| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 4,096,877 | G→T | 100% | Y232* (TAC→TAA) | glpF ← | glycerol facilitator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,096,877 | 0 | G | T | 96.8% | 98.9 / ‑2.8 | 32 | Y232* (TAC→TAA) | glpF | glycerol facilitator |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (14/16); total (16/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACC > REL606/4096733‑4097023 | cGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAgg < 1:16411/150‑1 (MQ=255)cGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAgg < 1:59585/150‑1 (MQ=255) gCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATg > 1:678960/1‑150 (MQ=255) ttttGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGtct < 1:194065/150‑1 (MQ=255) gTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTgc > 1:457215/1‑150 (MQ=255) ttttCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGccc < 2:651068/150‑1 (MQ=255) tttCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGcccc < 2:680956/150‑1 (MQ=255) ttcCACAACACAGATATCGCAAGGCAAATGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGcc < 2:39462/150‑1 (MQ=255) ggCAAATGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTACGGAATGACTCTGCCGCCGGTAAAGGCGACATTGCCCCAACCCGCCAGCCAGGCAAACACTTTc > 1:406304/1‑150 (MQ=255) ggCAAATGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTc > 2:601336/1‑150 (MQ=255) ggCAAATGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCC‑AAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATGGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCg > 1:424335/1‑150 (MQ=255) ccAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTTCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCa < 1:336250/150‑1 (MQ=255) gTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTg > 2:650884/1‑150 (MQ=255) gTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTg > 2:147751/1‑150 (MQ=255) agacagtGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGc < 2:252195/144‑1 (MQ=255) tGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCAGGGcctgtct > 1:251475/1‑144 (MQ=255) ccTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTctgtctcttatacaca > 1:226456/1‑135 (MQ=255) ccTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTc > 2:94679/1‑150 (MQ=255) acgattgagcCAACTATAGGGCCAACAAGCGGCACCAGGAAGTAAGTAATGTCTCTGCCGCCGGTAAATGTAAAAGTGCCCCAGCCCGCCAGCCCGGCAAAGACTTTCGGAGCGAAATCACGCGCTGGGTTCATCTCAAAACCTGTAAAt < 1:425437/142‑1 (MQ=255) cAACGATAGGGCC‑AAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGa < 2:425543/150‑1 (MQ=255) aaCGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCACAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGa < 2:429017/150‑1 (MQ=255) aaCGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGa < 2:119133/150‑1 (MQ=255) aaCGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGa > 2:588285/1‑150 (MQ=255) aaCGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGa < 2:239824/150‑1 (MQ=255) aaCGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGa < 2:230662/150‑1 (MQ=255) aaCGATAGGGCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGa < 2:458517/150‑1 (MQ=255) aaCGAAAGGGCATAAAAGCGGCACCAGGAATTAAGGAATGTTTCTGCCGCCGGTAAAGGCGACATTGCCCCAGACCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGa < 1:215981/150‑1 (MQ=255) cGATAGGGCCAAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGATg > 2:28288/1‑150 (MQ=255) gCCAAAAAGCGGCACCAGGAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGATGCGCCAAt > 1:498311/1‑150 (MQ=255) ggAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGGAAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGAc < 1:147327/150‑1 (MQ=255) ggAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGAc > 1:250303/1‑150 (MQ=255) gAATTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGAcc > 1:377588/1‑150 (MQ=255) gAAATAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCACCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGACGTCACGCGCTGGGCAAATGGCAAAACCCGTCAAAGGCCCCACCATGGCGCCAATGAGCGCCAGCAGTaaccc > 1:311006/1‑146 (MQ=255) | CGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACC > REL606/4096733‑4097023 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |