Combine in a 1 L flask:
| 500 mL | Component |
|---|---|
| 5 g | Tryptone |
| 500 mg | Yeast Extract |
| 2.5 g | NaCl |
| 8 g | Agar |
| 500 µL | 5% Antifoam |
Combine in a 125 ml flask:
| 500 mL | Component |
|---|---|
| 5 g | Sugar (arabinose = TA, maltose = TM, lactose = TL) |
Cover each flask with foil, and autoclave sugar and media separately. Total combined volume will be 500 mL.
Combine the autoclaved sugar and media solutions and add:
| 500 mL | Component |
|---|---|
| 500 µL | 5% (w/v) Triphenyl Tetrazolium chloride (TTC), filter sterilized, stored at 4°C |
Source: Lenski Lab Protocol