Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	REL606	3,981,782	IS150 (–) +3 bp	100%	coding (249‑251/1224 nt)	ECB_03710 ←	putative permease

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	3893554 =	NA (NA)	24 (0.900)	17/278	NT	100%	noncoding (1/1443 nt)	IS150	repeat region
	?	REL606	3981782 =	0 (0.000)					coding (251/1224 nt)	ECB_03710	putative permease
*	?	REL606	= 3894996	NA (NA)	36 (1.360)	24/276	NT	100%	noncoding (1443/1443 nt)	IS150	repeat region
	?	REL606	= 3981784	0 (0.000)					coding (249/1224 nt)	ECB_03710	putative permease

CATCATCAGTTGTGAAGTAGTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/3893670‑3893554 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTTTCGCCAAACGAATATTTCACAATGCCATACGTTGGCAGGCGGGCTTTTTCTCCCACGCTAATGGTGATGGCTGAAAGTAAGGTCAGAATGACGCCGCCTAACAATACGACGCTAATTAAACTGC  >  REL606/3981782‑3981908                                                                                                                                                                                                                                                      agagacagGTTGTGAAGTAGTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTTTCGCCAAACGAATATTTCACAATGCCATAC                                                                                                <  2:158352/142‑1  ATCATCAGTTGTGAAGTAGTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTTTCGCCAAACGAATATTTCACAATGCCATACG                                                                                               <  2:138956/150‑1  ATCATCAGTTGTGAAGTAGTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATCTTCGCCAAACGAATATTTCACAATGCCATACG                                                                                               <  1:214918/150‑1  ATCATCAGTTATGTAGTAGTGAGTCACTACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGTAGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTTTCGCCAAACGAATATTTCACATTGCCATACG                                                                                               <  1:113454/150‑1   ataagagacagGAAGTAGTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTTTCGCCAAACGAATATTTCACAATGCCATACGT                                                                                              <  1:224856/139‑1         GTTGTGAAGTAGTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTTTCGCCAAACGAATATTTCACAATGCCATACctgtctct                                                                                        >  1:157907/1‑142              GAAGTAGTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTTTCGCCAAACGAATATTTCACAATGCCATACGTctgtctcttat                                                                                   >  2:225498/1‑139                 GTAGTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTTTCGCCAAACGAATATTTCACAATGCCATACGTTGGCAGGCGGGCTT                                                                                >  1:462851/1‑150                   AGTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTTTCGCCAAACGAATATTTCACAATGCCATACGTTGGCAGGCGGGCTTTT                                                                              >  2:196368/1‑150                       ATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAAC‑AAATGGGTGCAGTACATTTTCGCCAAACGAATATTTCCCAATGCCATACGTTGGCAGGCGGGCTTTTTCTCC                                                                         >  1:170282/1‑150                                   agagacagTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTTTCGCCAAACGAATATTTCACAATGCCATACGTTGGCAGGCGGGCTTTTTCTCCCACGCTAATGG                                                              <  2:124563/142‑1                                   AAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTTTCGCCAAACGAATATTTCACAATGCCATACGTTGGCAGGCGGGCTTTTTCTCCCACGCTAATGG                                                              <  2:599063/150‑1                                   AAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTTTCGCCAAACGAATATTTCACAATGCCATACGTTGGCAGGCGGGCTTTTTCTCCCACGCTAATGG                                                              <  2:409404/150‑1                                       CGCTTTTCCAAAGGGTAATTTGTCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTTTCGCCAAACGAATATTTCACAATGCCATACGTTGGCAGGCGGGCTTTTTCTCCCACGCTAATGGTGAT                                                          <  1:487612/150‑1                                           TTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTTTCGCCAAACGAATATTTCACAATGCCATACGTTGGCAGGCGGGCTTTTTCTCCCACGCTAATGGctgtctct                                                      >  1:124198/1‑142                                                 AAGGGTATTTTGGCTTTGACATATTATGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTTTCGCCAAACGAATATTTCACAATGCCATACGTTGGCAGGCGGGCTTTTTCTCCCACGCTAATGGTGATGGCTGAAAGT                                                <  1:410330/150‑1                                                            GGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACTAAATGGGTGCAGTACATTTTCGCCAAACGTATATTTCACAATGCCATACGTTGGCAGGCGGGCTTTTTCTCCCACGATAATGGTGATGGCTGAAAGTAAGGTCAGAAT                                     <  1:135280/150‑1                                                                   ACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTTTCGCCAAACGAATATTTCACAATGCCATACGTTGGCAGGCGGGCTTTTTCTCCCACGCTAATGGTGATGGCTGAAAGTAAGGTCAGAATGACGCCG                              >  2:273406/1‑150                                                                    CATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTTTCGCCAAACGAATATTTCACAATGCCATACGTTGGCAGGCGGGCTTTTTCTCCCACGCTAATGGTGATGGCTGAAAGTAAGGTCAGAATGACGCCGC                             >  2:474814/1‑150                                                                            GGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTTTCGCCAAACGAATATTTCACAATGCCATACGTTGGCAGGCGGGCTTTTTCTCCCACGCTAATGGTGATGGCTGAAAGTAAGGTCAGAATGACGCCGCCTAACAAT                     >  1:491636/1‑150                                                                            GGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTTTCCCCAAACGAATATTTCACTATGACATACGTTGGCAGGCGGGCTTTTTCTCCCACGCTTAGTGTGATGGACGTAAGTAAGGTCAGATTGACGCCGCCTAACAAT                     >  1:288489/1‑150                                                                             GGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACATTTTCGCCAAACGAATATTTCACAATGCCATACGTTGGCAGGCGGGCTTTTTCTCCCACGCTAATGGTGATGGCTGAAAGTAAGGTCAGAATGACGCCGCCTAACAATA                    >  2:385338/1‑150                                                                                              GTCCAACAAAATGGGTGCAGTACATTTTCGCCAAACGAATATTTCACAATGCCATACGTTGGCAGGCGGGCTTTTTCTCCCACGCTAATGGTGATGGCTGAAAGTAAGGTCAGAATGACGCCGCCTAACAATACGACGCTAATTAAACTG   >  2:595311/1‑150                                                                                               TCCAACAAAATGGGTGCAGTACATTTTCGCCAAACGAATATTTCATAATGCCATACGTTGGCAGGCGGGCTTTTTCTCCCACGCTAATGGTGATGGCTGAAAGTAAGGTCAGAATGACGCCGCCTAACAATACGACGCTAATTAAACTGC  <  2:464168/150‑1                                                                                                                                                                                                                                                      CATCATCAGTTGTGAAGTAGTGATTCACGACTTCAAGGCGCTTTTCAAAAGGGTATTTTGGCTTTGACATATTAGGGGCTATTCCATTTCATCGTCCAACAAAATGGGTGCAGTACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/3893670‑3893554 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTTTCGCCAAACGAATATTTCACAATGCCATACGTTGGCAGGCGGGCTTTTTCTCCCACGCTAATGGTGATGGCTGAAAGTAAGGTCAGAATGACGCCGCCTAACAATACGACGCTAATTAAACTGC  >  REL606/3981782‑3981908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.