| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 3,328,272 | A→G | 100% | V236A (GTT→GCT) | mreB ← | cell wall structural complex MreBCD, actin‑like component MreB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,328,272 | 0 | A | G | 98.3% | 96.7 / ‑6.4 | 30 | V236A (GTT→GCT) | mreB | cell wall structural complex MreBCD, actin‑like component MreB |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (17/12); total (17/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATG > REL606/3328135‑3328415 | gTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCAc > 2:473914/1‑150 (MQ=255) ccGGCGGGCACTGTTCCAGTGCGACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGGGCTTCGAGGATTTCATTTGAGTTAAGGGTAAAACCGCGTGGAAGACCTTCTGCCATGTTGCGGCCACGAGCTTCGACTTCACGGa > 1:457321/1‑150 (MQ=255) ggcggGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACt > 1:109229/1‑150 (MQ=255) gCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATc > 2:444391/1‑150 (MQ=255) cACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCg > 2:254823/1‑150 (MQ=255) cACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCg > 2:662129/1‑150 (MQ=255) cTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGcc > 1:230539/1‑150 (MQ=255) gTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCg > 2:297118/1‑150 (MQ=255) gTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACCAGCTTCGATTTCACGGACTTCATCGCCCg > 1:96102/1‑150 (MQ=255) gTTCCAGTGCAACAATTACAGCGCACACAATACCGGTCAGCGGTTCCTGCAGTACTTCGAGGATTTCATTGGAGTTCAGGATAACACCGCGTGGAACACCTTCTGCCAGGTTACGGCAACGAGCTTCGATTTCCCGGACTTAATCGCCCg > 1:606225/1‑150 (MQ=255) ccATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAAc > 1:108028/1‑150 (MQ=255) cacaATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCtt > 2:651622/1‑150 (MQ=255) aTACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGGGCTTGATa > 1:70772/1‑150 (MQ=255) ggTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTc < 2:93605/150‑1 (MQ=255) gCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCgg < 2:234196/150‑1 (MQ=255) cAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCAcc < 2:109551/150‑1 (MQ=255) cAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCAcc < 2:315114/150‑1 (MQ=255) cgcttgcATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTGACGGCCACGAACTTCGACTGCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAAc < 1:559239/144‑1 (MQ=255) tttCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGt > 2:343144/1‑150 (MQ=255) gggTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACAt > 1:491162/1‑150 (MQ=255) gggTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGACCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACAt > 1:600510/1‑150 (MQ=255) gggTAAAACCGCGGGGAACACCTTCTGCCAGGTTACGGCCACGGGCTTCGATTTCACGCACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACAt > 2:400525/1‑150 (MQ=255) aaaaCCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGtt < 2:150163/150‑1 (MQ=255) aaCCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTTATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTtga < 1:404267/150‑1 (MQ=255) aCCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTtgat > 1:444317/1‑150 (MQ=255) gcgTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTgatgat < 1:472570/150‑1 (MQ=255) gcgTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTgatgat < 2:96389/150‑1 (MQ=255) gcgTGGAACACCTTCTGCCAGGTTACGGCCACGAGCTGCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAgatgatgat < 1:239818/150‑1 (MQ=255) gcgTGGAACACCTTCTGCCAGGTTACGACCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTgatgat < 2:602277/150‑1 (MQ=255) ggTTACGGCCACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTcac > 2:523661/1‑150 (MQ=255) aCGGCCACGAGCGTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCaccacc < 1:522160/150‑1 (MQ=255) ggCCCCGAGCTTCTCTTTCACGGACTTCATCGCCCAGATAAGCCGAAGCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGTTTCGTCGAAACGGTCACCCCCaa < 1:400322/150‑1 (MQ=255) ccACGGGCTTCGATTTCACGGACTTCAGCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGGTTCGCCGGTCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCaccccctacg < 1:40044/150‑5 (MQ=255) ccACGAGCTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATg < 2:492557/150‑1 (MQ=255) | GTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTCACAATACCGGTCAGCGGTTCCTGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATG > REL606/3328135‑3328415 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |