Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	REL606	1,033,977	C→T	12.9%	A472T (GCC→ACC)	ycbZ ←	predicted peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,033,977	0	C	T	12.9%	76.2 / 3.8	31	A472T (GCC→ACC)	ycbZ	predicted peptidase
Reads supporting (aligned to +/- strand):  ref base C (8/19);  new base T (2/2);  total (10/21)
Fisher's exact test for biased strand distribution p-value = 5.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.26e-01

TTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAGGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATG  >  REL606/1033838‑1034123                                                                                                                                            |                                                                                                                                                   ttCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAAc                                                                                                                                          <  2:135740/150‑1 (MQ=255) ttCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAAc                                                                                                                                          <  2:189283/150‑1 (MQ=255) ttCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAAc                                                                                                                                          <  1:426099/150‑1 (MQ=255) ttCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAAc                                                                                                                                          <  1:351204/150‑1 (MQ=255) ttCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAAc                                                                                                                                          <  2:142527/150‑1 (MQ=255)   cACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTg                                                                                                                                        >  1:620219/1‑150 (MQ=255)   aagagacagaCTGTTAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGTGCGACCGAACTg                                                                                                                                        <  1:603476/141‑1 (MQ=255)   aagagacagaCTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGTGCGACCGAACTg                                                                                                                                        <  1:507846/141‑1 (MQ=255)       aGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCg                                                                                                                                    >  1:571117/1‑150 (MQ=255)         ttCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGAc                                                                                                                                  <  2:339463/150‑1 (MQ=255)            aCTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGTGCGACCGAACTgctgtctctt                                                                                                                               >  2:509295/1‑141 (MQ=255)            aCTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGTGCGACCGAACTgctgtctctt                                                                                                                               >  2:605249/1‑141 (MQ=255)               gtgAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAAcc                                                                                                                            <  2:572783/150‑1 (MQ=255)               gagAAGACCTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCGCGGATAACTCACGTTGCTGGCAAATAGCAAAGAAGCTTTCGATTTTCTCATTTAAACCACCTATCGGCTGGGCGCGACGGAACTGCTCAACTGAAcc                                                                                                                            >  1:20997/3‑150 (MQ=255)                    gACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGACAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGa                                                                                                                       >  1:582278/1‑150 (MQ=255)                         aaaTGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCg                                                                                                                  <  1:358287/150‑1 (MQ=255)                           aTGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGACAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGAt                                                                                                                <  2:583990/150‑1 (MQ=255)                                  aCGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGa                                                                                                         <  2:627046/150‑1 (MQ=255)                                   cGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGAt                                                                                                        >  2:446760/1‑150 (MQ=255)                                   cGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGAt                                                                                                        >  2:185781/1‑150 (MQ=255)                                   cGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGCCAAATAGCAAATAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGTCGCGACCGATCTGATCGACGGACACTGGGATAGCGCTACTCTGAt                                                                                                        >  1:76365/1‑150 (MQ=255)                                     ttAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTc                                                                                                      <  1:450846/150‑1 (MQ=255)                                              gggATAATGACACCTTGTCTCCCGGTTAAATCACGTTGCTGGCAAAGAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGcaca                                                                                             <  1:201709/150‑1 (MQ=255)                                                                ttCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATc                                                                           <  1:185250/150‑1 (MQ=255)                                                                  cccGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAg                                                                         <  2:411/150‑1 (MQ=255)                                                                           cTCACGTTGCTGTCAAATAGCAAAGCATCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGAGACGCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCagag                                                                <  1:554020/150‑1 (MQ=255)                                                                                 ttGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGc                                                          <  2:622037/150‑1 (MQ=255)                                                                                        cAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAg                                                   <  2:540547/150‑1 (MQ=255)                                                                                                      gCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAGGCACTATCTCCATc                                     <  1:445495/150‑1 (MQ=255)                                                                                                                      tttAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAGGCACTATCTCCATCAACTTCACTGTATGAc                     <  2:556350/150‑1 (MQ=255)                                                                                                                                      gCTTGGCACCACCGAACTGATCGACTGAACCTGTGCTAGCGGTACTCAGATTCACCGGAACATCGGCGAGGGCGCATATCAGGGCGCAGAGTTGAGACACCGAGGCCATATCTCCCTCCACTTCACTGTATGCCTGCTCAAATGTCAGCg     >  1:566587/1‑150 (MQ=255)                                                                                                                                         gggCGCGACCGAACTGATCGACTGAACCTGTGATAGCGCTGCTCTGATTCACCCGCACATCGGAGAGGCCGATTATCAGGGCGCAGAGGTAAGCCATCGAGGCACTAATCCGATCAACTTCACTTTATGATTGCTAAAATGTCCGCGATg  >  1:356017/1‑150 (MQ=255)                                                                                                                                            |                                                                                                                                                   TTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCTGTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAGGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATG  >  REL606/1033838‑1034123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑