Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6064,462,9910A→T61.1% 119.0207229yjgNconserved inner membrane protein
Reads supporting (aligned to +/- strand):  new base (1/101):  ref base (28/37):  total (29/178)

Only 100 of 210 total aligned reads displayed.

TTCTTATGGAATTACTGGTGGGAATGTTTTTTTTA.GTT.GTC.T.T.GT.TT.TTGTTTT.TTTCTATTTCGCAATA  >  REL606/4462957‑4463026
                                  |                                           
ttCTTATGGAATTACTGGTGGGAATGTTTTTTTTat                                            >  2:3152760/1‑35 (MQ=255)
   ttATGGAATTACTGGTGGGAATGTTTTTTTTA.GTT.g                                       <  1:3479190/36‑1 (MQ=255)
       ggAATTACTGGTGGGAATGTTTTTTTTA.GTT.GTC.t.t                                 >  2:1705392/1‑36 (MQ=255)
           ttACTGGTGGGAATGTTTTTTTTA.GTT.GTC.t.t.gt.tt                           <  2:3141854/36‑1 (MQ=255)
           ttACTGGTGGGAATGTTTTTTTTA.GTT.GTC.t.t.gt.tt                           <  2:1861329/36‑1 (MQ=255)
           ttACTGGCGGGAATGTTTTTTTTA.GTT.GTC.t.t.tt.tt                           >  2:918333/1‑36 (MQ=37)
             aCTGGTGGGAATGTTTTTTTTA.GTT.GTC.t.c.tt.tt.tt                        >  1:1484458/1‑36 (MQ=37)
               tggtggGAATGTTTTTTTTA.GTT.GTT.t.t.gt.tt.ttgt                      >  1:453135/1‑36 (MQ=255)
                ggtggGAATGTTTTTGTTA.GTT.GTC.t.t.gt.tt.ttgtt                     <  2:2616903/36‑1 (MQ=255)
                 gtggGAATGTTTTTTTTA.GTT.GTC.t.t.gt.tt.ttgttt                    >  2:1585412/1‑36 (MQ=255)
                    cttaTGTTTTTTTT..TTT.TTC.T.T.GT.TT.TTTtttt.ttt               <  2:3078838/33‑1 (MQ=12)
                     ttctGTTTTTTTTT.GTT.TTT.T.T.TT.CT.TTTtttt.ttt               <  1:3265404/33‑1 (MQ=0)
                     tgttGTTTTTTTTT.GTT.TTT.TGT.GT.TT.TTTtttt.tt                <  2:3028801/33‑1 (MQ=2)
                     tgttGTTTTTTTTT.GTG.GTT.T.T.TT.TT.TTTcttt.ttt               <  1:2908813/33‑1 (MQ=0)
                     tggtGTTTTTTTTT.CTT.TTT.T.T.TT.TT.TTTtttt.ttt               <  1:3713311/33‑1 (MQ=2)
                     tcttGTTTTTTTCT.GTT.TTT.T.T.TT.TT.TTTtttt.ttt               <  2:2850979/33‑1 (MQ=2)
                     gtttGTTTTTTTTT.GTT.TTG.T.T.TG.TT.TTTtttt.ttt               <  2:2831084/33‑1 (MQ=0)
                     gtttGTTTTTTTTG.TTT.GTT.T.T.TT.TT.TTTtttt.ttt               <  2:3338190/33‑1 (MQ=2)
                     gtttGTTGTTTTTT.TTT.GTT.T.T.TT.TT.TTTtttt.ttt               <  2:3127329/33‑1 (MQ=0)
                     gtgtGTTTTTTTTC.TTT.TTT.T.T.CT.TT.TTTtttt.ttt               <  1:3424159/33‑1 (MQ=0)
                     atgtGTGTTTTTTT.TTT.TTT.T.T.GT.TT.TTTtttt.ttt               <  1:3668999/33‑1 (MQ=2)
                      tttGTTTTTTTTT.TTT.CTC.T.T..T.TT.TTCTTTT.CTTCt             <  2:3690020/34‑1 (MQ=0)
                      tttGTTTTTTTTC.TTT.GTT.T.T.TT.TT.TTCtttt.tttt              <  1:2819985/34‑2 (MQ=9)
                      tttGTTTTTTATC.CTT.GTT.T.T.TT.TT.TTTtttt.tttt              <  2:3290985/34‑2 (MQ=0)
                      tttGTTTTTGTTT.TTT.TTG.T.T.GT.TT.TTTtttt.tttt              <  2:3404819/34‑2 (MQ=12)
                      tgtGTTTTTATCT.GTT.GTT.T.TCGT.TT.TTGtttt.ttt               <  2:3644839/34‑1 (MQ=2)
                      tctGTTTTTTTTT.CTT.GTT.T.T.TT.TT.CTTtttt.tttt              <  2:2761098/34‑2 (MQ=0)
                      tctGTTTTTTTTATGTT.TTT.T.T.TT.TT.TTTtttt.ttt               <  1:3237185/34‑1 (MQ=2)
                      tctGTTTTTTCTT.TTT.TTT.T.T.TT.TT.TTGTTTT.TTTc              <  1:2720065/34‑1 (MQ=0)
                      tcggTTTTTTTGT.GTT.TTT.T.G.TT.TT.TTGTTTT.TTTc              <  1:3561262/33‑1 (MQ=2)
                      taTGTTTTTTTT..GTT.TTT.T.T.GT.TT.CTTtttt.ttttt             <  2:3642793/35‑3 (MQ=2)
                      gttGTTTTTGTTT.TTT.TTT.T.T.GT.TT.TTTtttt.tttt              <  2:2844562/34‑2 (MQ=2)
                      gttGTTTTTATTA.TTT.TTC.T.T.GT.TT.TTTtttc.tttt              <  2:2960782/34‑2 (MQ=9)
                      gaTGTTTTTTTTA.GTT.GTC.T.T.GT.TT.TTGTTTT.TTTc              <  1:834183/35‑1 (MQ=255)
                      cttGTTTTTTTTT.TTT.CTT.T.T.TT.TT.TTTTTTT.TTTc              <  2:2801217/34‑1 (MQ=2)
                      cttGTTTTTTTTT.GTT.TTT.T.T.CT.TT.TTTtttt.tttt              <  2:3000471/34‑2 (MQ=2)
                      cgtGTGTTTTGTT.GTT.TTT.T.T.TT.TT.TTGtttt.tttt              <  2:3304987/34‑2 (MQ=0)
                      aaTGTTTTTTTTA.GTT.GTC.T.T.GT.TT.TTGtttt.tttt              >  1:2248449/1‑35 (MQ=255)
                      aaTGTTTTTTTTA.GTT.GTC.T.T.GT.TT.TTGTTTT.TTTc              <  2:68791/36‑1 (MQ=255)
                       ttGTTTTTTTTT.GTT.TGT.T.T.TT.TT.TTTtttt.tttgt             <  2:3525672/35‑3 (MQ=0)
                       ttGTTTTTTTTT.GGT.TTG.G.T.GT.TT.TTTtttt.ttttt             <  2:3479566/35‑3 (MQ=0)
                       ttGTGTTTTTTT.GTT.TTT.T.T.CT.TT.TTTtttt.ttttt             <  2:3175584/35‑3 (MQ=2)
                       ttGTGTTTTTTG.TTT.TTT.T.T.TT.TT.TTGtttt.ttttt             <  2:3525010/35‑3 (MQ=2)
                       gtGTTTTTTTTA.TTT.TTT.T.T.TC.TT.TTTtttt.ttttt             <  2:2730439/35‑3 (MQ=2)
                       ctGTTGTTTTTC.GTT.TTT.T.T.GT.GT.TTTtttt.ttttt             <  1:3074505/35‑3 (MQ=0)
                       aTGTTTTTTTTT.TTT.GTT.G.T.TT.TT.TTGtttt.ttttt             <  2:3261942/36‑3 (MQ=9)
                       aTGTTTTTTTTA.GTT.GTC.T.T.GT.TT.TTGTTTT.TTTCt             <  2:3421301/36‑1 (MQ=255)
                       aTGTTTTTTTTA.GTT.GTC.T.T.GT.TT.TTGTTTT.TTTCt             >  1:1883715/1‑36 (MQ=255)
                       aTGTTTTTTTTA.GTT.GTC.T.T.GT.TT.TTGTTTT.TTTCt             <  1:2552385/36‑1 (MQ=255)
                        tcttttttttG.TTT.TTT.T.T.GT.TT.TTTTTTTATTTCt             <  2:3497947/34‑1 (MQ=2)
                        tGTTTTTTTTT.TTT.TTC.T.T.TT.CT.TTCtttt.tttttt            <  1:3624370/36‑4 (MQ=2)
                        tGTTTTTTTTT.TTT.CTT.T.T.GT.TTATTGtttt.gtttg             <  2:2871204/36‑3 (MQ=0)
                        tGTTTTTTTTT.GTT..TT.T.T.GT.TT.TTTTTTT.TTTCttt           <  2:3048620/36‑3 (MQ=0)
                        tGTTTTTTTTG.TTT.TTT.T.T.GT.TT.TTTTTTT.TTTCtt            <  2:3561214/36‑2 (MQ=2)
                        tGTTTTTTTCT.TTT.GTC.T.T.TT.GT.TTTtttt.ttttgt            <  1:3170156/36‑4 (MQ=9)
                        tGTTTTTGTTT.CTT.GTT.T.T.TT.TT.TTTtttt.tttttt            <  2:3493355/36‑4 (MQ=0)
                        tGTTTGTTTTT.GTT.TTT.T.T.GT.TT.TTTtttt.ttttgt            <  2:2920998/36‑4 (MQ=0)
                        tGTTGTTTTTC.TTT.TTC.T.T.TT.TT.TTTtttt.ttttct            <  2:3098276/36‑4 (MQ=0)
                         tttttttttT.GTT.GT..T.T..T.TT.GTGTTTT.TTTTTTttt         <  2:2959544/35‑1 (MQ=2)
                         tttttttttA.GTT.GTC.T.T.GT.TT.TTGTTTT.TTTCttt           >  2:3728621/2‑34 (MQ=255)
                         tttttttctC.GTT..TC.T.T.CT.TT.TT.TTTT.TTTCTTttt         <  1:3479325/35‑1 (MQ=0)
                         gTTTTTTTTA.GTT.GTC.T.T.GT.TT.TTGTTTT.TTTCttt           >  1:311559/1‑34 (MQ=255)
                         gTTTTTTTTA.GTT.GTC.T.T.GT.TT.TTGTTTT.TTTCTAt           <  2:2498419/36‑1 (MQ=255)
                         gTTTTTTGTT.GTT.TTT.T.T.GT.GT.CCGTTTT.TTTCttt           <  2:2781155/36‑3 (MQ=2)
                         gTTCTTTTTG.GTTCGTG.T.T.TT.TT.TTGtttt.tttttt            <  2:3451252/36‑4 (MQ=0)
                          ttttttttT.TTT.TTC.T.T.TA.AT.TTGTTTT.TTTCTTtt          <  2:3562546/36‑1 (MQ=2)
                          ttttttttG.TTT.GT..T.T.GT.TT.TT.TTTT.TTTCTTtttt        <  2:2730337/36‑2 (MQ=0)
                          ttttttttC.GTT..TT.T.T.GT.TC.TTTTTTT.TTTCTTttt         <  2:3580670/36‑1 (MQ=2)
                          gcttttttT.GTT..TT.T.T.GT.TT.TTTTTTT.TTTTTTttt         <  2:3328129/34‑1 (MQ=0)
                           tttttttT.TTT.G.C.T.T.TT.TT.TTTTTTT.TTTCTAtttt        <  2:3233796/36‑2 (MQ=2)
                           tttttttT.GTT.TTG.T.T.TG.TT.TTGTTTT.TTTTTTttt         <  2:3268381/36‑1 (MQ=0)
                           tttttttT.GTT..TT.T.T.GT.TT.TT.TTTT.TTTGTAttttt       <  2:2741429/36‑3 (MQ=9)
                           tttttttC.TTT.TTC.T.C.TT.TT.TTTTTTT.TTTCTTttt         <  2:3608443/36‑1 (MQ=0)
                           tggttttT.GTT.GTT.T.T.GT.TT.TTTTTTT.TTTTTTttt         <  2:3366436/33‑1 (MQ=0)
                           gttttttT.GTG.CTT.T.T.GT.TT.TTTTTTT.TTTTTTttt         <  2:2709220/35‑1 (MQ=12)
                           gttttttG.GTT.TTT.T.T.TT.TT.TTGTTTT.TTTTTTttt         <  2:3589004/35‑1 (MQ=2)
                           gttttctA.GTT.G...T.T.GT.TT.TTGTTTT.TTTTTTttttt       <  2:3185326/35‑3 (MQ=2)
                           cttttttT.GTT.TTT.T.T.TT.TT.TTGTTTT.TTT.TAtttt        <  2:3590183/35‑2 (MQ=0)
                            ttttttT.GTT.TTG.T.G.TT.TT.TTGTTTT.TTTTTTtttt        <  2:3442072/36‑2 (MQ=0)
                            ttttttG.TTT.GTG.T.T.GT.TT.TTTTTTT.TTTTTGtttt        <  2:3156306/36‑2 (MQ=9)
                            ttttttG.GTT.TTT.T.T.GT.TT.TTCTTTT.TTTTTTtttt        <  2:3290965/36‑2 (MQ=2)
                            ttttttA.TTT.TTA.T.T.GTGTT.TTTTTTT.TTTCTCttt         <  2:3376025/36‑1 (MQ=2)
                            tttttcT.GTT.TTT.T.T.GT.TT.TTTTTTC.TTTTTAttgt        <  2:3029695/36‑3 (MQ=0)
                            tttttcT.GTT.GTT.T.T.GT.TT.TTTTGTT.TTTTTTtttt        <  2:2841173/36‑2 (MQ=2)
                            tttttcT.GTA.GTT.T.T.GT.TT.TTTTTTT.TTTTTTtttt        <  2:3544286/36‑2 (MQ=0)
                            ttttatA.TTT.TTT.T.T.GT.TT.TTTGTTT.TTTTTAtttt        <  2:3194733/36‑2 (MQ=0)
                            ttgtttA.G.T.GTG.T.T.GT.TTGTTGTTTT.TTTTTTTTTc        <  2:3752070/33‑1 (MQ=0)
                            gtttttT.GTT.TTG.T.T.GT.TT.TTTTTTT.TTTTTTtttt        <  2:3239265/35‑2 (MQ=2)
                            gattttT.GTT.TTT.T.T.GT.TT.TTTTTTT.TTTTTTtttt        <  2:3717069/34‑2 (MQ=0)
                             tttttT.GTT.TTC.T.T..T.TT.TGTTTTT.TTTTTAtttttt      <  2:3635151/36‑4 (MQ=12)
                             tttttT.CTT.GTC.T.T.TT.TG.TTTTTTT.TTTTTTttttt       <  2:2940924/36‑3 (MQ=9)
                             tttttG.GTT.GTT.T.T.TG.TT.TTCTTTT.TTCCTTttttt       <  2:2950112/36‑3 (MQ=0)
                             tttttG.GTT.GTT.T.T.AT.TT.TTATTTG.TTTTTTttttt       <  2:3117471/36‑3 (MQ=2)
                             tttttG.GTT.GTT.G.T.TT.TT.GTGTTTT.TTTTTTttttt       <  2:3648000/36‑3 (MQ=0)
                             tttttA.GTT.GTC.T.T.GT.TT.TTGTTTT.TTTCTATTTct       >  1:2475464/1‑35 (MQ=255)
                             ttttgA.GTT.GTT.T.T.GC.TT.TTGTTTT.TTTTTTttttt       <  2:3617419/36‑3 (MQ=9)
                             cttttT.GTT.TTCTT.T.GT.TT.TTTTTTT.TTTTTTtttt        <  2:3423397/35‑2 (MQ=9)
                              ttttT.GTT.TCC.T.T.TT.TT.TTGTTTT.TTTTTTtttttt      <  2:3580883/36‑4 (MQ=2)
                               tttA.GTT.GTC.T.T.GT.TT.TTGTTTT.TTTCTATTTCGCa     >  2:2398218/1‑36 (MQ=255)
                                 tA.GTT.GTC.T.T.GT.TT.TTGTTTT.TTTCTATTTCGCAat   >  2:1133594/1‑36 (MQ=255)
                                  |                                           
TTCTTATGGAATTACTGGTGGGAATGTTTTTTTTA.GTT.GTC.T.T.GT.TT.TTGTTTT.TTTCTATTTCGCAATA  >  REL606/4462957‑4463026

Base quality scores:  ATCG  < 3 ≤  ATCG  < 5 ≤  ATCG  < 10 ≤  ATCG  < 21 ≤  ATCG  < 32 ≤  ATCG