Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC REL606 3,894,997 Δ6,138 bp 100% IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 3893584–3894993 3901134 6142–7551 56 [51] [0] 227 insJ‑5–[yieO] insJ‑5,insK‑5,rbsD,rbsA,rbsC,rbsB,rbsK,rbsR,[yieO]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 3894996NA (NA)133 (1.440) 64/78 NT 100% noncoding (1443/1443 nt) IS150 repeat region
?REL606 3901135 = 0 (0.000)coding (1285/1428 nt) yieO predicted multidrug or homocysteine efflux system

GACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGAC  >  REL606/3894949‑3894999
                                               |   
gACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGAc  <  1:1768627/51‑1 (MQ=35)
                                               |   
GACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGAC  >  REL606/3894949‑3894999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.