Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6063,357,0160G→T63.3% 94.6146intergenic (‑349/‑125)rrsD/yrdA16S ribosomal RNA/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/80):  ref base (9/38):  total (11/135)

Only 100 of 169 total aligned reads displayed.

TGCACAAGTATTTTTTTGA.TC.TTT.TT.T.TC.TGT.TTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACA  >  REL606/3356982‑3357051
                                         |                                   
tGCACAAGTATTTTTTTGA.TC.TTT.TT.T.TC.tgt.ttgt                                    <  1:313966/36‑1 (MQ=255)
 gCACAAGTATTTTTTTGA.TC.TTT.TT.T.TC.tgt.ttgtt                                   <  1:1120976/36‑1 (MQ=255)
 gCACAAGTATTTTTTTGA.TC.TTT.TT.T.TC.tgt.ttgtt                                   >  2:480454/1‑36 (MQ=255)
 gCACAAGTATTTTTTTGA.TC.TTT.TT.T.TC.tgt.ttgtt                                   >  1:1955608/1‑36 (MQ=255)
  cacaAGTATTTTTTTGA.TC.TTT.TT.T.TC.TGT.ttgttg                                  <  2:1861217/36‑1 (MQ=255)
    cttgTATTTTTTTCG.TT.TTT.TT.T.CT.TCT.TTgttgtt                                <  2:3069004/33‑1 (MQ=2)
    cgaGTATTTTTTTGA.TC.TTT.TT.T.TC.TGT.TTgttgtt                                <  1:2529049/34‑1 (MQ=255)
    caAGTATTTTTTTGA.TC.TTT.TT.T.TC.TGT.TTgttgtt                                >  2:2964271/1‑36 (MQ=255)
    caAGTATTTTTTTGA.TC.TTT.TT.T.TC.TGT.TTgttgtt                                >  2:150308/1‑36 (MQ=255)
     ttttATCTTTTTTT.TC.TTT.TT.T.TC.T.T.TTTTTGtttt                              <  2:3432939/33‑1 (MQ=2)
     ttgTATTTTTTTCT.TT.TTT.TT.T.TT.TTT.TTCTTTttt                               <  2:3166042/34‑1 (MQ=2)
     tgttATATTTTCTA.TC.TTT.TT.T.T..TTT.TTTTTGtttt                              <  2:3020135/33‑1 (MQ=9)
     taGTTTTTTTTTG..TT.TTT.TT.T.TC.TTT.TTTTTTtttt                              <  2:3725943/35‑1 (MQ=2)
     gaGTATTTTTTGGT.TT.TTT.TT.T.TT.TTT.TTTTTTttt                               <  1:2819957/35‑1 (MQ=2)
     ctttATTTTTTTGT.TT.TTT.TT.T.TT.TCT.TTTTTTttt                               <  2:3309762/33‑1 (MQ=0)
     caGTATTTTTCTGT.TT.TTT.TT.T.TT.TTT.TTTTTTttt                               <  1:3579472/35‑1 (MQ=2)
     atttATTTTTCTTT.TC.TTT.TT.T.TC.TTT.TTTTTTttt                               <  2:3068967/33‑1 (MQ=2)
     aaGTATTTTTTTGA.TC.TTT.TT.T.TC.TGT.TTGTTGttt                               >  2:3378355/1‑36 (MQ=255)
      tttATTTTTTTGT.TT.TTG.TT.T.TG.TTT.TTTTTTtttt                              <  2:3635176/34‑1 (MQ=2)
      tttATTCTTTAGT.TC.TTT.TT.T.TA.TTT.TTTTTTtttt                              <  1:2780546/34‑1 (MQ=2)
      gttATTTTTTTGT.TT.ATT.TT.T.TT.TTT.TTTTTTtttt                              <  2:2960734/34‑1 (MQ=9)
       ttttttttttTA.TA.TTT.TT.T.TC.TAT.TTGTTTttttt                             <  2:3599358/33‑1 (MQ=12)
       ttttttttgtGT.TT.TGT.TT.T.TT.TGT.TTGTTGttttt                             <  2:2841205/33‑1 (MQ=9)
       ttgtttttttTT.TC..TT.TT.T.TC.TGT.TTCTTTtttttt                            <  2:3525773/33‑1 (MQ=2)
       ttgtttttttTA.TC.TTT.TT.T.TC.TTT.TTTTTTttttt                             <  2:3460934/33‑1 (MQ=12)
       ttctttttttG..TT.TTT.TT.G.TC.TTT.TTTTTTtttttt                            <  2:3243433/33‑1 (MQ=0)
       ttcttttattGT.TT.TTT.TT.T.TT.TGT.TTTTTTttttt                             <  2:3690089/33‑1 (MQ=2)
       tattttttttGG.TT.TTT.TT.T.TC.TGT.TTTTTCctttt                             >  2:2443900/4‑36 (MQ=0)
       gTATTTTTTTCA.TC.TTT.TT.T.TC.TGT.TTGTTGttttt                             >  2:2030423/1‑36 (MQ=255)
       ctttttttttTC.TC.TTT.TT.T.T..TCT.TTTTTTtttttt                            <  2:3507277/33‑1 (MQ=2)
       ctttttgtttGA..C.TTC.TT.T.TC.TTT.TTTTTTtttttt                            <  2:3347774/33‑1 (MQ=2)
       atttttttttGT.TT.TTT.TA.T.TT.TGT.TTTTTTttttt                             <  2:3699113/33‑1 (MQ=9)
        tttttttttGT.TC.TTT.TT.T.TT.TTT.TTATTTcttttt                            <  2:3233956/34‑1 (MQ=2)
        tttttttttGT.TC.TTT.TA.T.T..TTT.TTGTTTttttttt                           <  2:3571376/34‑2 (MQ=0)
        ttttttttaGT.TC.TTT.TT.T.TT.TTT.GTTTTTtttttt                            <  1:68746/34‑1 (MQ=0)
        tttttatttTA.TT.TTT.TT.T.TC.TTT.TTTTTGtttttt                            <  2:3291089/34‑1 (MQ=2)
        tgtttttttGT.TT.TTT.TT.G.TT.TTT.TTTTTGtttttt                            <  2:3534823/34‑1 (MQ=2)
        tgtttttttG..TT.TTT.TG.T.TT.TGT.TTTTTTttttttt                           <  1:3349340/34‑2 (MQ=12)
        tgtttttttCT.TG.TGT.TT.T.TT.TGT.TTTTTGttgttt                            <  2:3337638/34‑1 (MQ=2)
        tgttttcttTA.TCTTTT.TT.T.TC.TAT.TTCTTTttttt                             <  2:3681032/34‑1 (MQ=0)
        tgtttatttGT.TT.TTT.TT.T.TT.TCT.TTTTTTtttttt                            <  2:3725818/34‑1 (MQ=2)
        tctttttttTT.TC.TTT.TT.T.T..TTT.TTATTAttttttt                           <  2:3049533/34‑2 (MQ=0)
        tctttttttTT.TC.TTT.TC.T.TT.TTT.TTCTTTtttttt                            <  1:2770592/34‑1 (MQ=2)
        tcttttgttTA.TC.TTT.TT.T.TT.TTT.TTTTTTtttttt                            <  1:3451911/34‑1 (MQ=2)
        tATTTTGTTTT.TT.TTT.TT.T.TC.TTT.TTTTTCtttttt                            <  1:2869467/36‑1 (MQ=2)
        tATTTTATTTT.TC.TTT.TT.T.TT.TTT.TTTTTCtttttt                            <  1:3561323/36‑1 (MQ=0)
        tATTATTTTG..TT.TTT.TT.T.TT.TTT.TTGTTGttttttt                           <  2:2791315/36‑2 (MQ=2)
        gttttttttGT.TT.TTT.GT.T.TT.GTT.TTTTTGtttttt                            <  2:3553093/34‑1 (MQ=2)
        gtttttcttGA.TC.TTT.TT.T.TT.TTT.TTTTTTtttttt                            <  1:3730625/34‑1 (MQ=9)
        gaTTTTGTTTT.TC.TTT.TT.T.TT.TTT.TTTTTTtttttt                            <  2:2778035/35‑1 (MQ=0)
        cttttttttTG.TT.TTT.CT.T.TC.T.T.TTGTTTttttttt                           <  2:3608489/34‑2 (MQ=2)
        ctttttcttGT.TG.TTT.TT.T.TT.TTT.TTCTTTtttttt                            <  2:2870397/34‑1 (MQ=2)
        cttttatttTA.TT.TTT.TT.T.TT.TGT.TTTGTTtttttt                            <  2:3224270/34‑1 (MQ=2)
        cctttttttCT.TC.TTT.TT.T.TT.TTT.TTTTTTtttttt                            <  2:3328767/34‑1 (MQ=2)
        caTTTTTTTCT.TC.TTTCTT.T.TT.TTT.TTTTTGttttt                             <  2:3498076/35‑1 (MQ=2)
         ttttttttGA.TG.TTT.TTCT.TT.TCT.TTTTTTtttttt                            <  1:3405735/35‑1 (MQ=0)
         ttttttttCT.TT.TTT.TT.T.TT.TGTGTTTTTGtttttt                            <  2:3127379/35‑1 (MQ=2)
         ttttttgtGT.TT.TGT.TT.T.TT.TGT.TTTTTTttttttt                           <  2:3214593/35‑2 (MQ=2)
         tgttttgtGT.TT.TTT.TT.T.TG.TTT.TTGTTTttttttt                           <  1:2760573/34‑2 (MQ=2)
         tcttttttTT.TC.TTT.TT.T.T..TGT.CTGTTTGTTTTTct                          <  2:3717029/34‑2 (MQ=0)
         gtttttttCT.TT.TTT.TT.T.TC.TTT.TTTCTCttttttt                           <  2:3272078/35‑2 (MQ=0)
         ctttttttGT.TC.TTT.TT.T.TT.TTT.TCTTTTttttttt                           <  2:3000438/35‑2 (MQ=2)
         ctttatttGT.TT.TTT.TT.T.GT.TGT.TTTTTTttttttt                           <  2:3543707/35‑2 (MQ=2)
         ccttttttGT.TT.TTT.TT.T.TT.TGT.TTTTTTttttttt                           <  1:3668973/34‑2 (MQ=2)
         aTTTTTTTGA.TT.GTT.TT.T.TT.TTT.TCTTTCttttttt                           <  2:3414295/36‑2 (MQ=2)
          tttttttTT.TT.TTG.TT.T.TCTTTT.TTGTTGttttttt                           <  2:3039719/36‑2 (MQ=2)
          tttttttTT.TC.TTT.TT.T.TT.CTT.TTCTTTtttttttc                          <  2:3570510/36‑3 (MQ=2)
          tttttttTG.TT.TTT.TT.T.TC.TTT.TTGTTCTTTTTTct                          <  2:3338294/36‑2 (MQ=12)
          tttttttGT.TT.TTT.TT.T.TT.TTT.TTCTTTTTTCTTct                          <  2:2910943/36‑2 (MQ=2)
          tttttttGT.TT.TTT.TG.T.TT.TTT.TTCTTTTTTTTTct                          <  2:3690005/36‑2 (MQ=0)
          tttttttGT.TT.TTG.TT.G.TT.TTT.TTTTTGtttttttt                          <  2:2750882/36‑3 (MQ=2)
          tttttttGC.TT.TTT.TTCT.TC.T.T.TTGTTTtttttttt                          <  2:2959147/36‑3 (MQ=2)
          tttttttGA.TT.TTT.TT.TGTC.TTT.TTTTTTttttttt                           <  1:3615383/36‑2 (MQ=9)
          tttttttGA.TC.TTT.TT.T.TC.TGT.TTGTTGTTTTTTCa                          <  2:982294/36‑1 (MQ=255)
          tttttttGA.T..TTT.TT.T.TC.TTT.TTTTTCttttttttt                         <  2:3498009/36‑4 (MQ=9)
          ttttttcTA.TT.TTT.TT.T.TT.TCT.TTCTTTtttttttt                          <  2:3507220/36‑3 (MQ=2)
          ttttttaGT.TT.TGT.TT.T.TT.TTT.TTATTGtttttttt                          <  2:3752084/36‑3 (MQ=9)
          tttttgtGT.TT.TTT.TT.T.TC.TTT.CTTTTTtttttttt                          <  2:2920789/36‑3 (MQ=2)
          ttttgttGA.TG.TTT.TT.T.TT.TTT.CTTTTTtttttttt                          <  2:3734494/36‑3 (MQ=2)
          gtttgttGC.TT.TTG.TT.T.TT.TCT.TTGTTGtttttttt                          <  2:3423338/35‑3 (MQ=0)
          attttttTC.TT.TTT.TT.T.TT.TGT.TTTTTCtttttttt                          <  2:3010287/35‑3 (MQ=9)
           ttttttTT.TT.TTT.TT.T.TT.TTT.TTGTTTTTTTTGCaa                         <  2:2937082/36‑2 (MQ=2)
           ttttttTT.TA.TTT.TT.T.TC.TGT.TTTTATttttttttt                         <  2:3039745/36‑4 (MQ=2)
           ttttttTC.TT.TTT.GT.T.TC.TTT.TTGTTTttttttttt                         <  2:3127276/36‑4 (MQ=0)
           ttttttGT.TT.TTT.TT.T.TT.CTT.TTTTTGttttttttc                         <  2:3507124/36‑4 (MQ=9)
           ttttttGG.TG.TTT.TT.T.TT.TTT.TTGTTTtttttttgt                         <  2:202943/36‑4 (MQ=2)
           ttttttGA.TT.CCT.TT.A.TT.TGT.TTTTTGttttttttg                         <  2:3020160/36‑4 (MQ=2)
           tttcttGT.TG.TTT.TT.T.TT.TGT.TTTTTTttttttttt                         <  2:3281766/36‑4 (MQ=2)
           ctttttGAGTT.TTT.TT.T.TT.TTT.TTGTTGtttttttt                          <  2:2888885/35‑3 (MQ=2)
            tttttGA.TC.TTT.TT.T.TC.TGT.TTGTTGTTTTTTCAcc                        >  2:842307/1‑36 (MQ=255)
                tGA.TC.TTT.TT.T.TC.TGT.TTGTTGTTTTTTCACCCttt                    >  1:2114620/1‑36 (MQ=255)
                tGA.TC.TTT.TT.T.TC.TGT.TTGTTGTTTTTTCACCCttt                    <  2:1467007/36‑1 (MQ=255)
                        tt.tt.t.tC.TGT.TTGTTGTTTTTTCACCCTTTTtgttg              >  1:294242/1‑36 (MQ=255)
                        tt.tt.t.tC.TGT.TTGTTGTTTTTTCACCCTTTTtgctg              <  2:3134578/36‑1 (MQ=255)
                              t.tC.TGT.TTGTTGTTTTTTCACCCTTTTTGCTGCAAt          <  1:2541083/36‑1 (MQ=255)
                                     t.ttgttGTTTTTTCACCCTTTTTGCTGCAATCGcac     <  1:2020032/36‑1 (MQ=255)
                                     t.ttgttGTTTTTTCACCCTTTTTGCTGCAATCGcac     <  1:1166855/36‑1 (MQ=255)
                                      tgtgttgtTTTTTCACCCTTTTTGCTGCAATCGcac     <  1:1727893/34‑1 (MQ=255)
                                         gttgttTTTTCACCCTTTTTGCTGCAATCGcacaca  <  1:3208967/36‑1 (MQ=255)
                                         gttgttTTTTCACCCTTTTTGCTGCAATCGcacaca  <  1:975752/36‑1 (MQ=255)
                                         |                                   
TGCACAAGTATTTTTTTGA.TC.TTT.TT.T.TC.TGT.TTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACA  >  REL606/3356982‑3357051

Base quality scores:  ATCG  < 3 ≤  ATCG  < 5 ≤  ATCG  < 8 ≤  ATCG  < 18 ≤  ATCG  < 32 ≤  ATCG