Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,201,980	G→A	P194S (CCT→TCT)	iclR ←	DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,201,980	0	G	A	100.0%	333.4 / NA	94	P194S (CCT→TCT)	iclR	DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (46/48);  total (46/48)

AATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTGCA  >  REL606/4201932‑4202027                                                 |                                                aaTAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGaag                                               >  1:3166476/1‑51 (MQ=255)  aTAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACagaaga                                              >  1:6562301/1‑51 (MQ=255)  aTAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACagaaga                                              >  1:6625827/1‑51 (MQ=255)  aTAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACagaaga                                              >  1:5302498/1‑51 (MQ=255)  aTAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACagaaga                                              <  1:6556154/51‑1 (MQ=255)   tAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGac                                             <  1:6939603/51‑1 (MQ=255)    aaCCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGaca                                            <  1:3388773/51‑1 (MQ=255)    aaCCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGaca                                            >  1:3753640/1‑51 (MQ=255)     aCCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGacac                                           >  1:4734131/1‑51 (MQ=255)      ccccGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGacac                                           <  1:1811116/50‑1 (MQ=255)       cccGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCa                                         <  1:5011222/51‑1 (MQ=255)       cccGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCa                                         <  1:6563086/51‑1 (MQ=255)        ccGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAg                                        <  1:5647037/51‑1 (MQ=255)         cGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGc                                       <  1:4123481/51‑1 (MQ=255)         cGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGc                                       >  1:4084290/1‑51 (MQ=255)         cGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGc                                       >  1:6435791/1‑51 (MQ=255)          gTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCg                                      >  1:5551942/1‑51 (MQ=255)          gTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCg                                      <  1:4539203/51‑1 (MQ=255)          gTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCg                                      >  1:2840498/1‑51 (MQ=255)          gTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCg                                      <  1:6522787/51‑1 (MQ=255)          gTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCg                                      <  1:2962641/51‑1 (MQ=255)          gTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCg                                      >  1:3398041/1‑51 (MQ=255)          gTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCg                                      >  1:808212/1‑51 (MQ=255)           tttGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGt                                     >  1:6357533/1‑51 (MQ=255)            ttGCGCGTTTGGGCGAGATCTTCTTTTAAATGCGCAGAAGACAcc                                          <  1:1462397/45‑1 (MQ=255)            ttGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGtt                                    >  1:4456639/1‑51 (MQ=255)            ttGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGtt                                    <  1:3050155/51‑1 (MQ=255)            ttGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGtt                                    <  1:3093772/51‑1 (MQ=255)            ttGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGtt                                    >  1:6740684/1‑51 (MQ=255)             tGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTg                                   <  1:4112941/51‑1 (MQ=255)             tGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTg                                   >  1:6954023/1‑51 (MQ=255)             tGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTg                                   >  1:1777281/1‑51 (MQ=255)              gcgcGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGc                                  <  1:1001617/51‑1 (MQ=255)              gcgcGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGc                                  <  1:2975373/51‑1 (MQ=255)              gcgcGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGc                                  <  1:1502841/51‑1 (MQ=255)               cgcgTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCg                                 <  1:6837180/51‑1 (MQ=255)               cgcgTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCg                                 >  1:2817090/1‑51 (MQ=255)                 cgTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTg                               >  1:690085/1‑51 (MQ=255)                  gTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTgg                              <  1:6757872/51‑1 (MQ=255)                  gTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTgg                              >  1:792308/1‑51 (MQ=255)                  gTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTgg                              <  1:2424717/51‑1 (MQ=255)                  gTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTgg                              <  1:5310867/51‑1 (MQ=255)                   tttGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTggg                             >  1:295983/1‑51 (MQ=255)                   tttGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTggg                             >  1:1380359/1‑51 (MQ=255)                   tttGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTggg                             >  1:4521796/1‑51 (MQ=255)                   tttGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTggg                             >  1:1614281/1‑51 (MQ=255)                    ttGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTggg                             >  1:631781/1‑50 (MQ=255)                    ttGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGt                            >  1:583129/1‑51 (MQ=255)                    ttGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGt                            <  1:4369985/51‑1 (MQ=255)                     tGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGta                           <  1:99428/51‑1 (MQ=255)                     tGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGta                           >  1:738965/1‑51 (MQ=255)                     tGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGta                           <  1:358086/51‑1 (MQ=255)                      gggCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGtat                          >  1:1154902/1‑51 (MQ=255)                       ggCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGtata                         <  1:2875611/51‑1 (MQ=255)                        gCGAGATCTTCTTTTAAATGCACAGAAGACTCCAGCGTTGCGTGGGTATAg                        <  1:791021/51‑1 (MQ=255)                        gCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAg                        >  1:601912/1‑51 (MQ=255)                          gagaTCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGc                      >  1:1347588/1‑51 (MQ=255)                           agaTCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCa                     >  1:2727330/1‑51 (MQ=255)                            gaTCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCAt                    >  1:2888976/1‑51 (MQ=255)                             aTCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATg                   >  1:6775666/1‑51 (MQ=255)                             aTCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATg                   <  1:1855231/51‑1 (MQ=255)                               cttcttTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAgg                       <  1:438215/45‑1 (MQ=255)                               cttcttTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAgg                       <  1:6831496/45‑1 (MQ=255)                               cttcttTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGc                      <  1:2298454/46‑1 (MQ=255)                               cttcttTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTa                 <  1:7039270/51‑1 (MQ=255)                                ttcttTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTaa                >  1:4653141/1‑51 (MQ=255)                                ttcttTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTaa                >  1:166771/1‑51 (MQ=255)                                 tcttTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAAc               >  1:5306649/1‑51 (MQ=255)                                 tcttTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAAc               >  1:3031770/1‑51 (MQ=255)                                 tcttTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAAc               >  1:5735746/1‑51 (MQ=255)                                  cttTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAAcc              <  1:5130258/51‑1 (MQ=255)                                  cttTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAAcc              <  1:22007/51‑1 (MQ=255)                                   ttttAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAAccc             <  1:3271811/51‑1 (MQ=255)                                    tttAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCt            <  1:765750/51‑1 (MQ=255)                                    tttAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCt            <  1:1722667/51‑1 (MQ=255)                                    tttAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCt            <  1:4624951/51‑1 (MQ=255)                                    tttAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCt            >  1:3444204/1‑51 (MQ=255)                                    tttAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCt            >  1:5980243/1‑51 (MQ=255)                                     ttAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCtt           >  1:1576457/1‑51 (MQ=255)                                      tAAATGCACAGAAGACACCAGCGTTGCGTTGGTATAGGCATGTAACCCttt          >  1:1457491/1‑51 (MQ=255)                                      tAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCttt          <  1:2575863/51‑1 (MQ=255)                                      tAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCttt          <  1:711491/51‑1 (MQ=255)                                      tAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCttt          >  1:1807870/1‑51 (MQ=255)                                      tAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCttt          >  1:886722/1‑51 (MQ=255)                                       aaaTGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTg         <  1:3278984/51‑1 (MQ=255)                                       aaaTGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTg         <  1:6661781/51‑1 (MQ=255)                                       aaaTGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTg         >  1:1773871/1‑51 (MQ=255)                                        aaTGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGc        <  1:4338996/51‑1 (MQ=255)                                         aTGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCg       <  1:4367293/51‑1 (MQ=255)                                         aTGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCg       <  1:6247193/51‑1 (MQ=255)                                         aTGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCg       <  1:1989564/51‑1 (MQ=255)                                         aTGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCg       <  1:5281166/51‑1 (MQ=255)                                         aTGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCg       >  1:5222146/1‑51 (MQ=255)                                           gCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGt     <  1:296353/51‑1 (MQ=255)                                           gCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGt     >  1:4718745/1‑51 (MQ=255)                                           gCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGt     <  1:61709/51‑1 (MQ=255)                                            cacaGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTg    >  1:4770098/1‑51 (MQ=255)                                            cacaGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTg    >  1:2822307/1‑51 (MQ=255)                                              caGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTgca  >  1:1897907/1‑51 (MQ=255)                                                 |                                                AATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTGCA  >  REL606/4201932‑4202027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑