Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC JC	REL606	2,397,529	IS150 (–) Δ14 bp	100%	coding (1124‑1137/2646 nt)	yfcTU ←	predicted outer membrane export usher protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	2397529	2397542	14	83 [0]	[0] 143	yfcTU	predicted outer membrane export usher protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 2397528	0 (0.000)	67 (0.720)	47/78	NT	100%	coding (1138/2646 nt)	yfcTU	predicted outer membrane export usher protein
	?	REL606	2774435 =	NA (NA)					noncoding (1443/1443 nt)	IS150	repeat region
*	?	REL606	2397543 =	0 (0.000)	96 (1.070)	55/76	NT	100%	coding (1123/2646 nt)	yfcTU	predicted outer membrane export usher protein
	?	REL606	= 2775877	NA (NA)					noncoding (1/1443 nt)	IS150	repeat region

GGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGACTTTATAACGAACCTGGCCTGGGCGAG  >  REL606/2397478‑2397575                                                   |                                                ggaggCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCActc                                                 >  1:3000213/1‑51 (MQ=255)  gAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCt                                                <  1:10793936‑M1/51‑2 (MQ=255)   aGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCtg                                               <  1:6603582‑M1/51‑3 (MQ=255)   aGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCtg                                               >  1:5088670‑M1/1‑49 (MQ=255)    ggCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgt                                              >  1:2542310‑M1/1‑48 (MQ=255)    ggCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgt                                              <  1:1841845‑M1/51‑4 (MQ=255)      cTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtac                                            >  1:7514494‑M1/1‑46 (MQ=255)      cTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtac                                            >  1:9625152‑M1/1‑46 (MQ=255)      cTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtac                                            >  1:10216650‑M1/1‑46 (MQ=255)      cTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtac                                            <  1:2627770‑M1/51‑6 (MQ=255)       ttCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtact                                           >  1:2485518‑M1/1‑45 (MQ=255)       ttCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtact                                           <  1:6269662‑M1/51‑7 (MQ=255)       ttCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtact                                           <  1:10446455‑M1/51‑7 (MQ=255)        tCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtactg                                          <  1:8685487‑M1/51‑8 (MQ=255)        tCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtactg                                          <  1:7940966‑M1/51‑8 (MQ=255)         cAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtactga                                         <  1:10020961‑M1/51‑9 (MQ=255)          aGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtactgac                                        >  1:9672716‑M1/1‑42 (MQ=255)            cACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtactgaccc                                      <  1:6851666‑M1/51‑12 (MQ=255)            cACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtactgaccc                                      >  1:1488407‑M1/1‑40 (MQ=255)            cACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtactgaccc                                      >  1:7472001‑M1/1‑40 (MQ=255)             aCCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtactgacccc                                     >  1:6839150‑M1/1‑39 (MQ=255)             aCCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtactgacccc                                     >  1:5451778‑M1/1‑39 (MQ=255)              ccAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtactgacccca                                    <  1:4161441‑M1/51‑14 (MQ=255)              ccAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtactgacccca                                    >  1:10173401‑M1/1‑38 (MQ=255)              ccAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtactgacccca                                    <  1:8944942‑M1/51‑14 (MQ=255)               cAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtactgaccccaa                                   <  1:3665930‑M1/51‑15 (MQ=255)                agagAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtactgaccccaaa                                  <  1:3916039‑M1/51‑16 (MQ=255)                agagAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtactgaccccaaa                                  <  1:6609783‑M1/51‑16 (MQ=255)                agagAAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtactgaccccaaa                                  <  1:5779631‑M1/51‑16 (MQ=255)                 gagaAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtactgaccccaaaa                                 <  1:9832033‑M1/51‑17 (MQ=255)                 gagaAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtactgaccccaaaa                                 >  1:2764003‑M1/1‑35 (MQ=255)                  agaAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtactgaccccaaaaa                                <  1:6632142‑M1/51‑18 (MQ=255)                  agaAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtactgaccccaaaaa                                >  1:2096668‑M1/1‑34 (MQ=255)                  agaAAAATCCCCCCTCGACATGGTGGCCCCACTCtgtactgaccccaaaaa                                <  1:7429559‑M1/51‑18 (MQ=255)                    aaaaaTCCCCCCTCGACATGGTGGCCCCACTCtgtactgaccccaaaaagt                              >  1:1382050‑M1/1‑32 (MQ=255)                    aaaaaTCCCCCCTCGACATGGTGGCCCCACTCtgtactgaccccaaaaagt                              >  1:396857‑M1/1‑32 (MQ=255)                    aaaaaTCCCCCCTCGACATGGTGGCCCCACTCtgtactgaccccaaaaagt                              <  1:7413489‑M1/51‑20 (MQ=255)                     aaaaTCCCCCCTCGACATGGTGGCCCCACTCtgtactgaccccaaaaagtt                             >  1:3800926‑M1/1‑31 (MQ=255)                     aaaaTCCCCCCTCGACATGGTGGCCCCACTCtgtactgaccccaaaaagtt                             >  1:1565489‑M1/1‑31 (MQ=255)                       aaTCCCCCCTCGACATGGTGGCCCCACTCtgtactgaccccaaaaagttgg                           >  1:10618300‑M1/1‑29 (MQ=255)                       aaTCCCCCCTCGACATGGTGGCCCCACTCtgtactgaccccaaaaagttgg                           >  1:9125063‑M1/1‑29 (MQ=255)                       aaTCCCCCCTCGACATGGTGGCCCCACTCtgtactgaccccaaaaagttgg                           >  1:10746437‑M1/1‑29 (MQ=255)                       aaTCCCCCCTCGACATGGTGGCCCCACTCtgtactgaccccaaaaagttgg                           <  1:2700337‑M1/51‑23 (MQ=255)                        aTCCCCCCTCGACATGGTGGCCCCACTCtgtactgaccccaaaaagttgga                          >  1:710027‑M1/1‑28 (MQ=255)                          ccccccTCGACATGGTGGCCCCACTCtgtactgaccccaaaaagttggaca                        >  1:4710494‑M1/1‑26 (MQ=255)                          ccccccTCGACATGGTGGCCCCACTCtgtactgaccccaaaaagttggaca                        >  1:5772451‑M1/1‑26 (MQ=255)                          ccccccTCGACATGGTGGCCCCACTCtgtactgaccccaaaaagttggac                         <  1:811240‑M1/50‑25 (MQ=255)                             cccTCGACATGGTGGCCCCACTCtgtactgaccccaaaaagttggacagtt                     >  1:6583840‑M1/1‑23 (MQ=255)                             cccTCGACATGGTGGCCCCACTCtgtactgaccccaaaaagtt                             <  1:519540‑M1/43‑21 (MQ=255)                              ccTCGACATGGTGGCCCCACTCtgtactgaccccaaaagttggacagttaa                    <  1:1263470‑M1/51‑30 (MQ=255)                              ccTCGACATGGTGGCCCCACTCtgtactgaccccaaaaagttggacagtta                    <  1:2609976‑M1/51‑30 (MQ=255)                              ccTCGACATGGTGGCCCCACTCtgtactgaccccaaaaagttggacagtta                    <  1:4455531‑M1/51‑30 (MQ=255)                              ccTCGACATGGTGGCCCCACTCtgtactgaccccaaaaagttggacagtta                    >  1:3262745‑M1/1‑22 (MQ=255)                               cTCGACATGGTGGCCCCACTCtgtactgaccccaaaaagttggacagttaa                   <  1:1885329‑M1/51‑31 (MQ=255)                               cTCGACATGGTGGCCCCACTCtgtactgaccccaaaaagttggacagttaa                   <  1:3011246‑M1/51‑31 (MQ=255)                               cTCGACATGGTGGCCCCACTCtgtactgaccccaaaaagttggacagttaa                   <  1:3468992‑M1/51‑31 (MQ=255)                                tCGACATGGTGGCCCCACTCtgtactgaccccaaaaagttggacagttaaa                  <  1:5261948‑M1/51‑32 (MQ=255)                                tCGACATGGTGGCCCCACTCtgtactgaccccaaaaagttggacagttaaa                  >  1:4730869‑M1/1‑20 (MQ=255)                                tCGACATGGTGGCCCCACTCtgtactgaccccaaaaagttggacagttaaa                  <  1:9320097‑M1/51‑32 (MQ=255)                                 cGACATGGTGGCCCCACTCtgtactgaccccaaaaagttggacagttaaac                 <  1:6697409‑M1/51‑33 (MQ=255)                                 cGACATGGTGGCCCCACTCtgtactgaccccaaaaagttggacagttaaac                 >  1:8400661‑M1/1‑19 (MQ=255)                                 cGACATGGTGGCCCCACTCtgtactgaccccaaaaagttggacagttaaac                 <  1:7227021‑M1/51‑33 (MQ=255)                                 cGACATGGTGGCCCCACTCtgtactgaccccaaaaagttggacagttaaa                  >  1:3813920‑M1/1‑19 (MQ=255)                                  gACATGGTGGCCCCACTCtgtactgaccccaaaaagttggacagttaaaca                <  1:10290071‑M1/51‑34 (MQ=255)                                  gACATGGTGGCCCCACTCtgtactgaccccaaaaagttggacagttaaaca                <  1:2296283‑M1/51‑34 (MQ=255)                                  gACATGGTGGCCCCACTCtgtactgaccccaaaaagttggacagttaaaca                >  1:1084294‑M1/1‑18 (MQ=255)                                   aCATGGTGGCCCCACTCtgtactgaccccaaaaagttggacagttaaacac               <  1:2852399‑M1/51‑35 (MQ=255)                                   aCATGGTGGCCCCACTCtgtactgaccccaaaaagttggacagttaaacac               >  1:8665534‑M1/1‑17 (MQ=255)                                    cATGGTGGCCCCACTCtgtactgaccccaaaaagttggacagttaa                   >  1:10406660‑M1/1‑16 (MQ=255)                                     aTGGTGGCCCCACTCtgtactgaccccaaaaagttggacagttaaacacga             <  1:6421881‑M1/51‑37 (MQ=255)                                     aTGGTGGCCCCACTCtgtactgaccccaaaaagttggacagttaaacacga             >  1:967155‑M1/1‑15 (MQ=255)                                       ggtggCCCCACTCtgtactgaccccaaaaagttggacagttaaacacgagg           <  1:5877883‑M1/51‑39 (MQ=255)                                        gtggCCCCACTCtgtactgaccccaaaaagttggacagttaaacacgaggc          <  1:3720266‑M1/51‑40 (MQ=255)                                         tggCCCCACTCtgtactgaccccaaaaagttggacagttaaacacgaggca         <  1:9266849‑M1/51‑41 (MQ=255)                                          ggCCCCACTCtgtactgaccccaaaaagttggacagttaaacacgaggcat        >  1:9688659‑M1/1‑10 (MQ=255)                                          ggCCCCACTCtgtactgaccccaaaaagttggacagttaaacacgaggcat        <  1:7574829‑M1/51‑42 (MQ=255)                                           gCCCCACTCtgtactgaccccaaaaagttggacagttaaacacgaggcata       <  1:3464693‑M1/51‑43 (MQ=255)                                             cccACTCtgtactgaccccaaaaagttggacagttaaacacgaggcatata     <  1:3611424‑M1/51‑45 (MQ=255)                                             cccACTCtgtactgaccccaaaaagttggacagttaaacacgaggcatata     >  1:4887339‑M1/1‑7 (MQ=255)                                              ccACTCtgtactgaccccaaaaagttggacagttaaacacgaggcatatag    >  1:1775377‑M1/1‑6 (MQ=255)                                               cACTCtgtactgaccccaaaaagttggacagttaaacacgaggcatatagg   <  1:2877587‑M1/51‑47 (MQ=255)                                                aCTCtgtactgaccccaaaaagttggacagttaaacacgaggcatataggt  <  1:8156729‑M1/51‑48 (MQ=255)                                                aCTCtgtactgaccccaaaaagttggacagttaaacacgaggcatataggt  >  1:7420135‑M1/1‑4 (MQ=255)                                                   |                                                GGAGGCTTCAGCACCAGAGAAAAATCCCCCCTCGACATGGTGGCCCCACTCTTGCGGACGTCCCATCATGACTTTATAACGAACCTGGCCTGGGCGAG  >  REL606/2397478‑2397575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑