Predicted mutation
evidence seq id position mutation freq annotation gene description
RA REL606 1,733,865 G→T 100% A301S (GCC→TCC)  pykF → pyruvate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6061,733,8650GT100.0% 63.1 / NA 20A301S (GCC→TCC) pykFpyruvate kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (8/12);  total (8/12)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATG  >  REL606/1733723‑1734006
                                                                                                                                              |                                                                                                                                              
agaagaAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCt                                                                                                                                         <  2:76583/150‑1 (MQ=255)
    gaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGAc                                                                                                                                     >  2:191405/1‑150 (MQ=255)
    gaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGAc                                                                                                                                     <  2:657583/150‑1 (MQ=255)
            cTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGa                                                                                                                             <  2:355993/150‑1 (MQ=255)
              tCGCCCAGAAGATGATGATCTAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCTACCCAGTTGCTTGATTCCATGATCAAAAACCCACGCCCTACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACg                                                                                                                           <  1:532075/150‑1 (MQ=255)
              tCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCACATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACg                                                                                                                           <  1:81590/150‑1 (MQ=255)
                     gaagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGAt                                                                                                                    >  2:669562/1‑150 (MQ=255)
                      aagaTGATGATCGAAAAATGTATCCGTGCAAGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATg                                                                                                                   <  1:384590/150‑1 (MQ=255)
                                   aaaaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAAt                                                                                                     >  1:284462/1‑150 (MQ=255)
                                            tCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCCTGTCtcttatacacatctcc                                                                                             >  2:217013/1‑135 (MQ=255)
                                                              ttATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAg                                                                          >  2:107998/1‑150 (MQ=255)
                                                                         aCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATc                                                               <  2:480041/150‑1 (MQ=255)
                                                                             aGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATgg                                                           <  2:179005/150‑1 (MQ=255)
                                                                                                    aaCCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTaccgac                                    <  1:667593/150‑1 (MQ=255)
                                                                                                                aCTCGCGCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAAc                        <  1:190858/150‑1 (MQ=255)
                                                                                                                   cgcgCAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGc                     <  2:446863/150‑1 (MQ=255)
                                                                                                                      gcAGAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACcgctgtctcttatacaca                  >  1:223647/1‑135 (MQ=255)
                                                                                                                         gAAGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGtctc               >  2:615814/1‑150 (MQ=255)
                                                                                                                          aaGCCGGTGACGTTGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCg              <  1:107684/150‑1 (MQ=255)
                                                                                                                                      ttGCAAACTCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATg  >  2:247497/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                              
AGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATG  >  REL606/1733723‑1734006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: