Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6063,357,0190G→T65.7% 92.2154intergenic (‑352/‑122)rrsD/yrdA16S ribosomal RNA/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/91):  ref base (13/35):  total (15/139)

Only 100 of 166 total aligned reads displayed.

CACAAGTATTTTTTTGATC.TT.T.TT.T.T.C.TGT.TTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAA  >  REL606/3356984‑3357053
                                           |                                  
cacaAGTATTTTTTTGATC.TT.T.TT.T.T.C.TGT.ttgttg                                    <  2:1861217/36‑1 (MQ=255)
  caAGTATTTTTTTGATC.TT.T.TT.T.T.C.TGT.TTgttgtt                                  >  2:150308/1‑36 (MQ=255)
   ttttATCTTTTTTTTC.TT.T.TT.T.T.C.T.T.TTTTTGtttt                                <  2:3432939/33‑1 (MQ=2)
   ttgTATTTTTTTCTTT.TT.T.TT.T.T.T.TTT.TTCTTTttt                                 <  2:3166042/34‑1 (MQ=2)
   taGTTTTTTTTTG.TT.TT.T.TT.T.T.C.TTT.TTTTTTtttt                                <  2:3725943/35‑1 (MQ=2)
   gtttATTTTTTTTATT.TT.T.TT.C.T.T.TTT.TTGTTTttt                                 <  2:3488730/33‑1 (MQ=0)
   ctttATTTTTTTGTTT.TT.T.TT.T.T.T.TCT.TTTTTTttt                                 <  2:3309762/33‑1 (MQ=0)
   caGTATTTTTCTGTTT.TT.T.TT.T.T.T.TTT.TTTTTTttt                                 <  1:3579472/35‑1 (MQ=2)
   atttATTTTTCTTTTC.TT.T.TT.T.T.C.TTT.TTTTTTttt                                 <  2:3068967/33‑1 (MQ=2)
   aaGTATTTTTTTGATC.TT.T.TT.T.T.C.TGT.TTGTTGttt                                 >  2:3378355/1‑36 (MQ=255)
   aaGTATTTTTTTGATC.TT.T.TT.T.T.C.TGT.TTGTTGttt                                 >  2:1905400/1‑36 (MQ=255)
    tttATTCTTTAGTTC.TT.T.TT.T.T.A.TTT.TTTTTTtttt                                <  1:2780546/34‑1 (MQ=2)
    tgTATTTTTTT.AT..TT.T.TC.T.T.T.TTT.TTGTTTtttttt                              <  1:3006721/35‑1 (MQ=9)
    tgTATTTTGTTTTTC.TT.T.TT.T.T...TGT.TTTTTTttttt                               <  2:2831109/35‑1 (MQ=0)
    gttATTTTTTTGTTT.AT.T.TT.T.T.T.TTT.TTTTTTtttt                                <  2:2960734/34‑1 (MQ=9)
     ttttttttttTATA.TT.T.TT.T.T.C.TAT.TTGTTTttttt                               <  2:3599358/33‑1 (MQ=12)
     ttttttttttGTTA.TT.T.TT.T.T.T.TTT.CTGTTTttttt                               <  2:2901269/33‑1 (MQ=2)
     ttttttttttCTTC.TT.T.TT.G.T.T.TTT.TTGTTTttttt                               <  2:2940322/33‑1 (MQ=2)
     ttgtttttttTTTC..T.T.TT.T.T.C.TGT.TTCTTTtttttt                              <  2:3525773/33‑1 (MQ=2)
     ttgtttttttTATC.TT.T.TT.T.T.C.TTT.TTTTTTttttt                               <  2:3460934/33‑1 (MQ=12)
     ttctttttttG.TT.TT.T.TT.G.T.C.TTT.TTTTTTtttttt                              <  2:3243433/33‑1 (MQ=0)
     tcgtttttttG.TT.TT.T.TT.T.T.C.TTT.TTTTTTtttttt                              <  2:3049520/33‑1 (MQ=2)
     tattttttttGGTT.TT.T.TT.T.T.C.TGT.TTTTTCctttt                               >  2:2443900/4‑36 (MQ=0)
     gtgtttttttG.TT.TT.T.TT.T.T.T.TTT.TTCTTTtttttt                              <  2:3689767/33‑1 (MQ=0)
     gTATTTTTTTCATC.TT.T.TT.T.T.C.TGT.TTGTTGttttt                               >  2:2030423/1‑36 (MQ=255)
     ctttttttttTCTC.TT.T.TT.T.T...TCT.TTTTTTtttttt                              <  2:3507277/33‑1 (MQ=2)
     ctttttgtttGA.C.TT.C.TT.T.T.C.TTT.TTTTTTtttttt                              <  2:3347774/33‑1 (MQ=2)
     ctgtttttttGATT.TT.T.TT.T.T.T.TTT.TTTTTTttttt                               <  1:2760623/33‑1 (MQ=9)
     atttttttttGTTT.TT.T.TA.T.T.T.TGT.TTTTTTttttt                               <  2:3699113/33‑1 (MQ=9)
      tttttttttGTTC.TT.T.TT.T.T.T.TTT.TTATTTcttttt                              <  2:3233956/34‑1 (MQ=2)
      tttttttttGTTC.TT.T.TA.T.T...TTT.TTGTTTttttttt                             <  2:3571376/34‑2 (MQ=0)
      tttttttttGTTC.TT.A.TT.G.T.C.TTG.TTTTTTtttttt                              <  2:3059305/34‑1 (MQ=2)
      ttttttttaGTTC.TT.T.TT.T.T.T.TTT.GTTTTTtttttt                              <  1:68746/34‑1 (MQ=0)
      tttttatttTATT.TT.T.TT.T.T.C.TTT.TTTTTGtttttt                              <  2:3291089/34‑1 (MQ=2)
      tgtttttttGTTT.TC.T.TT.T.T.T.TTT.TTTTTCtttttt                              <  2:3137028/34‑1 (MQ=9)
      tgtttttttGTTG.TT.T.TT.T.T.G.TTT.TTCTGTtttttt                              <  2:2789881/34‑1 (MQ=2)
      tgtttttttG.TT.TT.T.TG.T.T.T.TGT.TTTTTTttttttt                             <  1:3349340/34‑2 (MQ=12)
      tgtttttttCTTG.TG.T.TT.T.T.T.TGT.TTTTTGttgttt                              <  2:3337638/34‑1 (MQ=2)
      tgttttcttTATCTTT.T.TT.T.T.C.TAT.TTCTTTttttt                               <  2:3681032/34‑1 (MQ=0)
      tcttttgttTATC.TT.T.TT.T.T.T.TTT.TTTTTTtttttt                              <  1:3451911/34‑1 (MQ=2)
      tATTTTATTTTTC.TT.T.TT.T.T.T.TTT.TTTTTCtttttt                              <  1:3561323/36‑1 (MQ=0)
      gttttttttGTTT.TT.T.GT.T.T.T.GTT.TTTTTGtttttt                              <  2:3553093/34‑1 (MQ=2)
      gtttttcttGATC.TT.T.TT.T.T.T.TTT.TTTTTTtttttt                              <  1:3730625/34‑1 (MQ=9)
      gtgttttttTTTC.TT.T.TT.T.T.T.TGT.TTTTTTtttttt                              <  2:3507140/33‑1 (MQ=0)
      gtgttttttGTTT.TT.T.TT.T.T.C.TTG.TTTTTTtttttt                              <  1:2720006/33‑1 (MQ=0)
      gaTTTTGTTTTTC.TT.T.TT.T.T.T.TTT.TTTTTTtttttt                              <  2:2778035/35‑1 (MQ=0)
      ctttttcttGTTG.TT.T.TT.T.T.T.TTT.TTCTTTtttttt                              <  2:2870397/34‑1 (MQ=2)
      cttttatttTATT.TT.T.TT.T.T.T.TGT.TTTGTTtttttt                              <  2:3224270/34‑1 (MQ=2)
      cggtttttcTATT.TT.T.TT.T.G.T.TTT.TTGTTGtttttt                              <  2:3617270/33‑1 (MQ=2)
      cctttttttCTTC.TT.T.TT.T.T.T.TTT.TTTTTTtttttt                              <  2:3328767/34‑1 (MQ=2)
      caTTTTTTTCTTC.TT.TCTT.T.T.T.TTT.TTTTTGttttt                               <  2:3498076/35‑1 (MQ=2)
      attttttttTCTA.TT.T.TT.C.T.T.TTT.TTTTTGtttttt                              <  2:3717015/34‑1 (MQ=0)
       ttttttttTTTT.TT.T.TT.T.T.G.TTG.TTGTTTttttttg                             <  2:3078788/35‑2 (MQ=2)
       ttttttttTATC.TT.T.TT.TCT.T.TTT.TTTTTCtttttt                              <  2:2930924/35‑1 (MQ=2)
       ttttttttGATG.TT.T.TTCT.T.T.TCT.TTTTTTtttttt                              <  1:3405735/35‑1 (MQ=0)
       ttttttttCTTT.TT.T.TT.T.T.T.TGTGTTTTTGtttttt                              <  2:3127379/35‑1 (MQ=2)
       ttttttgtGTTT.TG.T.TT.T.T.T.TGT.TTTTTTttttttt                             <  2:3214593/35‑2 (MQ=2)
       gtttttttCTTT.TT.T.TT.T.T.C.TTT.TTTCTCttttttt                             <  2:3272078/35‑2 (MQ=0)
       ctttttttG.TT.TT.T.TT.T.T.C.TTT.TTTTTTTTTTTTct                            <  2:3525816/35‑2 (MQ=2)
       ctttatttGTTT.TT.T.TT.T.G.T.TGT.TTTTTTttttttt                             <  2:3543707/35‑2 (MQ=2)
       ccttttttGTTT.TT.T.TT.T.T.T.TGT.TTTTTTttttttt                             <  1:3668973/34‑2 (MQ=2)
       aTTTTTTTTTTC.TT.T.TT.T.T.T.TCT.TTCTTTTTTTCTc                             >  2:639598/1‑36 (MQ=2)
       aTTTTTTTGATT.GT.T.TT.T.T.T.TTT.TCTTTCttttttt                             <  2:3414295/36‑2 (MQ=2)
        tttttttTTTT.TT.T.TT.C.T.C.CGT.TTTTTTtttttttt                            <  2:2871380/36‑3 (MQ=2)
        tttttttTTTT.TT.G.TT.T.T.CTTTT.TTGTTGttttttt                             <  2:3039719/36‑2 (MQ=2)
        tttttttTGTT.TT.T.TT.T.T.C.TTT.TTGTTCTTTTTTct                            <  2:3338294/36‑2 (MQ=12)
        tttttttTGTT.TT.T.TT.G.T.T.TTT.TTGTTGttgtttgt                            <  2:3020148/36‑3 (MQ=2)
        tttttttTATT.TT.G.TT.T.T.C.TGT.TTTTTTtttttttt                            <  2:3562518/36‑3 (MQ=2)
        tttttttGTTT.TT.G.TT.G.T.T.TTT.TTTTTGtttttttt                            <  2:2750882/36‑3 (MQ=2)
        tttttttGTTC.TT.T.TT.T.T.T.TTTCTTTTTGttttttt                             <  1:2849565/36‑2 (MQ=2)
        tttttttGATT.TT.T.TT.TGT.C.TTT.TTTTTTttttttt                             <  1:3615383/36‑2 (MQ=9)
        tttttttGATC.TT.T.TT.T.T.C.TGT.TTGTTGTTTTTTCa                            <  2:982294/36‑1 (MQ=255)
        tttttttCTTC.TT.T.TT.T.T...TCT.TTATTTttttttttt                           <  1:2800152/36‑4 (MQ=2)
        tttttttAATT.TT.T.TT.T.TAG.TTT.TTGTTTttttttt                             <  2:3743376/36‑2 (MQ=2)
        tttttgtGTTT.TT.T.TT.T.T.C.TTT.CTTTTTtttttttt                            <  2:2920789/36‑3 (MQ=2)
        tttgtttTATC.TT.T.TT.T.T.T.TCT.TTTTTTtttttttt                            <  2:3010165/36‑3 (MQ=0)
        gttttttGTTC.TT.T.TT.TCT.T.TGT.TTTTTTttttttt                             <  2:3020198/35‑2 (MQ=2)
        gtttgttGCTT.TT.G.TT.T.T.T.TCT.TTGTTGtttttttt                            <  2:3423338/35‑3 (MQ=0)
        cttttttCGTC.TT.T.TT.T.T.T.TTT.TTTTTTtttttttt                            <  2:3690010/35‑3 (MQ=0)
        attttttTCTT.TT.T.TT.T.T.T.TGT.TTTTTCtttttttt                            <  2:3010287/35‑3 (MQ=9)
         ttttttTCTT.TT.T.GT.T.T.C.TTT.TTGTTTttttttttt                           <  2:3127276/36‑4 (MQ=0)
         ttttttGTTT.TTGT.TT.T.T.C.T.T.TTGTTTttttttttt                           <  2:3732058/36‑4 (MQ=2)
         ttttttGTTT.TT.T.TT.T.T.T.CTT.TTTTTGttttttttc                           <  2:3507124/36‑4 (MQ=9)
         tttcttGTTG.TT.T.TT.T.T.T.TGT.TTTTTTttttttttt                           <  2:3281766/36‑4 (MQ=2)
          tttttGTTC.TT.T.TT.T.TGG.TGT.TTTTTCttttttttt                           <  2:3608448/36‑4 (MQ=2)
          tttttGATC.TT.T.TT.T.T.C.TGT.TTGTTGTTTTTTCatc                          >  2:2970731/1‑34 (MQ=255)
          tttttGATC.TT.T.TT.T.T.C.TGT.TTGTTGTTTTTTCAcc                          >  2:2961690/1‑36 (MQ=255)
          tttttGATC.TT.T.TT.T.T.C.TGT.TTGTTGTTTTTTCAcc                          >  2:842307/1‑36 (MQ=255)
              tGATC.TT.T.TT.T.T.C.TGT.TTGTTGTTTTTTCACCCttt                      <  2:1467007/36‑1 (MQ=255)
                     t.t.tt.t.t.C.TGT.TTGTTGTTTTTTCACCCTTTTtgttg                >  1:294242/1‑36 (MQ=255)
                            t.t.C.TGT.TTGTTGTTTTTTCACCCTTTTTGCTGCAAt            <  1:2541083/36‑1 (MQ=255)
                            t.t.C.TGT.TTGTTGTTTTTTCACCCTTTTTGCTGCAAt            >  2:372498/1‑36 (MQ=255)
                                   gt.ttgttGTTTTTTCACCCTTTTTGCTGCAATCGca        <  1:1409564/36‑1 (MQ=255)
                                    t.ttgttGTTTTTTCACCCTTTTTGCTGCAATCGcac       >  2:2420502/1‑36 (MQ=255)
                                    t.ttgttGTTTTTTCACCCTTTTTGCTGCAATCGcac       <  1:2328824/36‑1 (MQ=255)
                                    t.ttgttGTTTTTTCACCCTTTTTGCTGCAATCGcac       <  1:2020032/36‑1 (MQ=255)
                                    t.ttgttGTTTTTTCACCCTTTTTGCTGCAATCGcac       <  1:1166855/36‑1 (MQ=255)
                                        gttgttTTTTCACCCTTTTTGCTGCAATCGcacaca    <  1:3208967/36‑1 (MQ=255)
                                        gttgttTTTTCACCCTTTTTGCTGCAATCGcacaca    <  1:975752/36‑1 (MQ=255)
                                        gttgttTTTTCACCCTTTTTGCTGCAATCGcacaca    <  1:2030589/36‑1 (MQ=255)
                                           |                                  
CACAAGTATTTTTTTGATC.TT.T.TT.T.T.C.TGT.TTGTTGTTTTTTCACCCTTTTTGCTGCAATCGCACACAAA  >  REL606/3356984‑3357053

Base quality scores:  ATCG  < 3 ≤  ATCG  < 5 ≤  ATCG  < 8 ≤  ATCG  < 18 ≤  ATCG  < 32 ≤  ATCG