| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,409,316 | T→G | S41S (TCT→TCG) | yheS → | fused predicted transporter subunits of ABC superfamily: ATP‑binding components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,409,316 | 0 | T | G | 98.7% | 292.2 / ‑4.1 | 78 | S41S (TCT→TCG) | yheS | fused predicted transporter subunits of ABC superfamily: ATP‑binding components |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (28/49); total (29/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01 | |||||||||||
CCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCTACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAG > REL606/3409268‑3409363 | ccccGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGAc < 1:595826/51‑1 (MQ=255) ccGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGAccc > 1:6228298/1‑51 (MQ=255) cGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCct > 1:4920024/1‑51 (MQ=255) cGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCct < 1:7121797/51‑1 (MQ=255) cGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCct > 1:2195178/1‑51 (MQ=255) cGGGCAGAAAGTCGGCCTGGGGGGTAAAAACGGCTGTGGTAAATCGAccc > 1:4597331/1‑50 (MQ=39) gggCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCctg < 1:3926404/51‑1 (MQ=255) gggCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCctg > 1:2038028/1‑51 (MQ=255) gggCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCctg > 1:2341384/1‑51 (MQ=255) ggCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCctgc < 1:5615653/51‑1 (MQ=255) gCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCctgct < 1:6908931/51‑1 (MQ=255) gCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCctgct < 1:5493483/51‑1 (MQ=255) gCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCctgct > 1:6701251/1‑51 (MQ=255) gCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCctgct < 1:4555637/51‑1 (MQ=255) gCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCctgct < 1:3152075/51‑1 (MQ=255) gCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCctgct < 1:6517557/51‑1 (MQ=255) cAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCctgctg < 1:4671442/51‑1 (MQ=255) ggAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTgg < 1:1501041/50‑1 (MQ=255) aaGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCAt < 1:4508624/51‑1 (MQ=255) aaGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCAt < 1:255296/51‑1 (MQ=255) aGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCAtt < 1:4486582/51‑1 (MQ=255) aGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCAtt > 1:4916078/1‑51 (MQ=255) aGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCAtt < 1:2942861/51‑1 (MQ=255) gTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTg < 1:2778469/51‑1 (MQ=255) gTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTg < 1:4845318/51‑1 (MQ=255) tCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGc < 1:5089127/51‑1 (MQ=255) ggCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTg > 1:6439318/1‑51 (MQ=255) gCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGa < 1:6354502/51‑1 (MQ=255) gCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGa > 1:3775654/1‑51 (MQ=255) ccTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGaa < 1:3705676/51‑1 (MQ=255) ccTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGaa < 1:4784976/51‑1 (MQ=255) tggtggGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGaaaa > 1:4614945/1‑51 (MQ=255) ggtggGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGaaaaa < 1:4835409/51‑1 (MQ=255) ggtggGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGaaaaa < 1:758907/51‑1 (MQ=255) gtggGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAAt > 1:5718224/1‑51 (MQ=255) tggGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATg < 1:1138442/51‑1 (MQ=255) tggGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATg > 1:5602824/1‑51 (MQ=255) ggTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGaa < 1:4213635/51‑1 (MQ=255) ggTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGaa < 1:1895688/51‑1 (MQ=255) tAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAAt > 1:5314204/1‑51 (MQ=255) tAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAAt < 1:1268114/51‑1 (MQ=255) tAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAAt < 1:5717454/51‑1 (MQ=255) aaaaaCGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATc > 1:4153723/1‑51 (MQ=255) aaaaaCGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATc < 1:2218681/51‑1 (MQ=255) aaaaCGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCa > 1:2635445/1‑51 (MQ=255) aaaaCGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCa < 1:4711421/51‑1 (MQ=255) aaaaCGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCa < 1:3982049/51‑1 (MQ=255) aaaCGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAg < 1:5126852/51‑1 (MQ=255) aaCGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAgc > 1:2232222/1‑51 (MQ=255) aaCGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAgc > 1:2212508/1‑51 (MQ=255) aCGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAgcg < 1:3119328/51‑1 (MQ=255) aCGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAgcg < 1:2535837/51‑1 (MQ=255) aCGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAgcg < 1:3114149/51‑1 (MQ=255) cGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAgcgc > 1:974054/1‑51 (MQ=255) cGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAgcgc > 1:1315411/1‑51 (MQ=255) cGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAgcgc < 1:239929/51‑1 (MQ=255) ggCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGcc < 1:2663185/51‑1 (MQ=255) ggCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGcc < 1:2587435/51‑1 (MQ=255) gCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAgc > 1:3650061/1‑47 (MQ=255) gCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCg < 1:5712976/51‑1 (MQ=255) gCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCg < 1:3378528/51‑1 (MQ=255) gCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCg < 1:5995554/51‑1 (MQ=255) gCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCg > 1:1773987/1‑51 (MQ=255) cTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGa > 1:1600889/1‑51 (MQ=255) cTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGa < 1:4304101/51‑1 (MQ=255) cTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGa < 1:3981403/51‑1 (MQ=255) tgtgGTAAATCTACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGAc > 1:666067/1‑51 (MQ=255) tgtgGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGAc > 1:2918993/1‑51 (MQ=255) gtgGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGAcg > 1:5967297/1‑51 (MQ=255) gtgGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGAcg < 1:72098/51‑1 (MQ=255) tgGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGAcgg < 1:2456047/51‑1 (MQ=255) gTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGAcggcg < 1:4585280/51‑1 (MQ=255) gTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGAcggcg < 1:6392709/51‑1 (MQ=255) tAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGAcggcgg > 1:6318965/1‑51 (MQ=255) tAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGAcggcgg < 1:3572918/51‑1 (MQ=255) aaTCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCa > 1:5020505/1‑51 (MQ=255) aaTCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCa < 1:4162884/51‑1 (MQ=255) aTCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAg > 1:3138625/1‑51 (MQ=255) | CCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCTACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAG > REL606/3409268‑3409363 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |