Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,343,489	G→A	L542L (CTG→TTG)	yciR ←	modulator of Rnase II stability

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,343,489	0	G	A	100.0%	211.9 / NA	61	L542L (CTG→TTG)	yciR	modulator of Rnase II stability
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/22);  total (39/22)

GTACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAG  >  REL606/1343441‑1343536                                                 |                                                gTACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAAtt                                               >  1:6099498/1‑51 (MQ=255) gTACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAAtt                                               <  1:298595/51‑1 (MQ=255)  tACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAATtc                                              >  1:816375/1‑51 (MQ=255)    cTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAATtcat                                            <  1:1246653/51‑1 (MQ=255)    cTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAATtcat                                            <  1:566467/51‑1 (MQ=255)     ttGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAATtcatc                                           >  1:3333789/1‑51 (MQ=255)     ttGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAATtcatc                                           >  1:5459221/1‑51 (MQ=255)         gcACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAATTCATCATtc                                       >  1:2807764/1‑51 (MQ=255)          cACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAATTCATCATtct                                      >  1:977417/1‑51 (MQ=255)             cTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAATTCATCATTCTCaa                                   <  1:7097184/51‑1 (MQ=255)              tAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAATTCATCATTCTCAAt                                  <  1:7277337/51‑1 (MQ=255)               aGTTGGCTAAATTGTTGAATAACAGACAGTGCCAATTCATCATTCTCAATc                                 <  1:5179705/51‑1 (MQ=255)                gTTGGCTAAATTGTTGAATAACAGACAGTGCCAATTCATCATTCTCAATCa                                >  1:2901511/1‑51 (MQ=255)                 ttGGCTAAATTGTTGAATAACAGACAGTGCCAATTCATCATTCTCAATCAg                               >  1:3832978/1‑51 (MQ=255)                 ttGGCTAAATTGTTGAATAACAGACAGTGCCAATTCATCATTCTCAATCAg                               <  1:2471151/51‑1 (MQ=255)                 ttGGCTAAATTGTTGAATAACAGACAGTGCCAATTCATCATTCTCAATCAg                               >  1:1101751/1‑51 (MQ=255)                 ttGGCTAAATTGTTGAATAACAGACAGTGCCAATTCATCATTCTCAATCAg                               >  1:2278509/1‑51 (MQ=255)                  tGGCTAAATTGTTGAATAACAGACAGTGCCAATTCATCATTCTCAATCAGa                              <  1:1893498/51‑1 (MQ=255)                  tGGCTAAATTGTTGAATAACAGACAGTGCCAATTCATCATTCTCAATCAGa                              >  1:3399596/1‑51 (MQ=255)                   ggCTAAATTGTTGAATAACAGACAGTGCCAATTCATCATTCTCAATCAGAc                             >  1:1726300/1‑51 (MQ=255)                   ggCTAAATTGTTGAATAACAGACAGTGCCAATTCATCATTCTCAATCAGAc                             >  1:5834375/1‑51 (MQ=255)                    gCTAAATTGTTGAATAACAGACAGTGCCAATTCATCATTCTCAATCAGACa                            >  1:5773101/1‑51 (MQ=255)                     cTAAATTGTTGAATAACAGACAGTGCCAATTCATCATTCTCAATCAGACaa                           <  1:7209160/51‑1 (MQ=255)                      tAAATTGTTGAATAACAGACAGTGCCAATTCATCATTCTCAATCAGACAAc                          >  1:365097/1‑51 (MQ=255)                      tAAATTGTTGAATAACAGACAGTGCCAATTCATCATTCTCAATCAGACAAc                          >  1:1970292/1‑51 (MQ=255)                      tAAATTGTTGAATAACAGACAGTGCCAATTCATCATTCTCAATCAGACAAc                          >  1:5858558/1‑51 (MQ=255)                       aaaTTGTTGAATAACAGACAGTGCCAATTCATCATTCTCAATCAGACAAct                         >  1:4293192/1‑51 (MQ=255)                        aaTTGTTGAATAACAGACAGTGCCAATTCATCATTCTCAATCAGACAActc                        >  1:7101348/1‑51 (MQ=255)                        aaTTGTTGAATAACAGACAGTGCCAATTCATCATTCTCAATCAGACAActc                        >  1:3674820/1‑51 (MQ=255)                          ttgttgAATAACAGACAGTGCCAATTCATCATTCTCAATCAGACAActctc                      >  1:1741145/1‑51 (MQ=255)                           tgttgAATAACAGACAGTGCCAATTCATCATTCTCAATCAGACAActctct                     >  1:646235/1‑51 (MQ=255)                           tgttgAATAACAGACAGTGCCAATTCATCATTCTCAATCAGACAActctct                     <  1:5451431/51‑1 (MQ=255)                           tgttgAATAACAGACAGTGCCAATTCATCATTCTCAATCAGACAActctct                     >  1:6996506/1‑51 (MQ=255)                            gttgAATAACAGACAGTGCCAATTCATCATTCTCAATCAGACAACTCTCTg                    >  1:2356615/1‑51 (MQ=255)                             ttgAATAACAGACAGTGCCAATTCATCATTCTCAATCAGACAACTCTCTGt                   >  1:2516666/1‑51 (MQ=255)                             ttgAATAACAGACAGTGCCAATTCATCATTCTCAATCAGACAACTCTCTGt                   >  1:6669949/1‑51 (MQ=255)                               gAATAACAGACAGTGCCAATTCATCATTCTCAATCAGACAACTCTCTGTCa                 <  1:1613410/51‑1 (MQ=255)                                aaTAACAGACAGTGCCAATTCATCATTCTCAATCAGACAACTCTCTGTCAg                <  1:778059/51‑1 (MQ=255)                                 aTAACAGACAGTGCCAATTCATCATTCTCAATCAGACAACTCTCTGTCAGt               >  1:5694510/1‑51 (MQ=255)                                  tAACAGACAGTGCCAATTCATCATTCTCAATCAGACAACTCTCTGTCAGtt              <  1:4479308/51‑1 (MQ=255)                                  tAACAGACAGTGCCAATTCATCATTCTCAATCAGACAACTCTCTGTCAGtt              >  1:3535939/1‑51 (MQ=255)                                   aaCAGACAGTGCCAATTCATCATTCTCAATCAGACAACTCTCTGTCAGTTc             >  1:2475406/1‑51 (MQ=255)                                    acagacagTGCCAATTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCa            <  1:1097176/51‑1 (MQ=255)                                     cagacagTGCCAATTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCaa           >  1:6715717/1‑51 (MQ=255)                                     cagacagTGCCAATTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCaa           >  1:5235104/1‑51 (MQ=255)                                     cagacagTGCCAATTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCaa           <  1:3369553/51‑1 (MQ=255)                                      atacagTGCCAATTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAAc          >  1:6193557/3‑51 (MQ=255)                                      agacagTGCCAATTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAAc          <  1:6053854/51‑1 (MQ=255)                                       gacagTGCCAATTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACa         <  1:4908665/51‑1 (MQ=255)                                       gacagTGCCAATTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACa         >  1:1152376/1‑51 (MQ=255)                                        acagTGCCAATTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACAt        <  1:202247/51‑1 (MQ=255)                                         cagTGCCAATTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATc       >  1:3949516/1‑51 (MQ=255)                                         cagTGCCAATTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATc       <  1:274795/51‑1 (MQ=255)                                          agTGCCAATTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCt      >  1:3111906/1‑51 (MQ=255)                                          agTGCCAATTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCt      >  1:2414082/1‑51 (MQ=255)                                           gTGCCAATTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCta     >  1:6350244/1‑51 (MQ=255)                                             gCCAATTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCtata   <  1:4183731/51‑1 (MQ=255)                                             gCCAATTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCtata   >  1:5367363/1‑51 (MQ=255)                                              ccAATTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCtata   <  1:1467714/50‑1 (MQ=255)                                              ccAATTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATNg  <  1:2551834/51‑1 (MQ=255)                                              ccAATTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAg  >  1:3079271/1‑51 (MQ=255)                                                 |                                                GTACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAG  >  REL606/1343441‑1343536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑