| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 3,312,775 | G→A | 100% | R59H (CGC→CAC) | argR → | arginine repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,312,775 | 0 | G | A | 100.0% | 61.1 / NA | 19 | R59H (CGC→CAC) | argR | arginine repressor |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (11/8); total (11/8) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGTTAAAGCATTTAAAGCATTACTTAAAGAAGAGAAATTTAGCTCCCAGGGCGAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACGCAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAAAATCTGGTGCTGGATATCGACTACAACGATGCAGTTGTCGTGATTCATACCAGCCCTGGCGCGG > REL606/3312629‑3312912 | aGTTAAAGCATTTAAAGCATTACTTAAAGAAGAGAAATTTAGCTCCCAGGGCGAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACaa > 1:560202/1‑150 (MQ=255) aCTTAAAGAAGAGAAATTTAGCTCCCAGGGCGAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTTGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTa > 1:81429/1‑150 (MQ=255) cTTAGAGAAGAGAAATTTATCTCCCAGTGCGAAATCGTCGCCGCGTTGCAGTAGCAAGGCTTTGACAATATGAATCATTCTAACGTCTCGCGGATGGTGACCAAGGTTGGATCTGTACGTACACCCCGTGCCCAACTGGCAAGGGCTTAc > 1:7037/1‑150 (MQ=255) tAAAGAAGAGAAATTTAGCTCCCAGGGCGAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTActg < 2:76299/150‑1 (MQ=255) aaTTTAGGTTACAGGGCGAAATCGTCGCCACGTAGCAGGAGCAAGGCTTTGACAATCTTAATCAGTCTAAAGTCTCGCCGATGTTGACCAAGGTTGGTGCTGTACGTACACACAATGCGAAAATGAAAATGGTTTACTGCCTGCTAGcgg < 1:317621/150‑3 (MQ=255) gggCGAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTacc < 2:561824/150‑1 (MQ=255) gACAAGATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGTTGCTGTACGTACACACAATTCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCATTCCATTGAAAACTCTGGTGCTGGat < 1:582729/150‑1 (MQ=255) atTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTCGCTGAAATGGGTGTACCAACCACCTCCAGTCCATTGAAAAATCTGGTTCTGGATACCGAc > 1:662688/1‑150 (MQ=255) atTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAAAATCTGGTGCTGGATATCGAc < 2:51830/150‑1 (MQ=255) gTCTAAAGTCTCTCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAATTGGAAATGGTTTACTGCCTGCTAGCTTAACTGGGTGTACCAACCTCCGCCAGTCCATTAAAAACTCGGGTGCTGGATATAGATTACacagt > 1:23158/1‑146 (MQ=255) gTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAAAATCTGGTGCTGGATATCGACTACAACGa > 1:465143/1‑150 (MQ=255) gTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAAAATCTGGTGCTGGATATCGACTACAACGa > 2:307701/1‑150 (MQ=255) gTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAAAATCTGGTGCTGGATATCGACTACAACGa > 2:337739/1‑150 (MQ=255) gTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAAAATCTGGTGCTGGATATCGACTACAACGa > 2:403004/1‑150 (MQ=255) gATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGCAATGGTTTACTGCATGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAAAATCTGGTGCTGGATATCGACTACAACGATGCAGTTGTCGTGAt > 1:428072/1‑150 (MQ=255) aCCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAAAATCTGGTGCTGGATATCGACTACAACGATGCAGTTGTCGTGATTCATAcc < 1:401843/150‑1 (MQ=255) gTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAAAATCTGGTGCTGGATATCGACTACAACGATGCAGTTGTCGTGATTCATACCAGccc > 2:544981/1‑150 (MQ=255) gTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAAAATCTGGTGCTGGATATCGACTACAACGATGCAGTTGTCGTGATTCATACCAGccc < 2:466466/150‑1 (MQ=255) gTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAAAATCTGGTGCTGGATATCGACTACAACGATGCAGTTGTCGTGATTCATACCAGCCCTggcg < 1:543403/150‑1 (MQ=255) cTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAAAATCTGGTGCTGGATATCGAATAAAACGATGCAGTTCTCGTGAATCATACACTCCCTggcgcgg > 1:510413/1‑150 (MQ=255) | AGTTAAAGCATTTAAAGCATTACTTAAAGAAGAGAAATTTAGCTCCCAGGGCGAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACGCAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAAAATCTGGTGCTGGATATCGACTACAACGATGCAGTTGTCGTGATTCATACCAGCCCTGGCGCGG > REL606/3312629‑3312912 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |