Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6064,462,9950G→T42.1% 163.9212233yjgNconserved inner membrane protein
Reads supporting (aligned to +/- strand):  new base (0/80):  ref base (28/82):  total (28/184)

Only 100 of 214 total aligned reads displayed.

TTATGGAATTACTGGTGGGAATGTTT.TTTTTA.GTTG.T.CT.TG.T.TT.TTGTTTTTTTCTATTTCGCAATATTAA  >  REL606/4462960‑4463030
                                     |                                         
ttATGGAATTACTGGTGGGAATGTTT.TTTTTA.GTTg                                           <  1:3479190/36‑1 (MQ=255)
    ggAATTACTGGTGGGAATGTTT.TTTTTA.GTTG.T.Ct.t                                    >  2:1705392/1‑36 (MQ=255)
      aaTTACTGGTGGGAATGTTT.TTTTTA.GTTG.T.Ct.tg.t                                 <  1:2088294/36‑1 (MQ=255)
        ttACTGGTGGGAATGTTT.TTTTTA.GTTG.T.Ct.tg.t.tt                              <  2:1861329/36‑1 (MQ=255)
        ttACTGGCGGGAATGTTT.TTTTTA.GTTG.T.Ct.tt.t.tt                              >  2:918333/1‑36 (MQ=37)
          aCTGGTGGGAATGTTT.TTTTTA.GTTG.T.Ct.ct.t.tt.tt                           >  1:1484458/1‑36 (MQ=37)
           cTGGTGGGAATGTTT.TTTTTA.GTTG.T.Ct.tg.t.tt.tag                          >  1:1564819/1‑34 (MQ=255)
            tggtggGAATGTTT.TTTTTA.GTTG.T.Tt.tg.t.tt.ttgt                         >  1:453135/1‑36 (MQ=255)
             ggtggGAATGTTT.TTGTTA.GTTG.T.Ct.tg.t.tt.ttgtt                        <  2:2616903/36‑1 (MQ=255)
              gtggGAATGTTT.TTTTTA.GTTG.T.Ct.tg.t.tt.ttgttt                       >  2:1585412/1‑36 (MQ=255)
                 cttaTGTTT.TTTTTT.GTTT.T.TT.TT.T.TT.TTTtttttt                    <  2:3470249/33‑1 (MQ=2)
                 cttaTGTTT.TTTTT..TTTT.T.CT.TG.T.TT.TTTttttttt                   <  2:3078838/33‑1 (MQ=12)
                 atgaTGTTT.TTTTT..TTTT.T.CT.TT.T.TT.TTTttttttt                   <  2:3488424/33‑1 (MQ=0)
                  ttttGTTT.TTTTTC.TTTG.C.TT.TT.T.TT.TTAttttttt                   <  2:3525775/33‑1 (MQ=0)
                  ttctGTTT.TTTTTT.GTTT.T.TT.TT.T.CT.TTTttttttt                   <  1:3265404/33‑1 (MQ=0)
                  ttaTGTTT.TTTTT..TTTC.T.CT.TT.T.TT.TTTtttttttt                  <  1:3103366/34‑2 (MQ=0)
                  tgttGTTT.TTTTTT.GTTT.T.TTGTG.T.TT.TTTtttttt                    <  2:3028801/33‑1 (MQ=2)
                  tgttGTTT.TTTTTT.GTGG.T.TT.TT.T.TT.TTTctttttt                   <  1:2908813/33‑1 (MQ=0)
                  tggtGTTT.TTTTTT.CTTT.T.TT.TT.T.TT.TTTttttttt                   <  1:3713311/33‑1 (MQ=2)
                  tcttGTTT.TTTTCT.GTTT.T.TT.TT.T.TT.TTTttttttt                   <  2:2850979/33‑1 (MQ=2)
                  gtttGTTT.TTTTTT.GTTT.T.GT.TT.G.TT.TTTttttttt                   <  2:2831084/33‑1 (MQ=0)
                  gtgtGTTT.TTTTTT.GTTT.T.TT.TT.T.TT.TTTttttttt                   <  2:3422158/33‑1 (MQ=0)
                  gtgtGTTT.TTTTTC.TTTT.T.TT.TC.T.TT.TTTttttttt                   <  1:3424159/33‑1 (MQ=0)
                  ggctGTTT.TTTTTT.TTTA.T.TT.TT.T.TT.TTTttttttt                   <  2:3356439/33‑1 (MQ=9)
                  atgtGTGT.TTTTTT.TTTT.T.TT.TG.T.TT.TTTttttttt                   <  1:3668999/33‑1 (MQ=2)
                   tttGTTT.TTTTTT.TTTG.TGTT.TG.T.TT.TTTgtttttt                   <  2:3185346/34‑1 (MQ=2)
                   tttGTTT.TTTTTT.TTTC.T.CT.T..T.TT.TTCTTTTCTTCt                 <  2:3690020/34‑1 (MQ=0)
                   tttGTTT.TTGTTT.TTTT.T.GT.TG.T.TT.TTTtttttttt                  <  2:3404819/34‑2 (MQ=12)
                   tttGTGT.TTTTTT.TTTG.T.TT.TG.T.TT.TTTtttttttt                  <  2:3107948/34‑2 (MQ=2)
                   tgtGTTT.TTTTTT.TTTT.T.TT.TC.T.TT.TTCtttttttt                  <  2:2940633/34‑2 (MQ=2)
                   tctGTTT.TTTTTATGTTT.T.TT.TT.T.TT.TTTttttttt                   <  1:3237185/34‑1 (MQ=2)
                   tctGTTT.TTTCTT.TTTT.T.TT.TT.T.TT.TTGTTTTTTTc                  <  1:2720065/34‑1 (MQ=0)
                   tcggTTT.TTTTGT.GTTT.T.TT.GT.T.TT.TTGTTTTTTTc                  <  1:3561262/33‑1 (MQ=2)
                   gttGTTT.TTGTTT.TTTT.T.TT.TG.T.TT.TTTtttttttt                  <  2:2844562/34‑2 (MQ=2)
                   gttGTTT.TTATTA.TTTT.T.CT.TG.T.TT.TTTtttctttt                  <  2:2960782/34‑2 (MQ=9)
                   ggtGTTT.TTTTTG.TTTG.T.TT.TT.T.TT.TTTtttttttt                  <  2:3644802/34‑2 (MQ=2)
                   cttGTTT.TTTTTT.GTTT.T.TT.TC.T.TT.TTTtttttttt                  <  2:3000471/34‑2 (MQ=2)
                   cgtGTCT.TTTTTT.GTTT.T.TT.TG.T.TT.TTTtttttttt                  <  2:2771148/34‑2 (MQ=0)
                    ttGTTT.TTTTTT.GTGT.T.TT.TT.T.TG.TTGttttttttt                 <  2:3635604/35‑3 (MQ=0)
                    ttGTGT.TTTTTT.GTTT.T.TT.TC.T.TT.TTTttttttttt                 <  2:3175584/35‑3 (MQ=2)
                    ttGTGT.TTGTTT.GTGG.T.CG.TG.T.TT.TTTttttttttt                 <  2:3553026/35‑3 (MQ=2)
                    gtGTTT.TCTTTT.GTTT.T.TT.TGTT.TT.TTGtttttttt                  <  2:3117653/35‑2 (MQ=0)
                    aTGTTTCTTTTTT.TTTG.A.TT.TG.T.TT.TTTtttttttt                  <  2:2730392/36‑2 (MQ=9)
                    aTGTTT.TTTTTT.TTTG.T.TG.TT.T.TT.TTGttttttttt                 <  2:3261942/36‑3 (MQ=9)
                    aTGTTT.TTTTTA.GTTG.T.CT.TG.T.TT.TTGTTTTTTTCt                 <  1:2552385/36‑1 (MQ=255)
                     tGTTT.TTTTTT.TTTT.T.CT.TT.T.CT.TTCtttttttttt                <  1:3624370/36‑4 (MQ=2)
                     tGTTT.TTTTTT.TTTT.T.AT.TG.T.TT.TTTtgtttttttt                <  2:3214563/36‑4 (MQ=0)
                     tGTTT.TTTTTT.GTT..T.TT.TG.T.TT.TTTTTTTTTTCttt               <  2:3048620/36‑3 (MQ=0)
                     tGTTT.TTTTTG.GTTT.T.GT.TT.T.TT.TTTtttttttttt                <  2:3500240/36‑4 (MQ=0)
                     tGTTT.TTTTCT.TTTG.T.CT.TT.T.GT.TTTttttttttgt                <  1:3170156/36‑4 (MQ=9)
                     tGTTT.TTTTAT.TTTT.T.TT.TGTT.TT.TTGttttttttt                 <  2:3562420/36‑3 (MQ=0)
                     tGTTT.TTGTCT.GTTG.T.CT.TT.T.TT.TTTcttttttttt                <  2:3496340/36‑4 (MQ=2)
                     tGTTT.TGTTTT.GTT..T.TT.TG.T.TT.TT.TTTTTTTCTTtt              <  2:3634458/36‑1 (MQ=16)
                     tGTTT.GTTTTT.GGTG.T.TT.TT.A.TT.TTGtttttttttt                <  2:2870307/36‑4 (MQ=0)
                     tGTTG.TTTTTT.TTTT.T.TT.TG.T.TT.TTGtttttttttt                <  2:2746914/36‑4 (MQ=0)
                     tGTTG.TTTTTC.TTTT.T.CT.TT.T.TT.TTTttttttttct                <  2:3098276/36‑4 (MQ=0)
                      tttt.tttttT.GTTG.T..T.T..T.TT.GTGTTTTTTTTTTttt             <  2:2959544/35‑1 (MQ=2)
                      tttt.tttttA.GTTG.T.CT.TG.T.TT.TTGTTTTTTTCttt               >  2:3728621/2‑34 (MQ=255)
                      tttt.ttttgT.GTTGTT.GT.TT.T.TT.TTGTTTTTTTCtt                <  2:3580975/35‑2 (MQ=2)
                      tttt.tttctT.TTTT.T.CT.TT.T.TT.TTCTTTTTTTCttt               <  2:3525606/35‑3 (MQ=2)
                      gTTT.TTTTTATCTTT.T.TT.TT.T.TT.TTTTTTTTTTCtt                <  1:3141462/36‑2 (MQ=9)
                      gTTT.TTTTTA.GTTG.T.CT.TG.T.TT.TTGTTTTTTTCTAt               <  2:2498419/36‑1 (MQ=255)
                       ctt.tttttT.TTTG.T..T.TA.T.TT.TTGTTTTTTTTTTttt             <  2:2738074/35‑1 (MQ=0)
                        tt.tttttT.TTTG...CT.TT.T.TT.TTTTTTTTTTCTAtttt            <  2:3233796/36‑2 (MQ=2)
                        tt.tttttT.GTTT.T.GT.TT.G.TT.TTGTTTTTTTTTTttt             <  2:3268381/36‑1 (MQ=0)
                        tt.tttttG.TTTA.T.TT.TG.T.TT.TTGTTTTTTTTTTttt             <  2:2730322/36‑1 (MQ=9)
                        tt.tttttC.TTTT.T.CT.CT.T.TT.TTTTTTTTTTCTTttt             <  2:3608443/36‑1 (MQ=0)
                        gt.tttttT.GTGC.T.TT.TG.T.TT.TTTTTTTTTTTTTttt             <  2:2709220/35‑1 (MQ=12)
                        ct.tttttT.TTTT.T.TT.TG.T.TT.TTTTTTTGTTCTTttt             <  2:3375967/35‑1 (MQ=2)
                        ct.tttttT.GTTT.T.TT.TT.T.TT.TTGTTTTTTT.TAtttt            <  2:3590183/35‑2 (MQ=0)
                         tgtttttT.GTTT.T.TT.TG.T.TT.TTTATTTTTTTTTttt             <  2:3576177/34‑1 (MQ=0)
                         t.tttttT.GTCT.T.TT.TG.T.TG.TTTTTTTTTTTTAtttt            <  2:3442224/36‑2 (MQ=2)
                         t.tttttG.TTTG.T.GT.TG.T.TT.TTTTTTTTTTTTGtttt            <  2:3156306/36‑2 (MQ=9)
                         t.tttttG.GTTT.T.TT.TG.T.TT.TTCTTTTTTTTTTtttt            <  2:3290965/36‑2 (MQ=2)
                         t.tttttA.TTTT.T.AT.TG.TGTT.TTTTTTTTTTCTCttt             <  2:3376025/36‑1 (MQ=2)
                         t.ttttcT.GTTT.T.TT.TG.T.TT.TTTTTTCTTTTTAttgt            <  2:3029695/36‑3 (MQ=0)
                         t.ttttcT.GTTG.T.TT.TG.T.TT.TTTTGTTTTTTTTtttt            <  2:2841173/36‑2 (MQ=2)
                          ttgtttA.G.TG.T.GT.TG.T.TTGTTGTTTTTTTTTTTTTc            <  2:3752070/33‑1 (MQ=0)
                          gtttttT.GTTT.T.GT.TG.T.TT.TTTTTTTTTTTTTtttt            <  2:3239265/35‑2 (MQ=2)
                          gtttttT.GTT..T.CG.TG.T.TT.TTTTTTTTTTTTTttttt           <  2:3580865/35‑3 (MQ=2)
                          gtttttC.GTTT.T.TT.TT.T.TT.TTGTTTTTTTTTTtttt            <  2:2781206/35‑2 (MQ=2)
                          gattttT.GTTT.T.TT.TG.T.TT.TTTTTTTTTTTTTtttt            <  2:3717069/34‑2 (MQ=0)
                           tttttG.TTTG.T.TT.TG.T.GT.TTTTTTTTTTTTTttttt           <  2:3146543/36‑3 (MQ=0)
                           tttttG.GTTG.T.TT.TT.G.TT.TTCTTTTTTCCTTttttt           <  2:2950112/36‑3 (MQ=0)
                           tttttG.CTTG.T.TT.TT.T.TG.TTGTTTTTTTCTTttgtg           <  2:3328790/36‑4 (MQ=0)
                           tttttA.TTTG.T.TG.TT.T.TT.TTGTTTTTTTTTTttttt           <  2:3300394/36‑3 (MQ=2)
                           tttttA.GTTG.T.TC.TT.T.TT.TTGTCTTTTGTTAttttt           <  2:3137025/36‑3 (MQ=0)
                           tttttA.GTTG.T.CT.TG.T.TT.TTGTTTTTTTCTATTTct           >  2:3310157/1‑35 (MQ=255)
                           tttttA.GTTG.T.CT.TG.T.TT.TTGTTTTTTTCTATTTct           >  1:2475464/1‑35 (MQ=255)
                           ttttgA.GTTG.T.TT.TG.C.TT.TTGTTTTTTTTTTttttt           <  2:3617419/36‑3 (MQ=9)
                            ttttT.GTTG.T.CT.GT.T.TT.TTTTTTTTTTTTTtttttt          <  2:2911147/36‑4 (MQ=0)
                             tttA.GTTG.T.CT.TG.T.TT.TTGTTTTTTTCTATTTCGCa         >  2:2398218/1‑36 (MQ=255)
                              ttA.GTTG.T.CT.TG.T.TT.TTGTTTTTTTCTATTTCTCaa        >  2:3645837/1‑36 (MQ=255)
                               tA.GTTG.T.CT.TG.T.TT.TTGTTTTTTTCTATTTCGCAat       >  2:1133594/1‑36 (MQ=255)
                                a.GTTG.T.CT.TG.T.TT.TTGTTTTTTTCTATTTCTCAata      >  2:3439238/1‑36 (MQ=255)
                                  gTTG.T.CT.TG.T.TT.TTGTTTTTTTCTATTTCGCAatat     <  1:2821673/36‑1 (MQ=255)
                                  gTTG.T.CT.TG.T.TT.TTGTTTTTTTCTATTTCGCAatat     <  2:800935/36‑1 (MQ=255)
                                    tG.T.CT.TG.T.TT.TTGTTTTTTTCTATTTCGCAATATTa   <  1:610406/36‑1 (MQ=255)
                                    tG.T.CT.TG.T.TT.TTGTTTTTTTCTATTTCGCAATATTa   >  1:347989/1‑36 (MQ=255)
                                     g.T.CT.TG.T.TT.TTGTTTTTTTCTATTTCGCAATATTaa  >  2:670225/1‑36 (MQ=255)
                                     |                                         
TTATGGAATTACTGGTGGGAATGTTT.TTTTTA.GTTG.T.CT.TG.T.TT.TTGTTTTTTTCTATTTCGCAATATTAA  >  REL606/4462960‑4463030

Base quality scores:  ATCG  < 3 ≤  ATCG  < 5 ≤  ATCG  < 10 ≤  ATCG  < 22 ≤  ATCG  < 32 ≤  ATCG