Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6064,463,0060G→T51.7% 204.0198244yjgNconserved inner membrane protein
Reads supporting (aligned to +/- strand):  new base (0/93):  ref base (21/66):  total (21/177)

Only 100 of 218 total aligned reads displayed.

CTGGTGGGAATGTTTTTTTTA.G.TT.G.TCT.T.G.TTT.TTGTTTT.TTTCTATTTCGCAATATTAATGACAGTGTC  >  REL606/4462971‑4463040
                                           |                                   
cTGGTGGGAATGTTTTTTTTA.G.TT.G.TCt.t.g.ttt.ttg                                     <  2:3267943/36‑1 (MQ=255)
   gtggGAATGTTTTTTTTA.G.TT.G.TCt.t.g.ttt.ttgttt                                  >  2:1585412/1‑36 (MQ=255)
    tggGAATGTTTTTTTTA.G.TT.G.TCt.t.g.ttt.ttgttta                                 >  1:533572/1‑35 (MQ=255)
    tggGAATGTTTTTTTTA.G.TT.G.TCT.t.g.ttt.ttgtttt                                 >  2:47085/1‑36 (MQ=255)
      cttaTGTTTTTTTT..T.TT.T.TCT.T.G.TTT.TTTtttt.ttt                             <  2:3078838/33‑1 (MQ=12)
       ttctGTTTTTTTTT.G.TT.T.TTT.T.T.TCT.TTTtttt.ttt                             <  1:3265404/33‑1 (MQ=0)
       tgttGTTTTTTTTT.G.TT.T.TTTGT.G.TTT.TTTtttt.tt                              <  2:3028801/33‑1 (MQ=2)
       tgttGTTTTTTTTT.G.TG.G.TTT.T.T.TTT.TTTcttt.ttt                             <  1:2908813/33‑1 (MQ=0)
       tcttGTTTTTTTTC.T.AT.G.TTT.T.T.TTT.TTTtttt.ttt                             <  2:2841221/33‑1 (MQ=0)
       tcttGTTTTTTTCT.G.TT.T.TTT.T.T.TTT.TTTtttt.ttt                             <  2:2850979/33‑1 (MQ=2)
       gtttGTTTTTTTTG.T.TT.G.TTT.T.T.TTT.TTTtttt.ttt                             <  2:3338190/33‑1 (MQ=2)
       gtgtGTTTTTTTTT.G.TT.T.TTT.T.T.TTT.TTTtttt.ttt                             <  2:3422158/33‑1 (MQ=0)
       gtgtGTTTTTTTTC.G.TT.C.TCT.T.G.TGT.TATtttc.ttt                             <  2:3617383/33‑1 (MQ=2)
       caaTGTTTTTTTTT.A.TT.T.TTT.T.T.TTT.TTTtttt.ttt                             <  1:3713303/35‑1 (MQ=9)
        tttGTTTTTTTTT.G.TG.T.TTT.T.T.TTT.TTGtttt.tttt                            <  1:3651341/34‑2 (MQ=2)
        tttGTTTTTTTTA.T.TT.G.TGT.T.G.TTT.TTTtttt.tttt                            <  2:3662313/34‑2 (MQ=19)
        tttGTGTTTTTTT.T.TT.G.TTT.T.G.TTT.TTTtttt.tttt                            <  2:3107948/34‑2 (MQ=2)
        tgtGTTTTTTTTT.T.TT.T.TTT.T.C.TTT.TTCtttt.tttt                            <  2:2940633/34‑2 (MQ=2)
        tctGTTTTTTCTT.T.TT.T.TTT.T.T.TTT.TTGTTTT.TTTc                            <  1:2720065/34‑1 (MQ=0)
        tcggTTTTTTTGT.G.TT.T.TTT.G.T.TTT.TTGTTTT.TTTc                            <  1:3561262/33‑1 (MQ=2)
        taTGTTTTTTTTT.G.TC.T.TTT.T.T.TAT.TTTtttt.tttt                            <  1:3151048/35‑2 (MQ=0)
        taTGTTTTTTTTT.C.TT...TCT.T.T.TTT.TTTtttt.ttttt                           <  1:2859545/35‑3 (MQ=0)
        gttGTTTTTATTA.T.TT.T.TCT.T.G.TTT.TTTtttc.tttt                            <  2:2960782/34‑2 (MQ=9)
        ggtGTTTTTTTTG.T.TT.G.TTT.T.T.TTT.TTTtttt.tttt                            <  2:3644802/34‑2 (MQ=2)
        gaTGTTTTTTTTA.G.TT.G.TCT.T.G.TTT.TTGTTTT.TTTc                            <  1:834183/35‑1 (MQ=255)
        cttGTTTTTTTTT.G.TT.T.TTT.T.C.TTT.TTTtttt.tttt                            <  2:3000471/34‑2 (MQ=2)
        cgtGTCTTTTTTT.G.TT.T.TTT.T.G.TTT.TTTtttt.tttt                            <  2:2771148/34‑2 (MQ=0)
        aaTGTTTTTTTTA.G.TT.G.TCT.T.G.TTT.TTGtttt.tttt                            >  1:2248449/1‑35 (MQ=255)
         ttGTTTTTTTTT.T.TT.T.TCTCT.C.TTT.TTCtttt.tttt                            <  1:3451909/35‑2 (MQ=2)
         ttGTTTTTTTTT.T.TT.T.GCT.T.C.TTT.TTGtttt.tttta                           <  2:3376023/35‑3 (MQ=2)
         ttGTTTTTTTTT.G.TT.T.GTT.T.T.TTT.TTTtttt.tttgt                           <  2:3525672/35‑3 (MQ=0)
         ttGTTTTTTTTT.G.TG.T.TTT.T.T.TTG.TTGtttt.ttttt                           <  2:3635604/35‑3 (MQ=0)
         ttGTTTTTTTTT.G.GT.T.TGG.T.G.TTT.TTTtttt.ttttt                           <  2:3479566/35‑3 (MQ=0)
         ttGTTTTGTTTT.T.TT.T.TAT.T.T.TTT.TTGtttt.ttttt                           <  2:3525280/35‑3 (MQ=0)
         ttGTGTTTTTTT.G.TT.T.TTT.T.C.TTT.TTTtttt.ttttt                           <  2:3175584/35‑3 (MQ=2)
         gtGTTTTTTTTG.GTTT.T.TTT.T.G.TTG.TTTtttt.tttt                            <  2:3413809/35‑2 (MQ=2)
         gtGTTTTTTTTA.T.TT.T.TTT.T.T.CTT.TTTtttt.ttttt                           <  2:2730439/35‑3 (MQ=2)
         gtGTTTTGTTTA.T.TT.G.TTT.TCT.TTT.TTGtttt.tttt                            <  2:3626551/35‑2 (MQ=2)
         gtGTTTTCTTTT.G.TT.T.TTT.T.GTTTT.TTGtttt.tttt                            <  2:3117653/35‑2 (MQ=0)
         ctGTTGTTTTTC.G.TT.T.TTT.T.G.TGT.TTTtttt.ttttt                           <  1:3074505/35‑3 (MQ=0)
         aTGTTTTTTTTA.G.TT.G.TCT.T.G.TTT.TTGtttt.ttttt                           >  1:373022/1‑34 (MQ=255)
         aTGTTTTTTTTA.G.TT.G.TCT.T.G.TTT.TTGTTTT.TTTCt                           >  1:1883715/1‑36 (MQ=255)
         aTGTTTTTTTTA.G.TT.G.TCT.T.G.TTT.TTGTTTT.TTTCt                           <  1:2552385/36‑1 (MQ=255)
          tcttttttttG.T.TT.T.TTT.T.G.TTT.TTTTTTTATTTCt                           <  2:3497947/34‑1 (MQ=2)
          tGTTTTTTTTT.G.TT...TTT.T.G.TTT.TTTTTTT.TTTCttt                         <  2:3048620/36‑3 (MQ=0)
          tGTTTTTTTTG.T.TT.T.TTT.T.G.TTT.TTTTTTT.TTTCtt                          <  2:3561214/36‑2 (MQ=2)
          tGTTTTTGTTT.C.TT.G.TTT.T.T.TTT.TTTtttt.tttttt                          <  2:3493355/36‑4 (MQ=0)
          tGTTTGTTTTT.G.GT.G.TTT.T.T.ATT.TTGtttt.tttttt                          <  2:2870307/36‑4 (MQ=0)
          tGTTGTTTTTC.T.TT.T.TCT.T.T.TTT.TTTtttt.ttttct                          <  2:3098276/36‑4 (MQ=0)
          tGTCTTTTTTT.T.TT.T.TTT.T.T.TTT.TTGtttt.ttttct                          <  2:3243585/36‑4 (MQ=2)
           ttttttttgT.G.TT.GTTGT.T.T.TTT.TTGTTTT.TTTCtt                          <  2:3580975/35‑2 (MQ=2)
           tttttttctT.T.TT.G.TTT.T.GTTTT.TTGTTTT.TTTCtt                          <  2:3290979/35‑2 (MQ=12)
           tttttttctC.G.TT...TCT.T.C.TTT.TT.TTTT.TTTCTTttt                       <  1:3479325/35‑1 (MQ=0)
           gTTTTTTTTA.G.TT.G.TCT.T.G.TTT.TTGTTTT.TTTCttt                         >  1:311559/1‑34 (MQ=255)
           gTTTTTTTTA.G.TT.G.TCT.T.G.TTT.TTGTTTT.TTTCTAt                         <  2:2498419/36‑1 (MQ=255)
           gTTCTTTTTG.G.TTCG.TGT.T.T.TTT.TTGtttt.tttttt                          <  2:3451252/36‑4 (MQ=0)
            ttttttttT.T.TT.T.TCT.T.T.AAT.TTGTTTT.TTTCTTtt                        <  2:3562546/36‑1 (MQ=2)
            ttttttttT.T.TT.G.T.T.T.G.TTT.TT.TTTT.TTTCTCtttt                      <  2:2831095/36‑2 (MQ=2)
            ttttttttG.T.TT.G.T.T.T.G.TTT.TT.TTTT.TTTCTTtttt                      <  2:2730337/36‑2 (MQ=0)
            ttttttttC.G.TT...TTT.T.G.TTC.TTTTTTT.TTTCTTttt                       <  2:3580670/36‑1 (MQ=2)
            ttttttttA...TG.A.TCT.T.G.TTT.GTTTTTT.TTTTTTttt                       <  2:3590181/36‑1 (MQ=0)
            ttttgtttT.G.TT...TCT.T.G.TTT.TTTTTTT.GTTTTTttt                       <  2:3272149/36‑1 (MQ=2)
            tttgttttA.G.TT...TGT.T.G.TTT.TTTTTTT.TTTTTTttt                       <  2:3705812/36‑1 (MQ=2)
            tgttttttT.T.TT.G.T.T.T.C.TTT.TTTTTTT.TTTCTTttt                       <  2:2950219/34‑1 (MQ=2)
             tttttttT.G.TT...TTT.T.G.TTT.TT.TTTT.TTTGTAttttt                     <  2:2741429/36‑3 (MQ=9)
             tttttttA.G.TT.G.TCT.T.G.TTT.TTGTTTT.TTTTTAttt                       >  2:3395849/1‑36 (MQ=255)
             ttgttttT.G.TT.C.TTT.T.T.TTT.TTGTTTT.TTTTTTttt                       <  1:3368036/33‑1 (MQ=0)
             tggttttT.G.TT.G.TTT.T.G.TTT.TTTTTTT.TTTTTTttt                       <  2:3366436/33‑1 (MQ=0)
             gttttttT.T.TT.G.TTG.T.T.TTT.TTGTTTT.TTTTTTttt                       <  2:2719734/35‑1 (MQ=0)
             gttttttG.T.TT.T.TGT.T.G.TTT.TTTTTTT.TTTTTTttt                       <  2:3233770/35‑1 (MQ=0)
             gttttttC.T.TT.T.TGT.T.G.TTT.TT.TTTT.TTTCTCtttt                      <  2:3233853/35‑2 (MQ=2)
             gttttctA.G.TT.G...T.T.G.TTT.TTGTTTT.TTTTTTttttt                     <  2:3185326/35‑3 (MQ=2)
              ttttttT.T.TT.C.TGT.T.C.TTT.TTGTTTT.TTTTTCtttt                      <  2:3156278/36‑2 (MQ=2)
              ttttttT.G.TT.T.TGT.G.T.TTT.TTGTTTT.TTTTTTtttt                      <  2:3442072/36‑2 (MQ=0)
              ttttttA.G.TG.C.TTT.T.G.TTT.TTATTTA.TTTATTtttt                      <  2:2821279/36‑2 (MQ=0)
              tttttcT.G.TT.T.TTT.T.G.TTT.TTTTTTC.TTTTTAttgt                      <  2:3029695/36‑3 (MQ=0)
              tttttcT.G.TA.G.TTT.T.G.TTT.TTTTTTT.TTTTTTtttt                      <  2:3544286/36‑2 (MQ=0)
              ttttatA.T.TT.T.TTT.T.G.TTT.TTTGTTT.TTTTTAtttt                      <  2:3194733/36‑2 (MQ=0)
              ttgtttA.G..T.G.TGT.T.G.TTTGTTGTTTT.TTTTTTTTTc                      <  2:3752070/33‑1 (MQ=0)
              gtttttC.G.TT.T.TTT.T.T.TTT.TTGTTTT.TTTTTTtttt                      <  2:2781206/35‑2 (MQ=2)
               tttttT.T.TT.G.TTT.T.G.TTT.GTTTTTT.TTTCTTttttt                     <  2:3717108/36‑3 (MQ=0)
               tttttA.T.TT.G.TTG.T.T.TTT.TTGTTTT.TTTTTTttttt                     <  2:3300394/36‑3 (MQ=2)
               tttttA.G.TT.G.TTC.T.T.TTT.TTGTCTT.TTGTTAttttt                     <  2:3137025/36‑3 (MQ=0)
               tttttA.G.TT.G.TCT.T.G.TTT.TTGTTTT.TTTCTATTTct                     >  1:2475464/1‑35 (MQ=255)
               tttttA.G.TT.G.TCT.T.G.TTT.TTGTTTT.TTTCTATTTct                     >  2:3310157/1‑35 (MQ=255)
                ttttT.G.TT.G.TCT.G.T.TTT.TTTTTTT.TTTTTTtttttt                    <  2:2911147/36‑4 (MQ=0)
                ttttAGG.TT.G.TTT.T.T.CTT.TTGTTTT.TTTTTTttttt                     <  2:2945409/36‑3 (MQ=2)
                 tttA.G.TT.G.TCT.T.G.TTT.TTGTTTT.TTTCTATTTCGCa                   <  1:974633/36‑1 (MQ=255)
                 tttA.G.TT.G.TCT.T.G.TTT.TTGTTTT.TTTCTATTTCGCa                   <  2:1285665/36‑1 (MQ=255)
                  ttA.G.TT.G.TCT.T.G.TTT.TTGTTTT.TTTCTATTTCTCaa                  >  2:3645837/1‑36 (MQ=255)
                   tA.G.TT.G.TCT.T.G.TTT.TTGTTTT.TTTCTATTTCGCAat                 <  2:360651/36‑1 (MQ=255)
                      g.TT.G.TCT.T.G.TTT.TTGTTTT.TTTCTATTTCGCAatat               <  2:800935/36‑1 (MQ=255)
                        tt.G.TCT.T.G.TTT.TTGTTTG.TTTCTATTTCGCAATAtt              <  2:2683406/36‑1 (MQ=255)
                         t.G.TCT.T.G.TTT.TTGTTTT.TTTCTATTTCGCAATATTa             <  1:154748/36‑1 (MQ=255)
                           g.TCT.T.G.TTT.TTGTTTT.TTTCTATTTCGCAATATTaa            >  2:670225/1‑36 (MQ=255)
                             tCT.T.G.TTT.TTGTTTT.TTTCTATTTCGCAATATTAAt           >  1:3607742/1‑36 (MQ=255)
                              cT.T.G.TTT.TTGTTTT.TTTCTATTTCGCAATATTAATg          >  1:3386959/1‑36 (MQ=255)
                              cT.T.G.TTT.TTGTTTT.TTTCTATTTCGCAATATTAATg          >  2:926368/1‑36 (MQ=255)
                                       t.ttgtttt.tTTCTATTTCGCAATATTAATGACTgtg    >  2:1740765/1‑36 (MQ=255)
                                       t.ttgtttt.tTTCTATTTCGCAATATTAATGACAgtg    >  1:2967728/1‑36 (MQ=255)
                                           |                                   
CTGGTGGGAATGTTTTTTTTA.G.TT.G.TCT.T.G.TTT.TTGTTTT.TTTCTATTTCGCAATATTAATGACAGTGTC  >  REL606/4462971‑4463040

Base quality scores:  ATCG  < 3 ≤  ATCG  < 5 ≤  ATCG  < 10 ≤  ATCG  < 21 ≤  ATCG  < 32 ≤  ATCG