Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	REL606	761,693	T→G	100%	noncoding (47/76 nt)	valT →	tRNA‑Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	761,693	0	T	G	100.0%	82.1 / NA	28	noncoding (47/76 nt)	valT	tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/10);  total (18/10)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACGTTGCATCAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCACTAATTACGGTGGGTTCGGTGGAAGTAGTTTGTAG  >  REL606/761550‑761835                                                                                                                                                |                                                                                                                                               aCGTTGCATCAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACAGAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGcggcgg                                                                                                                                          >  1:290375/1‑150 (MQ=255)                 ggCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCCGCGCAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTACCTTACAGGGAGTGGGGCGGCGGTTCGATCCGGtcatca                                                                                                                          >  1:156612/1‑150 (MQ=25)                 ggCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGtcatca                                                                                                                          >  2:511832/1‑150 (MQ=255)                                  gAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGtt                                                                                                         >  2:143392/1‑150 (MQ=255)                                                cTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTag                                                                                           >  2:210680/1‑150 (MQ=255)                                                       aCCCCCCACTAACATAGTTAGTTGTAGTATCCTGCGTAGTATTGGGTGATTAGCTCAGCAGGGAGAGCGCCATCCTTACAATGCGGGGGGCGGGGGTTCGATGCCGTCATACCGCACCACCGGGTCGTTAGCCCAGTTGGTAGAGCAGtt                                                                                    >  1:422080/1‑150 (MQ=255)                                                       aCCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAGGGAGGGGGGCGGCGGTTCGCTCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGtt                                                                                    >  1:67445/1‑150 (MQ=255)                                                       aCCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGtt                                                                                    >  2:297988/1‑150 (MQ=255)                                                       aCCCACCACTAACATAGTTAGGTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGTAGAGCACCTCCCTTACAAGGAGGGGTGTGACGGTTCGATCCCGTCATCACCCACAACACGGCCGTCATCTCAATTgggtgagacgtt                                                                                    >  1:302893/1‑140 (MQ=17)                                                            ccacTAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACtt                                                                               >  2:73041/1‑150 (MQ=255)                                                               cTAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTa                                                                            >  2:532973/1‑150 (MQ=255)                                                               cTAACATAGTTAGTTGTAGTATCCAGCGGAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGGTCGCGGCATCACCCACCACCGGGACGTTAGCTCAGTTGGTAGAGACGTTGACTTTTa                                                                            >  1:678934/1‑150 (MQ=14)                                                                         tagttGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGt                                                                  >  1:34893/1‑150 (MQ=255)                                                                              gtagtaTCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAg                                                             >  2:144668/1‑150 (MQ=255)                                                                              gtagtaTCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAg                                                             >  2:284013/1‑150 (MQ=255)                                                                                   aTCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCTCCGTGTCGCTAGCTCAGTTGGTAGGGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCg                                                        >  1:444708/1‑150 (MQ=11)                                                                                   aTCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTCGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCg                                                        >  1:551096/1‑150 (MQ=255)                                                                                   aTCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCg                                                        >  2:373069/1‑150 (MQ=255)                                                                                      cAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAAt                                                     <  2:34993/150‑1 (MQ=255)                                                                                        gCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATcc                                                   <  2:651214/150‑1 (MQ=38)                                                                                        gCGTAGTACTGGGCGATTAGCTCAGCTGGGAGAGCGCCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCAGCGGGTCGTTGGCTCGGTTGGTAGAGGAGTTGACTTGTGATCAATTGGTCTCAGGTTCGAATcc                                                   >  1:167281/1‑150 (MQ=11)                                                                                             gtaTTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCAc                                              >  2:648029/1‑150 (MQ=25)                                                                                                ttGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGAc                                           <  2:67643/150‑1 (MQ=25)                                                                                                       aTTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCcaccac                                    <  2:552691/150‑1 (MQ=21)                                                                                                            cTCAGCTGGGCGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCACTAAtt                               <  2:65545/150‑1 (MQ=255)                                                                                                            cTCAGCTGGGAGAGCACCGCCCGTACAAGGAGGGGGGCGGCGATTCGATCACGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGTGTTGTAATCAATTGGGCGCATGTTCGAATCCTGCACGACCCACCACTAAtt                               <  1:72827/150‑1 (MQ=11)                                                                                                              cagcagGGAGAGCGCGTCCCTTGACAGGAGGGGCGCGCCGGTTCGATCCCCTCATCCCCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCACTAATTAc                             <  1:510376/150‑1 (MQ=14)                                                                                                               aGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCACTAATTACg                            >  2:147492/1‑150 (MQ=255)                                                                                                                          cACCTCCCTTACGAGGAGGGGGGCGGCGGTTCCATCCCGTCATCACCCACCCCCGGGTCGTTAGCTCAGTTGGTATAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCACTAATTACGGTGGGTTCGGt                 <  1:142981/150‑1 (MQ=17)                                                                                                                            ccTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCACTAATTACGGTGGGTTCGGTgg               <  2:665637/150‑1 (MQ=255)                                                                                                                                  ttACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCACTAATTACGGTGGGTTCGGTGGAagtag         <  2:167757/150‑1 (MQ=255)                                                                                                                                         gaggGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCACTAATTACGGTGGGTTCGGTGGAAGTAGTTTgtag  <  2:662300/150‑1 (MQ=255)                                                                                                                                                |                                                                                                                                               ACGTTGCATCAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCACTAATTACGGTGGGTTCGGTGGAAGTAGTTTGTAG  >  REL606/761550‑761835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑