| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 1,879,026 | T→A | 100% | D380V (GAT→GTT) | yoaE ← | fused predicted membrane protein/conserved protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,879,026 | 0 | T | A | 100.0% | 54.5 / NA | 17 | D380V (GAT→GTT) | yoaE | fused predicted membrane protein/conserved protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (9/8); total (9/8) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAAGCCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACGATTCCGATGATTTCATCCAGTTCACCGCGACATACCGGGAAC > REL606/1878898‑1879166 | ggAAGTCACCCGCCACGGCTTCCAGCACAGCCAGCGGCGTGACCAAGCCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATaa < 1:462764/150‑1 (MQ=255) ttCCAGCACATCCAGCGGCGTGACCAAGCCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGc < 2:535979/150‑1 (MQ=255) aCCAAGCCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACCCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCCATCGCCGCCACATCAACGccc > 1:303821/1‑150 (MQ=255) gCCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTc > 2:413109/1‑150 (MQ=255) gCCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTc > 2:545550/1‑150 (MQ=255) gCCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTc > 2:395548/1‑150 (MQ=255) gCCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTc > 2:333487/1‑150 (MQ=255) gCCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGAAGCACGCCCAGCAGGTTGAACGGCACGAGGGTTTCCCGGACGATAATCGCCGGAGACGCCGAACCACTCGCCGCCACATCAACGCCCTCTTc > 1:140332/1‑150 (MQ=255) ccTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTcc < 1:543972/150‑1 (MQ=255) ccTTGCACCACACCAACCGCGCAGGTCACGATAACCAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGGGGGTTTCCGGGACGATAATCGCCGGGGACGCCGAAGCAATCGCCGCCACATCAACGCCCGCTTcc < 1:621997/150‑1 (MQ=255) ttGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAg > 2:122093/1‑150 (MQ=255) tGTACCAAACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAgc < 2:140734/150‑1 (MQ=255) cTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTc > 1:538337/1‑150 (MQ=255) ggTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGc < 2:304681/150‑1 (MQ=255) cGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGCGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTa > 2:433169/1‑150 (MQ=255) aCGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACGATTCCGATGATTTCATCCAGTTc < 2:261284/150‑1 (MQ=255) cGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACGATTCCGATGATTTCATCCAGTTCACCGCGAc < 1:394402/150‑1 (MQ=255) ttGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACGATTCCGATGATTTCATCCAGTTCACCGCGACATACCGGGAAc < 1:121738/150‑1 (MQ=255) | GGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAAGCCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACGATTCCGATGATTTCATCCAGTTCACCGCGACATACCGGGAAC > REL606/1878898‑1879166 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |