Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	REL606	2,847,052	A→G	100%	V10A (GTT→GCT)	recD ←	exonuclease V (RecBCD complex), alpha chain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,847,052	0	A	G	100.0%	96.4 / NA	27	V10A (GTT→GCT)	recD	exonuclease V (RecBCD complex), alpha chain
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/14);  total (13/14)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCGGTAAACAGACATGCCCTTCCCCGGCGTCGTGGCTTAACAGGGCTGCCGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAACTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCTGCATTGGGTCGGGTTGTGTAAATCCCCTGTTGCGGATGTTCTTTATCAACGCCACGC  >  REL606/2846918‑2847194                                                                                                                                       |                                                                                                                                               gCGGTAAACAGACATGCCCTTCCCCGGCGTCGTGGCTTAACAGGGCTGCCGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCt                                                                                                                                 >  2:28179/1‑150 (MQ=255)                gCCCTTCCCCGGCGTCGTGGCTTAACAGGGCTGCCGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAACTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTa                                                                                                                  <  1:440847/150‑1 (MQ=255)                 cccTTCCCCGGCGTCGTGGCTTAACAGGGCTGCCGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTAc                                                                                                                 >  1:557365/1‑150 (MQ=255)                    ttCCCAGGCCGCGGGGCTTAACCGGGCTGCCGCGGGGCTCACGGCAGGCTGTTCACCTCCGGGCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTTCAGTATTTGCTTTTGCAATTTCATGACGcc                                                                                                              <  1:221931/150‑1 (MQ=255)                      ccccGGCGTCGTGGCTTAACAGGGCTGCCGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGcctc                                                                                                            >  2:193847/1‑150 (MQ=255)                             gtcgtGGCTTAACAGGGCTGCCGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAgg                                                                                                     >  1:474403/1‑150 (MQ=255)                              tcgtGGCTTAACAGGGCTGCCGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAggg                                                                                                    <  2:558980/150‑1 (MQ=255)                               agtGGCTTAACAGGGCTGCCGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGt                                                                                                   <  2:87675/149‑1 (MQ=255)                                 tGGCTTAACAGGGCTGCCGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTct                                                                                                 >  1:87421/1‑149 (MQ=255)                                    cTTAACAGGGCTGCCGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATAc                                                                                              <  2:260130/150‑1 (MQ=255)                                             gCTGCCGCGCGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACa                                                                                     <  1:303414/150‑1 (MQ=255)                                                gCCGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACAtct                                                                                  >  2:258065/1‑150 (MQ=255)                                                 ccGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACAtctc                                                                                 >  2:132555/1‑150 (MQ=255)                                                 ccGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACAtctc                                                                                 >  1:60459/1‑150 (MQ=255)                                                                           tctcCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCTGCAt                                                       <  1:390819/150‑1 (MQ=255)                                                                           tctcCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCTGCAt                                                       >  1:42285/1‑150 (MQ=255)                                                                           tctcCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCTGCAt                                                       >  2:41436/1‑150 (MQ=255)                                                                            ctcCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCAGCCATCAGGTCAATCAACCCTGCAtt                                                      <  1:28094/150‑1 (MQ=255)                                                                                     cGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCTGCATTGGGTCGGGt                                             <  2:42418/150‑1 (MQ=255)                                                                                     cGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCTGCATTGGGTCGGGt                                             <  2:60643/150‑1 (MQ=255)                                                                                     cGGTCAGGGCAAATTGCACAGCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCCTCCAGTAAATGCTATTGCAATTTCAGTCCGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGCCAATCAACCCTGCATTGGGTCGGGt                                             <  1:85510/150‑1 (MQ=255)                                                                                     cGGTCAGGGCAAATTGCAAATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCTGCATTGGGTCGGGt                                             <  1:480849/150‑1 (MQ=255)                                                                                                       caTCCAGCGGGCGTAGCTGTTTGTGCGCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCTGCATTGGGTGGGGTTGTGTAAATCACCTGTTg                           >  1:92547/1‑150 (MQ=255)                                                                                                           cAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCTGCATTGGGTCGGGTTGTGTAAATCCCCTGTTGCGGa                       >  2:477444/1‑150 (MQ=255)                                                                                                               gggCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAATATCTCATCCATCAGGTCAATCAACCCTGCATTGGGTCGGGTTGTGTAAATCCCCTGTTGCGGATGtt                   >  2:280853/1‑150 (MQ=255)                                                                                                                      gCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCTGCATTGGGTCGGGTTGTGTAAATCCCCTGTTGCGGATGTTCTTTATc            <  2:475752/150‑1 (MQ=255)                                                                                                                                cTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCTGCATTGGGTCGGGTTGTGTAAATCCCCTGTTGCGGATGTTCTTTATCAACGCCACGc  <  2:381392/150‑1 (MQ=255)                                                                                                                                       |                                                                                                                                               GCGGTAAACAGACATGCCCTTCCCCGGCGTCGTGGCTTAACAGGGCTGCCGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAACTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCTGCATTGGGTCGGGTTGTGTAAATCCCCTGTTGCGGATGTTCTTTATCAACGCCACGC  >  REL606/2846918‑2847194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑