Predicted mutation
evidence seq id position mutation freq annotation gene description
RA REL606 3,396,621 T→G 63.1% N380H (AAC→CAC)  chiA ← periplasmic endochitinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6063,396,6210TG63.1% 24.8 / 24.4 27N380H (AAC→CAC) chiAperiplasmic endochitinase
Reads supporting (aligned to +/- strand):  ref base T (3/7);  new base G (10/7);  total (13/14)
Fisher's exact test for biased strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

ATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCG  >  REL606/3396472‑3396760
                                                                                                                                                      |                                                                                                                                           
ataATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGtt                                                                                                                                             <  2:17358/150‑1 (MQ=255)
    tCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTg                                                                                                                                         >  2:479356/1‑150 (MQ=255)
                     gcTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGt                                                                                                                        >  1:531130/1‑150 (MQ=255)
                        gTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTGATTGGAGTTTTCAGCGCTGGTAc                                                                                                                      >  2:224691/1‑150 (MQ=255)
                        gTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTGATTGGAGTTTTCAGCGCTGGTAc                                                                                                                      <  1:224050/150‑1 (MQ=255)
                         tACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTGATTGGAGTTTTCAGCGCTGGTAccc                                                                                                                    <  2:4094/150‑1 (MQ=255)
                         tACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTGATTGGAGTTTTCAGCGCTGGTAccc                                                                                                                    <  2:212786/150‑1 (MQ=255)
                           cGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCg                                                                                                                  <  2:319869/150‑1 (MQ=255)
                               ttGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTGATTGGAGTTTTCAGCGCTGGTACCCCGTggg                                                                                                              >  2:130650/1‑150 (MQ=255)
                                tGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCTATGGGTGATTGGAGTTTTCAGCGCTGGTACCCCGTGGGc                                                                                                             <  2:85630/150‑1 (MQ=255)
                                      ccTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTGATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCtt                                                                                                       >  2:543709/1‑150 (MQ=255)
                                            aaCCATCCGTAACACTGAGTCCGCCGTCATCAATTTCACAGGTCGTCGGGTTGCAGTACTGACTCAATTCCGCTGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTTGAGTTTTCAGCGCTGGTACCCCGTTGTCAATCTTTATacc                                                                                                 <  1:536086/150‑1 (MQ=255)
                                            aaCCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGacc                                                                                                 <  2:532668/150‑1 (MQ=255)
                                            aaCCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGacc                                                                                                 <  2:136519/150‑1 (MQ=255)
                                                          aTGACGCCGCCGTTAGCACTTTCACAGGTCGTCGTTCTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTCCGCTCGAGGCGCTATGCCTGATTTGCGTTTTCAGCGCTCGTACCACCTCGGCAAGCTTTAGACCCACCCCACCAGGCg                                                                                   <  1:359766/150‑1 (MQ=255)
                                                                   ccgTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTaa                                                                          <  1:478001/150‑1 (MQ=255)
                                                                     gatATCAACTGCACAGCTTGGCGGGTTACCCTACCGACTCACTTCCGCGGCTGTACCCGTCCCCTCGAGACCCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAAc                                                                        <  1:19088/148‑1 (MQ=255)
                                                                     gTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAAc                                                                        <  2:146126/150‑1 (MQ=255)
                                                                     gTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTGATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAAc                                                                        <  2:339404/150‑1 (MQ=255)
                                                                     gTTAGCAATTTCACAGGTGCTCGGGTTACCGTACTGACTCAATGCCGCAGCGGTAGCTGTACAGTCGAGACGCCATGGGTGATTGGAGTTTTCAGCGCTGGTTCCCCGTGGGCAATCTTTAGACCCTTCCCGCCAGGCGTTCTTGTAAAc                                                                        <  1:458293/150‑1 (MQ=255)
                                                                             tttCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTGATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCAt                                                                >  2:195709/1‑150 (MQ=255)
                                                                                                 aCCGTACTGACTCCATTCCGCGGCGGTAGCTCTACGCTCGAGACGCCCTGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCTCCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAAt                                             <  1:529803/150‑1 (MQ=255)
                                                                                                    gTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTc                                          >  1:577049/1‑150 (MQ=255)
                                                                                                               aTTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTGATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCt                               >  2:25891/1‑150 (MQ=255)
                                                                                                                       gCGGTAGCTGTACGCTCGAGACGCCATGGGTGATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTc                       >  1:624129/1‑150 (MQ=255)
                                                                                                                             gCTGTACGCTCGAGACGCCATGGGTGATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCt                 >  2:108118/1‑150 (MQ=255)
                                                                                                                             gCTGTACGCTCGAGACGCCATGGGTGATTGGAGCTTTCAGCGCTGCTACCCCGTGGGCACTCTTTAGACCCAACCCACCAGGCGTTCGTGTAAACATTACCATTGAAAATAACATGGCAAATTTCACTACCTTCCTGGCCGCTCCCCGCt                 >  1:120551/1‑150 (MQ=255)
                                                                                                                                gTACGCTCGAGACGCCATGGGTGATTGGAGTTTTCAGCGCTGCTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAACCATTACCATTGAAAATAACATGGTAACTTTGGCTACCTTCCTGGCCGCTCCACGATTGc              >  1:476723/1‑150 (MQ=255)
                                                                                                                                            cGCCATGGGTGATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCg  <  2:478076/150‑1 (MQ=255)
                                                                                                                                            cGCCATGGGTGATTGGAGTTTTCAGCGCTGGTACCCCCTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCg  >  2:31222/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                           
ATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCG  >  REL606/3396472‑3396760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: