Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6064,463,0000G→T42.0% 175.0198238yjgNconserved inner membrane protein
Reads supporting (aligned to +/- strand):  new base (0/76):  ref base (21/84):  total (21/177)

Only 100 of 219 total aligned reads displayed.

GAATTACTGGTGGGAATGTTTTTTTTA.GTTG.TC.T.T.G.T.TT.TTGTTTTTTTCTATTTCGCAATATTAATGAC  >  REL606/4462965‑4463034
                                        |                                     
gAATTACTGGTGGGAATGTTTTTTTTA.GTTG.TC.t.t.n                                       <  2:2610636/36‑1 (MQ=255)
 aaTTACTGGTGGGAATGTTTTTTTTA.GTTG.TC.t.t.g.t                                     <  1:2088294/36‑1 (MQ=255)
   ttACTGGTGGGAATGTTTTTTTTA.GTTG.TC.t.t.g.t.tt                                  <  2:3141854/36‑1 (MQ=255)
     aCTGGTGGGAATGTTTTTTTTA.GTTG.TC.t.c.t.t.tt.tt                               >  1:1484458/1‑36 (MQ=37)
      cTGGTGGGAATGTTTTTTTTA.GTTG.TC.t.t.g.t.tt.ttg                              <  2:3267943/36‑1 (MQ=255)
      cTGGTGGGAATGTTTTTTTTA.GTTG.TC.t.t.g.t.tt.tag                              >  1:1564819/1‑34 (MQ=255)
            cttaTGTTTTTTTTT.GTTT.TT.T.T.T.T.TT.TTTtttttt                        <  2:3470249/33‑1 (MQ=2)
            cttaTGTTTTTTTT..TTTT.TC.T.T.G.T.TT.TTTttttttt                       <  2:3078838/33‑1 (MQ=12)
            atgaTGTTTTTTTT..TTTT.TC.T.T.T.T.TT.TTTttttttt                       <  2:3488424/33‑1 (MQ=0)
             ttttGTTTTTTTTC.TTTG.CT.T.T.T.T.TT.TTAttttttt                       <  2:3525775/33‑1 (MQ=0)
             ttctGTTTTTTTTT.GTTT.TT.T.T.T.T.CT.TTTttttttt                       <  1:3265404/33‑1 (MQ=0)
             tgttGTTTTTTTTT.GTTT.TT.TGT.G.T.TT.TTTtttttt                        <  2:3028801/33‑1 (MQ=2)
             tgttGTTTTTTTTT.GTGG.TT.T.T.T.T.TT.TTTctttttt                       <  1:2908813/33‑1 (MQ=0)
             tcttGTTTTTTTCT.GTTT.TT.T.T.T.T.TT.TTTttttttt                       <  2:2850979/33‑1 (MQ=2)
             gtttGTTTTTTTTG.TTTG.TT.T.T.T.T.TT.TTTttttttt                       <  2:3338190/33‑1 (MQ=2)
             gtttGTTGTTTTTT.TTTG.TT.T.T.T.T.TT.TTTttttttt                       <  2:3127329/33‑1 (MQ=0)
             ggctGTTTTTTTAT.GTTT.TT.T.T.T.T.TT.TTTttttttt                       <  2:3049489/33‑1 (MQ=0)
             caaTGTTTTTTTTT.ATTT.TT.T.T.T.T.TT.TTTttttttt                       <  1:3713303/35‑1 (MQ=9)
              tttGTTTTTTTTT.TTTC.TC.T.T...T.TT.TTCTTTTCTTCt                     <  2:3690020/34‑1 (MQ=0)
              tttGTTTTTTTTT.GTGT.TT.T.T.T.T.TT.TTGtttttttt                      <  1:3651341/34‑2 (MQ=2)
              tttGTTTTTTTTG.GGTT.TT.A.T.G.T.TT.TTTtttttttt                      <  2:3185310/34‑2 (MQ=0)
              tttGTTTTTTATC.CTTG.TT.T.T.T.T.TT.TTTtttttttt                      <  2:3290985/34‑2 (MQ=0)
              tttGTTTTTGTTT.TTTT.TG.T.T.G.T.TT.TTTtttttttt                      <  2:3404819/34‑2 (MQ=12)
              tttGTGTTTTTTT.TTTG.TT.T.T.G.T.TT.TTTtttttttt                      <  2:3107948/34‑2 (MQ=2)
              tgtGTTTTTTTTT.TTTT.TT.T.T.C.T.TT.TTCtttttttt                      <  2:2940633/34‑2 (MQ=2)
              tgtGTTTTTGTTT.GTTT.TT.T.T.T.T.TTGTTGttttttt                       <  2:3039369/34‑1 (MQ=2)
              tgtGTTTTTATCT.GTTG.TT.T.TCG.T.TT.TTGttttttt                       <  2:3644839/34‑1 (MQ=2)
              tctGTTTTTTTTATGTTT.TT.T.T.T.T.TT.TTTttttttt                       <  1:3237185/34‑1 (MQ=2)
              tctGTTTTTTCTT.TTTT.TT.T.T.T.T.TT.TTGTTTTTTTc                      <  1:2720065/34‑1 (MQ=0)
              tcggTTTTTTTGT.GTTT.TT.T.G.T.T.TT.TTGTTTTTTTc                      <  1:3561262/33‑1 (MQ=2)
              taTGTTTTTTTTT.GTCT.TT.T.T.T.T.AT.TTTtttttttt                      <  1:3151048/35‑2 (MQ=0)
              taTGTTTTTTTTT.CTT..TC.T.T.T.T.TT.TTTttttttttt                     <  1:2859545/35‑3 (MQ=0)
              gttGTTTTTGTTT.TTTT.TT.T.T.G.T.TT.TTTtttttttt                      <  2:2844562/34‑2 (MQ=2)
              gttGTTTTTATTA.TTTT.TC.T.T.G.T.TT.TTTtttctttt                      <  2:2960782/34‑2 (MQ=9)
              gttGTTTGTTTTT.TTTG.TT.T.T.T.T.TT.TTTtttttttt                      <  2:3164436/34‑2 (MQ=0)
              cttGTTTTTTTTT.GTTT.TT.T.T.C.T.TT.TTTtttttttt                      <  2:3000471/34‑2 (MQ=2)
              cgtGTGTTTTGTT.GTTT.TT.T.T.T.T.TT.TTGtttttttt                      <  2:3304987/34‑2 (MQ=0)
              cgtGTCTTTTTTT.GTTT.TT.T.T.G.T.TT.TTTtttttttt                      <  2:2771148/34‑2 (MQ=0)
              aaTGTTTTTTTTA.GTTG.TC.T.T.G.T.TT.TTGTTTTTTTc                      <  2:68791/36‑1 (MQ=255)
               ttGTTTTTTTTT.TTTT.TC.TCT.C.T.TT.TTCtttttttt                      <  1:3451909/35‑2 (MQ=2)
               ttGTTTTTTTTT.GTGT.TT.T.T.T.T.TG.TTGttttttttt                     <  2:3635604/35‑3 (MQ=0)
               ttGTTTTGTTTT.TTTT.TA.T.T.T.T.TT.TTGttttttttt                     <  2:3525280/35‑3 (MQ=0)
               ttGTGTTTGTTT.GTGG.TC.G.T.G.T.TT.TTTttttttttt                     <  2:3553026/35‑3 (MQ=2)
               ctGTTGTTTTTC.GTTT.TT.T.T.G.T.GT.TTTttttttttt                     <  1:3074505/35‑3 (MQ=0)
               aTGTTTTTTTTA.GTTG.TC.T.T.G.T.TT.TTGttttttttt                     >  1:373022/1‑34 (MQ=255)
               aTGTTTTTTTTA.GTTG.TC.T.T.G.T.TT.TTGTTTTTTTCt                     <  2:3421301/36‑1 (MQ=255)
               aTGTTTTTTTTA.GTTG.TC.T.T.G.T.TT.TTGTTTTTTTCt                     <  1:2552385/36‑1 (MQ=255)
                tGTTTTTTTTG.GTTT.TG.T.T.T.T.TT.TTTtttttttttt                    <  2:3500240/36‑4 (MQ=0)
                tGTTTTTTTAT.TTTT.TT.T.T.GTT.TT.TTGttttttttt                     <  2:3562420/36‑3 (MQ=0)
                tGTTTTTGTCT.GTTG.TC.T.T.T.T.TT.TTTcttttttttt                    <  2:3496340/36‑4 (MQ=2)
                tGTTTTGTTTT.GTT..TT.T.T.G.T.TT.TT.TTTTTTTCTTtt                  <  2:3634458/36‑1 (MQ=16)
                tGTTTGTTTTT.GGTG.TT.T.T.T.A.TT.TTGtttttttttt                    <  2:2870307/36‑4 (MQ=0)
                tGTTGTTTTTT.TTTT.TT.T.T.G.T.TT.TTGtttttttttt                    <  2:2746914/36‑4 (MQ=0)
                tGTTGTTTTTC.TTTT.TC.T.T.T.T.TT.TTTttttttttct                    <  2:3098276/36‑4 (MQ=0)
                tGTCTTTTTTT.TTTT.TT.T.T.T.T.TT.TTGttttttttct                    <  2:3243585/36‑4 (MQ=2)
                 tttttttttT.GTTG.T..T.T...T.TT.GTGTTTTTTTTTTttt                 <  2:2959544/35‑1 (MQ=2)
                 tttttttttA.GTTG.TC.T.T.G.T.TT.TTGTTTTTTTCttt                   >  2:3728621/2‑34 (MQ=255)
                 ttttttttgT.GTTGTTG.T.T.T.T.TT.TTGTTTTTTTCtt                    <  2:3580975/35‑2 (MQ=2)
                 gTTTTTTTTA.GTTG.TC.T.T.G.T.TT.TTGTTTTTTTCttt                   >  1:311559/1‑34 (MQ=255)
                  ttttttttG.TTTG.T..T.T.G.T.TT.TT.TTTTTTTCTTtttt                <  2:2730337/36‑2 (MQ=0)
                  ttttgtttT.GTT..TC.T.T.G.T.TT.TTTTTTTGTTTTTttt                 <  2:3272149/36‑1 (MQ=2)
                  gcttttttT.GTT..TT.T.T.G.T.TT.TTTTTTTTTTTTTttt                 <  2:3328129/34‑1 (MQ=0)
                  ctttttttT.TTTG.T..T.T.A.T.TT.TTGTTTTTTTTTTttt                 <  2:2738074/35‑1 (MQ=0)
                   tttttttC.TTTT.TC.T.C.T.T.TT.TTTTTTTTTTCTTttt                 <  2:3608443/36‑1 (MQ=0)
                   tttttttA.GTTG.TC.T.T.G.T.TT.TTGTTTTTTTCTAttt                 >  2:840184/1‑36 (MQ=255)
                   ttgttttT.GTTC.TT.T.T.T.T.TT.TTGTTTTTTTTTTttt                 <  1:3368036/33‑1 (MQ=0)
                   tgtttttT.GTTT.TT.T.T.G.T.TT.TTTATTTTTTTTTttt                 <  2:3576177/34‑1 (MQ=0)
                   tggttttT.GTTG.TT.T.T.G.T.TT.TTTTTTTTTTTTTttt                 <  2:3366436/33‑1 (MQ=0)
                   gttttttT.TTTG.TT.G.T.T.T.TT.TTGTTTTTTTTTTttt                 <  2:2719734/35‑1 (MQ=0)
                   gttttttG.TTTT.TG.T.T.G.T.TT.TTTTTTTTTTTTTttt                 <  2:3233770/35‑1 (MQ=0)
                   gttttttC.TTTT.TG.T.T.G.T.TT.TT.TTTTTTTCTCtttt                <  2:3233853/35‑2 (MQ=2)
                   cttttttT.GTTT.TT.T.T.T.T.TT.TTGTTTTTTT.TAtttt                <  2:3590183/35‑2 (MQ=0)
                    ttttttT.TTTC.TG.T.T.C.T.TT.TTGTTTTTTTTTCtttt                <  2:3156278/36‑2 (MQ=2)
                    ttttttT.GTTT.TG.T.G.T.T.TT.TTGTTTTTTTTTTtttt                <  2:3442072/36‑2 (MQ=0)
                    ttttttA.TTTT.TA.T.T.G.TGTT.TTTTTTTTTTCTCttt                 <  2:3376025/36‑1 (MQ=2)
                    ttttttA.GTGC.TT.T.T.G.T.TT.TTATTTATTTATTtttt                <  2:2821279/36‑2 (MQ=0)
                    tttttcT.GTTG.TT.T.T.G.T.TT.TTTTGTTTTTTTTtttt                <  2:2841173/36‑2 (MQ=2)
                    ttttatA.TTTT.TT.T.T.G.T.TT.TTTGTTTTTTTTAtttt                <  2:3194733/36‑2 (MQ=0)
                    gtttttT.GTT..TC.G.T.G.T.TT.TTTTTTTTTTTTTttttt               <  2:3580865/35‑3 (MQ=2)
                    gtttttC.GTTT.TT.T.T.T.T.TT.TTGTTTTTTTTTTtttt                <  2:2781206/35‑2 (MQ=2)
                    gattttT.GTTT.TT.T.T.G.T.TT.TTTTTTTTTTTTTtttt                <  2:3717069/34‑2 (MQ=0)
                     tttttG.GTTG.TT.T.T.T.G.TT.TTCTTTTTTCCTTttttt               <  2:2950112/36‑3 (MQ=0)
                     tttttG.GTTG.TT.G.T.T.T.TT.GTGTTTTTTTTTTttttt               <  2:3648000/36‑3 (MQ=0)
                     tttttA.GTTG.TC.T.T.G.T.TT.TTGTTTTTTTCTATTTct               >  1:2475464/1‑35 (MQ=255)
                     ttttgA.GTTG.TT.T.T.G.C.TT.TTGTTTTTTTTTTttttt               <  2:3617419/36‑3 (MQ=9)
                     cttttT.GTTT.TCTT.T.G.T.TT.TTTTTTTTTTTTTtttt                <  2:3423397/35‑2 (MQ=9)
                      ttttT.GTTG.TC.T.G.T.T.TT.TTTTTTTTTTTTTtttttt              <  2:2911147/36‑4 (MQ=0)
                       tttA.GTTG.TC.T.T.G.T.TT.TTGTTTTTTTCTATTTCGCa             >  2:2398218/1‑36 (MQ=255)
                         tA.GTTG.TC.T.T.G.T.TT.TTGTTTTTTTCTATTTCGCAat           >  2:1133594/1‑36 (MQ=255)
                          a.GTTG.TC.T.T.G.T.TT.TTGTTTTTTTCTATTTCGCAata          <  2:162197/36‑1 (MQ=255)
                             ttG.TC.T.T.G.T.TT.TTGTTTGTTTCTATTTCGCAATAtt        <  2:2683406/36‑1 (MQ=255)
                              tG.TC.T.T.G.T.TT.TTGTTTTTTTCTATTTCGCAATATTa       >  1:347989/1‑36 (MQ=255)
                              tG.TC.T.T.G.T.TT.TTGTTTTTTTCTATTTCGCAATATTa       <  1:610406/36‑1 (MQ=255)
                                 tC.T.T.G.T.TT.TTGTTTTTTTCTATTTCGCAATATTAAt     >  1:3607742/1‑36 (MQ=255)
                                 tC.T.T.G.T.TT.TTGTTTTTTTCTATTTCGCAATATTAAt     <  1:956878/36‑1 (MQ=255)
                                 tC.T.T.G.T.TT.TTGTTTTTTTCTATTTCGCAATATTAAt     >  2:55150/1‑36 (MQ=255)
                                  c.T.T.G.T.TT.TTGTTTTTTTCTATTTCGCAATATTAATg    >  2:926368/1‑36 (MQ=255)
                                  c.T.T.G.T.TT.TTGTTTTTTTCTATTTCGCAATATTAATg    <  2:1556391/36‑1 (MQ=255)
                                  c.T.T.G.T.TT.TTGTTTTTTTCTATTTCGCAATATTAATg    >  1:3386959/1‑36 (MQ=255)
                                    t.t.g.t.tt.ttgtttTTTTCTATTTCGCAATATTAATGa   <  2:40208/36‑1 (MQ=255)
                                        |                                     
GAATTACTGGTGGGAATGTTTTTTTTA.GTTG.TC.T.T.G.T.TT.TTGTTTTTTTCTATTTCGCAATATTAATGAC  >  REL606/4462965‑4463034

Base quality scores:  ATCG  < 3 ≤  ATCG  < 5 ≤  ATCG  < 10 ≤  ATCG  < 22 ≤  ATCG  < 32 ≤  ATCG