Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,287,483	T→C	M260V (ATG→GTG)	arcB ←	hybrid sensory histidine kinase in two‑component regulatory system with ArcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,287,483	0	T	C	100.0%	218.2 / NA	57	M260V (ATG→GTG)	arcB	hybrid sensory histidine kinase in two‑component regulatory system with ArcA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/29);  total (28/29)

GCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACAAA  >  REL606/3287435‑3287530                                                 |                                                gcgcATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCccacca                                               <  1:2995531/51‑1 (MQ=255) gcgcATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCccacca                                               <  1:5975100/51‑1 (MQ=255)  cgcATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCaa                                              >  1:231690/1‑51 (MQ=255)  cgcATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCaa                                              >  1:6750613/1‑51 (MQ=255)   gcATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCaaa                                             <  1:5411467/51‑1 (MQ=255)    cATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAAc                                            >  1:4333032/1‑51 (MQ=255)    cATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAAc                                            >  1:1725685/1‑51 (MQ=255)    cATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAAc                                            >  1:7013469/1‑51 (MQ=255)     aTCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAAcc                                           >  1:6881279/1‑51 (MQ=255)     aTCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAAcc                                           <  1:4313135/51‑1 (MQ=255)     aTCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAAcc                                           <  1:2449567/51‑1 (MQ=255)     aTCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAAcc                                           >  1:3838962/1‑51 (MQ=255)     aTCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAAcc                                           >  1:294030/1‑51 (MQ=255)      tCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCg                                          >  1:1914048/1‑51 (MQ=255)      tCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCg                                          >  1:6796599/1‑51 (MQ=255)       ccTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGt                                         <  1:652298/51‑1 (MQ=255)         tGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGa                                       >  1:6596073/1‑51 (MQ=255)          gATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGAc                                      <  1:6731431/51‑1 (MQ=255)          gATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGAc                                      <  1:2931851/51‑1 (MQ=255)          gATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGAc                                      <  1:6304721/51‑1 (MQ=255)           aTACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACg                                     >  1:7280109/1‑51 (MQ=255)            tACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGt                                    >  1:1344567/1‑51 (MQ=255)            tACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGt                                    >  1:5891257/1‑51 (MQ=255)             aCCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGtt                                   <  1:3990846/51‑1 (MQ=255)              ccGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGttt                                  <  1:6051479/51‑1 (MQ=255)              ccGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGttt                                  <  1:1295574/51‑1 (MQ=255)                gCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTAc                                <  1:4816427/51‑1 (MQ=255)                gCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTAc                                <  1:6445818/51‑1 (MQ=255)                 cTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTAcc                               <  1:5242057/51‑1 (MQ=255)                 cTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTAcc                               <  1:7190682/51‑1 (MQ=255)                  ttACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTAccc                              >  1:904575/1‑51 (MQ=255)                     cGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCAcg                           <  1:69298/51‑1 (MQ=255)                     cGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCAcg                           >  1:4222753/1‑51 (MQ=255)                       cTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCAcgcg                         >  1:4130857/1‑51 (MQ=255)                       cTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCAcgcg                         <  1:2930000/51‑1 (MQ=255)                       cTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCAcgcg                         >  1:6468598/1‑51 (MQ=255)                       cTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCAcgcg                         <  1:3089318/51‑1 (MQ=255)                        tCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCgg                        <  1:203770/51‑1 (MQ=255)                          ggTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTc                      >  1:3738181/1‑51 (MQ=255)                          ggTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTc                      <  1:5204313/51‑1 (MQ=255)                          ggTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTc                      >  1:2230247/1‑51 (MQ=255)                          ggTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTc                      >  1:225664/1‑51 (MQ=255)                            tAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCgt                    <  1:3891858/51‑1 (MQ=255)                             aaTGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCgta                   <  1:6322711/51‑1 (MQ=255)                             aaTGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCgta                   <  1:5538405/51‑1 (MQ=255)                             aaTGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCgta                   >  1:6969671/1‑51 (MQ=255)                               tGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCgtagt                 >  1:2870670/1‑51 (MQ=255)                                gTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCgtagta                <  1:6537144/51‑1 (MQ=255)                                gTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCgtagta                <  1:3938308/51‑1 (MQ=255)                                 tCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTAc               <  1:2963449/51‑1 (MQ=255)                                 tCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTAc               <  1:3505630/51‑1 (MQ=255)                                    cgACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGc            >  1:2869557/1‑51 (MQ=255)                                    cgACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGc            >  1:6037214/1‑51 (MQ=255)                                     gACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCa           >  1:876970/1‑51 (MQ=255)                                     gACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCa           >  1:7263057/1‑51 (MQ=255)                                     gACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCa           >  1:2117806/1‑51 (MQ=255)                                      aCCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCAc          <  1:1007060/51‑1 (MQ=255)                                          aaGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTa      <  1:7112787/51‑1 (MQ=255)                                             cccACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACaa   <  1:3262272/51‑1 (MQ=255)                                              ccaccaAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACaaa  <  1:614261/51‑1 (MQ=255)                                                 |                                                GCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACAAA  >  REL606/3287435‑3287530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑