Predicted mutation
evidence seq id position mutation freq annotation gene description
RA REL606 2,702,361 G→A 100% G160S (GGC→AGC)  proX → glycine betaine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,702,3610GA100.0% 154.3 / ‑6.7 46G160S (GGC→AGC) proXglycine betaine transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (24/21);  minor base C (0/1);  total (24/22)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTGGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTACGCGGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTA  >  REL606/2702218‑2702500
                                                                                                                                               |                                                                                                                                           
gcgcGGCACAGGGTTACCTGATCGATAAGAAAACCGCGGACCAGTACAAAATCACCAACATCGCAGAACTGAAAGATCCGAAGATCGCCAACCTGTACGAAACCAACGGCGACGGAAAAGCGGATTTAAACGGTTGTAACCCGAGCTggg                                                                                                                                       >  1:660275/1‑150 (MQ=255)
  agagacaggggTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGc                                                                                                                                     <  2:611903/142‑1 (MQ=255)
    ggCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCctgtctcttatac                                                                                                                                   >  1:120677/1‑143 (MQ=255)
     gCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGCTCCGAAAATCGCCAAACTGTTCGATACCAACGGCGACGGAACAGCGGATTTAACCGGTTGGAACCCTAGCTGGGGCTga                                                                                                                                  >  1:166361/1‑149 (MQ=255)
     gCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGc                                                                                                                                  >  2:564485/1‑150 (MQ=255)
          gggTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGcctgtctct                                                                                                                             >  1:610113/1‑142 (MQ=255)
          gggTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAgg                                                                                                                             >  2:218248/1‑150 (MQ=255)
          gggTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAgg                                                                                                                             >  2:386466/1‑150 (MQ=255)
          gggTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAgg                                                                                                                             >  2:448657/1‑150 (MQ=255)
          gggTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAgg                                                                                                                             >  2:245061/1‑150 (MQ=255)
          gggTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAgg                                                                                                                             >  2:571023/1‑150 (MQ=255)
          gggTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAgg                                                                                                                             >  1:489754/1‑150 (MQ=255)
          gggTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAgg                                                                                                                             >  2:599059/1‑150 (MQ=255)
          gggTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGATGTAACCCTAGCTGGGGCTGCGAAgg                                                                                                                             >  2:660141/1‑150 (MQ=255)
          gggTCACCTGATCGATAAGAAAACCGCCGACCAGGACAAAATCACCAACAGCGCACACCCGGAAGATCCGAAGACCGCAAAACTGATCGATACCCACGGCCACGGAAGAGCCGACTCAACCGGATGTAACCCTCGCACGGGCTGCCAAgg                                                                                                                             >  1:214227/1‑150 (MQ=255)
           ggTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCCCAAACATCGCAACACTGAAAGATCCGAAGATAGCCAAACTGTTAGAGACCAACGGCAAAGGAAAAGCGGAATGAACCGGTTGGAACCCTAGATGGGGTCGCGAAGGt                                                                                                                            >  1:395416/1‑150 (MQ=255)
            gTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTg                                                                                                                           <  2:581220/150‑1 (MQ=255)
                 cTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATc                                                                                                                      <  2:266573/150‑1 (MQ=255)
                       gATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAaccac                                                                                                                <  1:562845/150‑1 (MQ=255)
                        aTAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAaccacc                                                                                                               <  2:605720/150‑1 (MQ=255)
                           aGAAAACCGCCGACCAGTACAAAATAACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGc                                                                                                            <  1:467693/150‑1 (MQ=255)
                            gAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGAACCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATGTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCt                                                                                                           >  1:445185/1‑150 (MQ=255)
                                  cgccgACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCcgc                                                                                                     <  2:546869/150‑1 (MQ=255)
                                               caaaTCACCAACAGCGCCCAACTGCAAGATCCGAAGATCGCCAAACTGTTCGATACCAACCGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGAcc                                                                                        <  1:23428/149‑1 (MQ=255)
                                                  aTCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCAt                                                                                     <  1:447387/150‑1 (MQ=255)
                                                    cACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATAc                                                                                   >  2:137089/1‑150 (MQ=255)
                                                    cACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATAc                                                                                   >  2:27816/1‑150 (MQ=255)
                                                    cACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATAc                                                                                   <  1:569361/150‑1 (MQ=255)
                                                    cACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGAAACCAACGGCGACGGAAAAGCGGACTTAACCGGTTGTAACCCTAGCCGGGGCTGCGAAGGTGCGATCAACCAACAGCTTGCCGCCTATGAACTGGCCCacac                                                                                   >  1:488568/1‑150 (MQ=255)
                                                      ccAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATAcca                                                                                 <  2:446448/150‑2 (MQ=255)
                                                      ccAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCg                                                                                 <  2:166835/150‑1 (MQ=255)
                                                         aCATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGa                                                                              >  2:478896/1‑150 (MQ=255)
                                                              gCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCAt                                                                         <  2:671279/150‑1 (MQ=255)
                                                                  aaCTGAAAGATCCGAAGATCGCCAAACTGTTCGAGACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATc                                                                     <  1:386180/150‑1 (MQ=255)
                                                                      gAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAggg                                                                 <  2:32669/150‑1 (MQ=255)
                                                                            tctgaggtccgacAAACTGCGCGATACAGTCGCCGCCTGAACAGCGGGTTGAACCGGTTGTCACCCTAGCTGGGGCTGCGAAGGTGCGACCACCCACCAGCGTGCCGCGTACGAACGGACCCATACCGTGACGCATAAGGAGGGGAACTa                                                           <  1:547246/138‑1 (MQ=255)
                                                                            tCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTa                                                           <  2:491144/150‑1 (MQ=255)
                                                                                    tCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTACGCGGCga                                                   >  2:580674/1‑150 (MQ=255)
                                                                                    tCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTACGCGGCga                                                   >  2:662006/1‑150 (MQ=255)
                                                                                    tCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACCACGCGGCga                                                   >  1:505298/1‑150 (MQ=255)
                                                                                              gTTCGATACCAACGGCGACGGAAAAGCGGACTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTACGCGGCGATGATGGCCGa                                         >  2:431082/1‑150 (MQ=255)
                                                                                                 cGATACCCACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGAGCTTCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCAGACCGTGACGCATAATCAGGGGAACTACGCGGCGATGATGGCCGacac                                      <  1:477541/150‑1 (MQ=255)
                                                                                                 cGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTACGCGGCGATGATGGCCGacac                                      <  1:578974/150‑1 (MQ=255)
                                                                                                         aCGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTACGCGGCGATGATGGCCGACACCATCAGTc                              <  2:662225/150‑1 (MQ=255)
                                                                                                          cGGCGACGGAACAGCGGATTTAACCGGTTGTAACCCTCGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTGTGAGCTGACCCATACCGTGACGCATAATCAGGGGAACTACGCGGCGATGAAGGCCGACACCATCAGTCg                             <  1:360845/150‑1 (MQ=255)
                                                                                                          cGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACTGTGACGCATAATCAGGGGAACTACGCGGCGATGATGGCCGACACCATCAGTCg                             >  2:361885/1‑150 (MQ=255)
                                                                                                                  gAAAAGGGGATTTAACCGGTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGACCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTACGCGGCGATGATGGCCGACACCATCAGTCGCTACAAag                     <  1:385341/150‑1 (MQ=255)
                                                                                                                                   ggTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTACGCGGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGttt    >  2:216360/1‑150 (MQ=255)
                                                                                                                                    gTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTACGCGGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGtttt   >  2:134194/1‑150 (MQ=255)
                                                                                                                                    gTTGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTACGCGGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGtttt   >  2:562429/1‑150 (MQ=255)
                                                                                                                                     ttGTAACCCTAGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTACGCGGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTta  >  2:479094/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                           
GCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAACCGGTTGTAACCCTGGCTGGGGCTGCGAAGGTGCGATCAACCACCAGCTTGCCGCGTATGAACTGACCCATACCGTGACGCATAATCAGGGGAACTACGCGGCGATGATGGCCGACACCATCAGTCGCTACAAAGAGGGCAAACCGGTGTTTTA  >  REL606/2702218‑2702500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑