| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 4,201,980 | G→A | 100% | P194S (CCT→TCT) | iclR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,201,980 | 0 | G | A | 100.0% | 111.5 / NA | 34 | P194S (CCT→TCT) | iclR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/20); total (14/20) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTGCAGCAGCTTCGTCACCTGTTCTTCGCTCAGTTGGGCTAAAAAGGCTTTACCTGCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACA > REL606/4201833‑4202123 | gTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGaag < 2:92641/150‑1 (MQ=255) gAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCTAGATCTTCTTTTAAATGCACAGAAGACAcc < 2:431566/150‑1 (MQ=255) gAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACAcc < 1:266106/150‑1 (MQ=255) gAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACAcc < 2:33895/150‑1 (MQ=255) gAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACAcc < 2:2426/150‑1 (MQ=255) gAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACAcc < 1:11813/150‑1 (MQ=255) gAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTGCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACAcc < 1:625166/150‑1 (MQ=255) ttGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGTCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGt < 1:426975/150‑1 (MQ=255) gCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTgg < 2:200529/150‑1 (MQ=255) cGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTggg < 2:491932/150‑1 (MQ=255) gCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGta < 2:81866/150‑1 (MQ=255) cGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGATGACACCAGCGTTGCGTGGGTCTAGGc > 1:530267/1‑150 (MQ=255) cGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGc < 2:426205/150‑1 (MQ=255) aggTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGc < 2:408630/149‑1 (MQ=255) ggTTCACGGTGTTCATCGAAAATACACGCTGCAAGGTAACGTAGCCCCGGCGCATGTTCCTCATCGTCAAATGAATAACCACGTGTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACAGCAGCGTTGCGTGGGTATAGGCa < 1:174968/150‑1 (MQ=255) gTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGcct > 1:407457/1‑148 (MQ=255) cACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTa > 1:88962/1‑150 (MQ=255) ggTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAAcc < 2:367917/150‑1 (MQ=255) gTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAAccc < 1:20871/150‑1 (MQ=255) gTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAAccc < 2:286024/150‑1 (MQ=255) cATCGAACATACACGCTTCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGc < 1:101693/150‑1 (MQ=255) acacGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTGcagcag < 2:89222/150‑1 (MQ=255) cTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTGCAGCAGCTTCg > 2:78923/1‑150 (MQ=255) cTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTGTAAATGCACAGAAGACACCAGCGTTGCGTGGGTCTAGGCATGTAACCCGTTGCGGTGATGCAGCTTCg > 1:372868/1‑150 (MQ=255) aGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTGCAGCAGCTTCGTCAcc > 2:65854/1‑150 (MQ=255) aGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAAACCCGTTTGCGCGTTTGGGCGAGATCTTCGTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTATCCCCTTGCGGTGAAGCAGCTTCGTCAcc > 1:253906/1‑150 (MQ=255) aCGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGCTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTTCGGTGCAGCAGCTTCGTCACCTGttc > 1:283000/1‑150 (MQ=255) aTGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTGCAGCAGCTTCGTCACCTGTTCTTCGCTCAGTTGGGc < 1:15072/150‑1 (MQ=255) gAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTGCAGCAGCTTCGTCACCTGTTCTTCGCTCAGTTGGGCTAAAAAGGCTTTACCTGCa > 1:180221/1‑150 (MQ=255) gAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTGCAGCAGCTTCGTCACCTGTTCTTCGCTCAGTTGGGCTAAAAAGGCTTTACCTGCa > 1:48088/1‑150 (MQ=255) ggCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTGCAGCAGCTTCGTCACCTGTTCTTCGCTCAGTTGGGCTAAAAAGGCTTTACCTGCACCGGAAGCGTGCATCGGCAAttt < 2:48234/150‑1 (MQ=255) ggCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTGCAGCAGCTTCGTCACCTGTTCTTCGCTCAGTTGGGCTAAAAAGGCTTTACCTGCACCGGAAGCGTGCATCGGCAAttt > 2:27356/1‑150 (MQ=255) ggCGAGATCTTCTTTTAAATGCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTGCAGCAGCTTCGTCACCTGTTCTTCGCTCAGTTGGGCTAAAAAGGCTTTACCTGCACCGGAAGCGTGCATCGGCAAtat > 1:218884/1‑148 (MQ=255) gCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGTTGCAGCAGCTTCGTCCCCTGTTCTTCGCCCAGTTGGGCGAGAAAGGCTTTACCTGCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACa > 1:92825/1‑150 (MQ=255) gCACAGAAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTGCCGCAGCTTCGTCACCTGTTCTTCGCTCAGTTGGGCAAAAAAGGCATTACCTGCACCGGAAGCGTGCATCGGCAATTTAACGACGATAGGCGCGGACa > 1:640691/1‑150 (MQ=255) | GTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTGCAGCAGCTTCGTCACCTGTTCTTCGCTCAGTTGGGCTAAAAAGGCTTTACCTGCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACA > REL606/4201833‑4202123 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |