Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6064,462,9970C→T54.0% 171.4214235yjgNconserved inner membrane protein
Reads supporting (aligned to +/- strand):  new base (1/101):  ref base (28/59):  total (29/185)

Only 100 of 220 total aligned reads displayed.

ATGGAATTACTGGTGGGAATGTTT.TTTTTA.G.TTGTCT.T.GTTT.TTGTTTT.TTTCTATTTCGCAATATTAATG  >  REL606/4462962‑4463032
                                      |                                       
  ggAATTACTGGTGGGAATGTTT.TTTTTA.G.TTGTCt.t                                      >  2:1705392/1‑36 (MQ=255)
    aaTTACTGGTGGGAATGTTT.TTTTTA.G.TTGTCt.t.gt                                   <  1:2088294/36‑1 (MQ=255)
    aaTTACTGGTGGGAATGTTT.TTTTTA.G.TTGTCt.t.gt                                   <  1:3261198/36‑1 (MQ=255)
      ttACTGGTGGGAATGTTT.TTTTTA.G.TTGTCt.t.gttt                                 <  2:1861329/36‑1 (MQ=255)
        aCTGGTGGGAATGTTT.TTTTTA.G.TTGTCt.t.gttt.tt                              >  1:3229533/1‑36 (MQ=255)
        aCTGGTGGGAATGTTT.TTTTTA.G.TTGTCt.c.tttt.tt                              >  1:1484458/1‑36 (MQ=37)
         cTGGTGGGAATGTTT.TTTTTA.G.TTGTCt.t.gttt.tag                             >  1:1564819/1‑34 (MQ=255)
          tggtggGAATGTTT.TTTTTA.G.TTGTTt.t.gttt.ttgt                            >  1:453135/1‑36 (MQ=255)
           ggtggGAATGTTT.TTGTTA.G.TTGTCt.t.gttt.ttgtt                           <  2:2616903/36‑1 (MQ=255)
                ttaTGTTT.TTTTT..T.TTCTCT.T.TTTT.TTTtttt.tttt                    <  1:3103366/34‑2 (MQ=0)
                gtttGTTT.TTTTTT.G.TTTTGT.T.TGTT.TTTtttt.ttt                     <  2:2831084/33‑1 (MQ=0)
                gtttGTTG.TTTTTT.T.TTGTTT.T.TTTT.TTTtttt.ttt                     <  2:3127329/33‑1 (MQ=0)
                gtgtGTTT.TTTTTT.G.TTTTTT.T.TTTT.TTTtttt.ttt                     <  2:3422158/33‑1 (MQ=0)
                gtgtGTTT.TTTTTC.G.TTCTCT.T.GTGT.TATtttc.ttt                     <  2:3617383/33‑1 (MQ=2)
                ggctGTTT.TTTTTT.T.TTATTT.T.TTTT.TTTtttt.ttt                     <  2:3356439/33‑1 (MQ=9)
                gactGTTT.TTTTTT.G.TTTTTT.T.TTTT.TTTtttt.ttt                     <  2:2851245/33‑1 (MQ=0)
                caaTGTTT.TTTTTT.A.TTTTTT.T.TTTT.TTTtttt.ttt                     <  1:3713303/35‑1 (MQ=9)
                 tttGTTT.TTTTTT.G.TGTTTT.T.TTTT.TTGtttt.tttt                    <  1:3651341/34‑2 (MQ=2)
                 tttGTTT.TTTTTC.T.TTGTTT.T.TTTT.TTCtttt.tttt                    <  1:2819985/34‑2 (MQ=9)
                 tttGTTT.TTTTTA.T.TTGTGT.T.GTTT.TTTtttt.tttt                    <  2:3662313/34‑2 (MQ=19)
                 tgtGTTT.TTGTTT.G.TTTTTT.T.TTTTGTTGtttt.ttt                     <  2:3039369/34‑1 (MQ=2)
                 tcggTTT.TTTTGT.G.TTTTTT.G.TTTT.TTGTTTT.TTTc                    <  1:3561262/33‑1 (MQ=2)
                 taTGTTT.TTTTTT.C.TT.TCT.T.TTTT.TTTtttt.ttttt                   <  1:2859545/35‑3 (MQ=0)
                 gttGTTT.TTGTTT.T.TTTTTT.T.GTTT.TTTtttt.tttt                    <  2:2844562/34‑2 (MQ=2)
                 ggtGTTT.TTTTTG.T.TTGTTT.T.TTTT.TTTtttt.tttt                    <  2:3644802/34‑2 (MQ=2)
                 cttGTTT.TTTTTT.T.TTCTTT.T.TTTT.TTTTTTT.TTTc                    <  2:2801217/34‑1 (MQ=2)
                 aaTGTTT.TTTTTA.G.TTGTCT.T.GTTT.TTGTTTT.TTTc                    <  2:68791/36‑1 (MQ=255)
                  tttttt.tttttT.T.TTGTCT.T.CTTT.TTTTTTT.TTTCt                   <  1:3035830/33‑1 (MQ=12)
                  ttGTTT.TTTTTT.T.TTTTCTCT.CTTT.TTCtttt.tttt                    <  1:3451909/35‑2 (MQ=2)
                  ttGTTT.TTTTTT.G.GTTTGG.T.GTTT.TTTtttt.ttttt                   <  2:3479566/35‑3 (MQ=0)
                  ttGTTT.CTTTTG.G.TGTTTT.T.TTTT.TTGtttt.ttttt                   <  2:3029344/35‑3 (MQ=2)
                  ttGTGT.TTTTTT.G.TTTTTT.T.CTTT.TTTtttt.ttttt                   <  2:3175584/35‑3 (MQ=2)
                  ttGTGT.TTTTTG.T.TTTTTT.T.TTTT.TTGtttt.ttttt                   <  2:3525010/35‑3 (MQ=2)
                  gtGTTT.TTTTTG.GTTTTTTT.T.GTTG.TTTtttt.tttt                    <  2:3413809/35‑2 (MQ=2)
                  gtGTTT.TTTTTATC.TTGTTT.T.TTTT.TTTtttt.cttt                    <  2:3000280/35‑2 (MQ=2)
                  gtGTTT.TGTTTA.T.TTGTTT.TCTTTT.TTGtttt.tttt                    <  2:3626551/35‑2 (MQ=2)
                  aTGTTTCTTTTTT.T.TTGATT.T.GTTT.TTTtttt.tttt                    <  2:2730392/36‑2 (MQ=9)
                  aTGTTT.TTTTTT.T.TTGTTG.T.TTTT.TTGtttt.ttttt                   <  2:3261942/36‑3 (MQ=9)
                  aTGTTT.TTTTTA.G.TTGTCT.T.GTTT.TTGTTTT.TTTCt                   <  1:2552385/36‑1 (MQ=255)
                   tcttt.tttttG.T.TTTTTT.T.GTTT.TTTTTTTATTTCt                   <  2:3497947/34‑1 (MQ=2)
                   tGTTT.TTTTTT.T.TTCTTT.T.GTTTATTGtttt.gtttg                   <  2:2871204/36‑3 (MQ=0)
                   tGTTT.TTTTTG.T.TTTTTT.T.GTTT.TTTTTTT.TTTCtt                  <  2:3561214/36‑2 (MQ=2)
                   tGTTT.TTTTTG.T.CTTTCT.T.TTTT.TTTtttt.tttttt                  <  2:3096926/36‑4 (MQ=2)
                   tGTTT.TTGTTT.C.TTGTTT.T.TTTT.TTTtttt.tttttt                  <  2:3493355/36‑4 (MQ=0)
                   tGTTT.GTTTTT.G.TTTTTT.T.GTTT.TTTtttt.ttttgt                  <  2:2920998/36‑4 (MQ=0)
                   tGTTG.TTTTTT.T.TTTTTT.T.GTTT.TTGtttt.tttttt                  <  2:2746914/36‑4 (MQ=0)
                    tttt.tttctT.T.TTTTCT.T.TTTT.TTCTTTT.TTTCttt                 <  2:3525606/35‑3 (MQ=2)
                    gTTT.TTTTTATC.TTTTTT.T.TTTT.TTTTTTT.TTTCtt                  <  1:3141462/36‑2 (MQ=9)
                    gTTT.TTTTTA.G.TTGTCT.T.GTTT.TTGTTTT.TTTCTAt                 <  2:2498419/36‑1 (MQ=255)
                    gTTT.TTTGTT.G.TTTTTT.T.GTGT.CCGTTTT.TTTCttt                 <  2:2781155/36‑3 (MQ=2)
                     ttt.tttttT.T.TTTTCT.T.TAAT.TTGTTTT.TTTCTTtt                <  2:3562546/36‑1 (MQ=2)
                     ttt.tttttC.G.TT.TTT.T.GTTC.TTTTTTT.TTTCTTttt               <  2:3580670/36‑1 (MQ=2)
                     ttt.tttttA...TGATCT.T.GTTT.GTTTTTT.TTTTTTttt               <  2:3590181/36‑1 (MQ=0)
                     ttt.gttttA.G.TT.TGT.T.GTTT.TTTTTTT.TTTTTTttt               <  2:3705812/36‑1 (MQ=2)
                     tgt.tttttT.T.TTGTCT.T..TTT.TT.TTTT.TTTCTTTTTc              <  2:3010261/34‑1 (MQ=0)
                     ctt.tttttT.T.TTGT.T.T.ATTT.TTGTTTT.TTTTTTttt               <  2:2738074/35‑1 (MQ=0)
                      tt.tttttT.T.TTG.CT.T.TTTT.TTTTTTT.TTTCTAtttt              <  2:3233796/36‑2 (MQ=2)
                      tt.tttttT.G.TTTTGT.T.TGTT.TTGTTTT.TTTTTTttt               <  2:3268381/36‑1 (MQ=0)
                      tt.tttttG.T.TTATTT.T.GTTT.TTGTTTT.TTTTTTttt               <  2:2730322/36‑1 (MQ=9)
                      tt.tttttA.G.TTGTCT.T.GTTT.TTGTTTT.TTTTTAttt               >  2:3395849/1‑36 (MQ=255)
                      tt.tttttA.G.TTGTCT.T.GTTT.TTGTTTT.TTTCTAttt               >  2:840184/1‑36 (MQ=255)
                      gt.tttttT.G.TGCTTT.T.GTTT.TTTTTTT.TTTTTTttt               <  2:2709220/35‑1 (MQ=12)
                      gt.tttctA.G.TTG..T.T.GTTT.TTGTTTT.TTTTTTttttt             <  2:3185326/35‑3 (MQ=2)
                      ct.tttttT.T.TTTTTT.T.GTTT.TTTTTTT.GTTCTTttt               <  2:3375967/35‑1 (MQ=2)
                       ttgttttT.G.TTCTTT.T.TTTT.TTGTTTT.TTTTTTttt               <  1:3368036/33‑1 (MQ=0)
                       t.tttttT.G.TCTTTT.T.GTTG.TTTTTTT.TTTTTAtttt              <  2:3442224/36‑2 (MQ=2)
                       t.tttttT.C.TTGTCT.T..TTT.TTGTTTT.TTTTTTttttt             <  2:3059264/36‑3 (MQ=0)
                       t.tttttG.T.TTGTGT.T.GTTT.TTTTTTT.TTTTTGtttt              <  2:3156306/36‑2 (MQ=9)
                       t.tttttG.G.TTTTTT.T.GTTT.TTCTTTT.TTTTTTtttt              <  2:3290965/36‑2 (MQ=2)
                       t.ttttcT.G.TTGTTT.T.GTTT.TTTTGTT.TTTTTTtttt              <  2:2841173/36‑2 (MQ=2)
                       t.ttttcT.G.TAGTTT.T.GTTT.TTTTTTT.TTTTTTtttt              <  2:3544286/36‑2 (MQ=0)
                       t.tttatA.T.TTTTTT.T.GTTT.TTTGTTT.TTTTTAtttt              <  2:3194733/36‑2 (MQ=0)
                        ttgtttA.G..TGTGT.T.GTTTGTTGTTTT.TTTTTTTTTc              <  2:3752070/33‑1 (MQ=0)
                        gtttttT.G.TTTTGT.T.GTTT.TTTTTTT.TTTTTTtttt              <  2:3239265/35‑2 (MQ=2)
                         tttttT.T.TTGTTT.T.GTTT.GTTTTTT.TTTCTTttttt             <  2:3717108/36‑3 (MQ=0)
                         tttttT.G.TTTTCT.T..TTT.TGTTTTT.TTTTTAtttttt            <  2:3635151/36‑4 (MQ=12)
                         tttttT.G.TTGTTT.G.TTTT.TTGTGTT.TTTGTTttttt             <  2:2960704/36‑3 (MQ=2)
                         tttttT.C.TTGTCT.T.TTTG.TTTTTTT.TTTTTTttttt             <  2:2940924/36‑3 (MQ=9)
                         tttttG.T.TTGTTT.T.GTGT.TTTTTTT.TTTTTTttttt             <  2:3146543/36‑3 (MQ=0)
                         tttttG.G.TTGTTT.T.TGTT.TTCTTTT.TTCCTTttttt             <  2:2950112/36‑3 (MQ=0)
                         tttttG.G.TTGTTT.T.ATTT.TTATTTG.TTTTTTttttt             <  2:3117471/36‑3 (MQ=2)
                         tttttG.C.TTGTTT.T.TTTG.TTGTTTT.TTTCTTttgtg             <  2:3328790/36‑4 (MQ=0)
                         tttttA.T.TTGTTG.T.TTTT.TTGTTTT.TTTTTTttttt             <  2:3300394/36‑3 (MQ=2)
                         tttttA.G.TTGTCT.T.GTTT.TTGTTTT.TTTCTATTTct             >  1:2475464/1‑35 (MQ=255)
                         tttttA.G.TTGTCT.T.GTTT.TTGTTTT.TTTCTATTTct             >  2:3310157/1‑35 (MQ=255)
                          ttttT.G.TTTCCT.T.TTTT.TTGTTTT.TTTTTTtttttt            <  2:3580883/36‑4 (MQ=2)
                           tttA.G.TTGTCT.T.GTTT.TTGTTTT.TTTCTATTTCGCa           >  2:2398218/1‑36 (MQ=255)
                            ttA.G.TTGTCT.T.GTTT.TTGTTTT.TTTCTATTTCTCaa          >  2:3645837/1‑36 (MQ=255)
                             tA.G.TTGTCT.T.GTTT.TTGTTTT.TTTCTATTTCGCAat         <  2:360651/36‑1 (MQ=255)
                             tA.G.TTGTCT.T.GTTT.TTGTTTT.TTTCTATTTCGCAat         >  2:1133594/1‑36 (MQ=255)
                              a.G.TTGTCT.T.GTTT.TTGTTTT.TTTCTATTTCTCAata        >  2:3439238/1‑36 (MQ=255)
                                   tGTCT.T.GTTT.TTGTTTT.TTTCTATTTCGCAATATTa     <  1:610406/36‑1 (MQ=255)
                                   tGTCT.T.GTTT.TTGTTTT.TTTCTATTTCGCAATATTa     >  1:347989/1‑36 (MQ=255)
                                   tGTCT.T.GTTT.TTGTTTT.TTTCTATTTCGCAATATTa     <  2:3101595/36‑1 (MQ=255)
                                     tCT.T.GTTT.TTGTTTT.TTTCTATTTCGCAATATTAAt   <  1:956878/36‑1 (MQ=255)
                                     tCT.T.GTTT.TTGTTTT.TTTCTATTTCGCAATATTAAt   >  1:3607742/1‑36 (MQ=255)
                                     tCT.T.GTTT.TTGTTTT.TTTCTATTTCGCAATATTAAt   >  2:55150/1‑36 (MQ=255)
                                      cT.T.GTTT.TTGTTTT.TTTCTATTTCGCAATATTAATg  <  2:1556391/36‑1 (MQ=255)
                                      cT.T.GTTT.TTGTTTT.TTTCTATTTCGCAATATTAATg  >  1:3386959/1‑36 (MQ=255)
                                      cT.T.GTTT.TTGTTTT.TTTCTATTTCGCAATATTAATg  >  2:926368/1‑36 (MQ=255)
                                      |                                       
ATGGAATTACTGGTGGGAATGTTT.TTTTTA.G.TTGTCT.T.GTTT.TTGTTTT.TTTCTATTTCGCAATATTAATG  >  REL606/4462962‑4463032

Base quality scores:  ATCG  < 3 ≤  ATCG  < 5 ≤  ATCG  < 11 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG