Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	393,720	G→A	R96H (CGC→CAC)	queA →	S‑adenosylmethionine:tRNA ribosyltransferase‑isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	393,720	0	G	A	100.0%	244.1 / NA	72	R96H (CGC→CAC)	queA	S‑adenosylmethionine:tRNA ribosyltransferase‑isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/36);  total (36/36)

TGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCGCGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATG  >  REL606/393672‑393767                                                 |                                                tGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTcacg                                               <  1:5372534/51‑1 (MQ=255)    tGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCt                                            >  1:2386146/1‑51 (MQ=255)    tGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCt                                            <  1:1215563/51‑1 (MQ=255)    tGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCt                                            <  1:6358943/51‑1 (MQ=255)    tGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCt                                            >  1:6966505/1‑51 (MQ=255)     ggTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTc                                           >  1:108122/1‑51 (MQ=255)     ggTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTc                                           <  1:1638780/51‑1 (MQ=255)      gTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCg                                          >  1:5131018/1‑51 (MQ=255)       ttGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGa                                         >  1:6654100/1‑51 (MQ=255)       ttGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGa                                         <  1:3872409/51‑1 (MQ=255)          aaCGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAg                                      >  1:7134625/1‑51 (MQ=255)          aaCGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAg                                      >  1:4034639/1‑51 (MQ=255)           aCGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGaaa                                       >  1:6985590/1‑49 (MQ=255)           aCGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAgc                                     <  1:6922880/51‑1 (MQ=255)           aCGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAgc                                     >  1:6683472/1‑51 (MQ=255)            cGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGaaa                                       >  1:646739/1‑48 (MQ=255)            cGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAgcg                                    <  1:4386676/51‑1 (MQ=255)            cGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAgcg                                    <  1:2109235/51‑1 (MQ=255)              gATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGcc                                  >  1:3359490/1‑51 (MQ=255)               aTGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCa                                 >  1:6790284/1‑51 (MQ=255)               aTGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCa                                 <  1:1987669/51‑1 (MQ=255)               aTGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCa                                 >  1:1280213/1‑51 (MQ=255)               aTGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCa                                 <  1:2268748/51‑1 (MQ=255)                tGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCaa                                >  1:3174635/1‑51 (MQ=255)                 gCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCaaa                               >  1:5162498/1‑51 (MQ=255)                 gCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCaaa                               >  1:7140109/1‑51 (MQ=255)                  cTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCaaaa                              <  1:4346962/51‑1 (MQ=255)                   tCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAAc                             >  1:882197/1‑51 (MQ=255)                   tCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAAc                             <  1:3036359/51‑1 (MQ=255)                     gacgacAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCt                           <  1:6256485/51‑1 (MQ=255)                      acgacAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTg                          <  1:6469794/51‑1 (MQ=255)                      acgacAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTg                          >  1:6812464/1‑51 (MQ=255)                      acgacAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTg                          <  1:5456567/51‑1 (MQ=255)                       cgacAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTgg                         <  1:1643220/51‑1 (MQ=255)                         acAACCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCaaaa                              <  1:7024638/44‑1 (MQ=37)                         acAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGgcg                       >  1:4512420/1‑51 (MQ=255)                         acAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGgc                        >  1:1702427/1‑50 (MQ=255)                           aaaCGCATTCTTGCGCATATTCACGCCTCGaa                                        <  1:872991/32‑1 (MQ=255)                           aaaCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCa                     <  1:2728688/51‑1 (MQ=255)                           aaaCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCa                     <  1:1359738/51‑1 (MQ=255)                           aaaCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCa                     <  1:6280756/51‑1 (MQ=255)                           aaaCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCa                     >  1:3724783/1‑51 (MQ=255)                           aaaCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCa                     <  1:3853227/51‑1 (MQ=255)                           aaaCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCa                     <  1:2078780/51‑1 (MQ=255)                           aaaCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCa                     >  1:3083177/1‑51 (MQ=255)                            aaCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAg                    <  1:520633/51‑1 (MQ=255)                            aaCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAg                    >  1:3516809/1‑51 (MQ=255)                             aCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCt                           <  1:3208746/43‑1 (MQ=255)                               gCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGaa                  <  1:5340677/50‑1 (MQ=255)                               gCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAAc                 <  1:5712046/51‑1 (MQ=255)                                cATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAAct                <  1:145185/51‑1 (MQ=255)                                 aTTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActg               >  1:4182058/1‑51 (MQ=255)                                 aTTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActg               >  1:7212963/1‑51 (MQ=255)                                 aTTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActg               <  1:6250960/51‑1 (MQ=255)                                  ttCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgc              >  1:5531121/1‑51 (MQ=255)                                  ttCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgc              >  1:1673478/1‑51 (MQ=255)                                  ttCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgc              >  1:1098486/1‑51 (MQ=255)                                   tCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgct             >  1:1891404/1‑51 (MQ=255)                                   tCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgct             <  1:6875907/51‑1 (MQ=255)                                   tCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgct             >  1:6345029/1‑51 (MQ=255)                                     ttGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgctgc           <  1:2576222/51‑1 (MQ=255)                                      tGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgctgct          <  1:4080024/51‑1 (MQ=255)                                      tGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgctgct          >  1:2400546/1‑51 (MQ=255)                                       gcgcATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgctgctg         >  1:7299205/1‑51 (MQ=255)                                       gcgcATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgctgctg         <  1:585298/51‑1 (MQ=255)                                       gcgcATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgct             <  1:1309641/47‑1 (MQ=255)                                         gcATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTggg       >  1:4525147/1‑51 (MQ=255)                                            tatTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGa    <  1:6082313/51‑1 (MQ=255)                                             atTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGAt   <  1:3982518/51‑1 (MQ=255)                                             atTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGAt   >  1:6102441/1‑51 (MQ=255)                                              ttCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATg  <  1:159678/51‑1 (MQ=255)                                              ttCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATg  >  1:6293182/1‑51 (MQ=255)                                              ttCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATg  >  1:2351426/1‑51 (MQ=255)                                                 |                                                TGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCGCGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATG  >  REL606/393672‑393767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑