Barrick Lab :: ProtocolsRecipesTetrazoliumSugar

---+++ TA: Tetrazolium Sugar (TA, TM, TL, ...)


Combine in a 1 L flask:
| *500 mL* | *Component* |
| 5 g | Tryptone | 
| 500 mg | Yeast Extract |
| 2.5 g | NaCl | 
| 8 g | Agar | 
| 500 µL | 5% Antifoam | 
Add dH<sub>2</sub>O to 433.33 mL.

Combine in a 125 ml flask:
|  *500 mL*  |  *Component*  |
| 5 g | Sugar (arabinose = TA, maltose = TM, lactose = TL) | 
Add dH<sub>2</sub>O to 66.67 mL.

Cover each flask with foil, and autoclave sugar and media separately.
Total combined volume will be 500 mL.

Combine the autoclaved sugar and media solutions and add:
|  *500 mL*  |  *Component*  |
| 500 µL | 5% (w/v) Triphenyl Tetrazolium chloride (TTC), filter sterilized, stored at 4°C |

Source: [[http://myxo.css.msu.edu/ecoli/taagar.html][Lenski Lab Protocol]]

Barrick Lab > ProtocolsMediaRecipes > ProtocolsRecipesTetrazoliumSugar

Contributors to this topic

CraigBarnhart, JeffreyBarrick, AlexaMorton, JordanMonk

Topic revision: r8 - 2025-01-09 - 20:04:48 - Main.AlexaMorton