Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6063,357,0100C→T53.5% 117.6161intergenic (‑343/‑131)rrsD/yrdA16S ribosomal RNA/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/76):  ref base (17/50):  total (18/143)

Only 100 of 162 total aligned reads displayed.

AATTTTTTGCACAAGTATTTTTTTGA.TCT.T.T.TT.T.T.C.TGT.TTGTTGTTTTTTCACCCTTTTTGCTGCAATC  >  REL606/3356975‑3357044
                                          |                                    
aaTTTTTTGCACAAGTATTTTTTTGA.TCT.T.T.TT.T.T.c                                      <  2:2799994/36‑1 (MQ=255)
 aTTTTTTGCACAAGTATGGTTTTGA.TCT.T.T.TT.T.T.C.t                                    <  2:89679/36‑1 (MQ=37)
  ttttttGCACAAGTATTTTTTTGA.TCT.T.T.TT.T.T.C.tg                                   <  2:2706947/36‑1 (MQ=255)
   tttttGCACAAGTATTTTTTTGA.TCT.T.T.TT.T.T.C.tgt                                  >  1:3508622/1‑36 (MQ=255)
    ttttGCACAAGTATTTTTTTGA.TCT.T.T.TT.T.T.C.tgt.t                                >  2:3667148/1‑36 (MQ=255)
       tGCACAAGTATTTTTTTGA.TCT.T.T.TT.T.T.C.tgt.ttgt                             <  1:313966/36‑1 (MQ=255)
        gCACAAGTATTTTTTTGA.TCT.T.T.TT.T.T.C.tgt.ttgtt                            <  1:1120976/36‑1 (MQ=255)
        gCACAAGTATTTTTTTGA.TCT.T.T.TT.T.T.C.tgt.ttgtt                            <  1:1817832/36‑1 (MQ=255)
         cacaAGTATTTTTTTGA.TCT.T.T.TT.T.T.C.TGT.ttgttg                           <  2:1861217/36‑1 (MQ=255)
           cgaGTATTTTTTTGA.TCT.T.T.TT.T.T.C.TGT.TTgttgtt                         <  1:2529049/34‑1 (MQ=255)
           caAGTATTTTTTTGA.TCT.T.T.TT.T.T.C.TGT.TTgttgtt                         >  2:2964271/1‑36 (MQ=255)
           caAGTATTTTTTTGA.TCT.T.T.TT.T.T.C.TGT.TTgttgtt                         >  2:150308/1‑36 (MQ=255)
            ttttATCTTTTTTT.TCT.T.T.TT.T.T.C.T.T.TTTTTGtttt                       <  2:3432939/33‑1 (MQ=2)
            ttgTATTTTTTTCT.TTT.T.T.TT.T.T.T.TTT.TTCTTTttt                        <  2:3166042/34‑1 (MQ=2)
            tgttATATTTTCTA.TCT.T.T.TT.T.T...TTT.TTTTTGtttt                       <  2:3020135/33‑1 (MQ=9)
            gtttATTTTTTTTA.TTT.T.T.TT.C.T.T.TTT.TTGTTTttt                        <  2:3488730/33‑1 (MQ=0)
            gaGTATTTTTTGGT.TTT.T.T.TT.T.T.T.TTT.TTTTTTttt                        <  1:2819957/35‑1 (MQ=2)
            ctttATTTTTTTGT.TTT.T.T.TT.T.T.T.TCT.TTTTTTttt                        <  2:3309762/33‑1 (MQ=0)
            atttATTTTTCTTT.TCT.T.T.TT.T.T.C.TTT.TTTTTTttt                        <  2:3068967/33‑1 (MQ=2)
            aaGTATTTTTTTGA.TCT.T.T.TT.T.T.C.TGT.TTGTTGttt                        >  2:3378355/1‑36 (MQ=255)
            aaGTATTTTTTTGA.TCT.T.T.TT.T.T.C.TGT.TTGTTGttt                        >  2:1905400/1‑36 (MQ=255)
             tttATTTTTTTGT.TTT.T.G.TT.T.T.G.TTT.TTTTTTtttt                       <  2:3635176/34‑1 (MQ=2)
             tttATCTTTTTTT.TCT.T.T.TT.T.T.C.TTT.TTTTTTtttt                       <  2:3328797/34‑1 (MQ=2)
             tgTATTTTTTT.A.T.T.T.T.TC.T.T.T.TTT.TTGTTTtttttt                     <  1:3006721/35‑1 (MQ=9)
             gttATTTTTTTGT.TTA.T.T.TT.T.T.T.TTT.TTTTTTtttt                       <  2:2960734/34‑1 (MQ=9)
              ttttttttttCT.TCT.T.T.TT.G.T.T.TTT.TTGTTTttttt                      <  2:2940322/33‑1 (MQ=2)
              ttttttttgtGT.TTT.G.T.TT.T.T.T.TGT.TTGTTGttttt                      <  2:2841205/33‑1 (MQ=9)
              ttgtttttttTA.TCT.T.T.TT.T.T.C.TTT.TTTTTTttttt                      <  2:3460934/33‑1 (MQ=12)
              ttctttttttG..TTT.T.T.TT.G.T.C.TTT.TTTTTTtttttt                     <  2:3243433/33‑1 (MQ=0)
              ttcttttattGT.TTT.T.T.TT.T.T.T.TGT.TTTTTTttttt                      <  2:3690089/33‑1 (MQ=2)
              tcttttttttGA.T.T.T.T.TC.T.T.C.TTT.TNTTTTtttttt                     <  2:3590180/33‑1 (MQ=2)
              tcgtttttttG..TTT.T.T.TT.T.T.C.TTT.TTTTTTtttttt                     <  2:3049520/33‑1 (MQ=2)
              gtgtttttttG..TTT.T.T.TT.T.T.T.TTT.TTCTTTtttttt                     <  2:3689767/33‑1 (MQ=0)
              gTATTTTTTTCA.TCT.T.T.TT.T.T.C.TGT.TTGTTGttttt                      >  2:2030423/1‑36 (MQ=255)
              ctttttttttTC.TCT.T.T.TT.T.T...TCT.TTTTTTtttttt                     <  2:3507277/33‑1 (MQ=2)
              ctttttgtttGA..CT.T.C.TT.T.T.C.TTT.TTTTTTtttttt                     <  2:3347774/33‑1 (MQ=2)
              ctgtttttttGA.TTT.T.T.TT.T.T.T.TTT.TTTTTTttttt                      <  1:2760623/33‑1 (MQ=9)
               tttttttttGT.TCT.T.T.TT.T.T.T.TTT.TTATTTcttttt                     <  2:3233956/34‑1 (MQ=2)
               tttttttttGT.TCT.T.A.TT.G.T.C.TTG.TTTTTTtttttt                     <  2:3059305/34‑1 (MQ=2)
               tttttatttTA.TTT.T.T.TT.T.T.C.TTT.TTTTTGtttttt                     <  2:3291089/34‑1 (MQ=2)
               tgtttttttGT.TTT.T.T.TT.T.T.C.TTT.TTTTTTttcttt                     <  1:3293463/34‑1 (MQ=2)
               tgtttttttGT.TTT.T.T.TT.G.T.T.TTT.TTTTTGtttttt                     <  2:3534823/34‑1 (MQ=2)
               tgtttttttGT.TTT.C.T.TT.T.T.T.TTT.TTTTTCtttttt                     <  2:3137028/34‑1 (MQ=9)
               tgtttttttGT.TGT.T.T.TT.T.T.G.TTT.TTCTGTtttttt                     <  2:2789881/34‑1 (MQ=2)
               tgtttttttCT.TGT.G.T.TT.T.T.T.TGT.TTTTTGttgttt                     <  2:3337638/34‑1 (MQ=2)
               tgtttatttGT.TTT.T.T.TT.T.T.T.TCT.TTTTTTtttttt                     <  2:3725818/34‑1 (MQ=2)
               tctttttttTT.TCT.T.T.TC.T.T.T.TTT.TTCTTTtttttt                     <  1:2770592/34‑1 (MQ=2)
               tcttttgttTA.TCT.T.T.TT.T.T.T.TTT.TTTTTTtttttt                     <  1:3451911/34‑1 (MQ=2)
               tATTTTGTTTT.TTT.T.T.TT.T.T.C.TTT.TTTTTCtttttt                     <  1:2869467/36‑1 (MQ=2)
               tATTTTATTTT.TCT.T.T.TT.T.T.T.TTT.TTTTTCtttttt                     <  1:3561323/36‑1 (MQ=0)
               gttttttgtGT.TTT.T.T.TT.T.T.C.T.T.TTGTTTttttttt                    <  2:3291132/34‑2 (MQ=2)
               gtttttcttGA.TCT.T.T.TT.T.T.T.TTT.TTTTTTtttttt                     <  1:3730625/34‑1 (MQ=9)
               gtgttttttTT.TCT.T.T.TT.T.T.T.TGT.TTTTTTtttttt                     <  2:3507140/33‑1 (MQ=0)
               gtgttttttGT.TTT.T.T.TT.T.T.C.TTG.TTTTTTtttttt                     <  1:2720006/33‑1 (MQ=0)
               gaTTTTGTTTT.TCT.T.T.TT.T.T.T.TTT.TTTTTTtttttt                     <  2:2778035/35‑1 (MQ=0)
               cttttttttTG.TTT.T.T.CT.T.T.C.T.T.TTGTTTttttttt                    <  2:3608489/34‑2 (MQ=2)
               ctttttcttGT.TGT.T.T.TT.T.T.T.TTT.TTCTTTtttttt                     <  2:2870397/34‑1 (MQ=2)
               cttttatttTA.TTT.T.T.TT.T.T.T.TGT.TTTGTTtttttt                     <  2:3224270/34‑1 (MQ=2)
               caTTTTTTTCT.TCT.T.TCTT.T.T.T.TTT.TTTTTGttttt                      <  2:3498076/35‑1 (MQ=2)
               attttttttTC.TAT.T.T.TT.C.T.T.TTT.TTTTTGtttttt                     <  2:3717015/34‑1 (MQ=0)
                ttttttttTT.TTT.T.T.TT.T.T.G.TTG.TTGTTTttttttg                    <  2:3078788/35‑2 (MQ=2)
                ttttttttGC.TTTCT.T.TT.T.T.T.TCT.TTTTTGtttttt                     <  2:3253044/35‑1 (MQ=0)
                ttttttttGA.TGT.T.T.TTCT.T.T.TCT.TTTTTTtttttt                     <  1:3405735/35‑1 (MQ=0)
                ttttttttCT.TTT.T.T.TT.T.T.T.TGTGTTTTTGtttttt                     <  2:3127379/35‑1 (MQ=2)
                ttttttgtGT.TTT.T.T.GT.T.T.T.TTT.TTGTTTttttttt                    <  2:3511843/35‑2 (MQ=2)
                ttttttgtGT.TTT.G.T.TT.T.T.T.TGT.TTTTTTttttttt                    <  2:3214593/35‑2 (MQ=2)
                ctttttttG..TTT.T.T.TT.T.T.C.TTT.TTTTTTTTTTTTct                   <  2:3525816/35‑2 (MQ=2)
                ccttttttGT.TTT.T.T.TT.T.T.T.TGT.TTTTTTttttttt                    <  1:3668973/34‑2 (MQ=2)
                aTTTTTTTTT.TCT.T.T.TT.T.T.T.TCT.TTCTTTTTTTCTc                    >  2:639598/1‑36 (MQ=2)
                aTTTTTTTGA.TTG.T.T.TT.T.T.T.TTT.TCTTTCttttttt                    <  2:3414295/36‑2 (MQ=2)
                aTTTTTTTGA.TCT.T.T.TT.T.T.C.TGT.TTGTTGTTTTTTc                    <  2:1489260/36‑1 (MQ=255)
                 tttttttTT.TTT.T.T.TT.T.T.CTTGT.TTGTTCttttttt                    <  2:3544213/36‑2 (MQ=2)
                 tttttttTT.TTT.T.G.TT.T.T.CTTTT.TTGTTGttttttt                    <  2:3039719/36‑2 (MQ=2)
                 tttttttTT.TCT.T.T.TT.T.T.T.CTT.TTCTTTtttttttc                   <  2:3570510/36‑3 (MQ=2)
                 tttttttTG.TTT.T.T.TT.T.T.C.TTT.TTGTTCTTTTTTct                   <  2:3338294/36‑2 (MQ=12)
                 tttttttTG.TTT.T.T.TT.G.T.T.TTT.TTGTTGttgtttgt                   <  2:3020148/36‑3 (MQ=2)
                 tttttttTA.TTT.T.G.TT.T.T.C.TGT.TTTTTTtttttttt                   <  2:3562518/36‑3 (MQ=2)
                 tttttttGT.TTT.T.T.TT.T.T.T.TTT.TTCTTTTTTCTTct                   <  2:2910943/36‑2 (MQ=2)
                 tttttttGA.TTT.T.T.TT.TGT.C.TTT.TTTTTTttttttt                    <  1:3615383/36‑2 (MQ=9)
                 tttttttCT.TCT.T.T.TT.T.T...TCT.TTATTTttttttttt                  <  1:2800152/36‑4 (MQ=2)
                 ttttttaGT.TTT.G.T.TT.T.T.T.TTT.TTATTGtttttttt                   <  2:3752084/36‑3 (MQ=9)
                 tttttgtGT.TTT.T.T.TT.T.T.C.TTT.CTTTTTtttttttt                   <  2:2920789/36‑3 (MQ=2)
                 ttttgttGA.TGT.T.T.TT.T.T.T.TTT.CTTTTTtttttttt                   <  2:3734494/36‑3 (MQ=2)
                 tttgtttTA.TCT.T.T.TT.T.T.T.TCT.TTTTTTtttttttt                   <  2:3010165/36‑3 (MQ=0)
                 tttctttTA.TGT.T.T.TT.T.T.T.AGG.TTGTTTtttttttt                   <  2:3506843/36‑3 (MQ=2)
                 gttttttTC.TCT.C.T.TT.T.T.T.TGT.TTTTTTtttttttt                   <  2:2750857/35‑3 (MQ=2)
                 gttttttGT.TCT.T.T.TT.TCT.T.TGT.TTTTTTttttttt                    <  2:3020198/35‑2 (MQ=2)
                 gtttgttGC.TTT.T.G.TT.T.T.T.TCT.TTGTTGtttttttt                   <  2:3423338/35‑3 (MQ=0)
                 cttttttCG.TCT.T.T.TT.T.T.T.TTT.TTTTTTtttttttt                   <  2:3690010/35‑3 (MQ=0)
                  ttttttTT.TTT.T.T.TT.T.T.T.TTT.TTGTTTTTTTTGCaa                  <  2:2937082/36‑2 (MQ=2)
                  ttttttGT.TTT.TGT.TT.T.T.C.T.T.TTGTTTttttttttt                  <  2:3732058/36‑4 (MQ=2)
                  tttcttGT.TGT.T.T.TT.T.T.T.TGT.TTTTTTttttttttt                  <  2:3281766/36‑4 (MQ=2)
                  ctttttGAGTTT.T.T.TT.T.T.T.TTT.TTGTTGtttttttt                   <  2:2888885/35‑3 (MQ=2)
                   tttttGT.TTT.G.T.TT.T.T.T.TGTGTTGTTGtttttttgg                  <  2:3127326/36‑4 (MQ=0)
                   tttttGT.TCT.T.T.TT.T.TGG.TGT.TTTTTCttttttttt                  <  2:3608448/36‑4 (MQ=2)
                   tttttGA.TCT.T.T.TT.T.T.C.TGT.TTGTTGTTTTTTCatc                 >  2:2970731/1‑34 (MQ=255)
                   tttttGA.TCT.T.T.TT.T.T.C.TGT.TTGTTGTTTTTTCAcc                 >  2:842307/1‑36 (MQ=255)
                       tGA.TCT.T.T.TT.T.T.C.TGT.TTGTTGTTTTTTCACCCttt             >  1:2114620/1‑36 (MQ=255)
                                      t.t.C.TGT.TTGTTGTTTTTTCACCCTTTTTGCTGCAAt   >  2:372498/1‑36 (MQ=255)
                                        t.C.TGT.TTGTTGTTTTTTCGCCCTTTTTGCTGCAATc  <  1:879231/36‑1 (MQ=255)
                                          |                                    
AATTTTTTGCACAAGTATTTTTTTGA.TCT.T.T.TT.T.T.C.TGT.TTGTTGTTTTTTCACCCTTTTTGCTGCAATC  >  REL606/3356975‑3357044

Base quality scores:  ATCG  < 3 ≤  ATCG  < 5 ≤  ATCG  < 9 ≤  ATCG  < 19 ≤  ATCG  < 32 ≤  ATCG