852/51‑1 (MQ=255) tttAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCa > 1:6233808/1‑51 (MQ=255) ttAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAg > 1:9217598/1‑51 (MQ=255) ttAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAg > 1:1362611/1‑51 (MQ=255) ttAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAg < 1:2430001/51‑1 (MQ=255) tAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAgg > 1:2900950/1‑51 (MQ=255) aGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGa > 1:331241/1‑51 (MQ=255) gCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGaa > 1:4886809/1‑51 (MQ=255) cATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAAt < 1:2746836/51‑1 (MQ=255) cATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAAt > 1:6407312/1‑51 (MQ=255) cATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAAt > 1:7117742/1‑51 (MQ=255) cATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAAt < 1:2563388/51‑1 (MQ=255) atggggCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATa > 1:9607473/4‑51 (MQ=39) aTCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATa > 1:969801/1‑51 (MQ=255) aTCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATa > 1:5708003/1‑51 (MQ=255) tCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATac < 1:4331214/51‑1 (MQ=255) tCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATac < 1:6938219/51‑1 (MQ=255) cGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAAt < 1:2743553/48‑1 (MQ=255) cGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATaca > 1:8752147/1‑51 (MQ=255) gggCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATacac > 1:6984412/1‑51 (MQ=255) gggCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATacac > 1:5091955/1‑51 (MQ=255) gggCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATacac < 1:9087648/51‑1 (MQ=255) gggCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATacac < 1:785812/51‑1 (MQ=255) gggCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATacac < 1:2530290/51‑1 (MQ=255) ggCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACt < 1:3557081/51‑1 (MQ=255) ggCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACt > 1:3230470/1‑51 (MQ=255) ggCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACt < 1:8263227/51‑1 (MQ=255) gCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTg > 1:1270137/1‑51 (MQ=255) gCAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTg < 1:8222326/51‑1 (MQ=255) cAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGa < 1:894068/51‑1 (MQ=255) cAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGa < 1:2917695/51‑1 (MQ=255) cAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGa > 1:2669661/1‑51 (MQ=255) cAGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGa < 1:10008854/51‑1 (MQ=255) aGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGaa > 1:1768534/1‑51 (MQ=255) aGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGaa < 1:5074066/51‑1 (MQ=255) aGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGaa > 1:6105426/1‑51 (MQ=255) aGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGaa < 1:7395130/51‑1 (MQ=255) aGGAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGaa < 1:6621145/51‑1 (MQ=255) ggAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAg < 1:566521/51‑1 (MQ=255) ggAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAg < 1:7441407/51‑1 (MQ=255) ggAAAAGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAg < 1:66377/51‑1 (MQ=255) aaaGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACa < 1:2590768/51‑1 (MQ=255) aaaGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACa < 1:10265491/51‑1 (MQ=255) aaGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACaa < 1:5675704/51‑1 (MQ=255) aGCCTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGGAGACaaa < 1:10731549/51‑1 (MQ=39) ccTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAaaga > 1:9354280/1‑51 (MQ=255) cTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACACTGAAGACAaagca > 1:10262275/1‑49 (MQ=255) cTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAaagaa < 1:7822231/51‑1 (MQ=255) cTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAaagaa < 1:1073189/51‑1 (MQ=255) cTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAaagaa < 1:3546612/51‑1 (MQ=255) cTGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAaagaa > 1:3788703/1‑51 (MQ=255) tGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATTCACTGAAGACAaagaag < 1:6000948/51‑1 (MQ=39) tGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAaagaag < 1:6263659/51‑1 (MQ=255) tGTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAaagaag > 1:2137648/1‑51 (MQ=255) gTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGt > 1:6981551/1‑51 (MQ=255) gTAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGt > 1:7574236/1‑51 (MQ=255) tAACCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGTc < 1:9868507/51‑1 (MQ=255) aaCCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGTca < 1:8050986/51‑1 (MQ=255) aCCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGTcac < 1:3702726/51‑1 (MQ=255) aCCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGTcac > 1:2438379/1‑51 (MQ=255) aCCTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGTcac < 1:3682939/51‑1 (MQ=255) ccTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGTcaca < 1:2886511/51‑1 (MQ=255) ccTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGTcaca > 1:1473235/1‑51 (MQ=255) ccTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGTcaca < 1:10052629/51‑1 (MQ=255) cTGGAGAGCCTTTCTTGAGCCAGGAATACACTGAAGACAAAGCAGTCACAt > 1:4601571/1‑51 (MQ=255) cTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGTCACAt > 1:4825188/1‑51 (MQ=255) cTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGTCACAt < 1:7238482/51‑1 (MQ=255) cTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGTCACAt > 1:1575407/1‑51 (MQ=255) cTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGTCACAt < 1:8183424/51‑1 (MQ=255) cTGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGTCACAt > 1:4436778/1‑51 (MQ=255) tGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGTCACAtt > 1:10709043/1‑51 (MQ=255) tGGAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGTCACAtt < 1:4830650/51‑1 (MQ=255) ggAGAGCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGTCACATTg < 1:1510737/51‑1 (MQ=255) gagaGCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGTCACATTGa < 1:6051914/51‑1 (MQ=255) gagCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGTCACATTGACa < 1:9557948/51‑1 (MQ=255) gagCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGTCACATTGACa < 1:6208542/51‑1 (MQ=255) agCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGTCACATTGACaa > 1:8583597/1‑51 (MQ=255) agCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGTCACATTGACaa < 1:10111154/51‑1 (MQ=255) gCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGTCACATTGACaaa < 1:10447993/51‑1 (MQ=255) gCCTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGTCACATTGACaaa < 1:10390166/51‑1 (MQ=255) ccTTGCTTGAGCCAGGAATACACTGAAGACAAAGAAGTCACATTGACAAAg < 1:4061096/51‑1 (MQ=255) cttgcttgAGCCAGGAATACACTGAAGACAAAGAAGTCACATTGACAAAGt > 1:2772943/1‑51 (MQ=255) cttgcttgAGCCAGGAATACACTGAAGACAAAGAAGTCACATTGACAAAGt > 1:8224298/1‑51 (MQ=255) cttgcttgAGCCAGGAATACACTGAAGACAAAGAAGTCACATTGACAAAGt > 1:9969051/1‑51 (MQ=255) ttCTTGAGCCAGGAATACACTGAAGACAAAGCAGTCACATTGACAAAGTTa > 1:3004462/1‑51 (MQ=255) | GTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACACTGAAGACAAAGAAGTCACATTGACAAAGTTA > REL606/950233‑950329 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |