Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	761,693	T→G	noncoding (47/76 nt)	valT →	tRNA‑Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	761,693	0	T	G	100.0%	80.4 / NA	27	noncoding (47/76 nt)	valT	tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (16/11);  total (16/11)

TTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCA  >  REL606/761645‑761738                                                 |                                              ttGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGcg                                             <  1:5705225/51‑1 (MQ=9)                              cTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCaccacc                >  1:4556850/1‑51 (MQ=9)                              cTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCaccacc                <  1:5097971/51‑1 (MQ=9)                                cccTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCgg              <  1:2532815/51‑1 (MQ=255)                                 ccTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCggg             >  1:5788087/1‑51 (MQ=255)                                 ccTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCggg             >  1:4518548/1‑51 (MQ=255)                                 ccTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCggg             >  1:2176831/1‑51 (MQ=255)                                 ccTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCggg             <  1:2417319/51‑1 (MQ=255)                                 ccTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCggg             <  1:2959045/51‑1 (MQ=255)                                  cTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGt            <  1:1563778/51‑1 (MQ=255)                                   ttACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCGCCCACCACCGGGTc           <  1:6537703/51‑1 (MQ=14)                                   ttACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCggg             >  1:1609026/1‑49 (MQ=255)                                   ttACAAGGAGGGGGGCGGCGGTTCGATCCCATCATCACCCACCACCGGGTc           >  1:2638330/1‑51 (MQ=14)                                    tACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTc           >  1:5741410/1‑50 (MQ=255)                                    tACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCg          >  1:3261969/1‑51 (MQ=255)                                    tACAAGGAGGGGGGCAGCGGTTCGATCCCGTCATCACCCaccacc                >  1:1282872/1‑45 (MQ=11)                                      cAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGtt        <  1:3383515/51‑1 (MQ=255)                                      cAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGtt        >  1:2521797/1‑51 (MQ=255)                                       aaGGAGGGGGGCGGCGGTTTGATCCCGTCATCACCCACCACCGGGTCGTTa       >  1:6988787/1‑51 (MQ=14)                                       aaGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCaccacc                <  1:2127278/42‑1 (MQ=12)                                       aaGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTa       >  1:889181/1‑51 (MQ=255)                                       aaGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTa       >  1:864304/1‑51 (MQ=255)                                       aaGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTa       >  1:1368282/1‑51 (MQ=255)                                       aaGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTa       <  1:5262567/51‑1 (MQ=255)                                       aaGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTa       >  1:6059958/1‑51 (MQ=255)                                         ggaggGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGc     >  1:2867317/1‑51 (MQ=255)                                           aggGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTc   <  1:606842/51‑1 (MQ=255)                                            ggggggCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTc   <  1:5192031/50‑1 (MQ=255)                                            ggggggCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCa  >  1:2167040/1‑51 (MQ=255)                                            ggggggCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCa  <  1:6921055/51‑1 (MQ=255)                                            ggggggCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCa  <  1:73980/51‑1 (MQ=255)                                            ggggggCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCa  <  1:5362996/51‑1 (MQ=255)                                            ggggggCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCa  <  1:5304973/51‑1 (MQ=255)                                                 |                                              TTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCA  >  REL606/761645‑761738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑