LOCUS       phusion_pE              7800 bp    DNA     circular SYN 11-MAY-2015
DEFINITION  Ligation of pet16 frag into pet28 vec.
ACCESSION   phusion_pE
KEYWORDS    .
SOURCE      Unknown
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 7800)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational
            Software.
FEATURES             Location/Qualifiers
     CDS             complement(12..467)
                     /gene="f1"
     CDS             complement(560..1375)
                     /gene="kanR"
     CDS             complement(2083..2084)
                     /gene="pBR322 ori"
     CDS             complement(3515..4474)
                     /gene="lacI"
     CDS             5071..7668
                     /gene="6his-pfu-sso7d"
ORIGIN      
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
      601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
      661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
      721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
      781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
      841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
      901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
      961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
     1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
     2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
     3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
     4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
     4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
     5041 ttttgtttaa ctttaagaag gagatatacc atgggccatc atcatcatca tcatcatcat
     5101 catcacagca gcggccatat cgaaggtcgt catatgattt tagatgtgga ttacataact
     5161 gaagaaggaa aacctgttat taggctattc aaaaaagaga acggaaaatt taagatagag
     5221 catgatagaa cttttagacc atacatttac gctcttctca gggatgattc aaagattgaa
     5281 gaagttaaga aaataacggg ggaaaggcat ggaaagattg tgagaattgt tgatgtagag
     5341 aaggttgaga aaaagtttct cggcaagcct attaccgtgt ggaaacttta tttggaacat
     5401 ccccaagatg ttcccactat tagagaaaaa gttagagaac atccagcagt tgtggacatc
     5461 ttcgaatacg atattccatt tgcaaagaga tacctcatcg acaaaggcct aataccaatg
     5521 gagggggaag aagagctaaa gattcttgcc ttcgatatag aaaccctcta tcacgaagga
     5581 gaagagtttg gaaaaggccc aattataatg attagttatg cagatgaaaa tgaagcaaag
     5641 gtgattactt ggaaaaacat agatcttcca tacgttgagg ttgtatcaag cgagagagag
     5701 atgataaaga gatttctcag gattatcagg gagaaggatc ctgacattat agttacttat
     5761 aatggagact cattcgactt cccatattta gcgaaaaggg cagaaaaact tgggattaaa
     5821 ttaaccattg gaagagatgg aagcgagccc aagatgcaga gaataggcga tatgacggct
     5881 gtagaagtca agggaagaat acatttcgac ttgtatcatg taataacaag gacaataaat
     5941 ctcccaacat acacactaga ggctgtatat gaagcaattt ttggaaagcc aaaggagaag
     6001 gtatacgccg acgagatagc aaaagcctgg gaaagtggag agaaccttga gagagttgcc
     6061 aaatactcga tggaagatgc aaaggcaact tatgaactcg ggaaagaatt ccttccaatg
     6121 gaaattcagc tttcaagatt agttggacaa cctttatggg atgtttcaag gtcaagcaca
     6181 gggaaccttg tagagtggtt cttacttagg aaagcctacg aaagaaacga agtagctcca
     6241 aacaagccaa gtgaagagga gtatcaaaga aggctcaggg agagctacac aggtggattc
     6301 gttaaagagc cagaaaaggg gttgtgggaa aacatagtat acctagattt tagagcccta
     6361 tatccctcga ttataattac ccacaatgtt tctcccgata ctctaaatct tgagggatgc
     6421 aagaactatg atatcgctcc tcaagtaggc cacaagttct gcaaggacat ccctggtttt
     6481 ataccaagtc tcttgggaca tttgttagag gaaagacaaa agattaagac aaaaatgaag
     6541 gaaactcaag atcctataga aaaaatactc cttgactata gacaaaaagc gataaaactc
     6601 ttagcaaatt ctttctacgg atattatggc tatgcaaaag caagatggta ctgtaaggag
     6661 tgtgctgaga gcgttactgc ctggggaaga aagtacatcg agttagtatg gaaggagctc
     6721 gaagaaaagt ttggatttaa agtcctctac attgacactg atggtctcta tgcaactatc
     6781 ccaggaggag aaagtgagga aataaagaaa aaggctctag aatttgtaaa atacataaat
     6841 tcaaagctcc ctggactgct agagcttgaa tatgaagggt tctataagag gggattcttc
     6901 gttacgaaga agaggtatgc agtaatagat gaagaaggaa aagtcattac tcgtggttta
     6961 gagatagtta ggagagattg gagtgaaatt gcaaaagaaa ctcaagctag agttttggag
     7021 acaatactaa aacacggaga tgttgaagaa gctgtgagaa tagtaaaaga agtaatacaa
     7081 aagcttgcca attatgaaat tccaccagag aagctcgcaa tatatgagca gataacaaga
     7141 ccattacatg agtataaggc gataggtcct cacgtagctg ttgcaaagaa actagctgct
     7201 aaaggagtta aaataaagcc aggaatggta attggataca tagtacttag aggcgatggt
     7261 ccaattagca atagggcaat tctagctgag gaatacgatc ccaaaaagca caagtatgac
     7321 gcagaatatt acattgagaa ccaggttctt ccagcggtac ttaggatatt ggagggattt
     7381 ggatacagaa aggaagacct cagataccaa aagacaagac aagtcggcct aacttcctgg
     7441 cttaacatta aaaaatccgg taccggcggt ggcggtgcaa ccgtaaagtt caagtacaaa
     7501 ggcgaagaaa aagaggtaga catctccaag atcaagaaag tatggcgtgt gggcaagatg
     7561 atctccttca cctacgacga gggcggtggc aagaccggcc gtggtgcggt aagcgaaaag
     7621 gacgcgccga aggagctgct gcagatgctg gagaagcaga aaaagtgata actgctaaca
     7681 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
     7741 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
//
