Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6063,357,0000A→T56.1% 104.3163intergenic (‑333/‑141)rrsD/yrdA16S ribosomal RNA/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/77):  ref base (17/44):  total (19/144)

Only 100 of 165 total aligned reads displayed.

TAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGA.TC.T.T.T.TTT.T.C.TGT.TTGTTGTTTTTTCACCCTTT  >  REL606/3356965‑3357033
                                   |                                          
taGGGATCCCAATTTTTTGCACAAGTATTTTTTTGa                                            >  1:1510867/1‑36 (MQ=255)
     aTCCCAATTTTTTGCACAAGGATTTTTTTGA.TC.t.t.t                                   >  2:61527/1‑36 (MQ=255)
        ccAATTTTTTGCAAAAGTATTTTTTTGA.TC.t.t.t.ttt                               <  1:3412716/36‑1 (MQ=255)
          aaTTTTTTGCACAAGTATTTTTTTGA.TC.T.T.T.TTT.T.c                           <  2:2799994/36‑1 (MQ=255)
             tttttGCACAAGTATTTTTTTGA.TC.T.T.T.TTT.T.C.tgt                       >  1:3508622/1‑36 (MQ=255)
                 tGCACAAGTATTTTTTTGA.TC.T.T.T.TTT.T.C.tgt.ttgt                  <  1:313966/36‑1 (MQ=255)
                  gCACAAGTATTTTTTTGA.TC.T.T.T.TTT.T.C.tgt.ttgtt                 <  1:1120976/36‑1 (MQ=255)
                  gCACAAGTATTTTTTTGA.TC.T.T.T.TTT.T.C.tgt.ttgtt                 >  1:1955608/1‑36 (MQ=255)
                   cacaAGTATTTTTTTGA.TC.T.T.T.TTT.T.C.TGT.ttgttg                <  2:1861217/36‑1 (MQ=255)
                     cgaGTATTTTTTTGA.TC.T.T.T.TTT.T.C.TGT.TTgttgtt              <  1:2529049/34‑1 (MQ=255)
                     caAGTATTTTTTTGA.TC.T.T.T.TTT.T.C.TGT.TTgttgtt              >  2:2964271/1‑36 (MQ=255)
                     caAGTATTTTTTTGA.TC.T.T.T.TTT.T.C.TGT.TTgttgtt              >  2:150308/1‑36 (MQ=255)
                      ttttATCTTTTTTT.TC.T.T.T.TTT.T.C.T.T.TTTTTGtttt            <  2:3432939/33‑1 (MQ=2)
                      ttgTATTTTTTTCT.TT.T.T.T.TTT.T.T.TTT.TTCTTTttt             <  2:3166042/34‑1 (MQ=2)
                      gtttATTTTTTTTA.TT.T.T.T.TTC.T.T.TTT.TTGTTTttt             <  2:3488730/33‑1 (MQ=0)
                      gaGTATTTTTTGGT.TT.T.T.T.TTT.T.T.TTT.TTTTTTttt             <  1:2819957/35‑1 (MQ=2)
                      ctttATTTTTTTGT.TT.T.T.T.TTT.T.T.TCT.TTTTTTttt             <  2:3309762/33‑1 (MQ=0)
                      caGTATTTTTCTGT.TT.T.T.T.TTT.T.T.TTT.TTTTTTttt             <  1:3579472/35‑1 (MQ=2)
                      atttATTTTTCTTT.TC.T.T.T.TTT.T.C.TTT.TTTTTTttt             <  2:3068967/33‑1 (MQ=2)
                      aaGTATTTTTTTGA.TC.T.T.T.TTT.T.C.TGT.TTGTTGttt             >  2:3378355/1‑36 (MQ=255)
                       tttATTTTTTTGT.TT.T.T.G.TTT.T.G.TTT.TTTTTTtttt            <  2:3635176/34‑1 (MQ=2)
                       tgTATTTTGTTTT.TC.T.T.T.TTT.T...TGT.TTTTTTttttt           <  2:2831109/35‑1 (MQ=0)
                       gttATTTTTTTGT.TT.A.T.T.TTT.T.T.TTT.TTTTTTtttt            <  2:2960734/34‑1 (MQ=9)
                        ttttttttttGT.TA.T.T.T.TTT.T.T.TTT.CTGTTTttttt           <  2:2901269/33‑1 (MQ=2)
                        ttttttttttCT.TC.T.T.T.TTG.T.T.TTT.TTGTTTttttt           <  2:2940322/33‑1 (MQ=2)
                        ttgtttttttTT.TC...T.T.TTT.T.C.TGT.TTCTTTtttttt          <  2:3525773/33‑1 (MQ=2)
                        ttgtttttttTA.TC.T.T.T.TTT.T.C.TTT.TTTTTTttttt           <  2:3460934/33‑1 (MQ=12)
                        ttctttttttG..TT.T.T.T.TTG.T.C.TTT.TTTTTTtttttt          <  2:3243433/33‑1 (MQ=0)
                        tcttttttttGA.T..T.T.T.TCT.T.C.TTT.TNTTTTtttttt          <  2:3590180/33‑1 (MQ=2)
                        tcgtttttttG..TT.T.T.T.TTT.T.C.TTT.TTTTTTtttttt          <  2:3049520/33‑1 (MQ=2)
                        tattttttttGG.TT.T.T.T.TTT.T.C.TGT.TTTTTCctttt           >  2:2443900/4‑36 (MQ=0)
                        gTATTTTTTTCA.TC.T.T.T.TTT.T.C.TGT.TTGTTGttttt           >  2:2030423/1‑36 (MQ=255)
                        ctttttttttTC.TC.T.T.T.TTT.T...TCT.TTTTTTtttttt          <  2:3507277/33‑1 (MQ=2)
                        ctttttgtttGA..C.T.T.C.TTT.T.C.TTT.TTTTTTtttttt          <  2:3347774/33‑1 (MQ=2)
                        ctgtttttttGA.TT.T.T.T.TTT.T.T.TTT.TTTTTTttttt           <  1:2760623/33‑1 (MQ=9)
                        ctATTTTTTTG..TA.T.T.T.TTT.T.T.TTT.TTTTTTtttttt          <  1:3160678/35‑1 (MQ=0)
                        atttttttttGT.TT.T.T.T.TAT.T.T.TGT.TTTTTTttttt           <  2:3699113/33‑1 (MQ=9)
                         tttttttttGT.TC.T.T.T.TTT.T.T.TTT.TTATTTcttttt          <  2:3233956/34‑1 (MQ=2)
                         tttttttttGT.TC.T.T.T.TAT.T...TTT.TTGTTTttttttt         <  2:3571376/34‑2 (MQ=0)
                         tttttttttGT.TC.T.T.A.TTG.T.C.TTG.TTTTTTtttttt          <  2:3059305/34‑1 (MQ=2)
                         ttttttttaGT.TC.T.T.T.TTT.T.T.TTT.GTTTTTtttttt          <  1:68746/34‑1 (MQ=0)
                         tttttgtttGT.CC.T.T.T.TTT.T.C.TTT.TTGTTTtttttt          <  2:3224117/34‑1 (MQ=9)
                         tttttatttTA.TT.T.T.T.TTT.T.C.TTT.TTTTTGtttttt          <  2:3291089/34‑1 (MQ=2)
                         tgtttttttGT.TG.T.T.T.TTT.T.G.TTT.TTCTGTtttttt          <  2:2789881/34‑1 (MQ=2)
                         tgtttttttG..TT.T.T.T.TGT.T.T.TGT.TTTTTTttttttt         <  1:3349340/34‑2 (MQ=12)
                         tgtttttttCT.TG.T.G.T.TTT.T.T.TGT.TTTTTGttgttt          <  2:3337638/34‑1 (MQ=2)
                         tgttttcttTA.TCTT.T.T.TTT.T.C.TAT.TTCTTTttttt           <  2:3681032/34‑1 (MQ=0)
                         tgtttatttGT.TT.T.T.T.TTT.T.T.TCT.TTTTTTtttttt          <  2:3725818/34‑1 (MQ=2)
                         tctttttttTT.TC.T.T.T.TCT.T.T.TTT.TTCTTTtttttt          <  1:2770592/34‑1 (MQ=2)
                         tcttttgttTA.TC.T.T.T.TTT.T.T.TTT.TTTTTTtttttt          <  1:3451911/34‑1 (MQ=2)
                         tATTTTTTTTT.TC.T.T.T.TTT.T.G.TCT.GTTTTTtttttt          <  2:1504745/36‑1 (MQ=0)
                         tATTTTGTTTT.TT.T.T.T.TTT.T.C.TTT.TTTTTCtttttt          <  1:2869467/36‑1 (MQ=2)
                         gttttttttGT.TT.T.T.T.GTT.T.T.GTT.TTTTTGtttttt          <  2:3553093/34‑1 (MQ=2)
                         gttttttgtGT.TT.T.T.T.TTT.T.C.T.T.TTGTTTttttttt         <  2:3291132/34‑2 (MQ=2)
                         gtgttttttTT.TC.T.T.T.TTT.T.T.TGT.TTTTTTtttttt          <  2:3507140/33‑1 (MQ=0)
                         ctttttcttGT.TG.T.T.T.TTT.T.T.TTT.TTCTTTtttttt          <  2:2870397/34‑1 (MQ=2)
                         cggtttttcTA.TT.T.T.T.TTT.G.T.TTT.TTGTTGtttttt          <  2:3617270/33‑1 (MQ=2)
                         cctttttttCT.TC.T.T.T.TTT.T.T.TTT.TTTTTTtttttt          <  2:3328767/34‑1 (MQ=2)
                         caTTTTTTTCT.TC.T.T.TCTTT.T.T.TTT.TTTTTGttttt           <  2:3498076/35‑1 (MQ=2)
                          ttttttttTT.TT.T.T.T.TTT.T.G.TTG.TTGTTTttttttg         <  2:3078788/35‑2 (MQ=2)
                          ttttttttGT.TT.T.G.T.TGT.T.T.TGT.TGTTTGttttttt         <  2:844494/35‑2 (MQ=0)
                          ttttttttGC.TT.TCT.T.TTT.T.T.TCT.TTTTTGtttttt          <  2:3253044/35‑1 (MQ=0)
                          ttttttttCT.TT.T.T.T.TTT.T.T.TGTGTTTTTGtttttt          <  2:3127379/35‑1 (MQ=2)
                          ttttttgtGT.TT.T.G.T.TTT.T.T.TGT.TTTTTTttttttt         <  2:3214593/35‑2 (MQ=2)
                          tgttttgtGT.TT.T.T.T.TTT.T.G.TTT.TTGTTTttttttt         <  1:2760573/34‑2 (MQ=2)
                          tcttttttTT.TC.T.T.T.TTT.T...TGT.CTGTTTGTTTTTct        <  2:3717029/34‑2 (MQ=0)
                          ctttttttG..TT.T.T.T.TTT.T.C.TTT.TTTTTTTTTTTTct        <  2:3525816/35‑2 (MQ=2)
                          ctttatttGT.TT.T.T.T.TTT.G.T.TGT.TTTTTTttttttt         <  2:3543707/35‑2 (MQ=2)
                          ccttttttGT.TT.T.T.T.TTT.T.T.TGT.TTTTTTttttttt         <  1:3668973/34‑2 (MQ=2)
                          aTTTTTTTGA.TT.G.T.T.TTT.T.T.TTT.TCTTTCttttttt         <  2:3414295/36‑2 (MQ=2)
                           tttttttTT.TT.T.T.G.TTT.T.CTTTT.TTGTTGttttttt         <  2:3039719/36‑2 (MQ=2)
                           tttttttTG.TT.T.T.T.TTT.T.C.TTT.TTGTTCTTTTTTct        <  2:3338294/36‑2 (MQ=12)
                           tttttttTG.TT.T.T.T.TTG.T.T.TTT.TTGTTGttgtttgt        <  2:3020148/36‑3 (MQ=2)
                           tttttttTA.TT.T.T.G.TTT.T.C.TGT.TTTTTTtttttttt        <  2:3562518/36‑3 (MQ=2)
                           tttttttGT.TT.T.T.T.TGT.T.T.TTT.TTCTTTTTTTTTct        <  2:3690005/36‑2 (MQ=0)
                           tttttttGT.TT.T.T.G.TTG.T.T.TTT.TTTTTGtttttttt        <  2:2750882/36‑3 (MQ=2)
                           tttttttGA.TT.T.T.T.TTTGT.C.TTT.TTTTTTttttttt         <  1:3615383/36‑2 (MQ=9)
                           tttttttGA.TC.T.T.T.TTT.T.C.TGT.TTGTTGTTTTTTCa        <  2:982294/36‑1 (MQ=255)
                           tttttttCT.TC.T.T.T.TTT.T...TCT.TTATTTttttttttt       <  1:2800152/36‑4 (MQ=2)
                           tttttttAA.TT.T.T.T.TTT.TAG.TTT.TTGTTTttttttt         <  2:3743376/36‑2 (MQ=2)
                           ttttttaGT.TT.T.G.T.TTT.T.T.TTT.TTATTGtttttttt        <  2:3752084/36‑3 (MQ=9)
                           tttttgtGT.TT.T.T.T.TTT.T.C.TTT.CTTTTTtttttttt        <  2:2920789/36‑3 (MQ=2)
                           tttgtttTA.TC.T.T.T.TTT.T.T.TCT.TTTTTTtttttttt        <  2:3010165/36‑3 (MQ=0)
                           gttttttTC.TC.T.C.T.TTT.T.T.TGT.TTTTTTtttttttt        <  2:2750857/35‑3 (MQ=2)
                           gttttttGT.TC.T.T.T.TTTCT.T.TGT.TTTTTTttttttt         <  2:3020198/35‑2 (MQ=2)
                           gtttgttGC.TT.T.T.G.TTT.T.T.TCT.TTGTTGtttttttt        <  2:3423338/35‑3 (MQ=0)
                           cttttttCG.TC.T.T.T.TTT.T.T.TTT.TTTTTTtttttttt        <  2:3690010/35‑3 (MQ=0)
                           attttttTC.TT.T.T.T.TTT.T.T.TGT.TTTTTCtttttttt        <  2:3010287/35‑3 (MQ=9)
                            ttttttTT.TT.T.T.T.TTT.T.T.TTT.TTGTTTTTTTTGCaa       <  2:2937082/36‑2 (MQ=2)
                            ttttttGT.TT.T.TGT.TTT.T.C.T.T.TTGTTTttttttttt       <  2:3732058/36‑4 (MQ=2)
                            ttttttGT.TT.T.T.T.TTT.T.T.CTT.TTTTTGttttttttc       <  2:3507124/36‑4 (MQ=9)
                            ttttttGG.TG.T.T.T.TTT.T.T.TTT.TTGTTTtttttttgt       <  2:202943/36‑4 (MQ=2)
                            tttcttGT.TG.T.T.T.TTT.T.T.TGT.TTTTTTttttttttt       <  2:3281766/36‑4 (MQ=2)
                            ctttttGAGTT.T.T.T.TTT.T.T.TTT.TTGTTGtttttttt        <  2:2888885/35‑3 (MQ=2)
                             tttttGT.TT.T.G.T.TTT.T.T.TGTGTTGTTGtttttttgg       <  2:3127326/36‑4 (MQ=0)
                             tttttGT.TC.T.T.T.TTT.TGG.TGT.TTTTTCttttttttt       <  2:3608448/36‑4 (MQ=2)
                             tttttGA.TC.T.T.T.TTT.T.C.TGT.TTGTTGTTTTTTCatc      >  2:2970731/1‑34 (MQ=255)
                             tttttGA.TC.T.T.T.TTT.T.C.TGT.TTGTTGTTTTTTCAcc      >  2:842307/1‑36 (MQ=255)
                                 tGA.TC.T.T.T.TTT.T.C.TGT.TTGTTGTTTTTTCACCCttt  <  2:1467007/36‑1 (MQ=255)
                                 tGA.TC.T.T.T.TTT.T.C.TGT.TTGTTGTTTTTTCACCCttt  >  1:2114620/1‑36 (MQ=255)
                                   |                                          
TAGGGATCCCAATTTTTTGCACAAGTATTTTTTTGA.TC.T.T.T.TTT.T.C.TGT.TTGTTGTTTTTTCACCCTTT  >  REL606/3356965‑3357033

Base quality scores:  ATCG  < 3 ≤  ATCG  < 5 ≤  ATCG  < 9 ≤  ATCG  < 18 ≤  ATCG  < 32 ≤  ATCG