| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 3,287,483 | T→C | 100% | M260V (ATG→GTG) | arcB ← | hybrid sensory histidine kinase in two‑component regulatory system with ArcA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,287,483 | 0 | T | C | 99.5% | 113.1 / ‑7.0 | 31 | M260V (ATG→GTG) | arcB | hybrid sensory histidine kinase in two‑component regulatory system with ArcA |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (13/17); total (13/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCTCAGGCCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAAATAAACGTCGTTTTGTCGCGGCTGGCGCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACAAATTTCAAAGCAGGCTTTGCGTCCGTCGGGATAATCCAGCCACTGCTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGAC > REL606/3287346‑3287627 | gCTCAGGCCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAAATAAACGTCGTTTTGTCGCGGCTGGCGCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCTCACCAAACCGTGACg > 2:230393/1‑150 (MQ=255) ggCCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAAATAAACGTCGTTTTGTCGCGGCTGGCGCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTAc > 2:388728/1‑150 (MQ=255) acgaTACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAAATAAACGTCGTTTTGTCGCGGCTGGCGCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCAcg < 1:588546/150‑1 (MQ=255) ccGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAAATAAACGTCGTTTTGTCGCGGCTGGCGCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCg > 2:580745/1‑150 (MQ=255) gTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAAATAAACGTCGTTTTGTCGCGGCTGGCGCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCgta > 1:256327/1‑150 (MQ=255) gTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAAATAAACGTCGTTTTGTCGCGGCTGGCGCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCgta > 2:312390/1‑150 (MQ=255) cAGCGGTGTACGCAATTCGTGACTGATGGTGGAAATAAACGTCGTTTTGTCGCGGCTGGCGCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCgtagta < 2:182754/150‑1 (MQ=255) aGCGGTGTACGCAATTCGTGACTGATGGTGGAAATAAACGTCGTTTTGTCGCGGCTGGCGCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTAc < 1:579045/150‑1 (MQ=255) aGCGGTGTACGCAATTCGTGACTGATGGTGGAAATAAACGTCGTTTTGTCGCGGCTGGCGCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTAc < 2:401317/150‑1 (MQ=255) ggTGTACGCAATTCGTGACTGATGGTGGAAATAAACGTCGTTTTGTCGCGGCTGGCGCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGc > 2:637019/1‑150 (MQ=255) ggTGTACGCAATTCGTGACTGATGGTGGAAATAAACGTCGTTTTGTCGCGGCTGGCGCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGc < 1:635154/150‑1 (MQ=255) cAATTCGTGACTGATGGTGGAAATAAACGTCGTTTTGTCGCGGCTGGCGCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACa > 2:672640/1‑150 (MQ=255) aTTCGTGACTGATGGTGGAAATAAACGTCGTTTTGTCGCGGCTGGCGCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACaaa < 2:158024/150‑1 (MQ=255) aaTAAACGTCGTTTTGTCGCGGCTGGCGCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACAAATTTCAAAGCAGGCTTTGcg < 1:526159/150‑1 (MQ=255) ttttGTCGCGGCTGGCGCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCTCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACAAATTTCAAAGCAGGCTTTGCGTCCGTCGGGAt < 1:229741/150‑1 (MQ=255) tCGCGGCTGGCGCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACAAATTTCAAAGCAGGCTTTGCGTCCGTCGGGATAATcc < 2:265419/150‑1 (MQ=255) ggCTGGCGCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACAAATTTCAAAGCAGGCTTTGCGTCCGTCGGGATAATCCAGcc < 2:160865/150‑1 (MQ=255) gTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTATACAAATTTCAAAGCAGGCTTTGCGTCCGTCGGGATAATCCAGCCACTGCTCAt > 1:470275/1‑150 (MQ=255) cAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACAAATTTCAAAGCAGGCTTTGCGTCCGTCGGGATAATCCAGCCACTGCTCATAgg < 2:257059/150‑1 (MQ=255) cAAGCGCACTCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCACATCAAACCGTGACGTTTCCCCACGCGGTCGTAGTACGGCACTTTACCAATTTCAAAGCAGGCTTTGCGTCCGTCGGGATAATCCAGCCACTGCTCATAgg < 1:311509/150‑1 (MQ=255) agagacagtCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACAAATTTCAAAGCAGGCTTTGCGTCCGTCGGGATAATCCAGCCACTGCTCATAgg < 1:3249/142‑1 (MQ=255) aGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACTATTTGCAAAGCAGGCTTTGGGTCCGTCGGGATAATCCAGCCACTGCTTATAGGTc < 1:44973/150‑1 (MQ=255) tCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACAAATTTCAAAGCAGGCTTTGCGTCCGTCGGGATAATCCAGCCACTGCTCATAggctgtctct > 2:3263/1‑142 (MQ=255) tCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACAAATTTCAAAGCAGGCTTTGCGTCCGTCGGGATAATCCAGCCACTGCTCATAGGTCAGTGac > 2:218078/1‑150 (MQ=255) tCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACAAATTTCAAAGCAGGCTTTGCGTCCGTCGGGATAATCCAGCCACTGCTCATAGGTCAGTGac > 1:73654/1‑150 (MQ=255) tCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACAAATTTCAAAGCAGGCTTTGCGTCCGTCGGGATAATCCAGCCACTGCTCATAGGTCAGTGaa > 1:498515/1‑149 (MQ=255) gCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACAAATTTCAAAGCAGGCTTTGCGTCCGTCGGGATAATCCAGCCACTGCTCATAGGTCAGTGACACATTATGAc < 2:73871/150‑1 (MQ=255) gCTTACGCTCGGTAATGTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACAAATTTCAAAGCAGGCTTTGCGTCCGTCGGGATAATCCAGCCACTGCTCATAGGTCAGTGACACATTATGAc < 2:301721/150‑1 (MQ=255) gTCGCGACCAAAGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACAAATTTCAAAGCAGGCTTTGCGTCCGTCGGGATAATCCAGCCACTGCTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATc > 2:591032/1‑150 (MQ=255) aaGCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACAAATTTCAAAGCAGGCTTTGCGTCCGTCGGGATAATCCAGCCACTGCTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATg < 1:217455/150‑1 (MQ=255) gCCCACCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACAAATTTCAAAGCAGGCTTTGCGTCCGTCGGGATAATCCAGCCACTGCTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGAc < 2:658922/150‑1 (MQ=255) | GCTCAGGCCGACGATACCGTTCAGCGGTGTACGCAATTCGTGACTGATGGTGGAAATAAACGTCGTTTTGTCGCGGCTGGCGCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACAAATTTCAAAGCAGGCTTTGCGTCCGTCGGGATAATCCAGCCACTGCTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGAC > REL606/3287346‑3287627 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |