Predicted mutation
evidence seq id position mutation freq annotation gene description
RA REL606 4,384,430 C→A 100% S29Y (TCT→TAT)  yjeB → predicted DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6064,384,4300CA100.0% 118.3 / NA 36S29Y (TCT→TAT) yjeBpredicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/13);  total (23/13)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTCTGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATGGCGGCATTCGCCTGGGTAAATCGGCGAGTGCGATACGTATTGGTG  >  REL606/4384283‑4384576
                                                                                                                                                   |                                                                                                                                                  
cTTGTTGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGTTTTGCAGGTAACGACTTTCGCGGATTACGGACTACGGGCGATGATCTATTAGGTGTCAGTGCCAGATGGGGGGATGCCCAGTGTTTATg                                                                                                                                                  <  1:486690/150‑1 (MQ=255)
cTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATg                                                                                                                                                  <  2:299719/150‑1 (MQ=255)
cTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATg                                                                                                                                                  <  1:277141/150‑1 (MQ=255)
   gtgGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAg                                                                                                                                               <  2:518049/150‑1 (MQ=255)
                     atatAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGCGTTTCACTAATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTTCGAAGTGACCGTCGTCTACGGCg                                                                                                                             >  1:166929/1‑150 (MQ=255)
                            tGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCg                                                                                                                      <  2:80543/150‑1 (MQ=255)
                                 tttttCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATc                                                                                                                 <  2:636677/150‑1 (MQ=255)
                                    ttCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCata                                                                                                              <  2:437127/150‑1 (MQ=255)
                                     tCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCatat                                                                                                             <  2:214824/150‑1 (MQ=255)
                                                           gATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGCACAGTATTTATGAAGTAACTGACGTCTACGGCGTCTCCCGTAATCATATGGGCAAAATCATCAATGAAGtt                                                                                       >  1:676103/1‑150 (MQ=255)
                                                           gATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACtt                                                                                       >  1:512713/1‑150 (MQ=255)
                                                                gCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCg                                                                                  <  2:143853/150‑1 (MQ=255)
                                                                   gTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTga                                                                               >  1:600722/1‑149 (MQ=255)
                                                                             ttCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTg                                                                     <  1:526950/150‑1 (MQ=255)
                                                                                          gattacgTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTg                                                        >  2:74713/1‑150 (MQ=255)
                                                                                                     cTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTCTTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGACAAAATAATCAATCAACTTAGACGTGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATggc                                             >  1:746/1‑150 (MQ=255)
                                                                                                     cTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATggc                                             >  2:418177/1‑150 (MQ=255)
                                                                                                     cTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATggc                                             >  2:447875/1‑150 (MQ=255)
                                                                                                       gATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTGCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATggcgg                                           >  1:543091/1‑150 (MQ=255)
                                                                                                       gATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCATTCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGGGGAAAAAATgtcgg                                           >  1:260427/1‑150 (MQ=255)
                                                                                                       gATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATggcgg                                           >  2:618275/1‑150 (MQ=255)
                                                                                                       gATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATggcgg                                           >  1:164442/1‑150 (MQ=255)
                                                                                                       gATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATggcgg                                           >  2:596954/1‑150 (MQ=255)
                                                                                                       gATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATggcgg                                           >  2:533542/1‑150 (MQ=255)
                                                                                                       gATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGCCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCCGCTACGTCACTGCTGTTCGTGGAAAAAATggcgg                                           >  1:447991/1‑150 (MQ=255)
                                                                                                            aCATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATGGCGGCATTc                                      >  2:106033/1‑150 (MQ=255)
                                                                                                                         gCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATGGCGGCATTCGCCTGGGTAAATc                         <  2:422466/150‑1 (MQ=255)
                                                                                                                           cAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATGGCGGCATTCGCCTGGGTAAATCgg                       <  2:75711/150‑1 (MQ=255)
                                                                                                                                        gACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATGGCGGCATTCGCCTGGGTAAATCGGCGAGTGCGATACg          >  1:407151/1‑150 (MQ=255)
                                                                                                                                          caggTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATGGCGGCATTCGCCTGGGTAAATCGGCGAGTGCGATACGTa        <  1:324735/147‑1 (MQ=255)
                                                                                                                                             gTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATGGCGGCATTCGCCTGGGTAAATCGGCGAGTGCGATACGTactg     >  2:325657/1‑147 (MQ=255)
                                                                                                                                             gTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATGGCGGCATTCGCCTGGGTAAATCGGCGAGTGCGATACGTATTg     >  1:552712/1‑150 (MQ=255)
                                                                                                                                             gTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGGGGAAAAAATGGCGGCATTCGCCTGGGTAAATCGGCGAGTGCGATACGTATTg     >  1:285058/1‑150 (MQ=255)
                                                                                                                                                tttATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATGGCGGCATTCGCCTGGGTAAATCGGCGAGTGCGATACGTATTGGTg  >  2:161665/1‑150 (MQ=255)
                                                                                                                                                tttATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATGGCGGCATTCGCCTGGGTAAATCGGCGAGTGCGATACGTATTGGTg  >  1:163359/1‑150 (MQ=255)
                                                                                                                                                tttATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATGGCGGCATTCGCCTGGGTAAATCGGCGAGTGCGATACGTATTGGTg  >  1:402402/1‑150 (MQ=255)
                                                                                                                                                tttATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATGGCGGCATTCGCCTGGGTAAATCGGCGAGTGCGATACGTATTGGTg  >  1:123507/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTCTGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAAAAATGGCGGCATTCGCCTGGGTAAATCGGCGAGTGCGATACGTATTGGTG  >  REL606/4384283‑4384576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑